Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum

Autores
Cano, María Emilia; Lindgren, Åsa; Rosendahl, Jennifer; Johansson, Jenny; García Martin, Alberto; Ladero Galán, Miguel; Kovensky, Jose Eduardo; Chinga Carrasco, Gary
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Biomass-derived oligo- and polysaccharides may act as elicitors, i.e., bioactive molecules that trigger plant immune responses. This is particularly important to increase the resistance of plants to abiotic and biotic stresses. In this study, cellulose nanofibrils (CNF) gels were obtained by TEMPO-mediated oxidation of unbleached and bleached kraft pulps. The molecular structures were characterized with ESI and MALDI MS. Analysis of the fine sequences was achieved by MS and MS/MS of the water-soluble oligosaccharides obtained by acid hydrolysis of the CNF gels. The analysis revealed the presence of two families: one corresponding to homoglucuronic acid sequences and the other composed by alternating glucose and glucuronic acid units. The CNF gels, alone or with the addition of the water-soluble oligosaccharides, were tested on Chili pepper (Capsicum annuum). Based on the characterization of the gene expression with Next Generation Sequencing (NGS) of the C. annuum´s total messenger RNA, the differences in growth of the C. annuum seeds correlated well with the downregulation of the pathways regulating photosynthesis. A downregulation of the response to abiotic factors was detected, suggesting that these gels would improve the resistance of the C. annuum plants to abiotic stress due to, e.g., water deprivation and cold temperatures.
Fil: Cano, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universite de Picardie Jules Verne (universite de Picardie Jules V);
Fil: Lindgren, Åsa. Research Institutes of Sweden; Suecia
Fil: Rosendahl, Jennifer. Research Institutes of Sweden; Suecia
Fil: Johansson, Jenny. Research Institutes of Sweden; Suecia
Fil: García Martin, Alberto. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Ladero Galán, Miguel. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Kovensky, Jose Eduardo. Universite de Picardie Jules Verne (universite de Picardie Jules V);
Fil: Chinga Carrasco, Gary. Paper and Fibre Research Institute; Noruega
Materia
NANOCELLULOSE
TEMPO-OXIDIZED NANOFIBRILS
ELICITOR
OLIGOSACCHARIDES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/261046
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/261046
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuumCano, María EmiliaLindgren, ÅsaRosendahl, JenniferJohansson, JennyGarcía Martin, AlbertoLadero Galán, MiguelKovensky, Jose EduardoChinga Carrasco, GaryNANOCELLULOSETEMPO-OXIDIZED NANOFIBRILSELICITOROLIGOSACCHARIDEShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Biomass-derived oligo- and polysaccharides may act as elicitors, i.e., bioactive molecules that trigger plant immune responses. This is particularly important to increase the resistance of plants to abiotic and biotic stresses. In this study, cellulose nanofibrils (CNF) gels were obtained by TEMPO-mediated oxidation of unbleached and bleached kraft pulps. The molecular structures were characterized with ESI and MALDI MS. Analysis of the fine sequences was achieved by MS and MS/MS of the water-soluble oligosaccharides obtained by acid hydrolysis of the CNF gels. The analysis revealed the presence of two families: one corresponding to homoglucuronic acid sequences and the other composed by alternating glucose and glucuronic acid units. The CNF gels, alone or with the addition of the water-soluble oligosaccharides, were tested on Chili pepper (Capsicum annuum). Based on the characterization of the gene expression with Next Generation Sequencing (NGS) of the C. annuum´s total messenger RNA, the differences in growth of the C. annuum seeds correlated well with the downregulation of the pathways regulating photosynthesis. A downregulation of the response to abiotic factors was detected, suggesting that these gels would improve the resistance of the C. annuum plants to abiotic stress due to, e.g., water deprivation and cold temperatures.Fil: Cano, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universite de Picardie Jules Verne (universite de Picardie Jules V);Fil: Lindgren, Åsa. Research Institutes of Sweden; SueciaFil: Rosendahl, Jennifer. Research Institutes of Sweden; SueciaFil: Johansson, Jenny. Research Institutes of Sweden; SueciaFil: García Martin, Alberto. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Ladero Galán, Miguel. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Kovensky, Jose Eduardo. Universite de Picardie Jules Verne (universite de Picardie Jules V);Fil: Chinga Carrasco, Gary. Paper and Fibre Research Institute; NoruegaElsevier Science2024-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/261046Cano, María Emilia; Lindgren, Åsa; Rosendahl, Jennifer; Johansson, Jenny; García Martin, Alberto; et al.; Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum; Elsevier Science; International Journal of Biological Macromolecules; 267; 4-2024; 1-110141-81301879-0003CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0141813024020348info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2024.131229info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:05Zoai:ri.conicet.gov.ar:11336/261046instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:05.731CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum
title Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum
spellingShingle Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum
Cano, María Emilia
NANOCELLULOSE
TEMPO-OXIDIZED NANOFIBRILS
ELICITOR
OLIGOSACCHARIDES
title_short Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum
title_full Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum
title_fullStr Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum
title_full_unstemmed Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum
title_sort Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum
dc.creator.none.fl_str_mv Cano, María Emilia
Lindgren, Åsa
Rosendahl, Jennifer
Johansson, Jenny
García Martin, Alberto
Ladero Galán, Miguel
Kovensky, Jose Eduardo
Chinga Carrasco, Gary
author Cano, María Emilia
author_facet Cano, María Emilia
Lindgren, Åsa
Rosendahl, Jennifer
Johansson, Jenny
García Martin, Alberto
Ladero Galán, Miguel
Kovensky, Jose Eduardo
Chinga Carrasco, Gary
author_role author
author2 Lindgren, Åsa
Rosendahl, Jennifer
Johansson, Jenny
García Martin, Alberto
Ladero Galán, Miguel
Kovensky, Jose Eduardo
Chinga Carrasco, Gary
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv NANOCELLULOSE
TEMPO-OXIDIZED NANOFIBRILS
ELICITOR
OLIGOSACCHARIDES
topic NANOCELLULOSE
TEMPO-OXIDIZED NANOFIBRILS
ELICITOR
OLIGOSACCHARIDES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Biomass-derived oligo- and polysaccharides may act as elicitors, i.e., bioactive molecules that trigger plant immune responses. This is particularly important to increase the resistance of plants to abiotic and biotic stresses. In this study, cellulose nanofibrils (CNF) gels were obtained by TEMPO-mediated oxidation of unbleached and bleached kraft pulps. The molecular structures were characterized with ESI and MALDI MS. Analysis of the fine sequences was achieved by MS and MS/MS of the water-soluble oligosaccharides obtained by acid hydrolysis of the CNF gels. The analysis revealed the presence of two families: one corresponding to homoglucuronic acid sequences and the other composed by alternating glucose and glucuronic acid units. The CNF gels, alone or with the addition of the water-soluble oligosaccharides, were tested on Chili pepper (Capsicum annuum). Based on the characterization of the gene expression with Next Generation Sequencing (NGS) of the C. annuum´s total messenger RNA, the differences in growth of the C. annuum seeds correlated well with the downregulation of the pathways regulating photosynthesis. A downregulation of the response to abiotic factors was detected, suggesting that these gels would improve the resistance of the C. annuum plants to abiotic stress due to, e.g., water deprivation and cold temperatures.
Fil: Cano, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina. Universite de Picardie Jules Verne (universite de Picardie Jules V);
Fil: Lindgren, Åsa. Research Institutes of Sweden; Suecia
Fil: Rosendahl, Jennifer. Research Institutes of Sweden; Suecia
Fil: Johansson, Jenny. Research Institutes of Sweden; Suecia
Fil: García Martin, Alberto. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Ladero Galán, Miguel. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Kovensky, Jose Eduardo. Universite de Picardie Jules Verne (universite de Picardie Jules V);
Fil: Chinga Carrasco, Gary. Paper and Fibre Research Institute; Noruega
description Biomass-derived oligo- and polysaccharides may act as elicitors, i.e., bioactive molecules that trigger plant immune responses. This is particularly important to increase the resistance of plants to abiotic and biotic stresses. In this study, cellulose nanofibrils (CNF) gels were obtained by TEMPO-mediated oxidation of unbleached and bleached kraft pulps. The molecular structures were characterized with ESI and MALDI MS. Analysis of the fine sequences was achieved by MS and MS/MS of the water-soluble oligosaccharides obtained by acid hydrolysis of the CNF gels. The analysis revealed the presence of two families: one corresponding to homoglucuronic acid sequences and the other composed by alternating glucose and glucuronic acid units. The CNF gels, alone or with the addition of the water-soluble oligosaccharides, were tested on Chili pepper (Capsicum annuum). Based on the characterization of the gene expression with Next Generation Sequencing (NGS) of the C. annuum´s total messenger RNA, the differences in growth of the C. annuum seeds correlated well with the downregulation of the pathways regulating photosynthesis. A downregulation of the response to abiotic factors was detected, suggesting that these gels would improve the resistance of the C. annuum plants to abiotic stress due to, e.g., water deprivation and cold temperatures.
publishDate 2024
dc.date.none.fl_str_mv 2024-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/261046
Cano, María Emilia; Lindgren, Åsa; Rosendahl, Jennifer; Johansson, Jenny; García Martin, Alberto; et al.; Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum; Elsevier Science; International Journal of Biological Macromolecules; 267; 4-2024; 1-11
0141-8130
1879-0003
CONICET Digital
CONICET
url http://hdl.handle.net/11336/261046
identifier_str_mv Cano, María Emilia; Lindgren, Åsa; Rosendahl, Jennifer; Johansson, Jenny; García Martin, Alberto; et al.; Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum; Elsevier Science; International Journal of Biological Macromolecules; 267; 4-2024; 1-11
0141-8130
1879-0003
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0141813024020348
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2024.131229
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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