Genotoxic evidences of glyphosate and chlorpyriphos on eisenia fetida coelomocytes

Autores
Curieses, Silvana Patricia; Sáenz, María Elena; Alberdi, José Luis; Martinez, Ricardo Santiago; Larramendy, Marcelo Luis; Di Marzio, Walter Dario
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The organophosphorus herbicide glyphosate (GLY) and the organophosphate insecticide chlorpyriphos (CPF) are key pesticides in modern management cultures worldwide. Sublethal toxicity of the commercial herbicide formulation Roundup® and the insecticide formulation Terfos® were evaluated on Eisenia fetida coelomocytes exposed under in vivo and ex vivo laboratory conditions. Induction of DNA single-strand breaks evaluated by the single cell gel electrophoresis assay and coelomocyte viability as well as alterations in coelomocyte trophic indexes were employed as endpoints for genotoxicity and cytotoxicity, respectively. Specimens were exposed at concentrations corresponding to recommended pesticide field application rate, and endpoints were evaluated after 7 and 14 days of treatment (in vivo exposure). In addition, coelomocytes were exposed to aqueous leachate of pesticide-contaminated soils during 1 h (ex vivo exposure). Earthworms exposed to Roundup® and Terfos® showed an increased frequency of DNA damage. Also, a decrease of coelomocyte viability and decrease of trophic indexes were observed in all treatments. The results demonstrate that either GLY- and CPF-based formulations exerted genotoxic as well as cytotoxic effects in coelomocytes of E. fetida exposed in vivo and ex vivo.
Fil: Curieses, Silvana Patricia. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sáenz, María Elena. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alberdi, José Luis. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina
Fil: Martinez, Ricardo Santiago. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Di Marzio, Walter Dario. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
DNA DAMAGE
HERBICIDES
INSECTICIDES
SINGLE CELL GELL ELECTROFORESIS ASSAY
COMMERCIAL FORMULATIONS
SUBLETHAL EFFECTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/131594
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/131594
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genotoxic evidences of glyphosate and chlorpyriphos on eisenia fetida coelomocytesCurieses, Silvana PatriciaSáenz, María ElenaAlberdi, José LuisMartinez, Ricardo SantiagoLarramendy, Marcelo LuisDi Marzio, Walter DarioDNA DAMAGEHERBICIDESINSECTICIDESSINGLE CELL GELL ELECTROFORESIS ASSAYCOMMERCIAL FORMULATIONSSUBLETHAL EFFECTShttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1The organophosphorus herbicide glyphosate (GLY) and the organophosphate insecticide chlorpyriphos (CPF) are key pesticides in modern management cultures worldwide. Sublethal toxicity of the commercial herbicide formulation Roundup® and the insecticide formulation Terfos® were evaluated on Eisenia fetida coelomocytes exposed under in vivo and ex vivo laboratory conditions. Induction of DNA single-strand breaks evaluated by the single cell gel electrophoresis assay and coelomocyte viability as well as alterations in coelomocyte trophic indexes were employed as endpoints for genotoxicity and cytotoxicity, respectively. Specimens were exposed at concentrations corresponding to recommended pesticide field application rate, and endpoints were evaluated after 7 and 14 days of treatment (in vivo exposure). In addition, coelomocytes were exposed to aqueous leachate of pesticide-contaminated soils during 1 h (ex vivo exposure). Earthworms exposed to Roundup® and Terfos® showed an increased frequency of DNA damage. Also, a decrease of coelomocyte viability and decrease of trophic indexes were observed in all treatments. The results demonstrate that either GLY- and CPF-based formulations exerted genotoxic as well as cytotoxic effects in coelomocytes of E. fetida exposed in vivo and ex vivo.Fil: Curieses, Silvana Patricia. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sáenz, María Elena. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alberdi, José Luis. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; ArgentinaFil: Martinez, Ricardo Santiago. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Di Marzio, Walter Dario. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaScholars.Direct2018-07-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131594Curieses, Silvana Patricia; Sáenz, María Elena; Alberdi, José Luis; Martinez, Ricardo Santiago; Larramendy, Marcelo Luis; et al.; Genotoxic evidences of glyphosate and chlorpyriphos on eisenia fetida coelomocytes; Scholars.Direct; Advances in Environmental Studies; 2; 2; 6-7-2018; 82-902642-4231CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.36959/742/206info:eu-repo/semantics/altIdentifier/url/https://scholars.direct/Articles/environmental-studies/aes-2-011.php?jid=environmental-studiesinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:11Zoai:ri.conicet.gov.ar:11336/131594instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:11.546CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genotoxic evidences of glyphosate and chlorpyriphos on eisenia fetida coelomocytes
title Genotoxic evidences of glyphosate and chlorpyriphos on eisenia fetida coelomocytes
spellingShingle Genotoxic evidences of glyphosate and chlorpyriphos on eisenia fetida coelomocytes
Curieses, Silvana Patricia
DNA DAMAGE
HERBICIDES
INSECTICIDES
SINGLE CELL GELL ELECTROFORESIS ASSAY
COMMERCIAL FORMULATIONS
SUBLETHAL EFFECTS
