Glial sPLA2-IID is associated with resolution responses during neurotoxicity.

Autores
Benzi Juncos, Oriana Nicole; Alza, Natalia Paola; Salvador, Gabriela Alejandra
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Resolution responses are associated with the metabolization of arachidonic acid (AA) and docosahexaenoic acid (DHA) by lipoxygenases. AA and DHA are released from phospholipids by phospholipases A2 (PLA2), to act as precursors of specialized pro-resolving lipid mediators (SPM). It is assumed that glial cells participate in both inflammation and resolution responses. The latter, mediated by SPM, counteract inflammatory responses. In this work, we used the pesticide maneb (MB), a well-described neurotoxicant, to study the role of glial cells in the resolution responses. We first characterize glial activation and related signaling pathways triggered by MB toxicity. Astrocytes and primary glial cultures displayed increased GFAP and S100β expression along with a greater nuclear translocation of the transcription factor NFκB. Also, astrocytes showed increased expression of Emp1, an A2 astrocyte marker, concomitantly with a decrease of the A1 marker, C3. In addition, microglia displayed increased expression of IBA-1 and decreased Arg-1 mRNA levels associated with a switch to an enlarged amoeboid flattened morphology. However, the cytokine profile showed increased interleukin-1β expression. To further explore the role of astrocytes and neurons in resolution, fatty acid profiling was assessed by GC-MS. Neurons presented increased DHA content while astrocytes displayed decreased AA and DHA levels. The respective secretomes exhibited an inverse profile, with increased DHA in the astrocytic secretome and decreased AA and DHA in the neuronal medium. In light of these findings, we analyzed Pla2g2d expression, a secretory PLA2 known for its anti-inflammatory role in immune cells. MB differentially affected Pla2g2d expression by promoting its upregulation in neurons and the downregulation in astrocytes. Concomitantly, the secretome of MB-exposed neurons restored Pla2g2d expression levels in astrocytes to control levels. In contrast, the secretome from astrocytes exposed to the pesticide downregulated Pla2g2d expression, indicating that when neurons received DHA exogenously, the enzyme was downregulated. In addition, the inhibition of secretory PLA2 prevented the astrocytic proliferative effect elicited by neurons. Our results argue in favor of the relevance of resolution pathways involving sPLA2-IID acting as a modulator of DHA levels in the glial response against the pesticide
Fil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Córdoba
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Materia
Phospholipase A2
Neurotoxicity
Resolution
Neuron-glia crosstalk
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/278763
Acceder
id CONICETDig_c8a4430fb5f0ad6e92d8f6c7c87aeeda
oai_identifier_str oai:ri.conicet.gov.ar:11336/278763
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Glial sPLA2-IID is associated with resolution responses during neurotoxicity.Benzi Juncos, Oriana NicoleAlza, Natalia PaolaSalvador, Gabriela AlejandraPhospholipase A2NeurotoxicityResolutionNeuron-glia crosstalkhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Resolution responses are associated with the metabolization of arachidonic acid (AA) and docosahexaenoic acid (DHA) by lipoxygenases. AA and DHA are released from phospholipids by phospholipases A2 (PLA2), to act as precursors of specialized pro-resolving lipid mediators (SPM). It is assumed that glial cells participate in both inflammation and resolution responses. The latter, mediated by SPM, counteract inflammatory responses. In this work, we used the pesticide maneb (MB), a well-described neurotoxicant, to study the role of glial cells in the resolution responses. We first characterize glial activation and related signaling pathways triggered by MB toxicity. Astrocytes and primary glial cultures displayed increased GFAP and S100β expression along with a greater nuclear translocation of the transcription factor NFκB. Also, astrocytes showed increased expression of Emp1, an A2 astrocyte marker, concomitantly with a decrease of the A1 marker, C3. In addition, microglia displayed increased expression of IBA-1 and decreased Arg-1 mRNA levels associated with a switch to an enlarged amoeboid flattened morphology. However, the cytokine profile showed increased interleukin-1β expression. To further