Riboflavin acetate induces apoptosis in squamous carcinoma cells after photodynamic therapy

Autores
Juarez, Andrea Virginia; Sosa, Liliana del Valle; de Paul, Ana Lucia; Costa, Ana Paula; Farina, Marcelo; Leal, Rodrigo B.; Torres, Alicia Ines; Pons, Patricia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several research efforts have been focused on finding newer and more efficient photosensitizers for photodynamic therapy (PDT). Although, it was demonstrated that riboflavin is an efficient photosensitizer for PDT, the effect of its ester derivate, riboflavin 2′,3′,4′,5′-tetraacetate (RFTA), which has higher cellular uptake, has not been well defined. To evaluate the cell death generated by applying RFTA as the photosensitizer in PDT in a human cancer cell line of squamous carcinoma (SCC-13), these cells were incubated with riboflavin and its ester derivate, RFTA followed by irradiation with different blue light doses. Cell viability was evaluated using neutral red uptake assay and cell death was evaluated using transmission electron microscopy, TUNEL assay and annexin V-PE/7AAD double staining. The expression of caspase-3, Bax, Bcl-2, ERK 1/2 and p38MAPK was evaluated by Western blotting and generation of intracellular ROS and changes in anion superoxide levels were analyzed using 2′,7′-dichlorofluorescein-diacetate and dihydroethidium dye, respectively. RFTA-PDT generated a decrease in cancer cell viability in a light dose–response. Treated SCC-13 cells exhibited chromatin condensation, formation of apoptotic bodies, increases in TUNEL-positive cells, phosphatidylserine externalization and decreased procaspase-3 and Bcl-2 protein expression and increment of ERK 1/2 phosphorylation. Moreover, trolox abolished the effect of PDT on cell viability linking the increase in intracellular ROS levels with the cell death observed, whereas that the pre-treatment with MEK inhibitor did not induce changes in SCC-13 cell survival. These findings demonstrate the effects of RFTA in triggering apoptosis induced by ROS (\O2−) production after visible light irradiation of squamous carcinoma cells.
Fil: Juarez, Andrea Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Sosa, Liliana del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: de Paul, Ana Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Costa, Ana Paula. Universidade Federal de Santa Catarina; Brasil
Fil: Farina, Marcelo. Universidade Federal de Santa Catarina; Brasil
Fil: Leal, Rodrigo B.. Universidade Federal de Santa Catarina; Brasil
Fil: Torres, Alicia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Pons, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Materia
Photodynamic Therapy
Scc-13 Cells
Riboflavin 2′,3′,4′,5′-Tetraacetate
Apoptosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50591
Acceder
id CONICETDig_ca471626bb466fc00050f7edc9847785
oai_identifier_str oai:ri.conicet.gov.ar:11336/50591
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Riboflavin acetate induces apoptosis in squamous carcinoma cells after photodynamic therapyJuarez, Andrea VirginiaSosa, Liliana del Vallede Paul, Ana LuciaCosta, Ana PaulaFarina, MarceloLeal, Rodrigo B.Torres, Alicia InesPons, PatriciaPhotodynamic TherapyScc-13 CellsRiboflavin 2′,3′,4′,5′-TetraacetateApoptosishttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Several research efforts have been focused on finding newer and more efficient photosensitizers for photodynamic therapy (PDT). Although, it was demonstrated that riboflavin is an efficient photosensitizer for PDT, the effect of its ester derivate, riboflavin 2′,3′,4′,5′-tetraacetate (RFTA), which has higher cellular uptake, has not been well defined. To evaluate the cell death generated by applying RFTA as the photosensitizer in PDT in a human cancer cell line of squamous carcinoma (SCC-13), these cells were incubated with riboflavin and its ester derivate, RFTA followed by irradiation with