The electrogenic cardiac sodium bicarbonate co-transporter (NBCe1) contributes to the reperfusion injury

Autores
Fantinelli, Juliana Catalina; Orlowski, Alejandro; Aiello, Ernesto Alejandro; Mosca, Susana Maria
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Although the participation of the electrogenic sodium/bicarbonate cotransporter (NBCe1) in the recovery from an intracellular acid load is recognized, its role in ischemia–reperfusion is still unclear. Methods and results: Our objective was to assess the role of NBCe1 in reperfusion injury. We use selective functional antibodies against extracellular loop 3 (a-L3) and loop 4 (a-L4) of NBCe1. a-L3 inhibits and a-L4 stimulates NBCe1 activity. Isolated rat hearts were submitted to 40 min of coronary occlusion and 1 h of reperfusion. a-L3, a-L4 or S0859 — selective Na+-HCO3 - co-transport inhibitor — were administered during the initial 10 min of reperfusion. The infarct size (IS) was measured by triphenyltetrazolium chloride staining technique. Postischemic systolic and diastolic functions were also assessed. a-L3 and S0859 treatments decreased significantly (Pb.05) the IS (16±3% for a-L3 vs. 32±5% in hearts treated with control nonimmune serum and 19±3% for S0859 vs. 39±2% in untreated hearts). Myocardial function during reperfusion improved after a-L3 treatment, but it was not modified by S0859. The infusion of a-L4 did not modify neither the IS nor myocardial function. Conclusions: The NBCe1 hyperactivity during reperfusion leads to Na+ and Ca2+ loading, conducing to Ca2+ overload and myocardial damage. Consistently, we have shown herein that the selective NBCe1 blockade with a-L3 exerted cardioprotection. This beneficial action strongly suggests that NBCe1 could be a potential target for the treatment of coronary disease.
Fil: Fantinelli, Juliana Catalina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
Fil: Orlowski, Alejandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
Fil: Aiello, Ernesto Alejandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
Fil: Mosca, Susana Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
Materia
Nbce1
Reperfusion Injury
Sodium Bicarbonate Co-Transporter
S0859
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11942
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/11942
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The electrogenic cardiac sodium bicarbonate co-transporter (NBCe1) contributes to the reperfusion injuryFantinelli, Juliana CatalinaOrlowski, AlejandroAiello, Ernesto AlejandroMosca, Susana MariaNbce1Reperfusion InjurySodium Bicarbonate Co-TransporterS0859https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: Although the participation of the electrogenic sodium/bicarbonate cotransporter (NBCe1) in the recovery from an intracellular acid load is recognized, its role in ischemia–reperfusion is still unclear. Methods and results: Our objective was to assess the role of NBCe1 in reperfusion injury. We use selective functional antibodies against extracellular loop 3 (a-L3) and loop 4 (a-L4) of NBCe1. a-L3 inhibits and a-L4 stimulates NBCe1 activity. Isolated rat hearts were submitted to 40 min of coronary occlusion and 1 h of reperfusion. a-L3, a-L4 or S0859 — selective Na+-HCO3 - co-transport inhibitor — were administered during the initial 10 min of reperfusion. The infarct size (IS) was measured by triphenyltetrazolium chloride staining technique. Postischemic systolic and diastolic functions were also assessed. a-L3 and S0859 treatments decreased significantly (Pb.05) the IS (16±3% for a-L3 vs. 32±5% in hearts treated with control nonimmune serum and 19±3% for S0859 vs. 39±2% in untreated hearts). Myocardial function during reperfusion improved after a-L3 treatment, but it was not modified by S0859. The infusion of a-L4 did not modify neither the IS nor myocardial function. Conclusions: The NBCe1 hyperactivity during reperfusion leads to Na+ and Ca2+ loading, conducing to Ca2+ overload and myocardial damage. Consistently, we have shown herein that the selective NBCe1 blockade with a-L3 exerted cardioprotection. This beneficial action strongly suggests that NBCe1 could be a potential target for the treatment of coronary disease.Fil: Fantinelli, Juliana Catalina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; ArgentinaFil: Orlowski, Alejandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; ArgentinaFil: Aiello, Ernesto Alejandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; ArgentinaFil: Mosca, Susana Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; ArgentinaElsevier Science Inc2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11942Fantinelli, Juliana Catalina; Orlowski, Alejandro; Aiello, Ernesto Alejandro; Mosca, Susana Maria; The electrogenic cardiac sodium bicarbonate co-transporter (NBCe1) contributes to the reperfusion injury; Elsevier Science Inc; Cardiovascular Pathology; 23; 4; 7-2014; 224-2301054-8807enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.carpath.2014.03.003info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1054880714000313info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:15Zoai:ri.conicet.gov.ar:11336/11942instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:16.18CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The electrogenic cardiac sodium bicarbonate co-transporter (NBCe1) contributes to the reperfusion injury
title The electrogenic cardiac sodium bicarbonate co-transporter (NBCe1) contributes to the reperfusion injury
spellingShingle The electrogenic cardiac sodium bicarbonate co-transporter (NBCe1) contributes to the reperfusion injury
Fantinelli, Juliana Catalina
Nbce1
Reperfusion Injury
Sodium Bicarbonate Co-Transporter
