Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage
- Autores
- Meents, A.; Owen, R. L.; Murgida, Daniel Horacio; Hildebrandt, P.; Schneider, R.; Pradervand, C.; Bohler, P.; Schulze Briese, C.
- Año de publicación
- 2007
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Radiation induced damage of metal centres in proteins is a severe problem in X-ray structure determination. Photoreduction can lead to erroneous structural implications, and in the worst cases cause structure solution to fail. Resonance Raman (RR) spectroscopy is well suited in-situ monitoring of X-ray induced photoreduction. However the laser excitation needed for RR can itself cause photoreduction of the metal centres. In the present study myoglobin and rubredoxin crystals were used as model systems to assess the feasibility of using RR for this application. It is shown that at least 10-15 RR spectra per crystal can be recorded at low laser power before severe photoreduction occurs.Furthermore it is possible to collect good quality RR spectra from cryocooled protein crystals with exposure times of only a few seconds. Following extended laser illumination photoreduction is observed through the formation and decay of spectral bands as a function of dose. The experimental setup planned for integration into the SLS protein crystallography beamlines is also described. This setup should also prove to be very useful for other experimental techniques at synchrotrons where X-ray photoreduction is a problem e.g. X-ray absorption spectroscopy.
Fil: Meents, A.. Swiss Light Source; Suiza
Fil: Owen, R. L.. Swiss Light Source; Suiza
Fil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Hildebrandt, P.. Swiss Light Source; Suiza
Fil: Schneider, R.. Swiss Light Source; Suiza
Fil: Pradervand, C.. Swiss Light Source; Suiza
Fil: Bohler, P.. Swiss Light Source; Suiza
Fil: Schulze Briese, C.. Swiss Light Source; Suiza - Materia
-
Raman microscopy
Radiation damage
Synchrotron radiation
In situ monitoring - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/103362
Ver los metadatos del registro completo
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Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation DamageMeents, A.Owen, R. L.Murgida, Daniel HoracioHildebrandt, P.Schneider, R.Pradervand, C.Bohler, P.Schulze Briese, C.Raman microscopyRadiation damageSynchrotron radiationIn situ monitoringhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Radiation induced damage of metal centres in proteins is a severe problem in X-ray structure determination. Photoreduction can lead to erroneous structural implications, and in the worst cases cause structure solution to fail. Resonance Raman (RR) spectroscopy is well suited in-situ monitoring of X-ray induced photoreduction. However the laser excitation needed for RR can itself cause photoreduction of the metal centres. In the present study myoglobin and rubredoxin crystals were used as model systems to assess the feasibility of using RR for this application. It is shown that at least 10-15 RR spectra per crystal can be recorded at low laser power before severe photoreduction occurs.Furthermore it is possible to collect good quality RR spectra from cryocooled protein crystals with exposure times of only a few seconds. Following extended laser illumination photoreduction is observed through the formation and decay of spectral bands as a function of dose. The experimental setup planned for integration into the SLS protein crystallography beamlines is also described. This setup should also prove to be very useful for other experimental techniques at synchrotrons where X-ray photoreduction is a problem e.g. X-ray absorption spectroscopy.Fil: Meents, A.. Swiss Light Source; SuizaFil: Owen, R. L.. Swiss Light Source; SuizaFil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Hildebrandt, P.. Swiss Light Source; SuizaFil: Schneider, R.. Swiss Light Source; SuizaFil: Pradervand, C.. Swiss Light Source; SuizaFil: Bohler, P.. Swiss Light Source; SuizaFil: Schulze Briese, C.. Swiss Light Source; SuizaAmerican Institute of Physics2007-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103362Meents, A.; Owen, R. L.; Murgida, Daniel Horacio; Hildebrandt, P.; Schneider, R.; et al.; Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage; American Institute of Physics; AIP Conference Proceedings; 879; 12-2007; 1984-19880094-243XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://aip.scitation.org/doi/abs/10.1063/1.2436464info:eu-repo/semantics/altIdentifier/doi/10.1063/1.2436464info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:23Zoai:ri.conicet.gov.ar:11336/103362instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:24.125CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage |
title |
Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage |
spellingShingle |
Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage Meents, A. Raman microscopy Radiation damage Synchrotron radiation In situ monitoring |
