Geographical Distribution of Pyrethroid Resistance Allele Frequency in Head Lice (Phthiraptera: Pediculidae) From Argentina

Autores
Toloza, Ariel Ceferino; Ascunce, Marina Sofia; Reed, David; Picollo, Maria Ines
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The human head louse, Pediculus humanus capitis De Geer (Phthiraptera: Pediculidae), is an obligate ectoparasite that causes pediculosis capitis and has parasitized humans since the beginning of humankind. Head louse infestations are widespread throughout the world and have been increasing since the early 1990s partially because of ineffective pediculicides. In Argentina, the overuse of products containing pyrethroids has led to the development of resistant louse populations. Pyrethroid insecticides act on the nervous system affecting voltage-sensitive sodium channels. Three point mutations at the corresponding amino acid sequence positions M815I, T917I, and L920F in the voltage-gated sodium channel gene are responsible for contributing to knockdown resistance (kdr). The management of pyrethroid resistance requires either early detection or the characterization of the mechanisms involved in head louse populations. In the current study, we estimated the distribution of kdr alleles in 154 head lice from six geographical regions of Argentina. Pyrethroid resistance kdr alleles were found in high frequencies ranging from 67 to 100%. Of these, 131 (85.1%) were homozygous resistant, 13 (8.4%) were homozygous susceptible, and 10 (6.5%) were heterozygous. Exact tests for the Hardy-Weinberg equilibrium for each location showed that genotype frequencies differed significantly from expectation in four of the six sites studied. These results show that pyrethroid resistance is well established reaching an overall frequency of 88%, thus close to fixation. With 30 yr of pyrethroid-based pediculicides use in Argentina, kdr resistance has evolved rapidly among these head louse populations.
Fil: Toloza, Ariel Ceferino. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación en Láseres y Aplicaciones; Argentina
Fil: Ascunce, Marina Sofia. University of Florida; Estados Unidos
Fil: Reed, David. University of Florida; Estados Unidos
Fil: Picollo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación en Láseres y Aplicaciones; Argentina
Materia
Head Lice
Knockdown Resistance
Sodium Channel
Pediculus Humanus Capitis
Insecticide Resistance
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29831
Acceder
id CONICETDig_c999b2e5698b55ebbe3ef89ae14d3433
oai_identifier_str oai:ri.conicet.gov.ar:11336/29831
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Geographical Distribution of Pyrethroid Resistance Allele Frequency in Head Lice (Phthiraptera: Pediculidae) From ArgentinaToloza, Ariel CeferinoAscunce, Marina SofiaReed, DavidPicollo, Maria InesHead LiceKnockdown ResistanceSodium ChannelPediculus Humanus CapitisInsecticide Resistancehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The human head louse, Pediculus humanus capitis De Geer (Phthiraptera: Pediculidae), is an obligate ectoparasite that causes pediculosis capitis and has parasitized humans since the beginning of humankind. Head louse infestations are widespread throughout the world and have been increasing since the early 1990s partially because of ineffective pediculicides. In Argentina, the overuse of products containing pyrethroids has led to the development of resistant louse populations. Pyrethroid insecticides act on the nervous system affecting voltage-sensitive sodium channels. Three point mutations at the corresponding amino acid sequence positions M815I, T917I, and L920F in the voltage-gated sodium channel gene are responsible for contributing to knockdown resistance (kdr). The management of pyrethroid resistance requires either early detection or the characterization of the mechanisms involved in head louse populations. In the current study, we estimated the distribution of kdr alleles in 154 head lice from six geographical regions of Argentina. Pyrethroid resistance kdr alleles were found in high frequencies ranging from 67 to 100%. Of these, 131 (85.1%) were homozygous resistant, 13 (8.4%) were homozygous susceptible, and 10 (6.5%) were heterozygous. Exact tests for the Hardy-Weinberg equilibrium for each location showed that genotype frequencies differed significantly from expectation in four of the six sites studied. These results show that pyrethroid resistance is well established reaching an overall frequency of 88%, thus close to fixation. With 30 yr of pyrethroid-based pediculicides use in Argentina, kdr resistance has evolved rapidly among these head louse populations.Fil: Toloza, Ariel Ceferino. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación en Láseres y Aplicaciones; ArgentinaFil: Ascunce, Marina Sofia. University of Florida; Estados UnidosFil: Reed, David. University of Florida; Estados UnidosFil: Picollo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación en Láseres y Aplicaciones; ArgentinaEntomological Society of America2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29831Toloza, Ariel Ceferino; Ascunce, Marina Sofia; Reed, David; Picollo, Maria Ines; Geographical Distribution of Pyrethroid Resistance Allele Frequency in Head Lice (Phthiraptera: Pediculidae) From Argentina; Entomological Society of America; Journal of Medical Entomology; 51; 1; 9-2013; 139-1440022-25851938-2928CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1603/ME13138info:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/doi/abs/10.1603%2FME13138info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:06:32Zoai:ri.conicet.gov.ar:11336/29831instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:06:32.48CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Geographical Distribution of Pyrethroid Resistance Allele Frequency in Head Lice (Phthiraptera: Pediculidae) From Argentina
title Geographical Distribution of Pyrethroid Resistance Allele Frequency in Head Lice (Phthiraptera: Pediculidae) From Argentina
spellingShingle Geographical Distribution of Pyrethroid Resistance Allele Frequency in Head Lice (Phthiraptera: Pediculidae) From Argentina
Toloza, Ariel Ceferino
Head Lice
Knockdown Resistance
Sodium Channel
Pediculus Humanus Capitis
Insecticide Resistance
title_short Geographical Distribution of Pyrethroid Resistance Allele Frequency in Head Lice (Phthiraptera: Pediculidae) From Argentina
