Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies

Autores
Mendisco, Fanny; Keyser, Christine; Hollard, Clémence; Seldes, Verónica; Nielsen, Axel Emil; Crubézy, Eric; Ludes, Bertrand
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Important developments in the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique have generated new perspectives regarding SNP genotyping, which are particularly promising for ancient population-based studies. The main aim of the present study was to investigate the application of a MALDI-TOF MS-based SNP genotyping technique, called iPLEX® Gold, to analyze Amerindian ancient DNA samples. The first objective was to test the sensitivity of the method, which is recommended for DNA quantities between 10 and 5ng, for ancient biological samples containing DNA molecules that were degraded and present in minute quantities. The second objective was to detail the advantages of this technique for studies on ancient populations. Two multiplexes were designed, allowing the major Amerindian mitochondrial and Y haplogroups to be determined simultaneously. This analysis has never been described before. Results demonstrated the reliability and accuracy of the method; data were obtained for both mitochondrial and nuclear DNA using picogram (pg) quantities of nucleic acid. This technique has the advantages of both MS and minisequencing techniques; thus, it should be included in the protocols for future ancient DNA studies.
Fil: Mendisco, Fanny. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; Francia
Fil: Keyser, Christine. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; Francia
Fil: Hollard, Clémence. Université de Strasbourg; Francia
Fil: Seldes, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Secretaría de Cultura de la Nación. Dirección Nacional de Cultura y Museos. Instituto Nacional de Antropología y Pensamiento Latinoamericano; Argentina
Fil: Nielsen, Axel Emil. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Secretaría de Cultura de la Nación. Dirección Nacional de Cultura y Museos. Instituto Nacional de Antropología y Pensamiento Latinoamericano; Argentina
Fil: Crubézy, Eric. Centre National de la Recherche Scientifique; Francia
Fil: Ludes, Bertrand. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; Francia
Materia
ANCIENT DNA
MALDI-TOF MS
MITOCHONDRIAL HAPLOGROUP
SNP GENOTYPING
Y HAPLOGROUP
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189082
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/189082
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studiesMendisco, FannyKeyser, ChristineHollard, ClémenceSeldes, VerónicaNielsen, Axel EmilCrubézy, EricLudes, BertrandANCIENT DNAMALDI-TOF MSMITOCHONDRIAL HAPLOGROUPSNP GENOTYPINGY HAPLOGROUPhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Important developments in the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique have generated new perspectives regarding SNP genotyping, which are particularly promising for ancient population-based studies. The main aim of the present study was to investigate the application of a MALDI-TOF MS-based SNP genotyping technique, called iPLEX® Gold, to analyze Amerindian ancient DNA samples. The first objective was to test the sensitivity of the method, which is recommended for DNA quantities between 10 and 5ng, for ancient biological samples containing DNA molecules that were degraded and present in minute quantities. The second objective was to detail the advantages of this technique for studies on ancient populations. Two multiplexes were designed, allowing the major Amerindian mitochondrial and Y haplogroups to be determined simultaneously. This analysis has never been described before. Results demonstrated the reliability and accuracy of the method; data were obtained for both mitochondrial and nuclear DNA using picogram (pg) quantities of nucleic acid. This technique has the advantages of both MS and minisequencing techniques; thus, it should be included in the protocols for future ancient DNA studies.Fil: Mendisco, Fanny. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; FranciaFil: Keyser, Christine. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; FranciaFil: Hollard, Clémence. Université de Strasbourg; FranciaFil: Seldes, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Secretaría de Cultura de la Nación. Dirección Nacional de Cultura y Museos. Instituto Nacional de Antropología y Pensamiento Latinoamericano; ArgentinaFil: Nielsen, Axel Emil. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Secretaría de Cultura de la Nación. Dirección Nacional de Cultura y Museos. Instituto Nacional de Antropología y Pensamiento Latinoamericano; ArgentinaFil: Crubézy, Eric. Centre National de la Recherche Scientifique; FranciaFil: Ludes, Bertrand. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; FranciaWiley VCH Verlag2011-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189082Mendisco, Fanny; Keyser, Christine; Hollard, Clémence; Seldes, Verónica; Nielsen, Axel Emil; et al.; Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies; Wiley VCH Verlag; Electrophoresis; 32; 3-4; 1-2011; 386-3930173-0835CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elps.201000483info:eu-repo/semantics/altIdentifier/doi/ 10.1002/elps.201000483info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:37:09Zoai:ri.conicet.gov.ar:11336/189082instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:37:09.492CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies
title Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies
spellingShingle Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies
Mendisco, Fanny
ANCIENT DNA
MALDI-TOF MS
MITOCHONDRIAL HAPLOGROUP
SNP GENOTYPING
Y HAPLOGROUP
title_short Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies
title_full Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies
title_fullStr Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies
title_full_unstemmed Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies
title_sort Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies
dc.creator.none.fl_str_mv Mendisco, Fanny
Keyser, Christine
Hollard, Clémence
Seldes, Verónica
Nielsen, Axel Emil
Crubézy, Eric
Ludes, Bertrand
author Mendisco, Fanny
author_facet Mendisco, Fanny
Keyser, Christine
Hollard, Clémence
Seldes, Verónica
Nielsen, Axel Emil
Crubézy, Eric
Ludes, Bertrand
author_role author
author2 Keyser, Christine
Hollard, Clémence
Seldes, Verónica
Nielsen, Axel Emil
Crubézy, Eric
Ludes, Bertrand
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANCIENT DNA
MALDI-TOF MS
MITOCHONDRIAL HAPLOGROUP
SNP GENOTYPING
Y HAPLOGROUP
topic ANCIENT DNA
MALDI-TOF MS
MITOCHONDRIAL HAPLOGROUP
SNP GENOTYPING
Y HAPLOGROUP
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Important developments in the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique have generated new perspectives regarding SNP genotyping, which are particularly promising for ancient population-based studies. The main aim of the present study was to investigate the application of a MALDI-TOF MS-based SNP genotyping technique, called iPLEX® Gold, to analyze Amerindian ancient DNA samples. The first objective was to test the sensitivity of the method, which is recommended for DNA quantities between 10 and 5ng, for ancient biological samples containing DNA molecules that were degraded and present in minute quantities. The second objective was to detail the advantages of this technique for studies on ancient populations. Two multiplexes were designed, allowing the major Amerindian mitochondrial and Y haplogroups to be determined simultaneously. This analysis has never been described before. Results demonstrated the reliability and accuracy of the method; data were obtained for both mitochondrial and nuclear DNA using picogram (pg) quantities of nucleic acid. This technique has the advantages of both MS and minisequencing techniques; thus, it should be included in the protocols for future ancient DNA studies.
Fil: Mendisco, Fanny. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; Francia
Fil: Keyser, Christine. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; Francia
Fil: Hollard, Clémence. Université de Strasbourg; Francia
Fil: Seldes, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Secretaría de Cultura de la Nación. Dirección Nacional de Cultura y Museos. Instituto Nacional de Antropología y Pensamiento Latinoamericano; Argentina
Fil: Nielsen, Axel Emil. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Secretaría de Cultura de la Nación. Dirección Nacional de Cultura y Museos. Instituto Nacional de Antropología y Pensamiento Latinoamericano; Argentina
Fil: Crubézy, Eric. Centre National de la Recherche Scientifique; Francia
Fil: Ludes, Bertrand. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; Francia
description Important developments in the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique have generated new perspectives regarding SNP genotyping, which are particularly promising for ancient population-based studies. The main aim of the present study was to investigate the application of a MALDI-TOF MS-based SNP genotyping technique, called iPLEX® Gold, to analyze Amerindian ancient DNA samples. The first objective was to test the sensitivity of the method, which is recommended for DNA quantities between 10 and 5ng, for ancient biological samples containing DNA molecules that were degraded and present in minute quantities. The second objective was to detail the advantages of this technique for studies on ancient populations. Two multiplexes were designed, allowing the major Amerindian mitochondrial and Y haplogroups to be determined simultaneously. This analysis has never been described before. Results demonstrated the reliability and accuracy of the method; data were obtained for both mitochondrial and nuclear DNA using picogram (pg) quantities of nucleic acid. This technique has the advantages of both MS and minisequencing techniques; thus, it should be included in the protocols for future ancient DNA studies.
publishDate 2011
dc.date.none.fl_str_mv 2011-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189082
Mendisco, Fanny; Keyser, Christine; Hollard, Clémence; Seldes, Verónica; Nielsen, Axel Emil; et al.; Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies; Wiley VCH Verlag; Electrophoresis; 32; 3-4; 1-2011; 386-393
0173-0835
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189082
identifier_str_mv Mendisco, Fanny; Keyser, Christine; Hollard, Clémence; Seldes, Verónica; Nielsen, Axel Emil; et al.; Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies; Wiley VCH Verlag; Electrophoresis; 32; 3-4; 1-2011; 386-393
0173-0835
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elps.201000483
info:eu-repo/semantics/altIdentifier/doi/ 10.1002/elps.201000483
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782018073919488
score 12.982451

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