Production of white, red and black quinoa (Chenopodium quinoa willd var. real) protein isolates and its hydrolysates in germinated and non-germinated quinoa samples and antioxidant...

Autores
Piñuel, Maria Lucrecia; Boeri, Patricia; Zubillaga, Fanny; Barrio, Daniel Alejandro; Torreta, Joaquin; Cruz, Andrea; Vásquez, Grace; Pinto, Adelita; Carrillo, Wilman
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Red, black and white seeds quinoa were germinated at 28◦ C during 24 (G1), 48 and 72 h (G3). Red quinoa presented a higher percentage of germination with a value of 46% of germination at 72 h. Quinoa protein isolate (QPI) was obtained by alkaline extraction (pH 8.0) followed by an isoelectric precipitation (pH 4.5) from white, red and black quinoa seeds, germinated QPI-G1 or QPI-G3 and non-germinated QPI-NG, Chenopodium quinoa Willd var. Real. QPI-G1, QPI-G3 and QPI-NG were subject to a simulated gastric digestion (DG) and in vitro duodenal digestion (DD). The antioxidant activity was evaluated using the 1, 1-diphenyl-2-picryl hydrazyl (DPPH), azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC) methods. Gastric and duodenal digest of QPI-NG and QPI-G1 and QPI-G3 from white, red and black quinoa presented antioxidant activity. QPI-G1-DD of white quinoa presented the highest antioxidant activity with a DPPH value of 167.98 µmoL TE/g of digest, QPI-G1-DD of red quinoa with an ABTS value of 204.86 µmoL TE/g of digest and QPI-G1-DD of black quinoa with an ORAC value of 401.42 µmoL TE/g of digest. QPI-G3-DD of white quinoa presented higher antioxidant activity with a DPPH value of 186.28 µmoL TE/g of sample, QPI-G3-DD of red quinoa with an ABTS value of 144.06 µmoL TE/g of digest and QPI-G3-DD of black quinoa with an ORAC value of 395.14 µmoL TE/g of digest. The inhibition of reactive oxygen species (ROS) production in the zebrafish embryo model (Danio rerio) was evaluated. Protein profiles of QPI from white, red and black from germinated quinoa and non-germinated quinoa were similar with proteins between 10 kDa to 100 kDa with the presence of globulins 11S and 7S and 2S albumins.
Fil: Piñuel, Maria Lucrecia. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Boeri, Patricia. Universidad Técnica de Babahoyo; Ecuador
Fil: Zubillaga, Fanny. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Barrio, Daniel Alejandro. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Torreta, Joaquin. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Cruz, Andrea. Escuela Superior Politécnica del Litoral; Ecuador
Fil: Vásquez, Grace. Escuela Superior Politécnica del Litoral; Ecuador
Fil: Pinto, Adelita. Universidad Técnica de Babahoyo; Ecuador
Fil: Carrillo, Wilman. Universidad Técnica de Babahoyo; Ecuador
Materia
ANTIOXIDANT ACTIVITY
HYDROLYSATES
QUINOA
QUINOA PROTEIN CONCENTRATES
ZEBRAFISH EMBRYOS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/132513
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/132513
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Production of white, red and black quinoa (Chenopodium quinoa willd var. real) protein isolates and its hydrolysates in germinated and non-germinated quinoa samples and antioxidant activity evaluationPiñuel, Maria LucreciaBoeri, PatriciaZubillaga, FannyBarrio, Daniel AlejandroTorreta, JoaquinCruz, AndreaVásquez, GracePinto, AdelitaCarrillo, WilmanANTIOXIDANT ACTIVITYHYDROLYSATESQUINOAQUINOA PROTEIN CONCENTRATESZEBRAFISH EMBRYOShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Red, black and white seeds quinoa were germinated at 28◦ C during 24 (G1), 48 and 72 h (G3). Red quinoa presented a higher percentage of germination with a value of 46% of germination at 72 h. Quinoa