Hipocampal hormone receptors expression in late pregnant and lactating rats: effect of mild hyperthyroidism

Autores
Neira, Flavia Judith; Sánchez, María Belén; Pietrobon, Elisa Oliva; Mackern Oberti, Juan Pablo; Soaje, Marta; Jahn, Graciela Alma; Valdez, Susana Ruth
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Thyroid disorders are associated with anxiety, depression and disturb responses to stress. The effect of stress is associated with functional changes in hippocampus (HpC) and hypothalamus by activating the hypothalamichypophysis-adrenal axis (HHA) and glucocorticoid release induced to stress. This response is physiologically attenuated during lactation. We found that mild hyperthyroidism (HyperT) increases stress-induced serum prolactin (PRL) and corticosterone secretion in lactating rats suggesting that HHA remains activated in lactation. To explore possible causes of this effect we studied the expression of thyroid receptors (TR), the long isoform of PRL receptor (PRLRl), members of the PRL signaling pathway, estradiol receptor (ER) and glucocorticoid receptor (GR) in HpC of Wistar female rats in different reproductive states (day 19 of gestation (G19), 2 (L2, early lactation) and 12 (L12) of lactation) in control (Co) and HyperT rats. Mild HyperT was induced with T4 (0.1 mg/kg/day, s.c.), a dose that allows the maintenance of lactation. HpC mRNA was obtained and the expression of receptors TRa1, TRa2, TRb1, TRb2, ERa, GR and PRLRl and members of the PRL signaling pathway (STAT5b, SOCS1 and SOCS3) was determined by RTqPCR. HpC mRNA content of TRs, STAT5b (activator of PRL signaling) and SOCS1 (supressor of PRL signaling) decreased from G19 to L12 in Co and HyperT rats (p<0.05). HyperT induced increases of TRa2 (P< 0.05 vs Co) and TRb2 in L2 (p< 0.01 vs. Co) without changes in others isoforms. HyperT increased STAT5b in G19 (p<0.05 vs. Co). ERa and PRLR mRNAs were unchanged by treatment or reproductive state while GR increased in L12 in both groups (p<0.05 vs. G19).These results indicate a physiological decline in HpC responsiveness to thyroid and PRL hormones in the transition from pregnancy to lactation in Co, while the increases observed in TRa2, TRb2 and STAT5b described in HyperT rats may be involved in the persistence of high HHA axis reactivity.
Fil: Neira, Flavia Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Sánchez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Pietrobon, Elisa Oliva. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Mackern Oberti, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Soaje, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Jahn, Graciela Alma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Valdez, Susana Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimenta; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Nanomedicinas
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Materia
LATE PREGNANCY
LACTATION
HIPPOCAMPAL RECEPTORS
HYPERTHYROIDISM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/211074
Acceder
id CONICETDig_ceb350b82da0966d10b9f18fff45b680
oai_identifier_str oai:ri.conicet.gov.ar:11336/211074
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hipocampal hormone receptors expression in late pregnant and lactating rats: effect of mild hyperthyroidismNeira, Flavia JudithSánchez, María BelénPietrobon, Elisa OlivaMackern Oberti, Juan PabloSoaje, MartaJahn, Graciela AlmaValdez, Susana RuthLATE PREGNANCYLACTATIONHIPPOCAMPAL RECEPTORSHYPERTHYROIDISMhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Thyroid disorders are associated with anxiety, depression and disturb responses to stress. The effect of stress is associated with functional changes in hippocampus (HpC) and hypothalamus by activating the hypothalamichypophysis-adrenal axis (HHA) and glucocorticoid release induced to stress. This response is physiologically attenuated during lactation. We found that mild hyperthyroidism (HyperT) increases stress-induced serum prolactin (PRL) and corticosterone secretion in lactating rats suggesting that HHA remains activated in lactation. To explore possible causes of this effect we studied the expression of thyroid receptors (TR), the long isoform of PRL receptor (PRLRl), members of the PRL signaling pathway, estradiol receptor (ER) and glucocorticoid receptor (GR) in HpC of Wistar female rats in different reproductive states (day 19 of gestation (G19), 2 (L2, early lactation) and 12 (L12) of lactation) in control (Co) and HyperT rats. Mild HyperT was induced with T4 (0.1 mg/kg/day, s.c.), a dose that allows the maintenance of lactation. HpC mRNA was obtained and the expression of receptors TRa1, TRa2, TRb1, TRb2, ERa, GR and PRLRl and members of the