Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells

Autores
Wu, Ling; Bluguermann, Carolina; Kyupelyan, Levon; Latour, Brooke; Gonzalez, Stephanie; Shah, Saumya; Galic, Zoran; Ge, Sundi; Zhu, Yuhua; Petrigliano, Frank A.; Nsair, Ali; Miriuka, Santiago Gabriel; Li, Xinmin; Lyons, Karen M.; Crooks, Gay M.; McAllister, David R.; Van Handel, Ben; Adams, John S.; Evseenko, Denis
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult stem/progenitor cells have been unsuccessful. We hypothesized that recapitulation of the human developmental chondrogenic program using pluripotent stem cells (PSCs) may represent a superior approach for cartilage restoration. Using laser-capture microdissection followed by microarray analysis, we first defined a surface phenotype (CD166(low/neg)CD146(low/neg)CD73(+)CD44(low)BMPR1B(+)) distinguishing the earliest cartilage committed cells (prechondrocytes) at 5-6 weeks of development. Functional studies confirmed these cells are chondrocyte progenitors. From 12 weeks, only the superficial layers of articular cartilage were enriched in cells with this progenitor phenotype. Isolation of cells with a similar immunophenotype from differentiating human PSCs revealed a population of CD166(low/neg)BMPR1B(+) putative cartilage-committed progenitors. Taken as a whole, these data define a developmental approach for the generation of highly purified functional human chondrocytes from PSCs that could enable substantial progress in cartilage tissue engineering.
Fil: Wu, Ling. University of California at Los Angeles; Estados Unidos
Fil: Bluguermann, Carolina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of California at Los Angeles; Estados Unidos
Fil: Kyupelyan, Levon. University of California at Los Angeles; Estados Unidos
Fil: Latour, Brooke. University of California at Los Angeles; Estados Unidos
Fil: Gonzalez, Stephanie. University of California at Los Angeles; Estados Unidos
Fil: Shah, Saumya. University of California at Los Angeles; Estados Unidos
Fil: Galic, Zoran. University of California at Los Angeles; Estados Unidos
Fil: Ge, Sundi. University of California at Los Angeles; Estados Unidos
Fil: Zhu, Yuhua. University of California at Los Angeles; Estados Unidos
Fil: Petrigliano, Frank A.. University of California at Los Angeles; Estados Unidos
Fil: Nsair, Ali. University of California at Los Angeles; Estados Unidos
Fil: Miriuka, Santiago Gabriel. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Li, Xinmin. University of California at Los Angeles; Estados Unidos
Fil: Lyons, Karen M.. University of California at Los Angeles; Estados Unidos
Fil: Crooks, Gay M.. University of California at Los Angeles; Estados Unidos
Fil: McAllister, David R.. University of California at Los Angeles; Estados Unidos
Fil: Van Handel, Ben. Novogenix Laboratories; Estados Unidos
Fil: Adams, John S.. University of California at Los Angeles; Estados Unidos
Fil: Evseenko, Denis. University of California at Los Angeles; Estados Unidos
Materia
CHONDROGENESIS
HUMAN
PLURIPOTENT
DEVELOPMENT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29031

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network_name_str CONICET Digital (CONICET)
spelling Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem CellsWu, LingBluguermann, CarolinaKyupelyan, LevonLatour, BrookeGonzalez, StephanieShah, SaumyaGalic, ZoranGe, SundiZhu, YuhuaPetrigliano, Frank A.Nsair, AliMiriuka, Santiago GabrielLi, XinminLyons, Karen M.Crooks, Gay M.McAllister, David R.Van Handel, BenAdams, John S.Evseenko, DenisCHONDROGENESISHUMANPLURIPOTENTDEVELOPMENThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult stem/progenitor cells have been unsuccessful. We hypothesized that recapitulation of the human developmental chondrogenic program using pluripotent stem cells (PSCs) may represent a superior approach for cartilage restoration. Using laser-capture microdissection followed by microarray analysis, we first defined a surface phenotype (CD166(low/neg)CD146(low/neg)CD73(+)CD44(low)BMPR1B(+)) distinguishing the earliest cartilage committed cells (prechondrocytes) at 5-6 weeks of development. Functional studies confirmed these cells are chondrocyte