title_full Geographical Distribution of Pyrethroid Resistance Allele Frequency in Head Lice (Phthiraptera: Pediculidae) From Argentina
title_fullStr Geographical Distribution of Pyrethroid Resistance Allele Frequency in Head Lice (Phthiraptera: Pediculidae) From Argentina
title_full_unstemmed Geographical Distribution of Pyrethroid Resistance Allele Frequency in Head Lice (Phthiraptera: Pediculidae) From Argentina
title_sort Geographical Distribution of Pyrethroid Resistance Allele Frequency in Head Lice (Phthiraptera: Pediculidae) From Argentina
dc.creator.none.fl_str_mv Toloza, Ariel Ceferino
Ascunce, Marina Sofia
Reed, David
Picollo, Maria Ines
author Toloza, Ariel Ceferino
author_facet Toloza, Ariel Ceferino
Ascunce, Marina Sofia
Reed, David
Picollo, Maria Ines
author_role author
author2 Ascunce, Marina Sofia
Reed, David
Picollo, Maria Ines
author2_role author
author
author
dc.subject.none.fl_str_mv Head Lice
Knockdown Resistance
Sodium Channel
Pediculus Humanus Capitis
Insecticide Resistance
topic Head Lice
Knockdown Resistance
Sodium Channel
Pediculus Humanus Capitis
Insecticide Resistance
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The human head louse, Pediculus humanus capitis De Geer (Phthiraptera: Pediculidae), is an obligate ectoparasite that causes pediculosis capitis and has parasitized humans since the beginning of humankind. Head louse infestations are widespread throughout the world and have been increasing since the early 1990s partially because of ineffective pediculicides. In Argentina, the overuse of products containing pyrethroids has led to the development of resistant louse populations. Pyrethroid insecticides act on the nervous system affecting voltage-sensitive sodium channels. Three point mutations at the corresponding amino acid sequence positions M815I, T917I, and L920F in the voltage-gated sodium channel gene are responsible for contributing to knockdown resistance (kdr). The management of pyrethroid resistance requires either early detection or the characterization of the mechanisms involved in head louse populations. In the current study, we estimated the distribution of kdr alleles in 154 head lice from six geographical regions of Argentina. Pyrethroid resistance kdr alleles were found in high frequencies ranging from 67 to 100%. Of these, 131 (85.1%) were homozygous resistant, 13 (8.4%) were homozygous susceptible, and 10 (6.5%) were heterozygous. Exact tests for the Hardy-Weinberg equilibrium for each location showed that genotype frequencies differed significantly from expectation in four of the six sites studied. These results show that pyrethroid resistance is well established reaching an overall frequency of 88%, thus close to fixation. With 30 yr of pyrethroid-based pediculicides use in Argentina, kdr resistance has evolved rapidly among these head louse populations.
Fil: Toloza, Ariel Ceferino. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación en Láseres y Aplicaciones; Argentina
Fil: Ascunce, Marina Sofia. University of Florida; Estados Unidos
Fil: Reed, David. University of Florida; Estados Unidos
Fil: Picollo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Investigación y Desarrollo Estratégico para la Defensa. Ministerio de Defensa. Unidad de Investigación y Desarrollo Estratégico para la Defensa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación en Láseres y Aplicaciones; Argentina
description The human head louse, Pediculus humanus capitis De Geer (Phthiraptera: Pediculidae), is an obligate ectoparasite that causes pediculosis capitis and has parasitized humans since the beginning of humankind. Head louse infestations are widespread throughout the world and have been increasing since the early 1990s partially because of ineffective pediculicides. In Argentina, the overuse of products containing pyrethroids has led to the development of resistant louse populations. Pyrethroid insecticides act on the nervous system affecting voltage-sensitive sodium channels. Three point mutations at the corresponding amino acid sequence positions M815I, T917I, and L920F in the voltage-gated sodium channel gene are responsible for contributing to knockdown resistance (kdr). The management of pyrethroid resistance requires either early detection or the characterization of the mechanisms involved in head louse populations. In the current study, we estimated the distribution of kdr alleles in 154 head lice from six geographical regions of Argentina. Pyrethroid resistance kdr alleles were found in high frequencies ranging from 67 to 100%. Of these, 131 (85.1%) were homozygous resistant, 13 (8.4%) were homozygous susceptible, and 10 (6.5%) were heterozygous. Exact tests for the Hardy-Weinberg equilibrium for each location showed that genotype frequencies differed significantly from expectation in four of the six sites studied. These results show that pyrethroid resistance is well established reaching an overall frequency of 88%, thus close to fixation. With 30 yr of pyrethroid-based pediculicides use in Argentina, kdr resistance has evolved rapidly among these head louse populations.
publishDate 2013
dc.date.none.fl_str_mv 2013-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29831
Toloza, Ariel Ceferino; Ascunce, Marina Sofia; Reed, David; Picollo, Maria Ines; Geographical Distribution of Pyrethroid Resistance Allele Frequency in Head Lice (Phthiraptera: Pediculidae) From Argentina; Entomological Society of America; Journal of Medical Entomology; 51; 1; 9-2013; 139-144
0022-2585
1938-2928
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29831
identifier_str_mv Toloza, Ariel Ceferino; Ascunce, Marina Sofia; Reed, David; Picollo, Maria Ines; Geographical Distribution of Pyrethroid Resistance Allele Frequency in Head Lice (Phthiraptera: Pediculidae) From Argentina; Entomological Society of America; Journal of Medical Entomology; 51; 1; 9-2013; 139-144
0022-2585
1938-2928
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1603/ME13138
info:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/doi/abs/10.1603%2FME13138
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Entomological Society of America
publisher.none.fl_str_mv Entomological Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980273362305024
score 12.993085

Publicaciones similares