Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatin capsules

Autores
Zarate, Gabriela del Valle; Nader, Maria Elena Fatima
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present study we investigated the survival and stability of probiotic properties of three vaginal lactobacilli after freeze drying with the addition of individual and combined compounds commonly used as pharmaceutical excipients and/or starters cryoprotectants, and subsequent refrigerated storage during 15 months. Lactobacilli, suspended in lactose, skim milk, ascorbic acid and combinations of them, were freeze-dried, packed into gelatin capsules and stored at 5 °C under darkness. At regular intervals throughout storage, freeze-dried samples were rehydrated and viability, adhesion to vaginal epithelial cells and antimicrobial activities were evaluated. The Lactobacillus tested conserved high viability up to 12 months in capsules containing ascorbic acid or combined excipients whereas lyophilization and storage with lactose or skim milk significantly decreased their survival. Abilities to produce lactic acid, H2O2 and bacteriocin were affected to different extents depending on the condition assayed. Lyophilization and storage also reduced the capacity of lactobacilli to adhere to vaginal epithelial cells but this property was partially restored after the first subculture in broth. The results obtained suggests that suitable selection of excipients that could also act as protecting agents of LAB during storage should be a valuable step in the development of a probiotic formulation with a stable shelf-life.
Fil: Zarate, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Materia
Lactobacillus
Lyophilization
Probiotic
Storage
Survival
Vagina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36578
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatin capsulesZarate, Gabriela del ValleNader, Maria Elena FatimaLactobacillusLyophilizationProbioticStorageSurvivalVaginahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the present study we investigated the survival and stability of probiotic properties of three vaginal lactobacilli after freeze drying with the addition of individual and combined compounds commonly used as pharmaceutical excipients and/or starters cryoprotectants, and subsequent refrigerated storage during 15 months. Lactobacilli, suspended in lactose, skim milk, ascorbic acid and combinations of them, were freeze-dried, packed into gelatin capsules and stored at 5 °C under darkness. At regular intervals throughout storage, freeze-dried samples were rehydrated and viability, adhesion to vaginal epithelial cells and antimicrobial activities were evaluated. The Lactobacillus tested conserved high viability up to 12 months in capsules containing ascorbic acid or combined excipients whereas lyophilization and storage with lactose or skim milk significantly decreased their survival. Abilities to produce lactic acid, H2O2 and bacteriocin were affected to different extents depending on the condition assayed. Lyophilization and storage also reduced the capacity of lactobacilli to adhere to vaginal epithelial cells but this property was partially restored after the first subculture in broth. The results obtained suggests that suitable selection of excipients that could also act as protecting agents of LAB during storage should be a valuable step in the development of a probiotic formulation with a stable shelf-life.Fil: Zarate, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaElsevier2006-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36578Zarate, Gabriela del Valle; Nader, Maria Elena Fatima; Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatin capsules; Elsevier; Process Biochemistry; 41; 8; 8-2006; 1779-17851359-5113CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2006.03.024info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S135951130600122Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:33Zoai:ri.conicet.gov.ar:11336/36578instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:33.396CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatin capsules
title Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatin capsules
spellingShingle Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatin capsules
Zarate, Gabriela del Valle
Lactobacillus
Lyophilization
Probiotic
Storage
Survival
Vagina
title_short Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatin capsules
title_full Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatin capsules
title_fullStr Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatin capsules
title_full_unstemmed Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatin capsules
title_sort Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatin capsules
dc.creator.none.fl_str_mv Zarate, Gabriela del Valle
Nader, Maria Elena Fatima
author Zarate, Gabriela del Valle
author_facet Zarate, Gabriela del Valle
Nader, Maria Elena Fatima
author_role author
author2 Nader, Maria Elena Fatima
author2_role author
dc.subject.none.fl_str_mv Lactobacillus
Lyophilization
Probiotic
Storage
Survival
Vagina
topic Lactobacillus
Lyophilization
Probiotic
Storage
Survival
Vagina
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the present study we investigated the survival and stability of probiotic properties of three vaginal lactobacilli after freeze drying with the addition of individual and combined compounds commonly used as pharmaceutical excipients and/or starters cryoprotectants, and subsequent refrigerated storage during 15 months. Lactobacilli, suspended in lactose, skim milk, ascorbic acid and combinations of them, were freeze-dried, packed into gelatin capsules and stored at 5 °C under darkness. At regular intervals throughout storage, freeze-dried samples were rehydrated and viability, adhesion to vaginal epithelial cells and antimicrobial activities were evaluated. The Lactobacillus tested conserved high viability up to 12 months in capsules containing ascorbic acid or combined excipients whereas lyophilization and storage with lactose or skim milk significantly decreased their survival. Abilities to produce lactic acid, H2O2 and bacteriocin were affected to different extents depending on the condition assayed. Lyophilization and storage also reduced the capacity of lactobacilli to adhere to vaginal epithelial cells but this property was partially restored after the first subculture in broth. The results obtained suggests that suitable selection of excipients that could also act as protecting agents of LAB during storage should be a valuable step in the development of a probiotic formulation with a stable shelf-life.
Fil: Zarate, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
description In the present study we investigated the survival and stability of probiotic properties of three vaginal lactobacilli after freeze drying with the addition of individual and combined compounds commonly used as pharmaceutical excipients and/or starters cryoprotectants, and subsequent refrigerated storage during 15 months. Lactobacilli, suspended in lactose, skim milk, ascorbic acid and combinations of them, were freeze-dried, packed into gelatin capsules and stored at 5 °C under darkness. At regular intervals throughout storage, freeze-dried samples were rehydrated and viability, adhesion to vaginal epithelial cells and antimicrobial activities were evaluated. The Lactobacillus tested conserved high viability up to 12 months in capsules containing ascorbic acid or combined excipients whereas lyophilization and storage with lactose or skim milk significantly decreased their survival. Abilities to produce lactic acid, H2O2 and bacteriocin were affected to different extents depending on the condition assayed. Lyophilization and storage also reduced the capacity of lactobacilli to adhere to vaginal epithelial cells but this property was partially restored after the first subculture in broth. The results obtained suggests that suitable selection of excipients that could also act as protecting agents of LAB during storage should be a valuable step in the development of a probiotic formulation with a stable shelf-life.
publishDate 2006
dc.date.none.fl_str_mv 2006-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36578
Zarate, Gabriela del Valle; Nader, Maria Elena Fatima; Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatin capsules; Elsevier; Process Biochemistry; 41; 8; 8-2006; 1779-1785
1359-5113
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36578
identifier_str_mv Zarate, Gabriela del Valle; Nader, Maria Elena Fatima; Viability and biological properties of probiotic vaginal lactobacilli after lyophilization and refrigerated storage into gelatin capsules; Elsevier; Process Biochemistry; 41; 8; 8-2006; 1779-1785
1359-5113
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2006.03.024
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S135951130600122X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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