title_short |
Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage |
title_full |
Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage |
title_fullStr |
Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage |
title_full_unstemmed |
Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage |
title_sort |
Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage |
dc.creator.none.fl_str_mv |
Meents, A. Owen, R. L. Murgida, Daniel Horacio Hildebrandt, P. Schneider, R. Pradervand, C. Bohler, P. Schulze Briese, C. |
author |
Meents, A. |
author_facet |
Meents, A. Owen, R. L. Murgida, Daniel Horacio Hildebrandt, P. Schneider, R. Pradervand, C. Bohler, P. Schulze Briese, C. |
author_role |
author |
author2 |
Owen, R. L. Murgida, Daniel Horacio Hildebrandt, P. Schneider, R. Pradervand, C. Bohler, P. Schulze Briese, C. |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Raman microscopy Radiation damage Synchrotron radiation In situ monitoring |
topic |
Raman microscopy Radiation damage Synchrotron radiation In situ monitoring |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Radiation induced damage of metal centres in proteins is a severe problem in X-ray structure determination. Photoreduction can lead to erroneous structural implications, and in the worst cases cause structure solution to fail. Resonance Raman (RR) spectroscopy is well suited in-situ monitoring of X-ray induced photoreduction. However the laser excitation needed for RR can itself cause photoreduction of the metal centres. In the present study myoglobin and rubredoxin crystals were used as model systems to assess the feasibility of using RR for this application. It is shown that at least 10-15 RR spectra per crystal can be recorded at low laser power before severe photoreduction occurs.Furthermore it is possible to collect good quality RR spectra from cryocooled protein crystals with exposure times of only a few seconds. Following extended laser illumination photoreduction is observed through the formation and decay of spectral bands as a function of dose. The experimental setup planned for integration into the SLS protein crystallography beamlines is also described. This setup should also prove to be very useful for other experimental techniques at synchrotrons where X-ray photoreduction is a problem e.g. X-ray absorption spectroscopy. Fil: Meents, A.. Swiss Light Source; Suiza Fil: Owen, R. L.. Swiss Light Source; Suiza Fil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina Fil: Hildebrandt, P.. Swiss Light Source; Suiza Fil: Schneider, R.. Swiss Light Source; Suiza Fil: Pradervand, C.. Swiss Light Source; Suiza Fil: Bohler, P.. Swiss Light Source; Suiza Fil: Schulze Briese, C.. Swiss Light Source; Suiza |
description |
Radiation induced damage of metal centres in proteins is a severe problem in X-ray structure determination. Photoreduction can lead to erroneous structural implications, and in the worst cases cause structure solution to fail. Resonance Raman (RR) spectroscopy is well suited in-situ monitoring of X-ray induced photoreduction. However the laser excitation needed for RR can itself cause photoreduction of the metal centres. In the present study myoglobin and rubredoxin crystals were used as model systems to assess the feasibility of using RR for this application. It is shown that at least 10-15 RR spectra per crystal can be recorded at low laser power before severe photoreduction occurs.Furthermore it is possible to collect good quality RR spectra from cryocooled protein crystals with exposure times of only a few seconds. Following extended laser illumination photoreduction is observed through the formation and decay of spectral bands as a function of dose. The experimental setup planned for integration into the SLS protein crystallography beamlines is also described. This setup should also prove to be very useful for other experimental techniques at synchrotrons where X-ray photoreduction is a problem e.g. X-ray absorption spectroscopy. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/103362 Meents, A.; Owen, R. L.; Murgida, Daniel Horacio; Hildebrandt, P.; Schneider, R.; et al.; Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage; American Institute of Physics; AIP Conference Proceedings; 879; 12-2007; 1984-1988 0094-243X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/103362 |
identifier_str_mv |
Meents, A.; Owen, R. L.; Murgida, Daniel Horacio; Hildebrandt, P.; Schneider, R.; et al.; Resonance Raman Spectroscopy For In-Situ Monitoring Of Radiation Damage; American Institute of Physics; AIP Conference Proceedings; 879; 12-2007; 1984-1988 0094-243X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://aip.scitation.org/doi/abs/10.1063/1.2436464 info:eu-repo/semantics/altIdentifier/doi/10.1063/1.2436464 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Institute of Physics |
publisher.none.fl_str_mv |
American Institute of Physics |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |