Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies
- Autores
- Mendisco, Fanny; Keyser, Christine; Hollard, Clémence; Seldes, Verónica; Nielsen, Axel Emil; Crubézy, Eric; Ludes, Bertrand
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Important developments in the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique have generated new perspectives regarding SNP genotyping, which are particularly promising for ancient population-based studies. The main aim of the present study was to investigate the application of a MALDI-TOF MS-based SNP genotyping technique, called iPLEX® Gold, to analyze Amerindian ancient DNA samples. The first objective was to test the sensitivity of the method, which is recommended for DNA quantities between 10 and 5ng, for ancient biological samples containing DNA molecules that were degraded and present in minute quantities. The second objective was to detail the advantages of this technique for studies on ancient populations. Two multiplexes were designed, allowing the major Amerindian mitochondrial and Y haplogroups to be determined simultaneously. This analysis has never been described before. Results demonstrated the reliability and accuracy of the method; data were obtained for both mitochondrial and nuclear DNA using picogram (pg) quantities of nucleic acid. This technique has the advantages of both MS and minisequencing techniques; thus, it should be included in the protocols for future ancient DNA studies.
Fil: Mendisco, Fanny. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; Francia
Fil: Keyser, Christine. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; Francia
Fil: Hollard, Clémence. Université de Strasbourg; Francia
Fil: Seldes, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Secretaría de Cultura de la Nación. Dirección Nacional de Cultura y Museos. Instituto Nacional de Antropología y Pensamiento Latinoamericano; Argentina
Fil: Nielsen, Axel Emil. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Secretaría de Cultura de la Nación. Dirección Nacional de Cultura y Museos. Instituto Nacional de Antropología y Pensamiento Latinoamericano; Argentina
Fil: Crubézy, Eric. Centre National de la Recherche Scientifique; Francia
Fil: Ludes, Bertrand. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; Francia - Materia
-
ANCIENT DNA
MALDI-TOF MS
MITOCHONDRIAL HAPLOGROUP
SNP GENOTYPING
Y HAPLOGROUP - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/189082
Ver los metadatos del registro completo
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Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studiesMendisco, FannyKeyser, ChristineHollard, ClémenceSeldes, VerónicaNielsen, Axel EmilCrubézy, EricLudes, BertrandANCIENT DNAMALDI-TOF MSMITOCHONDRIAL HAPLOGROUPSNP GENOTYPINGY HAPLOGROUPhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Important developments in the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique have generated new perspectives regarding SNP genotyping, which are particularly promising for ancient population-based studies. The main aim of the present study was to investigate the application of a MALDI-TOF MS-based SNP genotyping technique, called iPLEX® Gold, to analyze Amerindian ancient DNA samples. The first objective was to test the sensitivity of the method, which is recommended for DNA quantities between 10 and 5ng, for ancient biological samples containing DNA molecules that were degraded and present in minute quantities. The second objective was to detail the advantages of this technique for studies on ancient populations. Two multiplexes were designed, allowing the major Amerindian mitochondrial and Y haplogroups to be determined simultaneously. This analysis has never been described before. Results demonstrated the reliability and accuracy of the method; data were obtained for both mitochondrial and nuclear DNA using picogram (pg) quantities of nucleic acid. This technique has the advantages of both MS and minisequencing techniques; thus, it should be included in the protocols for future ancient DNA studies.Fil: Mendisco, Fanny. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; FranciaFil: Keyser, Christine. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; FranciaFil: Hollard, Clémence. Université de Strasbourg; FranciaFil: Seldes, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Secretaría de Cultura de la Nación. Dirección Nacional de Cultura y Museos. Instituto Nacional de Antropología y Pensamiento Latinoamericano; ArgentinaFil: Nielsen, Axel Emil. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Secretaría de Cultura de la Nación. Dirección Nacional de Cultura y Museos. Instituto Nacional de Antropología y Pensamiento Latinoamericano; ArgentinaFil: Crubézy, Eric. Centre National de la Recherche Scientifique; FranciaFil: Ludes, Bertrand. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; FranciaWiley VCH Verlag2011-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189082Mendisco, Fanny; Keyser, Christine; Hollard, Clémence; Seldes, Verónica; Nielsen, Axel Emil; et al.; Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies; Wiley VCH Verlag; Electrophoresis; 32; 3-4; 1-2011; 386-3930173-0835CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elps.201000483info:eu-repo/semantics/altIdentifier/doi/ 10.1002/elps.201000483info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:07:14Zoai:ri.conicet.gov.ar:11336/189082instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:07:14.592CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies |
title |
Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies |
spellingShingle |
Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies Mendisco, Fanny ANCIENT DNA MALDI-TOF MS MITOCHONDRIAL HAPLOGROUP SNP GENOTYPING Y HAPLOGROUP |
title_short |
Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies |
title_full |
Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies |
title_fullStr |
Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies |
title_full_unstemmed |
Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies |
title_sort |
Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies |
dc.creator.none.fl_str_mv |
Mendisco, Fanny Keyser, Christine Hollard, Clémence Seldes, Verónica Nielsen, Axel Emil Crubézy, Eric Ludes, Bertrand |
author |
Mendisco, Fanny |
author_facet |
Mendisco, Fanny Keyser, Christine Hollard, Clémence Seldes, Verónica Nielsen, Axel Emil Crubézy, Eric Ludes, Bertrand |
author_role |
author |
author2 |
Keyser, Christine Hollard, Clémence Seldes, Verónica Nielsen, Axel Emil Crubézy, Eric Ludes, Bertrand |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
ANCIENT DNA MALDI-TOF MS MITOCHONDRIAL HAPLOGROUP SNP GENOTYPING Y HAPLOGROUP |
topic |
ANCIENT DNA MALDI-TOF MS MITOCHONDRIAL HAPLOGROUP SNP GENOTYPING Y HAPLOGROUP |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Important developments in the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique have generated new perspectives regarding SNP genotyping, which are particularly promising for ancient population-based studies. The main aim of the present study was to investigate the application of a MALDI-TOF MS-based SNP genotyping technique, called iPLEX® Gold, to analyze Amerindian ancient DNA samples. The first objective was to test the sensitivity of the method, which is recommended for DNA quantities between 10 and 5ng, for ancient biological samples containing DNA molecules that were degraded and present in minute quantities. The second objective was to detail the advantages of this technique for studies on ancient populations. Two multiplexes were designed, allowing the major Amerindian mitochondrial and Y haplogroups to be determined simultaneously. This analysis has never been described before. Results demonstrated the reliability and accuracy of the method; data were obtained for both mitochondrial and nuclear DNA using picogram (pg) quantities of nucleic acid. This technique has the advantages of both MS and minisequencing techniques; thus, it should be included in the protocols for future ancient DNA studies. Fil: Mendisco, Fanny. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; Francia Fil: Keyser, Christine. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; Francia Fil: Hollard, Clémence. Université de Strasbourg; Francia Fil: Seldes, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Secretaría de Cultura de la Nación. Dirección Nacional de Cultura y Museos. Instituto Nacional de Antropología y Pensamiento Latinoamericano; Argentina Fil: Nielsen, Axel Emil. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Secretaría de Cultura de la Nación. Dirección Nacional de Cultura y Museos. Instituto Nacional de Antropología y Pensamiento Latinoamericano; Argentina Fil: Crubézy, Eric. Centre National de la Recherche Scientifique; Francia Fil: Ludes, Bertrand. Centre National de la Recherche Scientifique; Francia. Université de Strasbourg; Francia |
description |
Important developments in the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique have generated new perspectives regarding SNP genotyping, which are particularly promising for ancient population-based studies. The main aim of the present study was to investigate the application of a MALDI-TOF MS-based SNP genotyping technique, called iPLEX® Gold, to analyze Amerindian ancient DNA samples. The first objective was to test the sensitivity of the method, which is recommended for DNA quantities between 10 and 5ng, for ancient biological samples containing DNA molecules that were degraded and present in minute quantities. The second objective was to detail the advantages of this technique for studies on ancient populations. Two multiplexes were designed, allowing the major Amerindian mitochondrial and Y haplogroups to be determined simultaneously. This analysis has never been described before. Results demonstrated the reliability and accuracy of the method; data were obtained for both mitochondrial and nuclear DNA using picogram (pg) quantities of nucleic acid. This technique has the advantages of both MS and minisequencing techniques; thus, it should be included in the protocols for future ancient DNA studies. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/189082 Mendisco, Fanny; Keyser, Christine; Hollard, Clémence; Seldes, Verónica; Nielsen, Axel Emil; et al.; Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies; Wiley VCH Verlag; Electrophoresis; 32; 3-4; 1-2011; 386-393 0173-0835 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/189082 |
identifier_str_mv |
Mendisco, Fanny; Keyser, Christine; Hollard, Clémence; Seldes, Verónica; Nielsen, Axel Emil; et al.; Application of the iPLEXTM Gold SNP genotyping method for the analysis of Amerindian ancient DNA samples: Benefits for ancient population studies; Wiley VCH Verlag; Electrophoresis; 32; 3-4; 1-2011; 386-393 0173-0835 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elps.201000483 info:eu-repo/semantics/altIdentifier/doi/ 10.1002/elps.201000483 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley VCH Verlag |
publisher.none.fl_str_mv |
Wiley VCH Verlag |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613930035445760 |
score |
13.070432 |