Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells
- Autores
- Wu, Ling; Bluguermann, Carolina; Kyupelyan, Levon; Latour, Brooke; Gonzalez, Stephanie; Shah, Saumya; Galic, Zoran; Ge, Sundi; Zhu, Yuhua; Petrigliano, Frank A.; Nsair, Ali; Miriuka, Santiago Gabriel; Li, Xinmin; Lyons, Karen M.; Crooks, Gay M.; McAllister, David R.; Van Handel, Ben; Adams, John S.; Evseenko, Denis
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult stem/progenitor cells have been unsuccessful. We hypothesized that recapitulation of the human developmental chondrogenic program using pluripotent stem cells (PSCs) may represent a superior approach for cartilage restoration. Using laser-capture microdissection followed by microarray analysis, we first defined a surface phenotype (CD166(low/neg)CD146(low/neg)CD73(+)CD44(low)BMPR1B(+)) distinguishing the earliest cartilage committed cells (prechondrocytes) at 5-6 weeks of development. Functional studies confirmed these cells are chondrocyte progenitors. From 12 weeks, only the superficial layers of articular cartilage were enriched in cells with this progenitor phenotype. Isolation of cells with a similar immunophenotype from differentiating human PSCs revealed a population of CD166(low/neg)BMPR1B(+) putative cartilage-committed progenitors. Taken as a whole, these data define a developmental approach for the generation of highly purified functional human chondrocytes from PSCs that could enable substantial progress in cartilage tissue engineering.
Fil: Wu, Ling. University of California at Los Angeles; Estados Unidos
Fil: Bluguermann, Carolina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of California at Los Angeles; Estados Unidos
Fil: Kyupelyan, Levon. University of California at Los Angeles; Estados Unidos
Fil: Latour, Brooke. University of California at Los Angeles; Estados Unidos
Fil: Gonzalez, Stephanie. University of California at Los Angeles; Estados Unidos
Fil: Shah, Saumya. University of California at Los Angeles; Estados Unidos
Fil: Galic, Zoran. University of California at Los Angeles; Estados Unidos
Fil: Ge, Sundi. University of California at Los Angeles; Estados Unidos
Fil: Zhu, Yuhua. University of California at Los Angeles; Estados Unidos
Fil: Petrigliano, Frank A.. University of California at Los Angeles; Estados Unidos
Fil: Nsair, Ali. University of California at Los Angeles; Estados Unidos
Fil: Miriuka, Santiago Gabriel. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Li, Xinmin. University of California at Los Angeles; Estados Unidos
Fil: Lyons, Karen M.. University of California at Los Angeles; Estados Unidos
Fil: Crooks, Gay M.. University of California at Los Angeles; Estados Unidos
Fil: McAllister, David R.. University of California at Los Angeles; Estados Unidos
Fil: Van Handel, Ben. Novogenix Laboratories; Estados Unidos
Fil: Adams, John S.. University of California at Los Angeles; Estados Unidos
Fil: Evseenko, Denis. University of California at Los Angeles; Estados Unidos - Materia
-
CHONDROGENESIS
HUMAN
PLURIPOTENT
DEVELOPMENT - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/29031
Ver los metadatos del registro completo
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Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem CellsWu, LingBluguermann, CarolinaKyupelyan, LevonLatour, BrookeGonzalez, StephanieShah, SaumyaGalic, ZoranGe, SundiZhu, YuhuaPetrigliano, Frank A.Nsair, AliMiriuka, Santiago GabrielLi, XinminLyons, Karen M.Crooks, Gay M.McAllister, David R.Van Handel, BenAdams, John S.Evseenko, DenisCHONDROGENESISHUMANPLURIPOTENTDEVELOPMENThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult stem/progenitor cells have been unsuccessful. We hypothesized that recapitulation of the human developmental chondrogenic program using pluripotent stem cells (PSCs) may represent a superior approach for cartilage restoration. Using laser-capture microdissection followed by microarray analysis, we first defined a surface phenotype (CD166(low/neg)CD146(low/neg)CD73(+)CD44(low)BMPR1B(+)) distinguishing the earliest cartilage committed cells (prechondrocytes) at 5-6 weeks of development. Functional studies confirmed these cells are chondrocyte progenitors. From 12 weeks, only the superficial layers of articular cartilage were enriched in cells with this progenitor phenotype. Isolation of cells with a similar immunophenotype from differentiating human PSCs revealed a population of CD166(low/neg)BMPR1B(+) putative cartilage-committed progenitors. Taken as a whole, these data define a developmental approach for the generation of highly purified functional human chondrocytes from PSCs that could enable substantial progress in cartilage tissue engineering.Fil: Wu, Ling. University of California at Los Angeles; Estados UnidosFil: Bluguermann, Carolina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of California at Los Angeles; Estados UnidosFil: Kyupelyan, Levon. University of California at Los Angeles; Estados UnidosFil: Latour, Brooke. University of California at Los Angeles; Estados UnidosFil: Gonzalez, Stephanie. University of California at Los Angeles; Estados UnidosFil: Shah, Saumya. University of California at Los Angeles; Estados UnidosFil: Galic, Zoran. University of California at Los Angeles; Estados UnidosFil: Ge, Sundi. University of California at Los Angeles; Estados UnidosFil: Zhu, Yuhua. University of California at Los Angeles; Estados UnidosFil: Petrigliano, Frank A.. University of California at Los Angeles; Estados UnidosFil: Nsair, Ali. University of California at Los Angeles; Estados UnidosFil: Miriuka, Santiago Gabriel. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Li, Xinmin. University of California at Los Angeles; Estados UnidosFil: Lyons, Karen M.. University of California at Los Angeles; Estados UnidosFil: Crooks, Gay M.. University of California at Los Angeles; Estados UnidosFil: McAllister, David R.. University of California at Los Angeles; Estados UnidosFil: Van Handel, Ben. Novogenix Laboratories; Estados UnidosFil: Adams, John S.. University of California at Los Angeles; Estados UnidosFil: Evseenko, Denis. University of California at Los Angeles; Estados UnidosCell Press2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29031Wu, Ling; Bluguermann, Carolina; Kyupelyan, Levon; Latour, Brooke; Gonzalez, Stephanie; et al.; Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells; Cell Press; Stem Cell Reports; 1; 6; 12-2013; 575-5892213-6711CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871393/info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2213671113001240info:eu-repo/semantics/altIdentifier/doi/10.1016/j.stemcr.2013.10.012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:13Zoai:ri.conicet.gov.ar:11336/29031instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:14.062CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells |
title |
Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells |
spellingShingle |
Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells Wu, Ling CHONDROGENESIS HUMAN PLURIPOTENT DEVELOPMENT |
title_short |
Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells |
title_full |
Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells |
title_fullStr |
Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells |
title_full_unstemmed |
Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells |
title_sort |
Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells |
dc.creator.none.fl_str_mv |
Wu, Ling Bluguermann, Carolina Kyupelyan, Levon Latour, Brooke Gonzalez, Stephanie Shah, Saumya Galic, Zoran Ge, Sundi Zhu, Yuhua Petrigliano, Frank A. Nsair, Ali Miriuka, Santiago Gabriel Li, Xinmin Lyons, Karen M. Crooks, Gay M. McAllister, David R. Van Handel, Ben Adams, John S. Evseenko, Denis |
author |
Wu, Ling |
author_facet |
Wu, Ling Bluguermann, Carolina Kyupelyan, Levon Latour, Brooke Gonzalez, Stephanie Shah, Saumya Galic, Zoran Ge, Sundi Zhu, Yuhua Petrigliano, Frank A. Nsair, Ali Miriuka, Santiago Gabriel Li, Xinmin Lyons, Karen M. Crooks, Gay M. McAllister, David R. Van Handel, Ben Adams, John S. Evseenko, Denis |
author_role |
author |
author2 |
Bluguermann, Carolina Kyupelyan, Levon Latour, Brooke Gonzalez, Stephanie Shah, Saumya Galic, Zoran Ge, Sundi Zhu, Yuhua Petrigliano, Frank A. Nsair, Ali Miriuka, Santiago Gabriel Li, Xinmin Lyons, Karen M. Crooks, Gay M. McAllister, David R. Van Handel, Ben Adams, John S. Evseenko, Denis |
author2_role |
author author author author author author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
CHONDROGENESIS HUMAN PLURIPOTENT DEVELOPMENT |
topic |
