Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage

Autores
Meents, A.; Owen, R. L.; Murgida, Daniel Horacio; Hildebrandt, P.; Schneider, R.; Pradervand, C.; Bohler, P.; Schulze Briese, C.
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Radiation induced damage of metal centres in proteins is a severe problem in X-ray structure determination. Photoreduction can lead to erroneous structural implications, and in the worst cases cause structure solution to fail. Resonance Raman (RR) spectroscopy is well suited in-situ monitoring of X-ray induced photoreduction. However the laser excitation needed for RR can itself cause photoreduction of the metal centres. In the present study myoglobin and rubredoxin crystals were used as model systems to assess the feasibility of using RR for this application. It is shown that at least 10-15 RR spectra per crystal can be recorded at low laser power before severe photoreduction occurs.Furthermore it is possible to collect good quality RR spectra from cryocooled protein crystals with exposure times of only a few seconds. Following extended laser illumination photoreduction is observed through the formation and decay of spectral bands as a function of dose. The experimental setup planned for integration into the SLS protein crystallography beamlines is also described. This setup should also prove to be very useful for other experimental techniques at synchrotrons where X-ray photoreduction is a problem e.g. X-ray absorption spectroscopy.
Fil: Meents, A.. Swiss Light Source; Suiza
Fil: Owen, R. L.. Swiss Light Source; Suiza
Fil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Hildebrandt, P.. Swiss Light Source; Suiza
Fil: Schneider, R.. Swiss Light Source; Suiza
Fil: Pradervand, C.. Swiss Light Source; Suiza
Fil: Bohler, P.. Swiss Light Source; Suiza
Fil: Schulze Briese, C.. Swiss Light Source; Suiza
Materia
Raman microscopy
Radiation damage
Synchrotron radiation
In situ monitoring
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103362
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation DamageMeents, A.Owen, R. L.Murgida, Daniel HoracioHildebrandt, P.Schneider, R.Pradervand, C.Bohler, P.Schulze Briese, C.Raman microscopyRadiation damageSynchrotron radiationIn situ monitoringhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Radiation induced damage of metal centres in proteins is a severe problem in X-ray structure determination. Photoreduction can lead to erroneous structural implications, and in the worst cases cause structure solution to fail. Resonance Raman (RR) spectroscopy is well suited in-situ monitoring of X-ray induced photoreduction. However the laser excitation needed for RR can itself cause photoreduction of the metal centres. In the present study myoglobin and rubredoxin crystals were used as model systems to assess the feasibility of using RR for this application. It is shown that at least 10-15 RR spectra per crystal can be recorded at low laser power before severe photoreduction occurs.Furthermore it is possible to collect good quality RR spectra from cryocooled protein crystals with exposure times of only a few seconds. Following extended laser illumination photoreduction is observed through the formation and decay of spectral bands as a function of dose. The experimental setup planned for integration into the SLS protein crystallography beamlines is also described. This setup should also prove to be very useful for other experimental techniques at synchrotrons where X-ray photoreduction is a problem e.g. X-ray absorption spectroscopy.Fil: Meents, A.. Swiss Light Source; SuizaFil: Owen, R. L.. Swiss Light Source; SuizaFil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Hildebrandt, P.. Swiss Light Source; SuizaFil: Schneider, R.. Swiss Light Source; SuizaFil: Pradervand, C.. Swiss Light Source; SuizaFil: Bohler, P.. Swiss Light Source; SuizaFil: Schulze Briese, C.. Swiss Light Source; SuizaAmerican Institute of Physics2007-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103362Meents, A.; Owen, R. L.; Murgida, Daniel Horacio; Hildebrandt, P.; Schneider, R.; et al.; Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage; American Institute of Physics; AIP Conference Proceedings; 879; 12-2007; 1984-19880094-243XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://aip.scitation.org/doi/abs/10.1063/1.2436464info:eu-repo/semantics/altIdentifier/doi/10.1063/1.2436464info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:52:06Zoai:ri.conicet.gov.ar:11336/103362instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:52:06.794CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage
title Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage
spellingShingle Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage
Meents, A.
