Uncovering Photolyase/Cryptochrome Genes Diversity in Aquatic Microbiomes Exposed to Diverse UV-B Regimes

Autores
Alonso Reyes, Daniel Gonzalo; Farias, Maria Eugenia; Albarracín, Virginia Helena
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
During evolution, microorganisms exposed to high UV-B doses developed a fine-tuned photo-enzymes called ?photolyases? to cope with DNA damage by UV-B. These photoreceptors belonging to the Cryptochrome/Photolyase Family (CPF) were well characterized at the genomic and proteomic level in bacteria isolated from a wide range of environments. In this work, we go further towards studying the abundance of CPF on aquatic microbial communities from different geographic regions across the globe. Metagenomics data combined with geo-referenced solar irradiation measurements indicated that the higher the UV-B dose suffered by the microbiome´s environment, the higher the abundance of CPF genes and lower the microbial diversity. A connection between CPF abundance and radiation intensity/photoperiod was reported. Likewise, cryptochrome-like genes were found abundant in most exposed microbiomes, indicating a complementary role to standard photolyases. Also, we observed that CPFs are more likely present in dominant taxa of the highly irradiated microbiomes, suggesting an evolutionary force for survival and dominance under extreme solar exposure. Finally, this work reported three novel CPF clades not identified so far, proving the potential of global metagenomic analyses in detecting novel proteins.
Fil: Alonso Reyes, Daniel Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
UV
extremophile
metagenomic analyses
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/140495
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Uncovering Photolyase/Cryptochrome Genes Diversity in Aquatic Microbiomes Exposed to Diverse UV-B RegimesAlonso Reyes, Daniel GonzaloFarias, Maria EugeniaAlbarracín, Virginia HelenaUVextremophilemetagenomic analyseshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1During evolution, microorganisms exposed to high UV-B doses developed a fine-tuned photo-enzymes called ?photolyases? to cope with DNA damage by UV-B. These photoreceptors belonging to the Cryptochrome/Photolyase Family (CPF) were well characterized at the genomic and proteomic level in bacteria isolated from a wide range of environments. In this work, we go further towards studying the abundance of CPF on aquatic microbial communities from different geographic regions across the globe. Metagenomics data combined with geo-referenced solar irradiation measurements indicated that the higher the UV-B dose suffered by the microbiome´s environment, the higher the abundance of CPF genes and lower the microbial diversity. A connection between CPF abundance and radiation intensity/photoperiod was reported. Likewise, cryptochrome-like genes were found abundant in most exposed microbiomes, indicating a complementary role to standard photolyases. Also, we observed that CPFs are more likely present in dominant taxa of the highly irradiated microbiomes, suggesting an evolutionary force for survival and dominance under extreme solar exposure. Finally, this work reported three novel CPF clades not identified so far, proving the potential of global metagenomic analyses in detecting novel proteins.Fil: Alonso Reyes, Daniel Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaInter-Research2020-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/140495Alonso Reyes, Daniel Gonzalo; Farias, Maria Eugenia; Albarracín, Virginia Helena; Uncovering Photolyase/Cryptochrome Genes Diversity in Aquatic Microbiomes Exposed to Diverse UV-B Regimes; Inter-Research; Aquatic Microbial Ecology; 85; 1; 9-2020; 141-1540948-3055CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.int-res.com/prepress/a01947.htmlinfo:eu-repo/semantics/altIdentifier/doi/10.3354/ame01947info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:27Zoai:ri.conicet.gov.ar:11336/140495instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:27.376CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Uncovering Photolyase/Cryptochrome Genes Diversity in Aquatic Microbiomes Exposed to Diverse UV-B Regimes
title Uncovering Photolyase/Cryptochrome Genes Diversity in Aquatic Microbiomes Exposed to Diverse UV-B Regimes
spellingShingle Uncovering Photolyase/Cryptochrome Genes Diversity in Aquatic Microbiomes Exposed to Diverse UV-B Regimes
Alonso Reyes, Daniel Gonzalo
UV
extremophile
metagenomic analyses
title_short Uncovering Photolyase/Cryptochrome Genes Diversity in Aquatic Microbiomes Exposed to Diverse UV-B Regimes
title_full Uncovering Photolyase/Cryptochrome Genes Diversity in Aquatic Microbiomes Exposed to Diverse UV-B Regimes
