Seasonal Morphological Variations and Age-Related Changes of the Seminal Vesicle of Viscacha (Lagostomus maximus maximus): An Ultrastructural and Immunohistochemical Study

Autores
Chaves, Eduardo Maximiliano; Aguilera Merlo, Claudia Isabel; Cruceño, Albana Andrea Marina; Fogal, Teresa Hilda; Piezzi, Ramon Salvador; Scardapane, Luis Antonio; Dominguez, Susana
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The viscacha is a seasonal rodent that exhibit an annual reproductive cycle with periods of maximum reproductive activity and gonadal regression. We studied seasonal variations in the morphology and cellular population of the seminal vesicles (SVs) during both periods and in impuber animals. Seminal vesicles were studied by light and electronic microscopy. Measurements of epithelial height, nuclear diameter, luminal diameter, and muscular layer were performed. Also, we studied the distribution of androgen receptors (AR) in this gland during the reproductive cycle and in impuber animal. During gonadal regression, principal and clear cells showed signs of reduced functional activity. These were characterized by an epithelium of smaller height, irregular nuclei, and cytoplasm with few organelles, dilated cisterns, and glycogen granules. In impuber animals, the principal cells showed large nuclei with chromatin lax and cytoplasm with small mitochondria, poorly developed Golgi apparatus, and granules of glycogen. On the other hand, the cells exhibited seasonal variations in the distribution and percentage of immunolabeled cells to AR throughout the annual reproductive cycle. During the gonadal regression period, glandular mucosa exhibited numerous epithelial cells with intense nuclear staining. However, fibromuscular stromal cells were weakly positive for AR in contrast to what was observed during the activity period. Considering that testosterone values are lower in adult animals during the period of gonadal regression and in impuber animals, our immunohistochemical results show a significant correlation with the percentage of AR-immunopositive cells. In conclusion, these results demonstrate that the structure of the SVs changes in the activity period of viscacha, probably because of elevated levels of testosterone leading to an increase in the secretory activity of epithelial cells.
Fil: Chaves, Eduardo Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Aguilera Merlo, Claudia Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Cruceño, Albana Andrea Marina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Fogal, Teresa Hilda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Piezzi, Ramon Salvador. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Scardapane, Luis Antonio. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Dominguez, Susana. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Materia
ANDROGEN RECEPTOR
IMPUBER
SEASONAL REPRODUCTION
SEMINAL VESICLES
TESTOSTERONE LEVELS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/216461
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/216461
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Seasonal Morphological Variations and Age-Related Changes of the Seminal Vesicle of Viscacha (Lagostomus maximus maximus): An Ultrastructural and Immunohistochemical StudyChaves, Eduardo MaximilianoAguilera Merlo, Claudia IsabelCruceño, Albana Andrea MarinaFogal, Teresa HildaPiezzi, Ramon SalvadorScardapane, Luis AntonioDominguez, SusanaANDROGEN RECEPTORIMPUBERSEASONAL REPRODUCTIONSEMINAL VESICLESTESTOSTERONE LEVELShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The viscacha is a seasonal rodent that exhibit an annual reproductive cycle with periods of maximum reproductive activity and gonadal regression. We studied seasonal variations in the morphology and cellular population of the seminal vesicles (SVs) during both periods and in impuber animals. Seminal vesicles were studied by light and electronic microscopy. Measurements of epithelial height, nuclear diameter, luminal diameter, and muscular layer were performed. Also, we studied the distribution of androgen receptors (AR) in this gland during the reproductive cycle and in impuber animal. During gonadal regression, principal and clear cells showed signs of reduced functional activity. These were characterized by an epithelium of smaller height, irregular nuclei, and cytoplasm with few organelles, dilated cisterns, and glycogen granules. In impuber animals, the principal cells showed large nuclei with chromatin lax and cytoplasm with small mitochondria, poorly developed Golgi apparatus, and granules of glycogen. On the other hand, the cells exhibited seasonal variations in the distribution and percentage of immunolabeled cells to AR throughout the annual reproductive cycle. During the gonadal regression period, glandular mucosa exhibited numerous epithelial cells with intense nuclear staining. However, fibromuscular stromal cells were weakly positive for AR in contrast to what was observed during the activity period. Considering that testosterone values are lower in adult animals during the period of gonadal regression and in impuber animals, our immunohistochemical results show a significant correlation with the percentage of AR-immunopositive cells. In conclusion, these results demonstrate that the structure of the SVs changes in the activity period of viscacha, probably because of elevated levels of testosterone leading to an increase in the secretory activity of epithelial cells.Fil: Chaves, Eduardo Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Aguilera Merlo, Claudia Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Cruceño, Albana Andrea Marina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Fogal, Teresa Hilda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Piezzi, Ramon Salvador. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Scardapane, Luis Antonio. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Dominguez, Susana. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaWiley-liss, div John Wiley & Sons Inc.2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/216461Chaves, Eduardo Maximiliano; Aguilera Merlo, Claudia Isabel; Cruceño, Albana Andrea Marina; Fogal, Teresa Hilda; Piezzi, Ramon Salvador; et al.; Seasonal Morphological Variations and Age-Related Changes of the Seminal Vesicle of Viscacha (Lagostomus maximus maximus): An Ultrastructural and Immunohistochemical Study; Wiley-liss, div John Wiley & Sons Inc.; Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; 295; 5; 2-2012; 886-8951932-8486CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://anatomypubs.onlinelibrary.wiley.com/doi/full/10.1002/ar.22434info:eu-repo/semantics/altIdentifier/doi/10.1002/ar.22434info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:24:30Zoai:ri.conicet.gov.ar:11336/216461instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:24:30.795CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Seasonal Morphological Variations and Age-Related Changes of the Seminal Vesicle of Viscacha (Lagostomus maximus maximus): An Ultrastructural and Immunohistochemical Study
title Seasonal Morphological Variations and Age-Related Changes of the Seminal Vesicle of Viscacha (Lagostomus maximus maximus): An Ultrastructural and Immunohistochemical Study
spellingShingle Seasonal Morphological Variations and Age-Related Changes of the Seminal Vesicle of Viscacha (Lagostomus maximus maximus): An Ultrastructural and Immunohistochemical Study
Chaves, Eduardo Maximiliano
ANDROGEN RECEPTOR
IMPUBER
SEASONAL REPRODUCTION
SEMINAL VESICLES
TESTOSTERONE LEVELS
title_short Seasonal Morphological Variations and Age-Related Changes of the Seminal Vesicle of Viscacha (Lagostomus maximus maximus): An Ultrastructural and Immunohistochemical Study
title_full Seasonal Morphological Variations and Age-Related Changes of the Seminal Vesicle of Viscacha (Lagostomus maximus maximus): An Ultrastructural and Immunohistochemical Study
title_fullStr Seasonal Morphological Variations and Age-Related Changes of the Seminal Vesicle of Viscacha (Lagostomus maximus maximus): An Ultrastructural and Immunohistochemical Study
title_full_unstemmed Seasonal Morphological Variations and Age-Related Changes of the Seminal Vesicle of Viscacha (Lagostomus maximus maximus): An Ultrastructural and Immunohistochemical Study
title_sort Seasonal Morphological Variations and Age-Related Changes of the Seminal Vesicle of Viscacha (Lagostomus maximus maximus): An Ultrastructural and Immunohistochemical Study
dc.creator.none.fl_str_mv Chaves, Eduardo Maximiliano
Aguilera Merlo, Claudia Isabel
Cruceño, Albana Andrea Marina
Fogal, Teresa Hilda
Piezzi, Ramon Salvador
Scardapane, Luis Antonio
Dominguez, Susana
author Chaves, Eduardo Maximiliano
author_facet Chaves, Eduardo Maximiliano
Aguilera Merlo, Claudia Isabel
Cruceño, Albana Andrea Marina
Fogal, Teresa Hilda
Piezzi, Ramon Salvador
Scardapane, Luis Antonio
Dominguez, Susana
author_role author
author2 Aguilera Merlo, Claudia Isabel
Cruceño, Albana Andrea Marina
Fogal, Teresa Hilda
Piezzi, Ramon Salvador
Scardapane, Luis Antonio
Dominguez, Susana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANDROGEN RECEPTOR
IMPUBER
SEASONAL REPRODUCTION
SEMINAL VESICLES
TESTOSTERONE LEVELS
topic ANDROGEN RECEPTOR
IMPUBER
SEASONAL REPRODUCTION
SEMINAL VESICLES
TESTOSTERONE LEVELS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The viscacha is a seasonal rodent that exhibit an annual reproductive cycle with periods of maximum reproductive activity and gonadal regression. We studied seasonal variations in the morphology and cellular population of the seminal vesicles (SVs) during both periods and in impuber animals. Seminal vesicles were studied by light and electronic microscopy. Measurements of epithelial height, nuclear diameter, luminal diameter, and muscular layer were performed. Also, we studied the distribution of androgen receptors (AR) in this gland during the reproductive cycle and in impuber animal. During gonadal regression, principal and clear cells showed signs of reduced functional activity. These were characterized by an epithelium of smaller height, irregular nuclei, and cytoplasm with few organelles, dilated cisterns, and glycogen granules. In impuber animals, the principal cells showed large nuclei with chromatin lax and cytoplasm with small mitochondria, poorly developed Golgi apparatus, and granules of glycogen. On the other hand, the cells exhibited seasonal variations in the distribution and percentage of immunolabeled cells to AR throughout the annual reproductive cycle. During the gonadal regression period, glandular mucosa exhibited numerous epithelial cells with intense nuclear staining. However, fibromuscular stromal cells were weakly positive for AR in contrast to what was observed during the activity period. Considering that testosterone values are lower in adult animals during the period of gonadal regression and in impuber animals, our immunohistochemical results show a significant correlation with the percentage of AR-immunopositive cells. In conclusion, these results demonstrate that the structure of the SVs changes in the activity period of viscacha, probably because of elevated levels of testosterone leading to an increase in the secretory activity of epithelial cells.