protein isolate (QPI) was obtained by alkaline extraction (pH 8.0) followed by an isoelectric precipitation (pH 4.5) from white, red and black quinoa seeds, germinated QPI-G1 or QPI-G3 and non-germinated QPI-NG, Chenopodium quinoa Willd var. Real. QPI-G1, QPI-G3 and QPI-NG were subject to a simulated gastric digestion (DG) and in vitro duodenal digestion (DD). The antioxidant activity was evaluated using the 1, 1-diphenyl-2-picryl hydrazyl (DPPH), azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC) methods. Gastric and duodenal digest of QPI-NG and QPI-G1 and QPI-G3 from white, red and black quinoa presented antioxidant activity. QPI-G1-DD of white quinoa presented the highest antioxidant activity with a DPPH value of 167.98 µmoL TE/g of digest, QPI-G1-DD of red quinoa with an ABTS value of 204.86 µmoL TE/g of digest and QPI-G1-DD of black quinoa with an ORAC value of 401.42 µmoL TE/g of digest. QPI-G3-DD of white quinoa presented higher antioxidant activity with a DPPH value of 186.28 µmoL TE/g of sample, QPI-G3-DD of red quinoa with an ABTS value of 144.06 µmoL TE/g of digest and QPI-G3-DD of black quinoa with an ORAC value of 395.14 µmoL TE/g of digest. The inhibition of reactive oxygen species (ROS) production in the zebrafish embryo model (Danio rerio) was evaluated. Protein profiles of QPI from white, red and black from germinated quinoa and non-germinated quinoa were similar with proteins between 10 kDa to 100 kDa with the presence of globulins 11S and 7S and 2S albumins.Fil: Piñuel, Maria Lucrecia. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; ArgentinaFil: Boeri, Patricia. Universidad Técnica de Babahoyo; EcuadorFil: Zubillaga, Fanny. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; ArgentinaFil: Barrio, Daniel Alejandro. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; ArgentinaFil: Torreta, Joaquin. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; ArgentinaFil: Cruz, Andrea. Escuela Superior Politécnica del Litoral; EcuadorFil: Vásquez, Grace. Escuela Superior Politécnica del Litoral; EcuadorFil: Pinto, Adelita. Universidad Técnica de Babahoyo; EcuadorFil: Carrillo, Wilman. Universidad Técnica de Babahoyo; EcuadorMultidisciplinary Digital Publishing Institute2019-07-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/132513Piñuel, Maria Lucrecia; Boeri, Patricia; Zubillaga, Fanny; Barrio, Daniel Alejandro; Torreta, Joaquin; et al.; Production of white, red and black quinoa (Chenopodium quinoa willd var. real) protein isolates and its hydrolysates in germinated and non-germinated quinoa samples and antioxidant activity evaluation; Multidisciplinary Digital Publishing Institute; Plants; 8; 8; 30-7-2019; 1-182223-7747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/plants8080257info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/8/8/257info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:10:02Zoai:ri.conicet.gov.ar:11336/132513instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:10:02.555CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Production of white, red and black quinoa (Chenopodium quinoa willd var. real) protein isolates and its hydrolysates in germinated and non-germinated quinoa samples and antioxidant activity evaluation
title Production of white, red and black quinoa (Chenopodium quinoa willd var. real) protein isolates and its hydrolysates in germinated and non-germinated quinoa samples and antioxidant activity evaluation
spellingShingle Production of white, red and black quinoa (Chenopodium quinoa willd var. real) protein isolates and its hydrolysates in germinated and non-germinated quinoa samples and antioxidant activity evaluation
Piñuel, Maria Lucrecia
ANTIOXIDANT ACTIVITY
HYDROLYSATES
QUINOA
QUINOA PROTEIN CONCENTRATES
ZEBRAFISH EMBRYOS
title_short Production of white, red and black quinoa (Chenopodium quinoa willd var. real) protein isolates and its hydrolysates in germinated and non-germinated quinoa samples and antioxidant activity evaluation
title_full Production of white, red and black quinoa (Chenopodium quinoa willd var. real) protein isolates and its hydrolysates in germinated and non-germinated quinoa samples and antioxidant activity evaluation
title_fullStr Production of white, red and black quinoa (Chenopodium quinoa willd var. real) protein isolates and its hydrolysates in germinated and non-germinated quinoa samples and antioxidant activity evaluation
title_full_unstemmed Production of white, red and black quinoa (Chenopodium quinoa willd var. real) protein isolates and its hydrolysates in germinated and non-germinated quinoa samples and antioxidant activity evaluation
title_sort Production of white, red and black quinoa (Chenopodium quinoa willd var. real) protein isolates and its hydrolysates in germinated and non-germinated quinoa samples and antioxidant activity evaluation
dc.creator.none.fl_str_mv Piñuel, Maria Lucrecia
Boeri, Patricia
Zubillaga, Fanny
Barrio, Daniel Alejandro
Torreta, Joaquin
Cruz, Andrea
Vásquez, Grace
Pinto, Adelita
Carrillo, Wilman
author Piñuel, Maria Lucrecia
author_facet Piñuel, Maria Lucrecia
Boeri, Patricia
Zubillaga, Fanny
Barrio, Daniel Alejandro
Torreta, Joaquin
Cruz, Andrea
Vásquez, Grace
Pinto, Adelita
Carrillo, Wilman
author_role author
author2 Boeri, Patricia
Zubillaga, Fanny
Barrio, Daniel Alejandro
Torreta, Joaquin
Cruz, Andrea
Vásquez, Grace
Pinto, Adelita
Carrillo, Wilman
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIOXIDANT ACTIVITY
HYDROLYSATES
QUINOA
QUINOA PROTEIN CONCENTRATES
ZEBRAFISH EMBRYOS
topic ANTIOXIDANT ACTIVITY
HYDROLYSATES
QUINOA
QUINOA PROTEIN CONCENTRATES
ZEBRAFISH EMBRYOS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Red, black and white seeds quinoa were germinated at 28◦ C during 24 (G1), 48 and 72 h (G3). Red quinoa presented a higher percentage of germination with a value of 46% of germination at 72 h. Quinoa protein isolate (QPI) was obtained by alkaline extraction (pH 8.0) followed by an isoelectric precipitation (pH 4.5) from white, red and black quinoa seeds, germinated QPI-G1 or QPI-G3 and non-germinated QPI-NG, Chenopodium quinoa Willd var. Real. QPI-G1, QPI-G3 and QPI-NG were subject to a simulated gastric digestion (DG) and in vitro duodenal digestion (DD). The antioxidant activity was evaluated using the 1, 1-diphenyl-2-picryl hydrazyl (DPPH), azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC) methods. Gastric and duodenal digest of QPI-NG and QPI-G1 and QPI-G3 from white, red and black quinoa presented antioxidant activity. QPI-G1-DD of white quinoa presented the highest antioxidant activity with a DPPH value of 167.98 µmoL TE/g of digest, QPI-G1-DD of red quinoa with an ABTS value of 204.86 µmoL TE/g of digest and QPI-G1-DD of black quinoa with an ORAC value of 401.42 µmoL TE/g of digest. QPI-G3-DD of white quinoa presented higher antioxidant activity with a DPPH value of 186.28 µmoL TE/g of sample, QPI-G3-DD of red quinoa with an ABTS value of 144.06 µmoL TE/g of digest and QPI-G3-DD of black quinoa with an ORAC value of 395.14 µmoL TE/g of digest. The inhibition of reactive oxygen species (ROS) production in the zebrafish embryo model (Danio rerio) was evaluated. Protein profiles of QPI from white, red and black from germinated quinoa and non-germinated quinoa were similar with proteins between 10 kDa to 100 kDa with the presence of globulins 11S and 7S and 2S albumins.