Raman microscopy
Radiation damage
Synchrotron radiation
In situ monitoring
title_short Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage
title_full Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage
title_fullStr Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage
title_full_unstemmed Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage
title_sort Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage
dc.creator.none.fl_str_mv Meents, A.
Owen, R. L.
Murgida, Daniel Horacio
Hildebrandt, P.
Schneider, R.
Pradervand, C.
Bohler, P.
Schulze Briese, C.
author Meents, A.
author_facet Meents, A.
Owen, R. L.
Murgida, Daniel Horacio
Hildebrandt, P.
Schneider, R.
Pradervand, C.
Bohler, P.
Schulze Briese, C.
author_role author
author2 Owen, R. L.
Murgida, Daniel Horacio
Hildebrandt, P.
Schneider, R.
Pradervand, C.
Bohler, P.
Schulze Briese, C.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Raman microscopy
Radiation damage
Synchrotron radiation
In situ monitoring
topic Raman microscopy
Radiation damage
Synchrotron radiation
In situ monitoring
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Radiation induced damage of metal centres in proteins is a severe problem in X-ray structure determination. Photoreduction can lead to erroneous structural implications, and in the worst cases cause structure solution to fail. Resonance Raman (RR) spectroscopy is well suited in-situ monitoring of X-ray induced photoreduction. However the laser excitation needed for RR can itself cause photoreduction of the metal centres. In the present study myoglobin and rubredoxin crystals were used as model systems to assess the feasibility of using RR for this application. It is shown that at least 10-15 RR spectra per crystal can be recorded at low laser power before severe photoreduction occurs.Furthermore it is possible to collect good quality RR spectra from cryocooled protein crystals with exposure times of only a few seconds. Following extended laser illumination photoreduction is observed through the formation and decay of spectral bands as a function of dose. The experimental setup planned for integration into the SLS protein crystallography beamlines is also described. This setup should also prove to be very useful for other experimental techniques at synchrotrons where X-ray photoreduction is a problem e.g. X-ray absorption spectroscopy.
Fil: Meents, A.. Swiss Light Source; Suiza
Fil: Owen, R. L.. Swiss Light Source; Suiza
Fil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Hildebrandt, P.. Swiss Light Source; Suiza
Fil: Schneider, R.. Swiss Light Source; Suiza
Fil: Pradervand, C.. Swiss Light Source; Suiza
Fil: Bohler, P.. Swiss Light Source; Suiza
Fil: Schulze Briese, C.. Swiss Light Source; Suiza
description Radiation induced damage of metal centres in proteins is a severe problem in X-ray structure determination. Photoreduction can lead to erroneous structural implications, and in the worst cases cause structure solution to fail. Resonance Raman (RR) spectroscopy is well suited in-situ monitoring of X-ray induced photoreduction. However the laser excitation needed for RR can itself cause photoreduction of the metal centres. In the present study myoglobin and rubredoxin crystals were used as model systems to assess the feasibility of using RR for this application. It is shown that at least 10-15 RR spectra per crystal can be recorded at low laser power before severe photoreduction occurs.Furthermore it is possible to collect good quality RR spectra from cryocooled protein crystals with exposure times of only a few seconds. Following extended laser illumination photoreduction is observed through the formation and decay of spectral bands as a function of dose. The experimental setup planned for integration into the SLS protein crystallography beamlines is also described. This setup should also prove to be very useful for other experimental techniques at synchrotrons where X-ray photoreduction is a problem e.g. X-ray absorption spectroscopy.
publishDate 2007
dc.date.none.fl_str_mv 2007-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103362
Meents, A.; Owen, R. L.; Murgida, Daniel Horacio; Hildebrandt, P.; Schneider, R.; et al.; Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage; American Institute of Physics; AIP Conference Proceedings; 879; 12-2007; 1984-1988
0094-243X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103362
identifier_str_mv Meents, A.; Owen, R. L.; Murgida, Daniel Horacio; Hildebrandt, P.; Schneider, R.; et al.; Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage; American Institute of Physics; AIP Conference Proceedings; 879; 12-2007; 1984-1988
0094-243X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://aip.scitation.org/doi/abs/10.1063/1.2436464
info:eu-repo/semantics/altIdentifier/doi/10.1063/1.2436464
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Institute of Physics
publisher.none.fl_str_mv American Institute of Physics
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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