progenitors. From 12 weeks, only the superficial layers of articular cartilage were enriched in cells with this progenitor phenotype. Isolation of cells with a similar immunophenotype from differentiating human PSCs revealed a population of CD166(low/neg)BMPR1B(+) putative cartilage-committed progenitors. Taken as a whole, these data define a developmental approach for the generation of highly purified functional human chondrocytes from PSCs that could enable substantial progress in cartilage tissue engineering.Fil: Wu, Ling. University of California at Los Angeles; Estados UnidosFil: Bluguermann, Carolina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of California at Los Angeles; Estados UnidosFil: Kyupelyan, Levon. University of California at Los Angeles; Estados UnidosFil: Latour, Brooke. University of California at Los Angeles; Estados UnidosFil: Gonzalez, Stephanie. University of California at Los Angeles; Estados UnidosFil: Shah, Saumya. University of California at Los Angeles; Estados UnidosFil: Galic, Zoran. University of California at Los Angeles; Estados UnidosFil: Ge, Sundi. University of California at Los Angeles; Estados UnidosFil: Zhu, Yuhua. University of California at Los Angeles; Estados UnidosFil: Petrigliano, Frank A.. University of California at Los Angeles; Estados UnidosFil: Nsair, Ali. University of California at Los Angeles; Estados UnidosFil: Miriuka, Santiago Gabriel. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Li, Xinmin. University of California at Los Angeles; Estados UnidosFil: Lyons, Karen M.. University of California at Los Angeles; Estados UnidosFil: Crooks, Gay M.. University of California at Los Angeles; Estados UnidosFil: McAllister, David R.. University of California at Los Angeles; Estados UnidosFil: Van Handel, Ben. Novogenix Laboratories; Estados UnidosFil: Adams, John S.. University of California at Los Angeles; Estados UnidosFil: Evseenko, Denis. University of California at Los Angeles; Estados UnidosCell Press2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29031Wu, Ling; Bluguermann, Carolina; Kyupelyan, Levon; Latour, Brooke; Gonzalez, Stephanie; et al.; Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells; Cell Press; Stem Cell Reports; 1; 6; 12-2013; 575-5892213-6711CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871393/info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2213671113001240info:eu-repo/semantics/altIdentifier/doi/10.1016/j.stemcr.2013.10.012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:13Zoai:ri.conicet.gov.ar:11336/29031instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:14.062CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells
title Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells
spellingShingle Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells
Wu, Ling
CHONDROGENESIS
HUMAN
PLURIPOTENT
DEVELOPMENT
title_short Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells
title_full Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells
title_fullStr Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells
title_full_unstemmed Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells
title_sort Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells
dc.creator.none.fl_str_mv Wu, Ling
Bluguermann, Carolina
Kyupelyan, Levon
Latour, Brooke
Gonzalez, Stephanie
Shah, Saumya
Galic, Zoran
Ge, Sundi
Zhu, Yuhua
Petrigliano, Frank A.
Nsair, Ali
Miriuka, Santiago Gabriel
Li, Xinmin
Lyons, Karen M.
Crooks, Gay M.
McAllister, David R.
Van Handel, Ben
Adams, John S.
Evseenko, Denis
author Wu, Ling
author_facet Wu, Ling
Bluguermann, Carolina
Kyupelyan, Levon
Latour, Brooke
Gonzalez, Stephanie
Shah, Saumya
Galic, Zoran
Ge, Sundi
Zhu, Yuhua
Petrigliano, Frank A.
Nsair, Ali
Miriuka, Santiago Gabriel
Li, Xinmin
Lyons, Karen M.
Crooks, Gay M.
McAllister, David R.
Van Handel, Ben
Adams, John S.
Evseenko, Denis
author_role author
author2 Bluguermann, Carolina
Kyupelyan, Levon
Latour, Brooke
Gonzalez, Stephanie
Shah, Saumya
Galic, Zoran
Ge, Sundi
Zhu, Yuhua
Petrigliano, Frank A.
Nsair, Ali
Miriuka, Santiago Gabriel
Li, Xinmin
Lyons, Karen M.
Crooks, Gay M.
McAllister, David R.
Van Handel, Ben
Adams, John S.