explore the role of astrocytes and neurons in resolution, fatty acid profiling was assessed by GC-MS. Neurons presented increased DHA content while astrocytes displayed decreased AA and DHA levels. The respective secretomes exhibited an inverse profile, with increased DHA in the astrocytic secretome and decreased AA and DHA in the neuronal medium. In light of these findings, we analyzed Pla2g2d expression, a secretory PLA2 known for its anti-inflammatory role in immune cells. MB differentially affected Pla2g2d expression by promoting its upregulation in neurons and the downregulation in astrocytes. Concomitantly, the secretome of MB-exposed neurons restored Pla2g2d expression levels in astrocytes to control levels. In contrast, the secretome from astrocytes exposed to the pesticide downregulated Pla2g2d expression, indicating that when neurons received DHA exogenously, the enzyme was downregulated. In addition, the inhibition of secretory PLA2 prevented the astrocytic proliferative effect elicited by neurons. Our results argue in favor of the relevance of resolution pathways involving sPLA2-IID acting as a modulator of DHA levels in the glial response against the pesticideFil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology ResearchCórdobaArgentinaSociedad Argentina de Investigaciones en Bioquímica y Biología MolecularSociedad Argentina de Investigaciones en Bioquímica y Biología Molecular2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/278763Glial sPLA2-IID is associated with resolution responses during neurotoxicity.; LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Córdoba; Argentina; 2024; 46-47CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/2024/abstracts-EN.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-06T13:04:34Zoai:ri.conicet.gov.ar:11336/278763instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-06 13:04:34.881CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Glial sPLA2-IID is associated with resolution responses during neurotoxicity.
title Glial sPLA2-IID is associated with resolution responses during neurotoxicity.
spellingShingle Glial sPLA2-IID is associated with resolution responses during neurotoxicity.
Benzi Juncos, Oriana Nicole
Phospholipase A2
Neurotoxicity
Resolution
Neuron-glia crosstalk
title_short Glial sPLA2-IID is associated with resolution responses during neurotoxicity.
title_full Glial sPLA2-IID is associated with resolution responses during neurotoxicity.
title_fullStr Glial sPLA2-IID is associated with resolution responses during neurotoxicity.
title_full_unstemmed Glial sPLA2-IID is associated with resolution responses during neurotoxicity.
title_sort Glial sPLA2-IID is associated with resolution responses during neurotoxicity.
dc.creator.none.fl_str_mv Benzi Juncos, Oriana Nicole
Alza, Natalia Paola
Salvador, Gabriela Alejandra
author Benzi Juncos, Oriana Nicole
author_facet Benzi Juncos, Oriana Nicole
Alza, Natalia Paola
Salvador, Gabriela Alejandra
author_role author
author2 Alza, Natalia Paola
Salvador, Gabriela Alejandra
author2_role author
author
dc.subject.none.fl_str_mv Phospholipase A2
Neurotoxicity
Resolution
Neuron-glia crosstalk
topic Phospholipase A2
Neurotoxicity
Resolution
Neuron-glia crosstalk
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Resolution responses are associated with the metabolization of arachidonic acid (AA) and docosahexaenoic acid (DHA) by lipoxygenases. AA and DHA are released from phospholipids by phospholipases A2 (PLA2), to act as precursors of specialized pro-resolving lipid mediators (SPM). It is assumed that glial cells participate in both inflammation and resolution responses. The latter, mediated by SPM, counteract inflammatory responses. In this work, we used the pesticide maneb (MB), a well-described neurotoxicant, to study the role of glial cells in the resolution responses. We first characterize glial activation and related signaling pathways triggered by MB toxicity. Astrocytes and primary glial cultures displayed increased GFAP and S100β expression along with a greater nuclear translocation of the transcription factor NFκB. Also, astrocytes showed increased expression of Emp1, an A2 astrocyte marker, concomitantly with a decrease of the A1 marker, C3. In addition, microglia displayed increased expression of IBA-1 and decreased Arg-1 mRNA levels associated with a switch to an enlarged amoeboid flattened morphology. However, the cytokine profile showed increased interleukin-1β expression. To further explore the