CHONDROGENESIS HUMAN PLURIPOTENT DEVELOPMENT |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult stem/progenitor cells have been unsuccessful. We hypothesized that recapitulation of the human developmental chondrogenic program using pluripotent stem cells (PSCs) may represent a superior approach for cartilage restoration. Using laser-capture microdissection followed by microarray analysis, we first defined a surface phenotype (CD166(low/neg)CD146(low/neg)CD73(+)CD44(low)BMPR1B(+)) distinguishing the earliest cartilage committed cells (prechondrocytes) at 5-6 weeks of development. Functional studies confirmed these cells are chondrocyte progenitors. From 12 weeks, only the superficial layers of articular cartilage were enriched in cells with this progenitor phenotype. Isolation of cells with a similar immunophenotype from differentiating human PSCs revealed a population of CD166(low/neg)BMPR1B(+) putative cartilage-committed progenitors. Taken as a whole, these data define a developmental approach for the generation of highly purified functional human chondrocytes from PSCs that could enable substantial progress in cartilage tissue engineering. Fil: Wu, Ling. University of California at Los Angeles; Estados Unidos Fil: Bluguermann, Carolina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of California at Los Angeles; Estados Unidos Fil: Kyupelyan, Levon. University of California at Los Angeles; Estados Unidos Fil: Latour, Brooke. University of California at Los Angeles; Estados Unidos Fil: Gonzalez, Stephanie. University of California at Los Angeles; Estados Unidos Fil: Shah, Saumya. University of California at Los Angeles; Estados Unidos Fil: Galic, Zoran. University of California at Los Angeles; Estados Unidos Fil: Ge, Sundi. University of California at Los Angeles; Estados Unidos Fil: Zhu, Yuhua. University of California at Los Angeles; Estados Unidos Fil: Petrigliano, Frank A.. University of California at Los Angeles; Estados Unidos Fil: Nsair, Ali. University of California at Los Angeles; Estados Unidos Fil: Miriuka, Santiago Gabriel. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia. Laboratorio de Biología del Desarrollo Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Li, Xinmin. University of California at Los Angeles; Estados Unidos Fil: Lyons, Karen M.. University of California at Los Angeles; Estados Unidos Fil: Crooks, Gay M.. University of California at Los Angeles; Estados Unidos Fil: McAllister, David R.. University of California at Los Angeles; Estados Unidos Fil: Van Handel, Ben. Novogenix Laboratories; Estados Unidos Fil: Adams, John S.. University of California at Los Angeles; Estados Unidos Fil: Evseenko, Denis. University of California at Los Angeles; Estados Unidos |
description |
Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult stem/progenitor cells have been unsuccessful. We hypothesized that recapitulation of the human developmental chondrogenic program using pluripotent stem cells (PSCs) may represent a superior approach for cartilage restoration. Using laser-capture microdissection followed by microarray analysis, we first defined a surface phenotype (CD166(low/neg)CD146(low/neg)CD73(+)CD44(low)BMPR1B(+)) distinguishing the earliest cartilage committed cells (prechondrocytes) at 5-6 weeks of development. Functional studies confirmed these cells are chondrocyte progenitors. From 12 weeks, only the superficial layers of articular cartilage were enriched in cells with this progenitor phenotype. Isolation of cells with a similar immunophenotype from differentiating human PSCs revealed a population of CD166(low/neg)BMPR1B(+) putative cartilage-committed progenitors. Taken as a whole, these data define a developmental approach for the generation of highly purified functional human chondrocytes from PSCs that could enable substantial progress in cartilage tissue engineering. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/29031 Wu, Ling; Bluguermann, Carolina; Kyupelyan, Levon; Latour, Brooke; Gonzalez, Stephanie; et al.; Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells; Cell Press; Stem Cell Reports; 1; 6; 12-2013; 575-589 2213-6711 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/29031 |
identifier_str_mv |
Wu, Ling; Bluguermann, Carolina; Kyupelyan, Levon; Latour, Brooke; Gonzalez, Stephanie; et al.; Human Developmental Chondrogenesis as a Basis for Engineering Chondrocytes from Pluripotent Stem Cells; Cell Press; Stem Cell Reports; 1; 6; 12-2013; 575-589 2213-6711 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3871393/ info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2213671113001240 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.stemcr.2013.10.012 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Cell Press |
publisher.none.fl_str_mv |
Cell Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |