Label-free proteomic analysis of the hexachlorocyclohexane-degrading actinobacteria Streptomyces sp. M7

Autores
Dávila Costa, José Sebastián; Sineli, Pedro Eugenio; Cuozzo, Sergio Antonio
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The increment of the global population and subsequently the increasing demand of food has promoted the use of pesticides such as hexachlorocyclohexane (HCH). HCH is a chlorinated pesticide used to protect crops from vector borne diseases. Large-scale production and the extensive use of this compound led to deterioration of environmental quality owing to its persistence in the environment. Over the last years, bacteria-mediated degradation of toxic organic compounds has become in an effective biotechnological process. Streptomyces sp. M7 was isolated from polluted sediments in the province of Tucumán, Argentina. Several physiological studies demonstrated the ability of M7 to remove HCH and highlighted the potential of this strain to be used in bioremediation processes. Degradation pathways for HCH and organochlorine compounds in general, are unknown in Actinobacteria. So far, a mechanism for the degradation of HCH was only proposed in Sphingobium japonicum. MS-based proteomics have become a powerful tool to elucidate and understand the mechanisms that underlie physiological processes. In the present work we used a MS-based, label-free, and quantitative proteomic approach as a starting point for understanding the degradation pathway for HCH in our strain. M7 proteome showed 293 proteins that were significantly up-regulated in the presence of HCH. Key enzymes involved in the dechlorination of HCH (LinA; LinB and LinC) were identified. In addition, 8 proteins assigned to the xenobiotic degradation category could be involved in the so called downstream degradation pathway of HCH. Proteomic results support the physiological capacity of Streptomyces sp. M7 to degrade hexachlorocyclohexane. In turn, the obtained results will allow to postulate a degradative pathway for this bacterium.
Fil: Dávila Costa, José Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Sineli, Pedro Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
LXII Reunión Anual de la Sociedad Argentina De Investigación Clínica; LXV Reunión Anual de la Sociedad Argentina De Inmunología; XLIX Reunión Anual de la Sociedad Argentina De Farmacología Experimental; LIII Reunión Anual de la Sociedad Argentina De Investigación Bioquímica Y Biología Molecular; Reunión Anual de la Sociedad Argentina De Fisiología y XXIX Reunión Anual de la Sociedad Argentina De Protozoología
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina De Investigación Bioquímica Y Biología Molecular
Sociedad Argentina De Investigación Clínica
Sociedad Argentina De Fisiología
Sociedad Argentina De Protozoología
Sociedad Argentina De Farmacología Experimental
Sociedad Argentina de Inmunologìa
Sociedad Argentina de Andrología
Sociedad Argentina de Biofísica
Sociedad Argentina de Biología
Sociedad Argentina de Hematología
Materia
HEXACHLOROCYCLOHEXANE
DEGRADING
ACTINOBACTERIA
STREPTOMYCES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195120
Acceder
id CONICETDig_c8f020d8fad7f4c68d4102da5c3c5642
oai_identifier_str oai:ri.conicet.gov.ar:11336/195120
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Label-free proteomic analysis of the hexachlorocyclohexane-degrading actinobacteria Streptomyces sp. M7Dávila Costa, José SebastiánSineli, Pedro EugenioCuozzo, Sergio AntonioHEXACHLOROCYCLOHEXANEDEGRADINGACTINOBACTERIASTREPTOMYCEShttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The increment of the global population and subsequently the increasing demand of food has promoted the use of pesticides such as hexachlorocyclohexane (HCH). HCH is a chlorinated pesticide used to protect crops from vector borne diseases. Large-scale production and the extensive use of this compound led to deterioration of environmental quality owing to its persistence in the environment. Over the last years, bacteria-mediated degradation of toxic organic compounds has become in an effective biotechnological process. Streptomyces sp. M7 was isolated from polluted sediments in the province of Tucumán, Argentina. Several physiological studies demonstrated the ability of M7 to remove HCH and highlighted the potential of this strain to be used in bioremediation processes. Degradation pathways for HCH and organochlorine compounds in general, are unknown in Actinobacteria. So far, a mechanism for the degradation of HCH was only proposed in Sphingobium japonicum. MS-based proteomics have become a powerful tool to elucidate and understand the mechanisms that underlie physiological processes. In the present work we used a MS-based, label-free, and quantitative proteomic approach as a starting point for understanding the degradation pathway for HCH in our strain. M7 proteome showed 293 proteins that were significantly up-regulated in the presence of HCH. Key enzymes involved in the dechlorination of HCH (LinA; LinB and LinC) were identified. In addition, 8 proteins assigned to the xenobiotic degradation category could be involved in the so called downstream degradation pathway of HCH. Proteomic results support the physiological capacity of Streptomyces sp. M7 to degrade hexachlorocyclohexane. In turn, the obtained results will allow to postulate a degradative pathway for this bacterium.Fil: Dávila Costa, José Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Sineli, Pedro Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaLXII Reunión Anual de la Sociedad Argentina De Investigación Clínica; LXV Reunión Anual de la Sociedad Argentina De Inmunología; XLIX Reunión Anual de la Sociedad Argentina De Farmacología Experimental; LIII Reunión Anual de la Sociedad Argentina De Investigación Bioquímica Y Biología Molecular; Reunión Anual de la Sociedad Argentina De Fisiología y XXIX Reunión Anual de la Sociedad Argentina De ProtozoologíaCiudad Autónoma de Buenos AiresArgentinaSociedad Argentina De Investigación Bioquímica Y Biología MolecularSociedad Argentina De Investigación ClínicaSociedad Argentina De FisiologíaSociedad Argentina De ProtozoologíaSociedad Argentina De Farmacología ExperimentalSociedad Argentina de InmunologìaSociedad Argentina de AndrologíaSociedad Argentina de BiofísicaSociedad Argentina de BiologíaSociedad Argentina de HematologíaFundación Revista Medicina2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195120Label-free proteomic analysis of the hexachlorocyclohexane-degrading actinobacteria Streptomyces sp. M7; LXII Reunión Anual de la Sociedad Argentina De Investigación Clínica; LXV Reunión Anual de la Sociedad Argentina De Inmunología; XLIX Reunión Anual de la Sociedad Argentina De Farmacología Experimental; LIII Reunión Anual de la Sociedad Argentina De Investigación Bioquímica Y Biología Molecular; Reunión Anual de la Sociedad Argentina De Fisiología y XXIX Reunión Anual de la Sociedad Argentina De Protozoología; Ciudad Autónoma de Buenos Aires; Argentina; 2017; 382-382CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol77-17/Vol.77SuplementoI-2017.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:21:12Zoai:ri.conicet.gov.ar:11336/195120instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:21:12.514CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Label-free proteomic analysis of the hexachlorocyclohexane-degrading actinobacteria Streptomyces sp. M7
title Label-free proteomic analysis of the hexachlorocyclohexane-degrading actinobacteria Streptomyces sp. M7
spellingShingle Label-free proteomic analysis of the hexachlorocyclohexane-degrading actinobacteria Streptomyces sp. M7
Dávila Costa, José Sebastián
HEXACHLOROCYCLOHEXANE
DEGRADING
ACTINOBACTERIA
STREPTOMYCES
title_short Label-free proteomic analysis of the hexachlorocyclohexane-degrading actinobacteria Streptomyces sp. M7
title_full Label-free proteomic analysis of the hexachlorocyclohexane-degrading actinobacteria Streptomyces sp. M7
title_fullStr Label-free proteomic analysis of the hexachlorocyclohexane-degrading actinobacteria Streptomyces sp. M7
title_full_unstemmed Label-free proteomic analysis of the hexachlorocyclohexane-degrading actinobacteria Streptomyces sp. M7
title_sort Label-free proteomic analysis of the hexachlorocyclohexane-degrading actinobacteria Streptomyces sp. M7
dc.creator.none.fl_str_mv Dávila Costa, José Sebastián
Sineli, Pedro Eugenio
Cuozzo, Sergio Antonio
author Dávila Costa, José Sebastián
author_facet Dávila Costa, José Sebastián
Sineli, Pedro Eugenio
Cuozzo, Sergio Antonio
author_role author
author2 Sineli, Pedro Eugenio
Cuozzo, Sergio Antonio
author2_role author
author
dc.subject.none.fl_str_mv HEXACHLOROCYCLOHEXANE
DEGRADING
ACTINOBACTERIA
STREPTOMYCES
topic HEXACHLOROCYCLOHEXANE
DEGRADING
ACTINOBACTERIA
STREPTOMYCES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The increment of the global population and subsequently the increasing demand of food has promoted the use of pesticides such as hexachlorocyclohexane (HCH). HCH is a chlorinated pesticide used to protect crops from vector borne diseases. Large-scale production and the extensive use of this compound led to deterioration of environmental quality owing to its persistence in the environment. Over the last years, bacteria-mediated degradation of toxic organic compounds has become in an effective biotechnological process. Streptomyces sp. M7 was isolated from polluted sediments in the province of Tucumán, Argentina. Several physiological studies demonstrated the ability of M7 to remove HCH and highlighted the potential of this strain to be used in bioremediation processes. Degradation pathways for HCH and organochlorine compounds in general, are unknown in Actinobacteria. So far, a mechanism for the degradation of HCH was only proposed in Sphingobium japonicum. MS-based proteomics have become a powerful tool to elucidate and understand the mechanisms that underlie physiological processes. In the present work we used a MS-based, label-free, and quantitative proteomic approach as a starting point for understanding the degradation pathway for HCH in our strain. M7 proteome showed 293 proteins that were significantly up-regulated in the presence of HCH. Key enzymes involved in the dechlorination of HCH (LinA; LinB and LinC) were identified. In addition, 8 proteins assigned to the xenobiotic degradation category could be involved in the so called downstream degradation pathway of HCH. Proteomic results support the physiological capacity of Streptomyces sp. M7 to degrade hexachlorocyclohexane. In turn, the obtained results will allow to postulate a degradative pathway for this bacterium.
