Fluctuating temperatures terminate dormancy in Cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling

Autores
Huarte, H. Roberto; Luna, Maria Virginia; Pagano, Eduardo A.; Zavala, Jorge Alberto; Benech-Arnold, Roberto Luis
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fluctuating temperatures terminate seed dormancy in many species, including Cynara cardunculus (L.) var. sylvestris. Termination of physiological dormancy requires low ratios of abscisic acid (ABA)/gibberellins (GA). In a previous paper we have shown that physiological responses to fluctuating temperatures comprise a reduction of abscisic acid (ABA) content and sensitivity. However, a possible stimulation of GA synthesis was also suggested as part of the mechanism. That possible stimulation, as well as the identification of potential regulatory sites for ABA and GA metabolism and signalling involved in the termination of dormancy by fluctuating temperatures, are yet to be determined. In this study, we measured GA content and sensitivity in seeds incubated under constant and fluctuating temperatures. We also assessed the expression of several genes involved in ABA and GA metabolism and signalling. Our results show that fluctuating temperatures reduce ABA/GA ratios through a reduction in ABA accumulation during incubation but without altering GA synthesis as compared to that observed under constant temperatures. On the other hand, fluctuating temperatures did not increase sensitivity to GA. Fluctuating temperatures reduced the expression of CycaNCED and CycaABI5 (ABA synthesis and signalling genes) with a temporal pattern that coincides with the interruption of ABA accumulation that precedes germination of seeds incubated under fluctuating temperatures. However, fluctuating temperatures did not modify the expression of CycaCYP707A2 (ABA inactivation) as compared to that observed under constant temperatures. Consistent with our determinations of GA content and sensitivity, fluctuating temperatures did not modify the expression of GA synthesis (CycaGA3ox) and signalling genes (CycaRGL2 and CycaGAI) in relation to that observed at constant temperatures. These results show that fluctuating temperatures terminate dormancy in Cynara cardunculus seeds through an interruption in ABA accumulation and a reduction in ABA signalling exerted at the level of CycaNCED and CycaABI5 expression.
Fil: Huarte, H. Roberto. Pontificia Universidad Católica Argentina "Santa María de Los Buenos Aires"; Argentina
Fil: Luna, Maria Virginia. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Catedra de Fisiologia Vegetal; Argentina
Fil: Pagano, Eduardo A.. Universidad de Buenos Aires. Facultad de Agronomia; Argentina
Fil: Zavala, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Benech-Arnold, Roberto Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Materia
Abscisic Acid - Dormancy
Fluctuating Temperatures
Gene Expression
Gibberellins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7519
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/7519
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fluctuating temperatures terminate dormancy in Cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signallingHuarte, H. RobertoLuna, Maria VirginiaPagano, Eduardo A.Zavala, Jorge AlbertoBenech-Arnold, Roberto LuisAbscisic Acid - DormancyFluctuating TemperaturesGene ExpressionGibberellinshttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Fluctuating temperatures terminate seed dormancy in many species, including Cynara cardunculus (L.) var. sylvestris. Termination of physiological dormancy requires low ratios of abscisic acid (ABA)/gibberellins (GA). In a previous paper we have shown that physiological responses to fluctuating temperatures comprise a reduction of abscisic acid (ABA) content and sensitivity. However, a possible stimulation of GA synthesis was also suggested as part of the mechanism. That possible stimulation, as well as the identification of potential regulatory sites for ABA and GA metabolism and signalling involved in the termination of dormancy by fluctuating temperatures, are yet to be determined. In this study, we measured GA content and sensitivity in seeds incubated under constant and fluctuating temperatures. We also assessed the expression of several genes involved in ABA and GA metabolism and signalling. Our results show that fluctuating temperatures reduce ABA/GA ratios through a reduction in ABA accumulation during incubation but without altering GA synthesis as compared to that observed under constant temperatures. On the other hand, fluctuating temperatures did not increase sensitivity to GA. Fluctuating temperatures reduced the expression of CycaNCED and CycaABI5 (ABA synthesis and signalling genes) with a temporal pattern that coincides with the interruption of ABA accumulation that precedes germination of seeds incubated under fluctuating temperatures. However, fluctuating temperatures did not modify the expression of CycaCYP707A2 (ABA inactivation) as compared to that observed under constant temperatures. Consistent with our determinations of GA content and sensitivity, fluctuating temperatures did not modify the expression of GA synthesis (CycaGA3ox) and signalling genes (CycaRGL2 and CycaGAI) in relation to that observed at constant temperatures. These results show that fluctuating temperatures terminate dormancy in Cynara cardunculus seeds through an interruption in ABA accumulation and a reduction in ABA signalling exerted at the level of CycaNCED and CycaABI5 expression.Fil: Huarte, H. Roberto. Pontificia Universidad Católica Argentina "Santa María de Los Buenos Aires"; ArgentinaFil: Luna, Maria Virginia. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Catedra de Fisiologia Vegetal; ArgentinaFil: Pagano, Eduardo A.. Universidad de Buenos Aires. Facultad de Agronomia; ArgentinaFil: Zavala, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Benech-Arnold, Roberto Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaCambridge University Press2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7519Huarte, H. Roberto; Luna, Maria Virginia; Pagano, Eduardo A.; Zavala, Jorge Alberto; Benech-Arnold, Roberto Luis; Fluctuating temperatures terminate dormancy in Cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling; Cambridge University Press; Seed Science Research; 24; 2; 4-2014; 79-890960-2585enginfo:eu-repo/semantics/altIdentifier/url/http://goo.gl/4Z9R0hinfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0960258514000051info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:15Zoai:ri.conicet.gov.ar:11336/7519instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:15.419CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fluctuating temperatures terminate dormancy in Cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling
title Fluctuating temperatures terminate dormancy in Cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling
spellingShingle Fluctuating temperatures terminate dormancy in Cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling
Huarte, H. Roberto
Abscisic Acid - Dormancy
Fluctuating Temperatures
Gene Expression
Gibberellins
title_short Fluctuating temperatures terminate dormancy in Cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling
title_full Fluctuating temperatures terminate dormancy in Cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling
title_fullStr Fluctuating temperatures terminate dormancy in Cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling
title_full_unstemmed Fluctuating temperatures terminate dormancy in Cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling
title_sort Fluctuating temperatures terminate dormancy in Cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling
dc.creator.none.fl_str_mv Huarte, H. Roberto
Luna, Maria Virginia
Pagano, Eduardo A.
Zavala, Jorge Alberto
Benech-Arnold, Roberto Luis
author Huarte, H. Roberto
author_facet Huarte, H. Roberto
Luna, Maria Virginia
Pagano, Eduardo A.
Zavala, Jorge Alberto
Benech-Arnold, Roberto Luis
author_role author
author2 Luna, Maria Virginia
Pagano, Eduardo A.
Zavala, Jorge Alberto
Benech-Arnold, Roberto Luis
author2_role author
author
author
author
dc.subject.none.fl_str_mv Abscisic Acid - Dormancy
Fluctuating Temperatures
Gene Expression
Gibberellins
topic Abscisic Acid - Dormancy
Fluctuating Temperatures
Gene Expression
Gibberellins
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Fluctuating temperatures terminate seed dormancy in many species, including Cynara cardunculus (L.) var. sylvestris. Termination of physiological dormancy requires low ratios of abscisic acid (ABA)/gibberellins (GA). In a previous paper we have shown that physiological responses to fluctuating temperatures comprise a reduction of abscisic acid (ABA) content and sensitivity. However, a possible stimulation of GA synthesis was also suggested as part of the mechanism. That possible stimulation, as well as the identification of potential regulatory sites for ABA and GA metabolism and signalling involved in the termination of dormancy by fluctuating temperatures, are yet to be determined. In this study, we measured GA content and sensitivity in seeds incubated under constant and fluctuating temperatures. We also assessed the expression of several genes involved in ABA and GA metabolism and signalling. Our results show that fluctuating temperatures reduce ABA/GA ratios through a reduction in ABA accumulation during incubation but without altering GA synthesis as compared to that observed under constant temperatures. On the other hand, fluctuating temperatures did not increase sensitivity to GA. Fluctuating temperatures reduced the expression of CycaNCED and CycaABI5 (ABA synthesis and signalling genes) with a temporal pattern that coincides with the interruption of ABA accumulation that precedes germination of seeds incubated under fluctuating temperatures. However, fluctuating temperatures did not modify the expression of CycaCYP707A2 (ABA inactivation) as compared to that observed under constant temperatures. Consistent with our determinations of GA content and sensitivity, fluctuating temperatures did not modify the expression of GA synthesis (CycaGA3ox) and signalling genes (CycaRGL2 and CycaGAI) in relation to that observed at constant temperatures. These results show that fluctuating temperatures terminate dormancy in Cynara cardunculus seeds through an interruption in ABA accumulation and a reduction in ABA signalling exerted at the level of CycaNCED and CycaABI5 expression.
Fil: Huarte, H. Roberto. Pontificia Universidad Católica Argentina "Santa María de Los Buenos Aires"; Argentina
Fil: Luna, Maria Virginia. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Catedra de Fisiologia Vegetal; Argentina
Fil: Pagano, Eduardo A.. Universidad de Buenos Aires. Facultad de Agronomia; Argentina
Fil: Zavala, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Benech-Arnold, Roberto Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
description Fluctuating temperatures terminate seed dormancy in many species, including Cynara cardunculus (L.) var. sylvestris. Termination of physiological dormancy requires low ratios of abscisic acid (ABA)/gibberellins (GA). In a previous paper we have shown that physiological responses to fluctuating temperatures comprise a reduction of abscisic acid (ABA) content and sensitivity. However, a possible stimulation of GA synthesis was also suggested as part of the mechanism. That possible stimulation, as well as the identification of potential regulatory sites for ABA and GA metabolism and signalling involved in the termination of dormancy by fluctuating temperatures, are yet to be determined. In this study, we measured GA content and sensitivity in seeds incubated under constant and fluctuating temperatures. We also assessed the expression of several genes involved in ABA and GA metabolism and signalling. Our results show that fluctuating temperatures reduce ABA/GA ratios through a reduction in ABA accumulation during incubation but without altering GA synthesis as compared to that observed under constant temperatures. On the other hand, fluctuating temperatures did not increase sensitivity to GA. Fluctuating temperatures reduced the expression of CycaNCED and CycaABI5 (ABA synthesis and signalling genes) with a temporal pattern that coincides with the interruption of ABA accumulation that precedes germination of seeds incubated under fluctuating temperatures. However, fluctuating temperatures did not modify the expression of CycaCYP707A2 (ABA inactivation) as compared to that observed under constant temperatures. Consistent with our determinations of GA content and sensitivity, fluctuating temperatures did not modify the expression of GA synthesis (CycaGA3ox) and signalling genes (CycaRGL2 and CycaGAI) in relation to that observed at constant temperatures. These results show that fluctuating temperatures terminate dormancy in Cynara cardunculus seeds through an interruption in ABA accumulation and a reduction in ABA signalling exerted at the level of CycaNCED and CycaABI5 expression.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7519
Huarte, H. Roberto; Luna, Maria Virginia; Pagano, Eduardo A.; Zavala, Jorge Alberto; Benech-Arnold, Roberto Luis; Fluctuating temperatures terminate dormancy in Cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling; Cambridge University Press; Seed Science Research; 24; 2; 4-2014; 79-89
0960-2585
url http://hdl.handle.net/11336/7519
identifier_str_mv Huarte, H. Roberto; Luna, Maria Virginia; Pagano, Eduardo A.; Zavala, Jorge Alberto; Benech-Arnold, Roberto Luis; Fluctuating temperatures terminate dormancy in Cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling; Cambridge University Press; Seed Science Research; 24; 2; 4-2014; 79-89
0960-2585
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://goo.gl/4Z9R0h
info:eu-repo/semantics/altIdentifier/doi/10.1017/S0960258514000051
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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