different blue light doses. Cell viability was evaluated using neutral red uptake assay and cell death was evaluated using transmission electron microscopy, TUNEL assay and annexin V-PE/7AAD double staining. The expression of caspase-3, Bax, Bcl-2, ERK 1/2 and p38MAPK was evaluated by Western blotting and generation of intracellular ROS and changes in anion superoxide levels were analyzed using 2′,7′-dichlorofluorescein-diacetate and dihydroethidium dye, respectively. RFTA-PDT generated a decrease in cancer cell viability in a light dose–response. Treated SCC-13 cells exhibited chromatin condensation, formation of apoptotic bodies, increases in TUNEL-positive cells, phosphatidylserine externalization and decreased procaspase-3 and Bcl-2 protein expression and increment of ERK 1/2 phosphorylation. Moreover, trolox abolished the effect of PDT on cell viability linking the increase in intracellular ROS levels with the cell death observed, whereas that the pre-treatment with MEK inhibitor did not induce changes in SCC-13 cell survival. These findings demonstrate the effects of RFTA in triggering apoptosis induced by ROS (\O2−) production after visible light irradiation of squamous carcinoma cells.Fil: Juarez, Andrea Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Sosa, Liliana del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: de Paul, Ana Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Costa, Ana Paula. Universidade Federal de Santa Catarina; BrasilFil: Farina, Marcelo. Universidade Federal de Santa Catarina; BrasilFil: Leal, Rodrigo B.. Universidade Federal de Santa Catarina; BrasilFil: Torres, Alicia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Pons, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaElsevier Science Sa2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50591Juarez, Andrea Virginia; Sosa, Liliana del Valle; de Paul, Ana Lucia; Costa, Ana Paula; Farina, Marcelo; et al.; Riboflavin acetate induces apoptosis in squamous carcinoma cells after photodynamic therapy; Elsevier Science Sa; Journal of Photochemistry and Photobiology B: Biology; 153; 11-2015; 445-4541011-1344CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1011134415003590info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jphotobiol.2015.10.030info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:53:26Zoai:ri.conicet.gov.ar:11336/50591instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:53:26.915CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Riboflavin acetate induces apoptosis in squamous carcinoma cells after photodynamic therapy
title Riboflavin acetate induces apoptosis in squamous carcinoma cells after photodynamic therapy
spellingShingle Riboflavin acetate induces apoptosis in squamous carcinoma cells after photodynamic therapy
Juarez, Andrea Virginia
Photodynamic Therapy
Scc-13 Cells
Riboflavin 2′,3′,4′,5′-Tetraacetate
Apoptosis
title_short Riboflavin acetate induces apoptosis in squamous carcinoma cells after photodynamic therapy
title_full Riboflavin acetate induces apoptosis in squamous carcinoma cells after photodynamic therapy
title_fullStr Riboflavin acetate induces apoptosis in squamous carcinoma cells after photodynamic therapy
title_full_unstemmed Riboflavin acetate induces apoptosis in squamous carcinoma cells after photodynamic therapy
title_sort Riboflavin acetate induces apoptosis in squamous carcinoma cells after photodynamic therapy
dc.creator.none.fl_str_mv Juarez, Andrea Virginia
Sosa, Liliana del Valle
de Paul, Ana Lucia
Costa, Ana Paula
Farina, Marcelo
Leal, Rodrigo B.
Torres, Alicia Ines
Pons, Patricia
author Juarez, Andrea Virginia
author_facet Juarez, Andrea Virginia
Sosa, Liliana del Valle
de Paul, Ana Lucia
Costa, Ana Paula
Farina, Marcelo
Leal, Rodrigo B.
Torres, Alicia Ines
Pons, Patricia
author_role author
author2 Sosa, Liliana del Valle
de Paul, Ana Lucia
Costa, Ana Paula
Farina, Marcelo
Leal, Rodrigo B.
