Study of peptide–ligand interactions in open-tubular capillary columns covalently modified with porphyrins

Autores
Yone, Angel; Carballo, Romina Raquel; Grela, Denise Agata; Rezzano, Irene Noemí; Vizioli, Nora Matilde
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The inner surface of fused silica capillaries has been covalently modified with different porphyrins (deuteroporphyrin, complexes of deuteroporphyrin with metal ions Fe(III), Cu(II), Zn(II), Ni(II), and Cu(II)–meso–tetra (carboxyphenyl) porphyrin) and it was applied for the separation of biologically active peptides by open-tubular capillary electrochromatography. Separations were performed in a mobile phase composed of 25 mM potassium phosphate, pH 4.0, 5% v/v ACN and 10 mM hydroquinone. Changes in the effective electrophoretic mobility of peptides were studied concerning porphyrin central metal atom, attachment geometry, and the presence of coordinating or aromatic amino acid residues in the peptide sequence. The results showed that differences in metal core on the porphyrin and the spatial conformation of attached porphyrin result in changes in the analyte interaction with the stationary phase.
Fil: Yone, Angel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carballo, Romina Raquel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grela, Denise Agata. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina
Fil: Rezzano, Irene Noemí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vizioli, Nora Matilde. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Open Tubular Capillary Electrochromatography
Peptides
Porphyrins
Separation Techniques
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18204
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/18204
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Study of peptide–ligand interactions in open-tubular capillary columns covalently modified with porphyrinsYone, AngelCarballo, Romina RaquelGrela, Denise AgataRezzano, Irene NoemíVizioli, Nora MatildeOpen Tubular Capillary ElectrochromatographyPeptidesPorphyrinsSeparation Techniqueshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The inner surface of fused silica capillaries has been covalently modified with different porphyrins (deuteroporphyrin, complexes of deuteroporphyrin with metal ions Fe(III), Cu(II), Zn(II), Ni(II), and Cu(II)–meso–tetra (carboxyphenyl) porphyrin) and it was applied for the separation of biologically active peptides by open-tubular capillary electrochromatography. Separations were performed in a mobile phase composed of 25 mM potassium phosphate, pH 4.0, 5% v/v ACN and 10 mM hydroquinone. Changes in the effective electrophoretic mobility of peptides were studied concerning porphyrin central metal atom, attachment geometry, and the presence of coordinating or aromatic amino acid residues in the peptide sequence. The results showed that differences in metal core on the porphyrin and the spatial conformation of attached porphyrin result in changes in the analyte interaction with the stationary phase.Fil: Yone, Angel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carballo, Romina Raquel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Grela, Denise Agata. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; ArgentinaFil: Rezzano, Irene Noemí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vizioli, Nora Matilde. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley VCH Verlag2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18204Yone, Angel; Carballo, Romina Raquel; Grela, Denise Agata; Rezzano, Irene Noemí; Vizioli, Nora Matilde; Study of peptide–ligand interactions in open-tubular capillary columns covalently modified with porphyrins; Wiley VCH Verlag; Electrophoresis; 32; 20; 10-2011; 2840-28470173-08351522-2683CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.201100087/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201100087info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:35:12Zoai:ri.conicet.gov.ar:11336/18204instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:35:12.566CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Study of peptide–ligand interactions in open-tubular capillary columns covalently modified with porphyrins
title Study of peptide–ligand interactions in open-tubular capillary columns covalently modified with porphyrins
spellingShingle Study of peptide–ligand interactions in open-tubular capillary columns covalently modified with porphyrins
Yone, Angel
Open Tubular Capillary Electrochromatography
