Study of the stabilization process in Saccharomyces intra- and interspecific hybrids in fermentation conditions

Autores
Pérez Través, Laura; Lopes, Christian Ariel; Barrio, Eladio; Querol, Amparo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, we evaluated the genetic stabilization of artificialintra- (Saccharomyces cerevisiae) and interspecific (S. cerevisiae x Saccharomyceskudriavzevii) hybrids under wine fermentative conditions. Large-scale transitions in genome sizeandgenome reorganizationswere observedduring this process. Interspecific hybrids seem to need fewer generations to reach genetic stability than intraspecific hybrids.The largest number of molecular patterns recovered among the derived cloneswas observed for intraspecific hybrids, particularly for those obtained by rare-mating.Molecular markers analyses revealed that unstable clones can suffer changes during the industrial process to obtain active dry yeast. When no changes in molecular markers and ploidy were observed after this process, no changes ingenetic composition were confirmed by comparative genome hybridization, considering the clone as a stable hybrid. According to our results, under these conditions, fermentation steps 3 and 5 (30-50 generations)suffice to obtain genetically stable interspecific and intraspecific hybrids, respectively.
Fil: Pérez Través, Laura. Consejo Superior de Investigaciones Cientificas. Instituto de Agroquimica y Tecnologia de Alimentos; España. Universidad de Valencia; España
Fil: Lopes, Christian Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; España
Fil: Barrio, Eladio. Consejo Superior de Investigaciones Cientificas. Instituto de Agroquimica y Tecnologia de Alimentos; España. Universidad de Valencia; España
Fil: Querol, Amparo. Consejo Superior de Investigaciones Cientificas. Instituto de Agroquimica y Tecnologia de Alimentos; España
Materia
SACCHAROMYCES CEREVISIAE
SACCHAROMYCES KUDRIAVZEVII
RARE-MATING
MOLECULAR MARKERS
DNA CONTENT
STABILIZATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19685
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/19685
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Study of the stabilization process in Saccharomyces intra- and interspecific hybrids in fermentation conditionsPérez Través, LauraLopes, Christian ArielBarrio, EladioQuerol, AmparoSACCHAROMYCES CEREVISIAESACCHAROMYCES KUDRIAVZEVIIRARE-MATINGMOLECULAR MARKERSDNA CONTENTSTABILIZATIONhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4In this work, we evaluated the genetic stabilization of artificialintra- (Saccharomyces cerevisiae) and interspecific (S. cerevisiae x Saccharomyceskudriavzevii) hybrids under wine fermentative conditions. Large-scale transitions in genome sizeandgenome reorganizationswere observedduring this process. Interspecific hybrids seem to need fewer generations to reach genetic stability than intraspecific hybrids.The largest number of molecular patterns recovered among the derived cloneswas observed for intraspecific hybrids, particularly for those obtained by rare-mating.Molecular markers analyses revealed that unstable clones can suffer changes during the industrial process to obtain active dry yeast. When no changes in molecular markers and ploidy were observed after this process, no changes ingenetic composition were confirmed by comparative genome hybridization, considering the clone as a stable hybrid. According to our results, under these conditions, fermentation steps 3 and 5 (30-50 generations)suffice to obtain genetically stable interspecific and intraspecific hybrids, respectively.Fil: Pérez Través, Laura. Consejo Superior de Investigaciones Cientificas. Instituto de Agroquimica y Tecnologia de Alimentos; España. Universidad de Valencia; EspañaFil: Lopes, Christian Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; EspañaFil: Barrio, Eladio. Consejo Superior de Investigaciones Cientificas. Instituto de Agroquimica y Tecnologia de Alimentos; España. Universidad de Valencia; EspañaFil: Querol, Amparo. Consejo Superior de Investigaciones Cientificas. Instituto de Agroquimica y Tecnologia de Alimentos; EspañaViguera Editores2014-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19685Pérez Través, Laura; Lopes, Christian Ariel; Barrio, Eladio; Querol, Amparo; Study of the stabilization process in Saccharomyces intra- and interspecific hybrids in fermentation conditions; Viguera Editores; International Microbiology; 17; 2014; 1-12-2014; 213-2241139-67091618-1905CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://revistes.iec.cat/index.php/IM/article/viewFile/138514/137182info:eu-repo/semantics/altIdentifier/doi/10.2436/20.1501.01.224info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:20:40Zoai:ri.conicet.gov.ar:11336/19685instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:20:41.144CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Study of the stabilization process in Saccharomyces intra- and interspecific hybrids in fermentation conditions
title Study of the stabilization process in Saccharomyces intra- and interspecific hybrids in fermentation conditions
spellingShingle Study of the stabilization process in Saccharomyces intra- and interspecific hybrids in fermentation conditions
Pérez Través, Laura