role of astrocytes and neurons in resolution, fatty acid profiling was assessed by GC-MS. Neurons presented increased DHA content while astrocytes displayed decreased AA and DHA levels. The respective secretomes exhibited an inverse profile, with increased DHA in the astrocytic secretome and decreased AA and DHA in the neuronal medium. In light of these findings, we analyzed Pla2g2d expression, a secretory PLA2 known for its anti-inflammatory role in immune cells. MB differentially affected Pla2g2d expression by promoting its upregulation in neurons and the downregulation in astrocytes. Concomitantly, the secretome of MB-exposed neurons restored Pla2g2d expression levels in astrocytes to control levels. In contrast, the secretome from astrocytes exposed to the pesticide downregulated Pla2g2d expression, indicating that when neurons received DHA exogenously, the enzyme was downregulated. In addition, the inhibition of secretory PLA2 prevented the astrocytic proliferative effect elicited by neurons. Our results argue in favor of the relevance of resolution pathways involving sPLA2-IID acting as a modulator of DHA levels in the glial response against the pesticide
Fil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Córdoba
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
description Resolution responses are associated with the metabolization of arachidonic acid (AA) and docosahexaenoic acid (DHA) by lipoxygenases. AA and DHA are released from phospholipids by phospholipases A2 (PLA2), to act as precursors of specialized pro-resolving lipid mediators (SPM). It is assumed that glial cells participate in both inflammation and resolution responses. The latter, mediated by SPM, counteract inflammatory responses. In this work, we used the pesticide maneb (MB), a well-described neurotoxicant, to study the role of glial cells in the resolution responses. We first characterize glial activation and related signaling pathways triggered by MB toxicity. Astrocytes and primary glial cultures displayed increased GFAP and S100β expression along with a greater nuclear translocation of the transcription factor NFκB. Also, astrocytes showed increased expression of Emp1, an A2 astrocyte marker, concomitantly with a decrease of the A1 marker, C3. In addition, microglia displayed increased expression of IBA-1 and decreased Arg-1 mRNA levels associated with a switch to an enlarged amoeboid flattened morphology. However, the cytokine profile showed increased interleukin-1β expression. To further explore the role of astrocytes and neurons in resolution, fatty acid profiling was assessed by GC-MS. Neurons presented increased DHA content while astrocytes displayed decreased AA and DHA levels. The respective secretomes exhibited an inverse profile, with increased DHA in the astrocytic secretome and decreased AA and DHA in the neuronal medium. In light of these findings, we analyzed Pla2g2d expression, a secretory PLA2 known for its anti-inflammatory role in immune cells. MB differentially affected Pla2g2d expression by promoting its upregulation in neurons and the downregulation in astrocytes. Concomitantly, the secretome of MB-exposed neurons restored Pla2g2d expression levels in astrocytes to control levels. In contrast, the secretome from astrocytes exposed to the pesticide downregulated Pla2g2d expression, indicating that when neurons received DHA exogenously, the enzyme was downregulated. In addition, the inhibition of secretory PLA2 prevented the astrocytic proliferative effect elicited by neurons. Our results argue in favor of the relevance of resolution pathways involving sPLA2-IID acting as a modulator of DHA levels in the glial response against the pesticide
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/278763
Glial sPLA2-IID is associated with resolution responses during neurotoxicity.; LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Córdoba; Argentina; 2024; 46-47
CONICET Digital
CONICET
url http://hdl.handle.net/11336/278763
identifier_str_mv Glial sPLA2-IID is associated with resolution responses during neurotoxicity.; LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Córdoba; Argentina; 2024; 46-47
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/2024/abstracts-EN.pdf
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
application/pdf
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
publisher.none.fl_str_mv Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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