Fil: Dávila Costa, José Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Sineli, Pedro Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
LXII Reunión Anual de la Sociedad Argentina De Investigación Clínica; LXV Reunión Anual de la Sociedad Argentina De Inmunología; XLIX Reunión Anual de la Sociedad Argentina De Farmacología Experimental; LIII Reunión Anual de la Sociedad Argentina De Investigación Bioquímica Y Biología Molecular; Reunión Anual de la Sociedad Argentina De Fisiología y XXIX Reunión Anual de la Sociedad Argentina De Protozoología
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina De Investigación Bioquímica Y Biología Molecular
Sociedad Argentina De Investigación Clínica
Sociedad Argentina De Fisiología
Sociedad Argentina De Protozoología
Sociedad Argentina De Farmacología Experimental
Sociedad Argentina de Inmunologìa
Sociedad Argentina de Andrología
Sociedad Argentina de Biofísica
Sociedad Argentina de Biología
Sociedad Argentina de Hematología
description The increment of the global population and subsequently the increasing demand of food has promoted the use of pesticides such as hexachlorocyclohexane (HCH). HCH is a chlorinated pesticide used to protect crops from vector borne diseases. Large-scale production and the extensive use of this compound led to deterioration of environmental quality owing to its persistence in the environment. Over the last years, bacteria-mediated degradation of toxic organic compounds has become in an effective biotechnological process. Streptomyces sp. M7 was isolated from polluted sediments in the province of Tucumán, Argentina. Several physiological studies demonstrated the ability of M7 to remove HCH and highlighted the potential of this strain to be used in bioremediation processes. Degradation pathways for HCH and organochlorine compounds in general, are unknown in Actinobacteria. So far, a mechanism for the degradation of HCH was only proposed in Sphingobium japonicum. MS-based proteomics have become a powerful tool to elucidate and understand the mechanisms that underlie physiological processes. In the present work we used a MS-based, label-free, and quantitative proteomic approach as a starting point for understanding the degradation pathway for HCH in our strain. M7 proteome showed 293 proteins that were significantly up-regulated in the presence of HCH. Key enzymes involved in the dechlorination of HCH (LinA; LinB and LinC) were identified. In addition, 8 proteins assigned to the xenobiotic degradation category could be involved in the so called downstream degradation pathway of HCH. Proteomic results support the physiological capacity of Streptomyces sp. M7 to degrade hexachlorocyclohexane. In turn, the obtained results will allow to postulate a degradative pathway for this bacterium.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195120
Label-free proteomic analysis of the hexachlorocyclohexane-degrading actinobacteria Streptomyces sp. M7; LXII Reunión Anual de la Sociedad Argentina De Investigación Clínica; LXV Reunión Anual de la Sociedad Argentina De Inmunología; XLIX Reunión Anual de la Sociedad Argentina De Farmacología Experimental; LIII Reunión Anual de la Sociedad Argentina De Investigación Bioquímica Y Biología Molecular; Reunión Anual de la Sociedad Argentina De Fisiología y XXIX Reunión Anual de la Sociedad Argentina De Protozoología; Ciudad Autónoma de Buenos Aires; Argentina; 2017; 382-382
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195120
identifier_str_mv Label-free proteomic analysis of the hexachlorocyclohexane-degrading actinobacteria Streptomyces sp. M7; LXII Reunión Anual de la Sociedad Argentina De Investigación Clínica; LXV Reunión Anual de la Sociedad Argentina De Inmunología; XLIX Reunión Anual de la Sociedad Argentina De Farmacología Experimental; LIII Reunión Anual de la Sociedad Argentina De Investigación Bioquímica Y Biología Molecular; Reunión Anual de la Sociedad Argentina De Fisiología y XXIX Reunión Anual de la Sociedad Argentina De Protozoología; Ciudad Autónoma de Buenos Aires; Argentina; 2017; 382-382
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol77-17/Vol.77SuplementoI-2017.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981162533781504
score 12.48226

Publicaciones similares