Peptides
Porphyrins
Separation Techniques
title_short Study of peptide–ligand interactions in open-tubular capillary columns covalently modified with porphyrins
title_full Study of peptide–ligand interactions in open-tubular capillary columns covalently modified with porphyrins
title_fullStr Study of peptide–ligand interactions in open-tubular capillary columns covalently modified with porphyrins
title_full_unstemmed Study of peptide–ligand interactions in open-tubular capillary columns covalently modified with porphyrins
title_sort Study of peptide–ligand interactions in open-tubular capillary columns covalently modified with porphyrins
dc.creator.none.fl_str_mv Yone, Angel
Carballo, Romina Raquel
Grela, Denise Agata
Rezzano, Irene Noemí
Vizioli, Nora Matilde
author Yone, Angel
author_facet Yone, Angel
Carballo, Romina Raquel
Grela, Denise Agata
Rezzano, Irene Noemí
Vizioli, Nora Matilde
author_role author
author2 Carballo, Romina Raquel
Grela, Denise Agata
Rezzano, Irene Noemí
Vizioli, Nora Matilde
author2_role author
author
author
author
dc.subject.none.fl_str_mv Open Tubular Capillary Electrochromatography
Peptides
Porphyrins
Separation Techniques
topic Open Tubular Capillary Electrochromatography
Peptides
Porphyrins
Separation Techniques
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The inner surface of fused silica capillaries has been covalently modified with different porphyrins (deuteroporphyrin, complexes of deuteroporphyrin with metal ions Fe(III), Cu(II), Zn(II), Ni(II), and Cu(II)–meso–tetra (carboxyphenyl) porphyrin) and it was applied for the separation of biologically active peptides by open-tubular capillary electrochromatography. Separations were performed in a mobile phase composed of 25 mM potassium phosphate, pH 4.0, 5% v/v ACN and 10 mM hydroquinone. Changes in the effective electrophoretic mobility of peptides were studied concerning porphyrin central metal atom, attachment geometry, and the presence of coordinating or aromatic amino acid residues in the peptide sequence. The results showed that differences in metal core on the porphyrin and the spatial conformation of attached porphyrin result in changes in the analyte interaction with the stationary phase.
Fil: Yone, Angel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carballo, Romina Raquel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grela, Denise Agata. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina
Fil: Rezzano, Irene Noemí. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vizioli, Nora Matilde. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The inner surface of fused silica capillaries has been covalently modified with different porphyrins (deuteroporphyrin, complexes of deuteroporphyrin with metal ions Fe(III), Cu(II), Zn(II), Ni(II), and Cu(II)–meso–tetra (carboxyphenyl) porphyrin) and it was applied for the separation of biologically active peptides by open-tubular capillary electrochromatography. Separations were performed in a mobile phase composed of 25 mM potassium phosphate, pH 4.0, 5% v/v ACN and 10 mM hydroquinone. Changes in the effective electrophoretic mobility of peptides were studied concerning porphyrin central metal atom, attachment geometry, and the presence of coordinating or aromatic amino acid residues in the peptide sequence. The results showed that differences in metal core on the porphyrin and the spatial conformation of attached porphyrin result in changes in the analyte interaction with the stationary phase.
publishDate 2011
dc.date.none.fl_str_mv 2011-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18204
Yone, Angel; Carballo, Romina Raquel; Grela, Denise Agata; Rezzano, Irene Noemí; Vizioli, Nora Matilde; Study of peptide–ligand interactions in open-tubular capillary columns covalently modified with porphyrins; Wiley VCH Verlag; Electrophoresis; 32; 20; 10-2011; 2840-2847
0173-0835
1522-2683
CONICET Digital
CONICET
url http://hdl.handle.net/11336/18204
identifier_str_mv Yone, Angel; Carballo, Romina Raquel; Grela, Denise Agata; Rezzano, Irene Noemí; Vizioli, Nora Matilde; Study of peptide–ligand interactions in open-tubular capillary columns covalently modified with porphyrins; Wiley VCH Verlag; Electrophoresis; 32; 20; 10-2011; 2840-2847
0173-0835
1522-2683
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.201100087/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/elps.201100087
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083478717726720
score 13.22299

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