In silico selection and evaluation of pugnins with antibacterial and anticancer activity using skin transcriptome of treefrog (Boana pugnax)

Autores
Liscano, Yamil; Medina, Laura; Oñate Garzón, Jose; Gúzman, Fanny; Pickholz, Mónica Andrea; Delgado, Jean Paul
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In order to combat bacterial and cancer resistance, we identified peptides (pugnins) with dual antibacterial l–anticancer activity from the Boana pugnax (B. pugnax) skin transcriptome through in silico analysis. Pugnins A and B were selected owing to their high similarity to the DS4.3 peptide, which served as a template for their alignment to the B. pugnax transcriptome, as well as their function as part of a voltage-dependent potassium channel protein. The secondary peptide structure stability in aqueous medium was evaluated as well, and after interaction with the Escherichia coli (E. coli) membrane model using molecular dynamics. These pugnins were synthesized via solid-phase synthesis strategy and verified by Reverse phase high-performance liquid chromatography (RP-HPLC) and mass spectrometry. Subsequently, their alpha-helix structure was determined by circular dichroism, after which antibacterial tests were then performed to evaluate their antimicrobial activity. Cytotoxicity tests against cancer cells also showed selectivity of pugnin A toward breast cancer (MFC7) cells, and pugnin B toward prostate cancer (PC3) cells. Alternatively, flow cytometry revealed necrotic cell damage with a major cytotoxic effect on human keratinocytes (HaCaT) control cells. Therefore, the pugnins found in the transcriptome of B. pugnax present dual antibacterial– anticancer activity with reduced selectivity to normal eukaryotic cells.
Fil: Liscano, Yamil. Universidad Santiago de Cali; Colombia. Universidad de Antioquia; Colombia
Fil: Medina, Laura. Universidad de Antioquia; Colombia
Fil: Oñate Garzón, Jose. Universidad Santiago de Cali; Colombia
Fil: Gúzman, Fanny. Pontificia Universidad Católica de Valparaíso; Chile
Fil: Pickholz, Mónica Andrea. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Delgado, Jean Paul. Universidad de Antioquia; Colombia
Materia
ANTICANCER
ANTIMICROBIAL
BOANA PUGNAX
PEPTIDE
PUGNIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/210003
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/210003
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In silico selection and evaluation of pugnins with antibacterial and anticancer activity using skin transcriptome of treefrog (Boana pugnax)Liscano, YamilMedina, LauraOñate Garzón, JoseGúzman, FannyPickholz, Mónica AndreaDelgado, Jean PaulANTICANCERANTIMICROBIALBOANA PUGNAXPEPTIDEPUGNINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In order to combat bacterial and cancer resistance, we identified peptides (pugnins) with dual antibacterial l–anticancer activity from the Boana pugnax (B. pugnax) skin transcriptome through in silico analysis. Pugnins A and B were selected owing to their high similarity to the DS4.3 peptide, which served as a template for their alignment to the B. pugnax transcriptome, as well as their function as part of a voltage-dependent potassium channel protein. The secondary peptide structure stability in aqueous medium was evaluated as well, and after interaction with the Escherichia coli (E. coli) membrane model using molecular dynamics. These pugnins were synthesized via solid-phase synthesis strategy and verified by Reverse phase high-performance liquid chromatography (RP-HPLC) and mass spectrometry. Subsequently, their alpha-helix structure was determined by circular dichroism, after which antibacterial tests were then performed to evaluate their antimicrobial activity. Cytotoxicity tests against cancer cells also showed selectivity of pugnin A toward breast cancer (MFC7) cells, and pugnin B toward prostate cancer (PC3) cells. Alternatively, flow cytometry revealed necrotic cell damage with a major cytotoxic effect on human keratinocytes (HaCaT) control cells. Therefore, the pugnins found in the transcriptome of B. pugnax present dual antibacterial– anticancer activity with reduced selectivity to normal eukaryotic cells.Fil: Liscano, Yamil. Universidad Santiago de Cali; Colombia. Universidad de Antioquia; ColombiaFil: Medina, Laura. Universidad de Antioquia; ColombiaFil: Oñate Garzón, Jose. Universidad Santiago de Cali; ColombiaFil: Gúzman, Fanny. Pontificia Universidad Católica de Valparaíso; ChileFil: Pickholz, Mónica Andrea. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Delgado, Jean Paul. Universidad de Antioquia; ColombiaMultidisciplinary Digital Publishing Institute2021-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/210003Liscano, Yamil; Medina, Laura; Oñate Garzón, Jose; Gúzman, Fanny; Pickholz, Mónica Andrea; et al.; In silico selection and evaluation of pugnins with antibacterial and anticancer activity using skin transcriptome of treefrog (Boana pugnax); Multidisciplinary Digital Publishing Institute; Pharmaceutics; 13; 4; 4-2021; 1-321999-4923CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4923/13/4/578info:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics13040578info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:47Zoai:ri.conicet.gov.ar:11336/210003instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:48.206CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In silico selection and evaluation of pugnins with antibacterial and anticancer activity using skin transcriptome of treefrog (Boana pugnax)
title In silico selection and evaluation of pugnins with antibacterial and anticancer activity using skin transcriptome of treefrog (Boana pugnax)
spellingShingle In silico selection and evaluation of pugnins with antibacterial and anticancer activity using skin transcriptome of treefrog (Boana pugnax)
Liscano, Yamil
ANTICANCER
ANTIMICROBIAL
BOANA PUGNAX
PEPTIDE
PUGNIN
title_short In silico selection and evaluation of pugnins with antibacterial and anticancer activity using skin transcriptome of treefrog (Boana pugnax)
title_full In silico selection and evaluation of pugnins with antibacterial and anticancer activity using skin transcriptome of treefrog (Boana pugnax)
title_fullStr In silico selection and evaluation of pugnins with antibacterial and anticancer activity using skin transcriptome of treefrog (Boana pugnax)
title_full_unstemmed In silico selection and evaluation of pugnins with antibacterial and anticancer activity using skin transcriptome of treefrog (Boana pugnax)
title_sort In silico selection and evaluation of pugnins with antibacterial and anticancer activity using skin transcriptome of treefrog (Boana pugnax)
dc.creator.none.fl_str_mv Liscano, Yamil
Medina, Laura
Oñate Garzón, Jose
Gúzman, Fanny
Pickholz, Mónica Andrea
Delgado, Jean Paul
author Liscano, Yamil
author_facet Liscano, Yamil
Medina, Laura
Oñate Garzón, Jose
Gúzman, Fanny
Pickholz, Mónica Andrea
Delgado, Jean Paul
author_role author
author2 Medina, Laura
Oñate Garzón, Jose
Gúzman, Fanny
Pickholz, Mónica Andrea
Delgado, Jean Paul
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ANTICANCER
ANTIMICROBIAL
BOANA PUGNAX
PEPTIDE
PUGNIN
topic ANTICANCER
ANTIMICROBIAL
BOANA PUGNAX
PEPTIDE
PUGNIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In order to combat bacterial and cancer resistance, we identified peptides (pugnins) with dual antibacterial l–anticancer activity from the Boana pugnax (B. pugnax) skin transcriptome through in silico analysis. Pugnins A and B were selected owing to their high similarity to the DS4.3 peptide, which served as a template for their alignment to the B. pugnax transcriptome, as well as their function as part of a voltage-dependent potassium channel protein. The secondary peptide structure stability in aqueous medium was evaluated as well, and after interaction with the Escherichia coli (E. coli) membrane model using molecular dynamics. These pugnins were synthesized via solid-phase synthesis strategy and verified by Reverse phase high-performance liquid chromatography (RP-HPLC) and mass spectrometry. Subsequently, their alpha-helix structure was determined by circular dichroism, after which antibacterial tests were then performed to evaluate their antimicrobial activity. Cytotoxicity tests against cancer cells also showed selectivity of pugnin A toward breast cancer (MFC7) cells, and pugnin B toward prostate cancer (PC3) cells. Alternatively, flow cytometry revealed necrotic cell damage with a major cytotoxic effect on human keratinocytes (HaCaT) control cells. Therefore, the pugnins found in the transcriptome of B. pugnax present dual antibacterial– anticancer activity with reduced selectivity to normal eukaryotic cells.
Fil: Liscano, Yamil. Universidad Santiago de Cali; Colombia. Universidad de Antioquia; Colombia
Fil: Medina, Laura. Universidad de Antioquia; Colombia
Fil: Oñate Garzón, Jose. Universidad Santiago de Cali; Colombia
Fil: Gúzman, Fanny. Pontificia Universidad Católica de Valparaíso; Chile
Fil: Pickholz, Mónica Andrea. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Delgado, Jean Paul. Universidad de Antioquia; Colombia
description In order to combat bacterial and cancer resistance, we identified peptides (pugnins) with dual antibacterial l–anticancer activity from the Boana pugnax (B. pugnax) skin transcriptome through in silico analysis. Pugnins A and B were selected owing to their high similarity to the DS4.3 peptide, which served as a template for their alignment to the B. pugnax transcriptome, as well as their function as part of a voltage-dependent potassium channel protein. The secondary peptide structure stability in aqueous medium was evaluated as well, and after interaction with the Escherichia coli (E. coli) membrane model using molecular dynamics. These pugnins were synthesized via solid-phase synthesis strategy and verified by Reverse phase high-performance liquid chromatography (RP-HPLC) and mass spectrometry. Subsequently, their alpha-helix structure was determined by circular dichroism, after which antibacterial tests were then performed to evaluate their antimicrobial activity. Cytotoxicity tests against cancer cells also showed selectivity of pugnin A toward breast cancer (MFC7) cells, and pugnin B toward prostate cancer (PC3) cells. Alternatively, flow cytometry revealed necrotic cell damage with a major cytotoxic effect on human keratinocytes (HaCaT) control cells. Therefore, the pugnins found in the transcriptome of B. pugnax present dual antibacterial– anticancer activity with reduced selectivity to normal eukaryotic cells.
publishDate 2021
dc.date.none.fl_str_mv 2021-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/210003
Liscano, Yamil; Medina, Laura; Oñate Garzón, Jose; Gúzman, Fanny; Pickholz, Mónica Andrea; et al.; In silico selection and evaluation of pugnins with antibacterial and anticancer activity using skin transcriptome of treefrog (Boana pugnax); Multidisciplinary Digital Publishing Institute; Pharmaceutics; 13; 4; 4-2021; 1-32
1999-4923
CONICET Digital
CONICET
url http://hdl.handle.net/11336/210003
identifier_str_mv Liscano, Yamil; Medina, Laura; Oñate Garzón, Jose; Gúzman, Fanny; Pickholz, Mónica Andrea; et al.; In silico selection and evaluation of pugnins with antibacterial and anticancer activity using skin transcriptome of treefrog (Boana pugnax); Multidisciplinary Digital Publishing Institute; Pharmaceutics; 13; 4; 4-2021; 1-32
1999-4923
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4923/13/4/578
info:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics13040578
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614292605763584
score 13.070432

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