The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells
- Autores
- Guarniolo, D; Caballero, P. A.; Chapana, A.; Sosa, E.; Fernández, R. A.; Sosa, M. A.; Carvelli, Flavia Lorena
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Botulism is a neuroparalitic disease caused by botulinum neurotoxins (NTBo, serotypes A-G) produced by Clostridium botulinum, whose main reservoir is the soil (Su). Infant botulism is a toxiinfection, caused by ingestion of spores, subsequent colonization and production of toxins in situ. The autochthonous NTBo would correspond to subtype A2, and have higher toxicity than A1 (Botox®), so in the future, they could be used as a therapeutic agent. The NTBos mechanism of action on certain pathologies is still to be clarified. Previous results from our laboratory showed that autochthonous NTBo 1935 from Su, degrades actin of rat brain homogenates, suggesting this protein could be an active target of NTBos. In this work, the action of this NTBo on the actin cytoskeleton in mammary tumor cells was evaluated. The NTBos of Su from strain 1935 and strain A Hall (both serotype A) were purified by saline precipitation. MCF7 cells (breast cancer cells) were cultured in petri dishes or coverslips with 250 LD50 of the NTBos for 25, 45 or 90 min. After incubations, cells were processed for western blot or immunofluorescence in order to evaluate the distribution and expression of actin. NTBo 1935 produced a higher actin degradation and an increased location of this protein at plasma membrane in comparison with A Hall in dependent-time manner. However, at 90 min of treatment, we observed 90% of cytotoxicity and further studies at this time were not evaluated. These results provide new insights about the NTBo mechanism of action and its possible use in the fight against breast cancer.
Fil: Guarniolo, D. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Caballero, P. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Chapana, A.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Sosa, E.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Fernández, R. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Fil: Sosa, M. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Carvelli, Flavia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo - Materia
-
BOTULINUM NEUROTOXIN
BREAST CANCER
ACTIN - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/210158
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The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cellsGuarniolo, DCaballero, P. A.Chapana, A.Sosa, E.Fernández, R. A.Sosa, M. A.Carvelli, Flavia LorenaBOTULINUM NEUROTOXINBREAST CANCERACTINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Botulism is a neuroparalitic disease caused by botulinum neurotoxins (NTBo, serotypes A-G) produced by Clostridium botulinum, whose main reservoir is the soil (Su). Infant botulism is a toxiinfection, caused by ingestion of spores, subsequent colonization and production of toxins in situ. The autochthonous NTBo would correspond to subtype A2, and have higher toxicity than A1 (Botox®), so in the future, they could be used as a therapeutic agent. The NTBos mechanism of action on certain pathologies is still to be clarified. Previous results from our laboratory showed that autochthonous NTBo 1935 from Su, degrades actin of rat brain homogenates, suggesting this protein could be an active target of NTBos. In this work, the action of this NTBo on the actin cytoskeleton in mammary tumor cells was evaluated. The NTBos of Su from strain 1935 and strain A Hall (both serotype A) were purified by saline precipitation. MCF7 cells (breast cancer cells) were cultured in petri dishes or coverslips with 250 LD50 of the NTBos for 25, 45 or 90 min. After incubations, cells were processed for western blot or immunofluorescence in order to evaluate the distribution and expression of actin. NTBo 1935 produced a higher actin degradation and an increased location of this protein at plasma membrane in comparison with A Hall in dependent-time manner. However, at 90 min of treatment, we observed 90% of cytotoxicity and further studies at this time were not evaluated. These results provide new insights about the NTBo mechanism of action and its possible use in the fight against breast cancer.Fil: Guarniolo, D. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Caballero, P. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Chapana, A.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Sosa, E.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Fernández, R. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Sosa, M. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Carvelli, Flavia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaXXXVII Reunión Científica Anual de la Sociedad de Biología de CuyoSan LuisArgentinaSociedad de Biología de CuyoTech Science Press2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/210158The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 14-150327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v44nSuppl.1/39488Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:34:56Zoai:ri.conicet.gov.ar:11336/210158instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:34:57.125CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells |
title |
The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells |
spellingShingle |
The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells Guarniolo, D BOTULINUM NEUROTOXIN BREAST CANCER ACTIN |
title_short |
The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells |
title_full |
The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells |
title_fullStr |
The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells |
title_full_unstemmed |
The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells |
title_sort |
The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells |
dc.creator.none.fl_str_mv |
Guarniolo, D Caballero, P. A. Chapana, A. Sosa, E. Fernández, R. A. Sosa, M. A. Carvelli, Flavia Lorena |
author |
Guarniolo, D |
author_facet |
Guarniolo, D Caballero, P. A. Chapana, A. Sosa, E. Fernández, R. A. Sosa, M. A. Carvelli, Flavia Lorena |
author_role |
author |
author2 |
Caballero, P. A. Chapana, A. Sosa, E. Fernández, R. A. Sosa, M. A. Carvelli, Flavia Lorena |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
BOTULINUM NEUROTOXIN BREAST CANCER ACTIN |
topic |
BOTULINUM NEUROTOXIN BREAST CANCER ACTIN |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Botulism is a neuroparalitic disease caused by botulinum neurotoxins (NTBo, serotypes A-G) produced by Clostridium botulinum, whose main reservoir is the soil (Su). Infant botulism is a toxiinfection, caused by ingestion of spores, subsequent colonization and production of toxins in situ. The autochthonous NTBo would correspond to subtype A2, and have higher toxicity than A1 (Botox®), so in the future, they could be used as a therapeutic agent. The NTBos mechanism of action on certain pathologies is still to be clarified. Previous results from our laboratory showed that autochthonous NTBo 1935 from Su, degrades actin of rat brain homogenates, suggesting this protein could be an active target of NTBos. In this work, the action of this NTBo on the actin cytoskeleton in mammary tumor cells was evaluated. The NTBos of Su from strain 1935 and strain A Hall (both serotype A) were purified by saline precipitation. MCF7 cells (breast cancer cells) were cultured in petri dishes or coverslips with 250 LD50 of the NTBos for 25, 45 or 90 min. After incubations, cells were processed for western blot or immunofluorescence in order to evaluate the distribution and expression of actin. NTBo 1935 produced a higher actin degradation and an increased location of this protein at plasma membrane in comparison with A Hall in dependent-time manner. However, at 90 min of treatment, we observed 90% of cytotoxicity and further studies at this time were not evaluated. These results provide new insights about the NTBo mechanism of action and its possible use in the fight against breast cancer. Fil: Guarniolo, D. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina Fil: Caballero, P. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina Fil: Chapana, A.. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Sosa, E.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina Fil: Fernández, R. A.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina Fil: Sosa, M. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Carvelli, Flavia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Histología y Embriología D/mend Dr.m.burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo San Luis Argentina Sociedad de Biología de Cuyo |
description |
Botulism is a neuroparalitic disease caused by botulinum neurotoxins (NTBo, serotypes A-G) produced by Clostridium botulinum, whose main reservoir is the soil (Su). Infant botulism is a toxiinfection, caused by ingestion of spores, subsequent colonization and production of toxins in situ. The autochthonous NTBo would correspond to subtype A2, and have higher toxicity than A1 (Botox®), so in the future, they could be used as a therapeutic agent. The NTBos mechanism of action on certain pathologies is still to be clarified. Previous results from our laboratory showed that autochthonous NTBo 1935 from Su, degrades actin of rat brain homogenates, suggesting this protein could be an active target of NTBos. In this work, the action of this NTBo on the actin cytoskeleton in mammary tumor cells was evaluated. The NTBos of Su from strain 1935 and strain A Hall (both serotype A) were purified by saline precipitation. MCF7 cells (breast cancer cells) were cultured in petri dishes or coverslips with 250 LD50 of the NTBos for 25, 45 or 90 min. After incubations, cells were processed for western blot or immunofluorescence in order to evaluate the distribution and expression of actin. NTBo 1935 produced a higher actin degradation and an increased location of this protein at plasma membrane in comparison with A Hall in dependent-time manner. However, at 90 min of treatment, we observed 90% of cytotoxicity and further studies at this time were not evaluated. These results provide new insights about the NTBo mechanism of action and its possible use in the fight against breast cancer. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 |
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publishedVersion |
format |
conferenceObject |
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http://hdl.handle.net/11336/210158 The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 14-15 0327-9545 1667-5746 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/210158 |
identifier_str_mv |
The neurotoxin of a autochthonous Clostridium botulinum affects the actin cytoskeleton in breast cancer cells; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 14-15 0327-9545 1667-5746 CONICET Digital CONICET |
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eng |
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Tech Science Press |
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Tech Science Press |
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