Fil: Piñuel, Maria Lucrecia. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Boeri, Patricia. Universidad Técnica de Babahoyo; Ecuador
Fil: Zubillaga, Fanny. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Barrio, Daniel Alejandro. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Torreta, Joaquin. Universidad Nacional de Rio Negro. Centro de Investigaciones y Transferencia de Rio Negro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Rio Negro; Argentina
Fil: Cruz, Andrea. Escuela Superior Politécnica del Litoral; Ecuador
Fil: Vásquez, Grace. Escuela Superior Politécnica del Litoral; Ecuador
Fil: Pinto, Adelita. Universidad Técnica de Babahoyo; Ecuador
Fil: Carrillo, Wilman. Universidad Técnica de Babahoyo; Ecuador
description Red, black and white seeds quinoa were germinated at 28◦ C during 24 (G1), 48 and 72 h (G3). Red quinoa presented a higher percentage of germination with a value of 46% of germination at 72 h. Quinoa protein isolate (QPI) was obtained by alkaline extraction (pH 8.0) followed by an isoelectric precipitation (pH 4.5) from white, red and black quinoa seeds, germinated QPI-G1 or QPI-G3 and non-germinated QPI-NG, Chenopodium quinoa Willd var. Real. QPI-G1, QPI-G3 and QPI-NG were subject to a simulated gastric digestion (DG) and in vitro duodenal digestion (DD). The antioxidant activity was evaluated using the 1, 1-diphenyl-2-picryl hydrazyl (DPPH), azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC) methods. Gastric and duodenal digest of QPI-NG and QPI-G1 and QPI-G3 from white, red and black quinoa presented antioxidant activity. QPI-G1-DD of white quinoa presented the highest antioxidant activity with a DPPH value of 167.98 µmoL TE/g of digest, QPI-G1-DD of red quinoa with an ABTS value of 204.86 µmoL TE/g of digest and QPI-G1-DD of black quinoa with an ORAC value of 401.42 µmoL TE/g of digest. QPI-G3-DD of white quinoa presented higher antioxidant activity with a DPPH value of 186.28 µmoL TE/g of sample, QPI-G3-DD of red quinoa with an ABTS value of 144.06 µmoL TE/g of digest and QPI-G3-DD of black quinoa with an ORAC value of 395.14 µmoL TE/g of digest. The inhibition of reactive oxygen species (ROS) production in the zebrafish embryo model (Danio rerio) was evaluated. Protein profiles of QPI from white, red and black from germinated quinoa and non-germinated quinoa were similar with proteins between 10 kDa to 100 kDa with the presence of globulins 11S and 7S and 2S albumins.
publishDate 2019
dc.date.none.fl_str_mv 2019-07-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/132513
Piñuel, Maria Lucrecia; Boeri, Patricia; Zubillaga, Fanny; Barrio, Daniel Alejandro; Torreta, Joaquin; et al.; Production of white, red and black quinoa (Chenopodium quinoa willd var. real) protein isolates and its hydrolysates in germinated and non-germinated quinoa samples and antioxidant activity evaluation; Multidisciplinary Digital Publishing Institute; Plants; 8; 8; 30-7-2019; 1-18
2223-7747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/132513
identifier_str_mv Piñuel, Maria Lucrecia; Boeri, Patricia; Zubillaga, Fanny; Barrio, Daniel Alejandro; Torreta, Joaquin; et al.; Production of white, red and black quinoa (Chenopodium quinoa willd var. real) protein isolates and its hydrolysates in germinated and non-germinated quinoa samples and antioxidant activity evaluation; Multidisciplinary Digital Publishing Institute; Plants; 8; 8; 30-7-2019; 1-18
2223-7747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/plants8080257
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/8/8/257
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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