The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells

Autores
Guarniolo, D; Caballero, P. A.; Chapana, A.; Sosa, E.; Fernández, R. A.; Sosa, M. A.; Carvelli, Flavia Lorena
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Botulism is a neuroparalitic disease caused by botulinum neurotoxins (NTBo, serotypes A-G) produced by Clostridium botulinum, whose main reservoir is the soil (Su). Infant botulism is a toxiinfection, caused by ingestion of spores, subsequent colonization and production of toxins in situ. The autochthonous NTBo would correspond to subtype A2, and have higher toxicity than A1 (Botox®), so in the future, they could be used as a therapeutic agent. The NTBos mechanism of action on certain pathologies is still to be clarified. Previous results from our laboratory showed that autochthonous NTBo 1935 from Su, degrades actin of rat brain homogenates, suggesting this protein could be an active target of NTBos. In this work, the action of this NTBo on the actin cytoskeleton in mammary tumor cells was evaluated. The NTBos of Su from strain 1935 and strain A Hall (both serotype A) were purified by saline precipitation. MCF7 cells (breast cancer cells) were cultured in petri dishes or coverslips with 250 LD50 of the NTBos for 25, 45 or 90 min. After incubations, cells were processed for western blot or immunofluorescence in order to evaluate the distribution and expression of actin. NTBo 1935 produced a higher actin degradation and an increased location of this protein at plasma membrane in comparison with A Hall in dependent-time manner. However, at 90 min of treatment, we observed 90% of cytotoxicity and further studies at this time were not evaluated. These results provide new insights about the NTBo mechanism of action and its possible use in the fight against breast cancer.
Fil: Guarniolo, D. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Caballero, P. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Chapana, A.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sosa, E.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Fernández, R. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Sosa, M. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Carvelli, Flavia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
Materia
BOTULINUM NEUROTOXIN
BREAST CANCER
ACTIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/210158
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cellsGuarniolo, DCaballero, P. A.Chapana, A.Sosa, E.Fernández, R. A.Sosa, M. A.Carvelli, Flavia LorenaBOTULINUM NEUROTOXINBREAST CANCERACTINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Botulism is a neuroparalitic disease caused by botulinum neurotoxins (NTBo, serotypes A-G) produced by Clostridium botulinum, whose main reservoir is the soil (Su). Infant botulism is a toxiinfection, caused by ingestion of spores, subsequent colonization and production of toxins in situ. The autochthonous NTBo would correspond to subtype A2, and have higher toxicity than A1 (Botox®), so in the future, they could be used as a therapeutic agent. The NTBos mechanism of action on certain pathologies is still to be clarified. Previous results from our laboratory showed that autochthonous NTBo 1935 from Su, degrades actin of rat brain homogenates, suggesting this protein could be an active target of NTBos. In this work, the action of this NTBo on the actin cytoskeleton in mammary tumor cells was evaluated. The NTBos of Su from strain 1935 and strain A Hall (both serotype A) were purified by saline precipitation. MCF7 cells (breast cancer cells) were cultured in petri dishes or coverslips with 250 LD50 of the NTBos for 25, 45 or 90 min. After incubations, cells were processed for western blot or immunofluorescence in order to evaluate the distribution and expression of actin. NTBo 1935 produced a higher actin degradation and an increased location of this protein at plasma membrane in comparison with A Hall in dependent-time manner. However, at 90 min of treatment, we observed 90% of cytotoxicity and further studies at this time were not evaluated. These results provide new insights about the NTBo mechanism of action and its possible use in the fight against breast cancer.Fil: Guarniolo, D. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Caballero, P. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Chapana, A.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Sosa, E.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Fernández, R. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Sosa, M. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Carvelli, Flavia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaXXXVII Reunión Científica Anual de la Sociedad de Biología de CuyoSan LuisArgentinaSociedad de Biología de CuyoTech Science Press2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/210158The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 14-150327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v44nSuppl.1/39488Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:07:18Zoai:ri.conicet.gov.ar:11336/210158instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:07:19.239CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells
title The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells
spellingShingle The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells
Guarniolo, D
BOTULINUM NEUROTOXIN
BREAST CANCER
ACTIN
title_short The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells
title_full The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells
title_fullStr The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells
title_full_unstemmed The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells
title_sort The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells
dc.creator.none.fl_str_mv Guarniolo, D
Caballero, P. A.
Chapana, A.
Sosa, E.
Fernández, R. A.
Sosa, M. A.
Carvelli, Flavia Lorena
author Guarniolo, D
author_facet Guarniolo, D
Caballero, P. A.
Chapana, A.
Sosa, E.
Fernández, R. A.
Sosa, M. A.
Carvelli, Flavia Lorena
author_role author
author2 Caballero, P. A.
Chapana, A.
Sosa, E.
Fernández, R. A.
Sosa, M. A.
Carvelli, Flavia Lorena
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BOTULINUM NEUROTOXIN
BREAST CANCER
ACTIN
topic BOTULINUM NEUROTOXIN
BREAST CANCER
ACTIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Botulism is a neuroparalitic disease caused by botulinum neurotoxins (NTBo, serotypes A-G) produced by Clostridium botulinum, whose main reservoir is the soil (Su). Infant botulism is a toxiinfection, caused by ingestion of spores, subsequent colonization and production of toxins in situ. The autochthonous NTBo would correspond to subtype A2, and have higher toxicity than A1 (Botox®), so in the future, they could be used as a therapeutic agent. The NTBos mechanism of action on certain pathologies is still to be clarified. Previous results from our laboratory showed that autochthonous NTBo 1935 from Su, degrades actin of rat brain homogenates, suggesting this protein could be an active target of NTBos. In this work, the action of this NTBo on the actin cytoskeleton in mammary tumor cells was evaluated. The NTBos of Su from strain 1935 and strain A Hall (both serotype A) were purified by saline precipitation. MCF7 cells (breast cancer cells) were cultured in petri dishes or coverslips with 250 LD50 of the NTBos for 25, 45 or 90 min. After incubations, cells were processed for western blot or immunofluorescence in order to evaluate the distribution and expression of actin. NTBo 1935 produced a higher actin degradation and an increased location of this protein at plasma membrane in comparison with A Hall in dependent-time manner. However, at 90 min of treatment, we observed 90% of cytotoxicity and further studies at this time were not evaluated. These results provide new insights about the NTBo mechanism of action and its possible use in the fight against breast cancer.
Fil: Guarniolo, D. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Caballero, P. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Chapana, A.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sosa, E.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Fernández, R. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Sosa, M. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Carvelli, Flavia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
description Botulism is a neuroparalitic disease caused by botulinum neurotoxins (NTBo, serotypes A-G) produced by Clostridium botulinum, whose main reservoir is the soil (Su). Infant botulism is a toxiinfection, caused by ingestion of spores, subsequent colonization and production of toxins in situ. The autochthonous NTBo would correspond to subtype A2, and have higher toxicity than A1 (Botox®), so in the future, they could be used as a therapeutic agent. The NTBos mechanism of action on certain pathologies is still to be clarified. Previous results from our laboratory showed that autochthonous NTBo 1935 from Su, degrades actin of rat brain homogenates, suggesting this protein could be an active target of NTBos. In this work, the action of this NTBo on the actin cytoskeleton in mammary tumor cells was evaluated. The NTBos of Su from strain 1935 and strain A Hall (both serotype A) were purified by saline precipitation. MCF7 cells (breast cancer cells) were cultured in petri dishes or coverslips with 250 LD50 of the NTBos for 25, 45 or 90 min. After incubations, cells were processed for western blot or immunofluorescence in order to evaluate the distribution and expression of actin. NTBo 1935 produced a higher actin degradation and an increased location of this protein at plasma membrane in comparison with A Hall in dependent-time manner. However, at 90 min of treatment, we observed 90% of cytotoxicity and further studies at this time were not evaluated. These results provide new insights about the NTBo mechanism of action and its possible use in the fight against breast cancer.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/210158
The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 14-15
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/210158
identifier_str_mv The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 14-15
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v44nSuppl.1/39488
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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