title_fullStr Uncovering Photolyase/Cryptochrome Genes Diversity in Aquatic Microbiomes Exposed to Diverse UV-B Regimes
title_full_unstemmed Uncovering Photolyase/Cryptochrome Genes Diversity in Aquatic Microbiomes Exposed to Diverse UV-B Regimes
title_sort Uncovering Photolyase/Cryptochrome Genes Diversity in Aquatic Microbiomes Exposed to Diverse UV-B Regimes
dc.creator.none.fl_str_mv Alonso Reyes, Daniel Gonzalo
Farias, Maria Eugenia
Albarracín, Virginia Helena
author Alonso Reyes, Daniel Gonzalo
author_facet Alonso Reyes, Daniel Gonzalo
Farias, Maria Eugenia
Albarracín, Virginia Helena
author_role author
author2 Farias, Maria Eugenia
Albarracín, Virginia Helena
author2_role author
author
dc.subject.none.fl_str_mv UV
extremophile
metagenomic analyses
topic UV
extremophile
metagenomic analyses
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv During evolution, microorganisms exposed to high UV-B doses developed a fine-tuned photo-enzymes called ?photolyases? to cope with DNA damage by UV-B. These photoreceptors belonging to the Cryptochrome/Photolyase Family (CPF) were well characterized at the genomic and proteomic level in bacteria isolated from a wide range of environments. In this work, we go further towards studying the abundance of CPF on aquatic microbial communities from different geographic regions across the globe. Metagenomics data combined with geo-referenced solar irradiation measurements indicated that the higher the UV-B dose suffered by the microbiome´s environment, the higher the abundance of CPF genes and lower the microbial diversity. A connection between CPF abundance and radiation intensity/photoperiod was reported. Likewise, cryptochrome-like genes were found abundant in most exposed microbiomes, indicating a complementary role to standard photolyases. Also, we observed that CPFs are more likely present in dominant taxa of the highly irradiated microbiomes, suggesting an evolutionary force for survival and dominance under extreme solar exposure. Finally, this work reported three novel CPF clades not identified so far, proving the potential of global metagenomic analyses in detecting novel proteins.
Fil: Alonso Reyes, Daniel Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Albarracín, Virginia Helena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description During evolution, microorganisms exposed to high UV-B doses developed a fine-tuned photo-enzymes called ?photolyases? to cope with DNA damage by UV-B. These photoreceptors belonging to the Cryptochrome/Photolyase Family (CPF) were well characterized at the genomic and proteomic level in bacteria isolated from a wide range of environments. In this work, we go further towards studying the abundance of CPF on aquatic microbial communities from different geographic regions across the globe. Metagenomics data combined with geo-referenced solar irradiation measurements indicated that the higher the UV-B dose suffered by the microbiome´s environment, the higher the abundance of CPF genes and lower the microbial diversity. A connection between CPF abundance and radiation intensity/photoperiod was reported. Likewise, cryptochrome-like genes were found abundant in most exposed microbiomes, indicating a complementary role to standard photolyases. Also, we observed that CPFs are more likely present in dominant taxa of the highly irradiated microbiomes, suggesting an evolutionary force for survival and dominance under extreme solar exposure. Finally, this work reported three novel CPF clades not identified so far, proving the potential of global metagenomic analyses in detecting novel proteins.
publishDate 2020
dc.date.none.fl_str_mv 2020-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/140495
Alonso Reyes, Daniel Gonzalo; Farias, Maria Eugenia; Albarracín, Virginia Helena; Uncovering Photolyase/Cryptochrome Genes Diversity in Aquatic Microbiomes Exposed to Diverse UV-B Regimes; Inter-Research; Aquatic Microbial Ecology; 85; 1; 9-2020; 141-154
0948-3055
CONICET Digital
CONICET
url http://hdl.handle.net/11336/140495
identifier_str_mv Alonso Reyes, Daniel Gonzalo; Farias, Maria Eugenia; Albarracín, Virginia Helena; Uncovering Photolyase/Cryptochrome Genes Diversity in Aquatic Microbiomes Exposed to Diverse UV-B Regimes; Inter-Research; Aquatic Microbial Ecology; 85; 1; 9-2020; 141-154
0948-3055
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.int-res.com/prepress/a01947.html
info:eu-repo/semantics/altIdentifier/doi/10.3354/ame01947
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Inter-Research
publisher.none.fl_str_mv Inter-Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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