S0859
title_short The electrogenic cardiac sodium bicarbonate co-transporter (NBCe1) contributes to the reperfusion injury
title_full The electrogenic cardiac sodium bicarbonate co-transporter (NBCe1) contributes to the reperfusion injury
title_fullStr The electrogenic cardiac sodium bicarbonate co-transporter (NBCe1) contributes to the reperfusion injury
title_full_unstemmed The electrogenic cardiac sodium bicarbonate co-transporter (NBCe1) contributes to the reperfusion injury
title_sort The electrogenic cardiac sodium bicarbonate co-transporter (NBCe1) contributes to the reperfusion injury
dc.creator.none.fl_str_mv Fantinelli, Juliana Catalina
Orlowski, Alejandro
Aiello, Ernesto Alejandro
Mosca, Susana Maria
author Fantinelli, Juliana Catalina
author_facet Fantinelli, Juliana Catalina
Orlowski, Alejandro
Aiello, Ernesto Alejandro
Mosca, Susana Maria
author_role author
author2 Orlowski, Alejandro
Aiello, Ernesto Alejandro
Mosca, Susana Maria
author2_role author
author
author
dc.subject.none.fl_str_mv Nbce1
Reperfusion Injury
Sodium Bicarbonate Co-Transporter
S0859
topic Nbce1
Reperfusion Injury
Sodium Bicarbonate Co-Transporter
S0859
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Although the participation of the electrogenic sodium/bicarbonate cotransporter (NBCe1) in the recovery from an intracellular acid load is recognized, its role in ischemia–reperfusion is still unclear. Methods and results: Our objective was to assess the role of NBCe1 in reperfusion injury. We use selective functional antibodies against extracellular loop 3 (a-L3) and loop 4 (a-L4) of NBCe1. a-L3 inhibits and a-L4 stimulates NBCe1 activity. Isolated rat hearts were submitted to 40 min of coronary occlusion and 1 h of reperfusion. a-L3, a-L4 or S0859 — selective Na+-HCO3 - co-transport inhibitor — were administered during the initial 10 min of reperfusion. The infarct size (IS) was measured by triphenyltetrazolium chloride staining technique. Postischemic systolic and diastolic functions were also assessed. a-L3 and S0859 treatments decreased significantly (Pb.05) the IS (16±3% for a-L3 vs. 32±5% in hearts treated with control nonimmune serum and 19±3% for S0859 vs. 39±2% in untreated hearts). Myocardial function during reperfusion improved after a-L3 treatment, but it was not modified by S0859. The infusion of a-L4 did not modify neither the IS nor myocardial function. Conclusions: The NBCe1 hyperactivity during reperfusion leads to Na+ and Ca2+ loading, conducing to Ca2+ overload and myocardial damage. Consistently, we have shown herein that the selective NBCe1 blockade with a-L3 exerted cardioprotection. This beneficial action strongly suggests that NBCe1 could be a potential target for the treatment of coronary disease.
Fil: Fantinelli, Juliana Catalina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
Fil: Orlowski, Alejandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
Fil: Aiello, Ernesto Alejandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
Fil: Mosca, Susana Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico la Plata. Centro de Investigaciones Cardiovasculares "dr. Horacio Eugenio Cingolani"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas; Argentina
description Background: Although the participation of the electrogenic sodium/bicarbonate cotransporter (NBCe1) in the recovery from an intracellular acid load is recognized, its role in ischemia–reperfusion is still unclear. Methods and results: Our objective was to assess the role of NBCe1 in reperfusion injury. We use selective functional antibodies against extracellular loop 3 (a-L3) and loop 4 (a-L4) of NBCe1. a-L3 inhibits and a-L4 stimulates NBCe1 activity. Isolated rat hearts were submitted to 40 min of coronary occlusion and 1 h of reperfusion. a-L3, a-L4 or S0859 — selective Na+-HCO3 - co-transport inhibitor — were administered during the initial 10 min of reperfusion. The infarct size (IS) was measured by triphenyltetrazolium chloride staining technique. Postischemic systolic and diastolic functions were also assessed. a-L3 and S0859 treatments decreased significantly (Pb.05) the IS (16±3% for a-L3 vs. 32±5% in hearts treated with control nonimmune serum and 19±3% for S0859 vs. 39±2% in untreated hearts). Myocardial function during reperfusion improved after a-L3 treatment, but it was not modified by S0859. The infusion of a-L4 did not modify neither the IS nor myocardial function. Conclusions: The NBCe1 hyperactivity during reperfusion leads to Na+ and Ca2+ loading, conducing to Ca2+ overload and myocardial damage. Consistently, we have shown herein that the selective NBCe1 blockade with a-L3 exerted cardioprotection. This beneficial action strongly suggests that NBCe1 could be a potential target for the treatment of coronary disease.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11942
Fantinelli, Juliana Catalina; Orlowski, Alejandro; Aiello, Ernesto Alejandro; Mosca, Susana Maria; The electrogenic cardiac sodium bicarbonate co-transporter (NBCe1) contributes to the reperfusion injury; Elsevier Science Inc; Cardiovascular Pathology; 23; 4; 7-2014; 224-230
1054-8807
url http://hdl.handle.net/11336/11942
identifier_str_mv Fantinelli, Juliana Catalina; Orlowski, Alejandro; Aiello, Ernesto Alejandro; Mosca, Susana Maria; The electrogenic cardiac sodium bicarbonate co-transporter (NBCe1) contributes to the reperfusion injury; Elsevier Science Inc; Cardiovascular Pathology; 23; 4; 7-2014; 224-230
1054-8807
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carpath.2014.03.003
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1054880714000313
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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