SACCHAROMYCES CEREVISIAE
SACCHAROMYCES KUDRIAVZEVII
RARE-MATING
MOLECULAR MARKERS
DNA CONTENT
STABILIZATION
title_short Study of the stabilization process in Saccharomyces intra- and interspecific hybrids in fermentation conditions
title_full Study of the stabilization process in Saccharomyces intra- and interspecific hybrids in fermentation conditions
title_fullStr Study of the stabilization process in Saccharomyces intra- and interspecific hybrids in fermentation conditions
title_full_unstemmed Study of the stabilization process in Saccharomyces intra- and interspecific hybrids in fermentation conditions
title_sort Study of the stabilization process in Saccharomyces intra- and interspecific hybrids in fermentation conditions
dc.creator.none.fl_str_mv Pérez Través, Laura
Lopes, Christian Ariel
Barrio, Eladio
Querol, Amparo
author Pérez Través, Laura
author_facet Pérez Través, Laura
Lopes, Christian Ariel
Barrio, Eladio
Querol, Amparo
author_role author
author2 Lopes, Christian Ariel
Barrio, Eladio
Querol, Amparo
author2_role author
author
author
dc.subject.none.fl_str_mv SACCHAROMYCES CEREVISIAE
SACCHAROMYCES KUDRIAVZEVII
RARE-MATING
MOLECULAR MARKERS
DNA CONTENT
STABILIZATION
topic SACCHAROMYCES CEREVISIAE
SACCHAROMYCES KUDRIAVZEVII
RARE-MATING
MOLECULAR MARKERS
DNA CONTENT
STABILIZATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In this work, we evaluated the genetic stabilization of artificialintra- (Saccharomyces cerevisiae) and interspecific (S. cerevisiae x Saccharomyceskudriavzevii) hybrids under wine fermentative conditions. Large-scale transitions in genome sizeandgenome reorganizationswere observedduring this process. Interspecific hybrids seem to need fewer generations to reach genetic stability than intraspecific hybrids.The largest number of molecular patterns recovered among the derived cloneswas observed for intraspecific hybrids, particularly for those obtained by rare-mating.Molecular markers analyses revealed that unstable clones can suffer changes during the industrial process to obtain active dry yeast. When no changes in molecular markers and ploidy were observed after this process, no changes ingenetic composition were confirmed by comparative genome hybridization, considering the clone as a stable hybrid. According to our results, under these conditions, fermentation steps 3 and 5 (30-50 generations)suffice to obtain genetically stable interspecific and intraspecific hybrids, respectively.
Fil: Pérez Través, Laura. Consejo Superior de Investigaciones Cientificas. Instituto de Agroquimica y Tecnologia de Alimentos; España. Universidad de Valencia; España
Fil: Lopes, Christian Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; España
Fil: Barrio, Eladio. Consejo Superior de Investigaciones Cientificas. Instituto de Agroquimica y Tecnologia de Alimentos; España. Universidad de Valencia; España
Fil: Querol, Amparo. Consejo Superior de Investigaciones Cientificas. Instituto de Agroquimica y Tecnologia de Alimentos; España
description In this work, we evaluated the genetic stabilization of artificialintra- (Saccharomyces cerevisiae) and interspecific (S. cerevisiae x Saccharomyceskudriavzevii) hybrids under wine fermentative conditions. Large-scale transitions in genome sizeandgenome reorganizationswere observedduring this process. Interspecific hybrids seem to need fewer generations to reach genetic stability than intraspecific hybrids.The largest number of molecular patterns recovered among the derived cloneswas observed for intraspecific hybrids, particularly for those obtained by rare-mating.Molecular markers analyses revealed that unstable clones can suffer changes during the industrial process to obtain active dry yeast. When no changes in molecular markers and ploidy were observed after this process, no changes ingenetic composition were confirmed by comparative genome hybridization, considering the clone as a stable hybrid. According to our results, under these conditions, fermentation steps 3 and 5 (30-50 generations)suffice to obtain genetically stable interspecific and intraspecific hybrids, respectively.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/19685
Pérez Través, Laura; Lopes, Christian Ariel; Barrio, Eladio; Querol, Amparo; Study of the stabilization process in Saccharomyces intra- and interspecific hybrids in fermentation conditions; Viguera Editores; International Microbiology; 17; 2014; 1-12-2014; 213-224
1139-6709
1618-1905
CONICET Digital
CONICET
url http://hdl.handle.net/11336/19685
identifier_str_mv Pérez Través, Laura; Lopes, Christian Ariel; Barrio, Eladio; Querol, Amparo; Study of the stabilization process in Saccharomyces intra- and interspecific hybrids in fermentation conditions; Viguera Editores; International Microbiology; 17; 2014; 1-12-2014; 213-224
1139-6709
1618-1905
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://revistes.iec.cat/index.php/IM/article/viewFile/138514/137182
info:eu-repo/semantics/altIdentifier/doi/10.2436/20.1501.01.224
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Viguera Editores
publisher.none.fl_str_mv Viguera Editores
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082585494552576
score 13.22299

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