Fil: Chaves, Eduardo Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Aguilera Merlo, Claudia Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Cruceño, Albana Andrea Marina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Fogal, Teresa Hilda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Piezzi, Ramon Salvador. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Scardapane, Luis Antonio. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Dominguez, Susana. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
description The viscacha is a seasonal rodent that exhibit an annual reproductive cycle with periods of maximum reproductive activity and gonadal regression. We studied seasonal variations in the morphology and cellular population of the seminal vesicles (SVs) during both periods and in impuber animals. Seminal vesicles were studied by light and electronic microscopy. Measurements of epithelial height, nuclear diameter, luminal diameter, and muscular layer were performed. Also, we studied the distribution of androgen receptors (AR) in this gland during the reproductive cycle and in impuber animal. During gonadal regression, principal and clear cells showed signs of reduced functional activity. These were characterized by an epithelium of smaller height, irregular nuclei, and cytoplasm with few organelles, dilated cisterns, and glycogen granules. In impuber animals, the principal cells showed large nuclei with chromatin lax and cytoplasm with small mitochondria, poorly developed Golgi apparatus, and granules of glycogen. On the other hand, the cells exhibited seasonal variations in the distribution and percentage of immunolabeled cells to AR throughout the annual reproductive cycle. During the gonadal regression period, glandular mucosa exhibited numerous epithelial cells with intense nuclear staining. However, fibromuscular stromal cells were weakly positive for AR in contrast to what was observed during the activity period. Considering that testosterone values are lower in adult animals during the period of gonadal regression and in impuber animals, our immunohistochemical results show a significant correlation with the percentage of AR-immunopositive cells. In conclusion, these results demonstrate that the structure of the SVs changes in the activity period of viscacha, probably because of elevated levels of testosterone leading to an increase in the secretory activity of epithelial cells.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/216461
Chaves, Eduardo Maximiliano; Aguilera Merlo, Claudia Isabel; Cruceño, Albana Andrea Marina; Fogal, Teresa Hilda; Piezzi, Ramon Salvador; et al.; Seasonal Morphological Variations and Age-Related Changes of the Seminal Vesicle of Viscacha (Lagostomus maximus maximus): An Ultrastructural and Immunohistochemical Study; Wiley-liss, div John Wiley & Sons Inc.; Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; 295; 5; 2-2012; 886-895
1932-8486
CONICET Digital
CONICET
url http://hdl.handle.net/11336/216461
identifier_str_mv Chaves, Eduardo Maximiliano; Aguilera Merlo, Claudia Isabel; Cruceño, Albana Andrea Marina; Fogal, Teresa Hilda; Piezzi, Ramon Salvador; et al.; Seasonal Morphological Variations and Age-Related Changes of the Seminal Vesicle of Viscacha (Lagostomus maximus maximus): An Ultrastructural and Immunohistochemical Study; Wiley-liss, div John Wiley & Sons Inc.; Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; 295; 5; 2-2012; 886-895
1932-8486
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://anatomypubs.onlinelibrary.wiley.com/doi/full/10.1002/ar.22434
info:eu-repo/semantics/altIdentifier/doi/10.1002/ar.22434
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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