PRL signaling pathway (STAT5b, SOCS1 and SOCS3) was determined by RTqPCR. HpC mRNA content of TRs, STAT5b (activator of PRL signaling) and SOCS1 (supressor of PRL signaling) decreased from G19 to L12 in Co and HyperT rats (p<0.05). HyperT induced increases of TRa2 (P< 0.05 vs Co) and TRb2 in L2 (p< 0.01 vs. Co) without changes in others isoforms. HyperT increased STAT5b in G19 (p<0.05 vs. Co). ERa and PRLR mRNAs were unchanged by treatment or reproductive state while GR increased in L12 in both groups (p<0.05 vs. G19).These results indicate a physiological decline in HpC responsiveness to thyroid and PRL hormones in the transition from pregnancy to lactation in Co, while the increases observed in TRa2, TRb2 and STAT5b described in HyperT rats may be involved in the persistence of high HHA axis reactivity.Fil: Neira, Flavia Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Sánchez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Pietrobon, Elisa Oliva. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Mackern Oberti, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Soaje, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Jahn, Graciela Alma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Valdez, Susana Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaLXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimenta; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de NanomedicinasAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista MedicinaCostas, Monica AlejandraMerino, GabrielaAzurmendi, Pablo Javier2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/211074Hipocampal hormone receptors expression in late pregnant and lactating rats: effect of mild hyperthyroidism; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimenta; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 216-2160025-7680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol79-19/s4/vol79_s4.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:13:43Zoai:ri.conicet.gov.ar:11336/211074instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:13:44.12CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hipocampal hormone receptors expression in late pregnant and lactating rats: effect of mild hyperthyroidism
title Hipocampal hormone receptors expression in late pregnant and lactating rats: effect of mild hyperthyroidism
spellingShingle Hipocampal hormone receptors expression in late pregnant and lactating rats: effect of mild hyperthyroidism
Neira, Flavia Judith
LATE PREGNANCY
LACTATION
HIPPOCAMPAL RECEPTORS
HYPERTHYROIDISM
title_short Hipocampal hormone receptors expression in late pregnant and lactating rats: effect of mild hyperthyroidism
title_full Hipocampal hormone receptors expression in late pregnant and lactating rats: effect of mild hyperthyroidism
title_fullStr Hipocampal hormone receptors expression in late pregnant and lactating rats: effect of mild hyperthyroidism
title_full_unstemmed Hipocampal hormone receptors expression in late pregnant and lactating rats: effect of mild hyperthyroidism
title_sort Hipocampal hormone receptors expression in late pregnant and lactating rats: effect of mild hyperthyroidism
dc.creator.none.fl_str_mv Neira, Flavia Judith
Sánchez, María Belén
Pietrobon, Elisa Oliva
Mackern Oberti, Juan Pablo
Soaje, Marta
Jahn, Graciela Alma
Valdez, Susana Ruth
author Neira, Flavia Judith
author_facet Neira, Flavia Judith
Sánchez, María Belén
Pietrobon, Elisa Oliva
Mackern Oberti, Juan Pablo
Soaje, Marta
Jahn, Graciela Alma
Valdez, Susana Ruth
author_role author
author2 Sánchez, María Belén
Pietrobon, Elisa Oliva
Mackern Oberti, Juan Pablo
Soaje, Marta
Jahn, Graciela Alma
Valdez, Susana Ruth
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Costas, Monica Alejandra
Merino, Gabriela
Azurmendi, Pablo Javier
dc.subject.none.fl_str_mv LATE PREGNANCY
LACTATION
HIPPOCAMPAL RECEPTORS
HYPERTHYROIDISM
topic LATE PREGNANCY
LACTATION
HIPPOCAMPAL RECEPTORS
HYPERTHYROIDISM
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Thyroid disorders are associated with anxiety, depression and disturb responses to stress. The effect of stress is associated with functional changes in hippocampus (HpC) and hypothalamus by activating the hypothalamichypophysis-adrenal axis (HHA) and glucocorticoid release induced to stress. This response is physiologically attenuated during lactation. We found that mild hyperthyroidism (HyperT) increases stress-induced serum prolactin (PRL) and corticosterone secretion in lactating rats suggesting that HHA remains activated in lactation. To explore possible causes of this effect we studied the expression of thyroid receptors (TR), the long isoform of PRL receptor (PRLRl), members of the PRL signaling