Bile acids content in brain of common duct ligated rats

Autores
Tripodi, Valeria Paula; Contin, Mario Daniel; Fernández, María AlejandrA; Lemberg, Abraham
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction. Cholestasis leads to liver cell death, fibrosis, cirrhosis, and eventually liver failure. Bile duct ligated rats constitute an interesting model to study the mechanism of cholestasis, and its action on seve-ral organs and tissues, including the brain. Aim. To analyze brain bile acids individually in ligated rats to evaluate if its profile is altered towards a more toxic condition in cholestasis. Material and methods. Male Wistar rats were used and separated in two groups: bile duct ligated rats and sham operated rats (n = 5 in each group). Bile acid profile was assessed in brain homogenates. Alanine aminotransferase, aspartate ami-notransferase, alkaline phosphatase determinations, bilirubin and ammonia plasma concentration were also measured in both groups. Results. Although the total amount of bile acids in control animal brains showed a higher concentration than in bile duct ligated rats, the bile acid profile in this group was found more toxic composition than in controls. Lithocholic acid was present in brain in higher concentration (87.4% of total brain bile acids) in ligated rats and absent in controls. Alkaline phosphatase, bilirubin and ammonia were significantly higher in bile duct ligated rats than in control group. Conclusion. It was found a toxic brain bile acid profile during hepatic cholestasis which could be one of the explanations of hepatic ence-phalopathy observed in cholestatic diseases.
Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Contin, Mario Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Fernández, María AlejandrA. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiopatología; Argentina
Fil: Lemberg, Abraham. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiopatología; Argentina
Materia
BRAIN BILE ACID PROFILE
CHOLESTASIS
LITHOCHOLIC ACID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/197620
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/197620
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bile acids content in brain of common duct ligated ratsTripodi, Valeria PaulaContin, Mario DanielFernández, María AlejandrALemberg, AbrahamBRAIN BILE ACID PROFILECHOLESTASISLITHOCHOLIC ACIDhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Introduction. Cholestasis leads to liver cell death, fibrosis, cirrhosis, and eventually liver failure. Bile duct ligated rats constitute an interesting model to study the mechanism of cholestasis, and its action on seve-ral organs and tissues, including the brain. Aim. To analyze brain bile acids individually in ligated rats to evaluate if its profile is altered towards a more toxic condition in cholestasis. Material and methods. Male Wistar rats were used and separated in two groups: bile duct ligated rats and sham operated rats (n = 5 in each group). Bile acid profile was assessed in brain homogenates. Alanine aminotransferase, aspartate ami-notransferase, alkaline phosphatase determinations, bilirubin and ammonia plasma concentration were also measured in both groups. Results. Although the total amount of bile acids in control animal brains showed a higher concentration than in bile duct ligated rats, the bile acid profile in this group was found more toxic composition than in controls. Lithocholic acid was present in brain in higher concentration (87.4% of total brain bile acids) in ligated rats and absent in controls. Alkaline phosphatase, bilirubin and ammonia were significantly higher in bile duct ligated rats than in control group. Conclusion. It was found a toxic brain bile acid profile during hepatic cholestasis which could be one of the explanations of hepatic ence-phalopathy observed in cholestatic diseases.Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Contin, Mario Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Fernández, María AlejandrA. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiopatología; ArgentinaFil: Lemberg, Abraham. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiopatología; ArgentinaMexican Association of Hepatology2012-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/197620Tripodi, Valeria Paula; Contin, Mario Daniel; Fernández, María AlejandrA; Lemberg, Abraham; Bile acids content in brain of common duct ligated rats; Mexican Association of Hepatology; Annals of Hepatology; 11; 6; 11-2012; 930-9341665-2681CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/S1665-2681(19)31420-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:47Zoai:ri.conicet.gov.ar:11336/197620instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:47.34CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bile acids content in brain of common duct ligated rats
title Bile acids content in brain of common duct ligated rats
spellingShingle Bile acids content in brain of common duct ligated rats
Tripodi, Valeria Paula
BRAIN BILE ACID PROFILE
CHOLESTASIS
LITHOCHOLIC ACID
title_short Bile acids content in brain of common duct ligated rats
title_full Bile acids content in brain of common duct ligated rats
title_fullStr Bile acids content in brain of common duct ligated rats
title_full_unstemmed Bile acids content in brain of common duct ligated rats
title_sort Bile acids content in brain of common duct ligated rats
dc.creator.none.fl_str_mv Tripodi, Valeria Paula
Contin, Mario Daniel
Fernández, María AlejandrA
Lemberg, Abraham
author Tripodi, Valeria Paula
author_facet Tripodi, Valeria Paula
Contin, Mario Daniel
Fernández, María AlejandrA
Lemberg, Abraham
author_role author
author2 Contin, Mario Daniel
Fernández, María AlejandrA
Lemberg, Abraham
author2_role author
author
author
dc.subject.none.fl_str_mv BRAIN BILE ACID PROFILE
CHOLESTASIS
LITHOCHOLIC ACID
topic BRAIN BILE ACID PROFILE
CHOLESTASIS
LITHOCHOLIC ACID
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Introduction. Cholestasis leads to liver cell death, fibrosis, cirrhosis, and eventually liver failure. Bile duct ligated rats constitute an interesting model to study the mechanism of cholestasis, and its action on seve-ral organs and tissues, including the brain. Aim. To analyze brain bile acids individually in ligated rats to evaluate if its profile is altered towards a more toxic condition in cholestasis. Material and methods. Male Wistar rats were used and separated in two groups: bile duct ligated rats and sham operated rats (n = 5 in each group). Bile acid profile was assessed in brain homogenates. Alanine aminotransferase, aspartate ami-notransferase, alkaline phosphatase determinations, bilirubin and ammonia plasma concentration were also measured in both groups. Results. Although the total amount of bile acids in control animal brains showed a higher concentration than in bile duct ligated rats, the bile acid profile in this group was found more toxic composition than in controls. Lithocholic acid was present in brain in higher concentration (87.4% of total brain bile acids) in ligated rats and absent in controls. Alkaline phosphatase, bilirubin and ammonia were significantly higher in bile duct ligated rats than in control group. Conclusion. It was found a toxic brain bile acid profile during hepatic cholestasis which could be one of the explanations of hepatic ence-phalopathy observed in cholestatic diseases.
Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Contin, Mario Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Fernández, María AlejandrA. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiopatología; Argentina
Fil: Lemberg, Abraham. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiopatología; Argentina
description Introduction. Cholestasis leads to liver cell death, fibrosis, cirrhosis, and eventually liver failure. Bile duct ligated rats constitute an interesting model to study the mechanism of cholestasis, and its action on seve-ral organs and tissues, including the brain. Aim. To analyze brain bile acids individually in ligated rats to evaluate if its profile is altered towards a more toxic condition in cholestasis. Material and methods. Male Wistar rats were used and separated in two groups: bile duct ligated rats and sham operated rats (n = 5 in each group). Bile acid profile was assessed in brain homogenates. Alanine aminotransferase, aspartate ami-notransferase, alkaline phosphatase determinations, bilirubin and ammonia plasma concentration were also measured in both groups. Results. Although the total amount of bile acids in control animal brains showed a higher concentration than in bile duct ligated rats, the bile acid profile in this group was found more toxic composition than in controls. Lithocholic acid was present in brain in higher concentration (87.4% of total brain bile acids) in ligated rats and absent in controls. Alkaline phosphatase, bilirubin and ammonia were significantly higher in bile duct ligated rats than in control group. Conclusion. It was found a toxic brain bile acid profile during hepatic cholestasis which could be one of the explanations of hepatic ence-phalopathy observed in cholestatic diseases.
publishDate 2012
dc.date.none.fl_str_mv 2012-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/197620
Tripodi, Valeria Paula; Contin, Mario Daniel; Fernández, María AlejandrA; Lemberg, Abraham; Bile acids content in brain of common duct ligated rats; Mexican Association of Hepatology; Annals of Hepatology; 11; 6; 11-2012; 930-934
1665-2681
CONICET Digital
CONICET
url http://hdl.handle.net/11336/197620
identifier_str_mv Tripodi, Valeria Paula; Contin, Mario Daniel; Fernández, María AlejandrA; Lemberg, Abraham; Bile acids content in brain of common duct ligated rats; Mexican Association of Hepatology; Annals of Hepatology; 11; 6; 11-2012; 930-934
1665-2681
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/S1665-2681(19)31420-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mexican Association of Hepatology
publisher.none.fl_str_mv Mexican Association of Hepatology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613815480614912
score 13.070432

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