Torres, Alicia Ines
Pons, Patricia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Photodynamic Therapy
Scc-13 Cells
Riboflavin 2′,3′,4′,5′-Tetraacetate
Apoptosis
topic Photodynamic Therapy
Scc-13 Cells
Riboflavin 2′,3′,4′,5′-Tetraacetate
Apoptosis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Several research efforts have been focused on finding newer and more efficient photosensitizers for photodynamic therapy (PDT). Although, it was demonstrated that riboflavin is an efficient photosensitizer for PDT, the effect of its ester derivate, riboflavin 2′,3′,4′,5′-tetraacetate (RFTA), which has higher cellular uptake, has not been well defined. To evaluate the cell death generated by applying RFTA as the photosensitizer in PDT in a human cancer cell line of squamous carcinoma (SCC-13), these cells were incubated with riboflavin and its ester derivate, RFTA followed by irradiation with different blue light doses. Cell viability was evaluated using neutral red uptake assay and cell death was evaluated using transmission electron microscopy, TUNEL assay and annexin V-PE/7AAD double staining. The expression of caspase-3, Bax, Bcl-2, ERK 1/2 and p38MAPK was evaluated by Western blotting and generation of intracellular ROS and changes in anion superoxide levels were analyzed using 2′,7′-dichlorofluorescein-diacetate and dihydroethidium dye, respectively. RFTA-PDT generated a decrease in cancer cell viability in a light dose–response. Treated SCC-13 cells exhibited chromatin condensation, formation of apoptotic bodies, increases in TUNEL-positive cells, phosphatidylserine externalization and decreased procaspase-3 and Bcl-2 protein expression and increment of ERK 1/2 phosphorylation. Moreover, trolox abolished the effect of PDT on cell viability linking the increase in intracellular ROS levels with the cell death observed, whereas that the pre-treatment with MEK inhibitor did not induce changes in SCC-13 cell survival. These findings demonstrate the effects of RFTA in triggering apoptosis induced by ROS (\O2−) production after visible light irradiation of squamous carcinoma cells.
Fil: Juarez, Andrea Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Sosa, Liliana del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: de Paul, Ana Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Costa, Ana Paula. Universidade Federal de Santa Catarina; Brasil
Fil: Farina, Marcelo. Universidade Federal de Santa Catarina; Brasil
Fil: Leal, Rodrigo B.. Universidade Federal de Santa Catarina; Brasil
Fil: Torres, Alicia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Pons, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
description Several research efforts have been focused on finding newer and more efficient photosensitizers for photodynamic therapy (PDT). Although, it was demonstrated that riboflavin is an efficient photosensitizer for PDT, the effect of its ester derivate, riboflavin 2′,3′,4′,5′-tetraacetate (RFTA), which has higher cellular uptake, has not been well defined. To evaluate the cell death generated by applying RFTA as the photosensitizer in PDT in a human cancer cell line of squamous carcinoma (SCC-13), these cells were incubated with riboflavin and its ester derivate, RFTA followed by irradiation with different blue light doses. Cell viability was evaluated using neutral red uptake assay and cell death was evaluated using transmission electron microscopy, TUNEL assay and annexin V-PE/7AAD double staining. The expression of caspase-3, Bax, Bcl-2, ERK 1/2 and p38MAPK was evaluated by Western blotting and generation of intracellular ROS and changes in anion superoxide levels were analyzed using 2′,7′-dichlorofluorescein-diacetate and dihydroethidium dye, respectively. RFTA-PDT generated a decrease in cancer cell viability in a light dose–response. Treated SCC-13 cells exhibited chromatin condensation, formation of apoptotic bodies, increases in TUNEL-positive cells, phosphatidylserine externalization and decreased procaspase-3 and Bcl-2 protein expression and increment of ERK 1/2 phosphorylation. Moreover, trolox abolished the effect of PDT on cell viability linking the increase in intracellular ROS levels with the cell death observed, whereas that the pre-treatment with MEK inhibitor did not induce changes in SCC-13 cell survival. These findings demonstrate the effects of RFTA in triggering apoptosis induced by ROS (\O2−) production after visible light irradiation of squamous carcinoma cells.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50591
Juarez, Andrea Virginia; Sosa, Liliana del Valle; de Paul, Ana Lucia; Costa, Ana Paula; Farina, Marcelo; et al.; Riboflavin acetate induces apoptosis in squamous carcinoma cells after photodynamic therapy; Elsevier Science Sa; Journal of Photochemistry and Photobiology B: Biology; 153; 11-2015; 445-454
1011-1344
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50591
identifier_str_mv Juarez, Andrea Virginia; Sosa, Liliana del Valle; de Paul, Ana Lucia; Costa, Ana Paula; Farina, Marcelo; et al.; Riboflavin acetate induces apoptosis in squamous carcinoma cells after photodynamic therapy; Elsevier Science Sa; Journal of Photochemistry and Photobiology B: Biology; 153; 11-2015; 445-454
1011-1344
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1011134415003590
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jphotobiol.2015.10.030
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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