Evseenko, Denis
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CHONDROGENESIS
HUMAN
PLURIPOTENT
DEVELOPMENT
topic CHONDROGENESIS
HUMAN
PLURIPOTENT
DEVELOPMENT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult stem/progenitor cells have been unsuccessful. We hypothesized that recapitulation of the human developmental chondrogenic program using pluripotent stem cells (PSCs) may represent a superior approach for cartilage restoration. Using laser-capture microdissection followed by microarray analysis, we first defined a surface phenotype (CD166(low/neg)CD146(low/neg)CD73(+)CD44(low)BMPR1B(+)) distinguishing the earliest cartilage committed cells (prechondrocytes) at 5-6 weeks of development. Functional studies confirmed these cells are chondrocyte progenitors. From 12 weeks, only the superficial layers of articular cartilage were enriched in cells with this progenitor phenotype. Isolation of cells with a similar immunophenotype from differentiating human PSCs revealed a population of CD166(low/neg)BMPR1B(+) putative cartilage-committed progenitors. Taken as a whole, these data define a developmental approach for the generation of highly purified functional human chondrocytes from PSCs that could enable substantial progress in cartilage tissue engineering.
Fil: Wu, Ling. University of California at Los Angeles; Estados Unidos
Fil: Bluguermann, Carolina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of California at Los Angeles; Estados Unidos
Fil: Kyupelyan, Levon. University of California at Los Angeles; Estados Unidos
Fil: Latour, Brooke. University of California at Los Angeles; Estados Unidos
Fil: Gonzalez, Stephanie. University of California at Los Angeles; Estados Unidos
Fil: Shah, Saumya. University of California at Los Angeles; Estados Unidos
Fil: Galic, Zoran. University of California at Los Angeles; Estados Unidos
Fil: Ge, Sundi. University of California at Los Angeles; Estados Unidos
Fil: Zhu, Yuhua. University of California at Los Angeles; Estados Unidos
Fil: Petrigliano, Frank A.. University of California at Los Angeles; Estados Unidos
Fil: Nsair, Ali. University of California at Los Angeles; Estados Unidos
Fil: Miriuka, Santiago Gabriel. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Li, Xinmin. University of California at Los Angeles; Estados Unidos
Fil: Lyons, Karen M.. University of California at Los Angeles; Estados Unidos
Fil: Crooks, Gay M.. University of California at Los Angeles; Estados Unidos
Fil: McAllister, David R.. University of California at Los Angeles; Estados Unidos
Fil: Van Handel, Ben. Novogenix Laboratories; Estados Unidos
Fil: Adams, John S.. University of California at Los Angeles; Estados Unidos
Fil: Evseenko, Denis. University of California at Los Angeles; Estados Unidos
description Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult stem/progenitor cells have been unsuccessful. We hypothesized that recapitulation of the human developmental chondrogenic program using pluripotent stem cells (PSCs) may represent a superior approach for cartilage restoration. Using laser-capture microdissection followed by microarray analysis, we first defined a surface phenotype (CD166(low/neg)CD146(low/neg)CD73(+)CD44(low)BMPR1B(+)) distinguishing the earliest cartilage committed cells (prechondrocytes) at 5-6 weeks of development. Functional studies confirmed these cells are chondrocyte progenitors. From 12 weeks, only the superficial layers of articular cartilage were enriched in cells with this progenitor phenotype. Isolation of cells with a similar immunophenotype from differentiating human PSCs revealed a population of CD166(low/neg)BMPR1B(+) putative cartilage-committed progenitors. Taken as a whole, these data define a developmental approach for the generation of highly purified functional human chondrocytes from PSCs that could enable substantial progress in cartilage tissue engineering.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29031
Wu, Ling; Bluguermann, Carolina; Kyupelyan, Levon; Latour, Brooke; Gonzalez, Stephanie; et al.; Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells; Cell Press; Stem Cell Reports; 1; 6; 12-2013; 575-589
2213-6711
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29031
identifier_str_mv Wu, Ling; Bluguermann, Carolina; Kyupelyan, Levon; Latour, Brooke; Gonzalez, Stephanie; et al.; Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells; Cell Press; Stem Cell Reports; 1; 6; 12-2013; 575-589
2213-6711
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871393/
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2213671113001240
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.stemcr.2013.10.012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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