pathway, estradiol receptor (ER) and glucocorticoid receptor (GR) in HpC of Wistar female rats in different reproductive states (day 19 of gestation (G19), 2 (L2, early lactation) and 12 (L12) of lactation) in control (Co) and HyperT rats. Mild HyperT was induced with T4 (0.1 mg/kg/day, s.c.), a dose that allows the maintenance of lactation. HpC mRNA was obtained and the expression of receptors TRa1, TRa2, TRb1, TRb2, ERa, GR and PRLRl and members of the PRL signaling pathway (STAT5b, SOCS1 and SOCS3) was determined by RTqPCR. HpC mRNA content of TRs, STAT5b (activator of PRL signaling) and SOCS1 (supressor of PRL signaling) decreased from G19 to L12 in Co and HyperT rats (p<0.05). HyperT induced increases of TRa2 (P< 0.05 vs Co) and TRb2 in L2 (p< 0.01 vs. Co) without changes in others isoforms. HyperT increased STAT5b in G19 (p<0.05 vs. Co). ERa and PRLR mRNAs were unchanged by treatment or reproductive state while GR increased in L12 in both groups (p<0.05 vs. G19).These results indicate a physiological decline in HpC responsiveness to thyroid and PRL hormones in the transition from pregnancy to lactation in Co, while the increases observed in TRa2, TRb2 and STAT5b described in HyperT rats may be involved in the persistence of high HHA axis reactivity.
Fil: Neira, Flavia Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Sánchez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Pietrobon, Elisa Oliva. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Mackern Oberti, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Soaje, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Jahn, Graciela Alma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Valdez, Susana Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimenta; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Nanomedicinas
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
description Thyroid disorders are associated with anxiety, depression and disturb responses to stress. The effect of stress is associated with functional changes in hippocampus (HpC) and hypothalamus by activating the hypothalamichypophysis-adrenal axis (HHA) and glucocorticoid release induced to stress. This response is physiologically attenuated during lactation. We found that mild hyperthyroidism (HyperT) increases stress-induced serum prolactin (PRL) and corticosterone secretion in lactating rats suggesting that HHA remains activated in lactation. To explore possible causes of this effect we studied the expression of thyroid receptors (TR), the long isoform of PRL receptor (PRLRl), members of the PRL signaling pathway, estradiol receptor (ER) and glucocorticoid receptor (GR) in HpC of Wistar female rats in different reproductive states (day 19 of gestation (G19), 2 (L2, early lactation) and 12 (L12) of lactation) in control (Co) and HyperT rats. Mild HyperT was induced with T4 (0.1 mg/kg/day, s.c.), a dose that allows the maintenance of lactation. HpC mRNA was obtained and the expression of receptors TRa1, TRa2, TRb1, TRb2, ERa, GR and PRLRl and members of the PRL signaling pathway (STAT5b, SOCS1 and SOCS3) was determined by RTqPCR. HpC mRNA content of TRs, STAT5b (activator of PRL signaling) and SOCS1 (supressor of PRL signaling) decreased from G19 to L12 in Co and HyperT rats (p<0.05). HyperT induced increases of TRa2 (P< 0.05 vs Co) and TRb2 in L2 (p< 0.01 vs. Co) without changes in others isoforms. HyperT increased STAT5b in G19 (p<0.05 vs. Co). ERa and PRLR mRNAs were unchanged by treatment or reproductive state while GR increased in L12 in both groups (p<0.05 vs. G19).These results indicate a physiological decline in HpC responsiveness to thyroid and PRL hormones in the transition from pregnancy to lactation in Co, while the increases observed in TRa2, TRb2 and STAT5b described in HyperT rats may be involved in the persistence of high HHA axis reactivity.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/211074
Hipocampal hormone receptors expression in late pregnant and lactating rats: effect of mild hyperthyroidism; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimenta; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 216-216
0025-7680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/211074
identifier_str_mv Hipocampal hormone receptors expression in late pregnant and lactating rats: effect of mild hyperthyroidism; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimenta; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas; VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 216-216
0025-7680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol79-19/s4/vol79_s4.pdf
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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