title_short Genotoxic evidences of glyphosate and chlorpyriphos on eisenia fetida coelomocytes
title_full Genotoxic evidences of glyphosate and chlorpyriphos on eisenia fetida coelomocytes
title_fullStr Genotoxic evidences of glyphosate and chlorpyriphos on eisenia fetida coelomocytes
title_full_unstemmed Genotoxic evidences of glyphosate and chlorpyriphos on eisenia fetida coelomocytes
title_sort Genotoxic evidences of glyphosate and chlorpyriphos on eisenia fetida coelomocytes
dc.creator.none.fl_str_mv Curieses, Silvana Patricia
Sáenz, María Elena
Alberdi, José Luis
Martinez, Ricardo Santiago
Larramendy, Marcelo Luis
Di Marzio, Walter Dario
author Curieses, Silvana Patricia
author_facet Curieses, Silvana Patricia
Sáenz, María Elena
Alberdi, José Luis
Martinez, Ricardo Santiago
Larramendy, Marcelo Luis
Di Marzio, Walter Dario
author_role author
author2 Sáenz, María Elena
Alberdi, José Luis
Martinez, Ricardo Santiago
Larramendy, Marcelo Luis
Di Marzio, Walter Dario
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv DNA DAMAGE
HERBICIDES
INSECTICIDES
SINGLE CELL GELL ELECTROFORESIS ASSAY
COMMERCIAL FORMULATIONS
SUBLETHAL EFFECTS
topic DNA DAMAGE
HERBICIDES
INSECTICIDES
SINGLE CELL GELL ELECTROFORESIS ASSAY
COMMERCIAL FORMULATIONS
SUBLETHAL EFFECTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The organophosphorus herbicide glyphosate (GLY) and the organophosphate insecticide chlorpyriphos (CPF) are key pesticides in modern management cultures worldwide. Sublethal toxicity of the commercial herbicide formulation Roundup® and the insecticide formulation Terfos® were evaluated on Eisenia fetida coelomocytes exposed under in vivo and ex vivo laboratory conditions. Induction of DNA single-strand breaks evaluated by the single cell gel electrophoresis assay and coelomocyte viability as well as alterations in coelomocyte trophic indexes were employed as endpoints for genotoxicity and cytotoxicity, respectively. Specimens were exposed at concentrations corresponding to recommended pesticide field application rate, and endpoints were evaluated after 7 and 14 days of treatment (in vivo exposure). In addition, coelomocytes were exposed to aqueous leachate of pesticide-contaminated soils during 1 h (ex vivo exposure). Earthworms exposed to Roundup® and Terfos® showed an increased frequency of DNA damage. Also, a decrease of coelomocyte viability and decrease of trophic indexes were observed in all treatments. The results demonstrate that either GLY- and CPF-based formulations exerted genotoxic as well as cytotoxic effects in coelomocytes of E. fetida exposed in vivo and ex vivo.
Fil: Curieses, Silvana Patricia. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sáenz, María Elena. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alberdi, José Luis. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina
Fil: Martinez, Ricardo Santiago. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Di Marzio, Walter Dario. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Investigación en Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The organophosphorus herbicide glyphosate (GLY) and the organophosphate insecticide chlorpyriphos (CPF) are key pesticides in modern management cultures worldwide. Sublethal toxicity of the commercial herbicide formulation Roundup® and the insecticide formulation Terfos® were evaluated on Eisenia fetida coelomocytes exposed under in vivo and ex vivo laboratory conditions. Induction of DNA single-strand breaks evaluated by the single cell gel electrophoresis assay and coelomocyte viability as well as alterations in coelomocyte trophic indexes were employed as endpoints for genotoxicity and cytotoxicity, respectively. Specimens were exposed at concentrations corresponding to recommended pesticide field application rate, and endpoints were evaluated after 7 and 14 days of treatment (in vivo exposure). In addition, coelomocytes were exposed to aqueous leachate of pesticide-contaminated soils during 1 h (ex vivo exposure). Earthworms exposed to Roundup® and Terfos® showed an increased frequency of DNA damage. Also, a decrease of coelomocyte viability and decrease of trophic indexes were observed in all treatments. The results demonstrate that either GLY- and CPF-based formulations exerted genotoxic as well as cytotoxic effects in coelomocytes of E. fetida exposed in vivo and ex vivo.
publishDate 2018
dc.date.none.fl_str_mv 2018-07-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/131594
Curieses, Silvana Patricia; Sáenz, María Elena; Alberdi, José Luis; Martinez, Ricardo Santiago; Larramendy, Marcelo Luis; et al.; Genotoxic evidences of glyphosate and chlorpyriphos on eisenia fetida coelomocytes; Scholars.Direct; Advances in Environmental Studies; 2; 2; 6-7-2018; 82-90
2642-4231
CONICET Digital
CONICET
url http://hdl.handle.net/11336/131594
identifier_str_mv Curieses, Silvana Patricia; Sáenz, María Elena; Alberdi, José Luis; Martinez, Ricardo Santiago; Larramendy, Marcelo Luis; et al.; Genotoxic evidences of glyphosate and chlorpyriphos on eisenia fetida coelomocytes; Scholars.Direct; Advances in Environmental Studies; 2; 2; 6-7-2018; 82-90
2642-4231
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.36959/742/206
info:eu-repo/semantics/altIdentifier/url/https://scholars.direct/Articles/environmental-studies/aes-2-011.php?jid=environmental-studies
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scholars.Direct
publisher.none.fl_str_mv Scholars.Direct
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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