Multiple bHLH/MYB-based protein complexes regulate proanthocyanidin biosynthesis in the herbage of Lotus spp

Autores
Escaray, Francisco José; Valeri, Maria Cristina; Damiani, Francesco; Ruiz, Oscar Adolfo; Carrasco Sorli, Pedro; Paolocci, Francesco
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The environmental impact and health of ruminants fed with forage legumes depend on the herbage's concentration and structure of proanthocyanidins (PAs). Unfortunately, the primary forage legumes (alfalfa and clover) do not contain substantial levels of PAs. No signifcant progress has been made to induce PAs to agronomically valuable levels in their edible organs by biotechnological approaches thus far. Building this trait requires a profound knowledge of PA regulators and their interplay in species naturally committed to accumulating these metabolites in the target organs. Against this background, we compared the shoot transcriptomes of two inter-fertile Lotus species, namely Lotus tenuis and Lotus corniculatus, polymorphic for this trait, to search for diferentially expressed MYB and bHLH genes. We then tested the expression of the above-reported regulators in L. tenuis x L. corniculatus interspecifc hybrids, several Lotus spp., and diferent L. corniculatus organs with contrasting PA levels. We identifed a novel MYB activator and MYB-bHLH-based complexes that, when expressed in Nicotiana benthamiana, trans-activated the promoters of L. corniculatus anthocyanidin reductase and leucoanthocyanidin reductase 1 genes. The last are the two critical structural genes for the biosynthesis of PAs in Lotus spp. Competition between MYB activators for the transactivation of these promoters also emerged. Overall, by employing Lotus as a model genus, we refned the transcriptional network underlying PA biosynthesis in the herbage of legumes. These fndings are crucial to engineering this trait in pasture legumes.
Fil: Escaray, Francisco José. Universidad Politécnica de Valencia; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Superior de Investigaciones Científicas; España
Fil: Valeri, Maria Cristina. Consiglio Nazionale delle Ricerche; Italia
Fil: Damiani, Francesco. Consiglio Nazionale delle Ricerche; Italia
Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Carrasco Sorli, Pedro. Universidad de Valencia; España
Fil: Paolocci, Francesco. Consiglio Nazionale delle Ricerche; Italia
Materia
CONDENSED TANNINS
FORAGE BREEDING
RUMINAL BLOOTING
TRANSCRIPTOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/231180
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/231180
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Multiple bHLH/MYB-based protein complexes regulate proanthocyanidin biosynthesis in the herbage of Lotus sppEscaray, Francisco JoséValeri, Maria CristinaDamiani, FrancescoRuiz, Oscar AdolfoCarrasco Sorli, PedroPaolocci, FrancescoCONDENSED TANNINSFORAGE BREEDINGRUMINAL BLOOTINGTRANSCRIPTOMEhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The environmental impact and health of ruminants fed with forage legumes depend on the herbage's concentration and structure of proanthocyanidins (PAs). Unfortunately, the primary forage legumes (alfalfa and clover) do not contain substantial levels of PAs. No signifcant progress has been made to induce PAs to agronomically valuable levels in their edible organs by biotechnological approaches thus far. Building this trait requires a profound knowledge of PA regulators and their interplay in species naturally committed to accumulating these metabolites in the target organs. Against this background, we compared the shoot transcriptomes of two inter-fertile Lotus species, namely Lotus tenuis and Lotus corniculatus, polymorphic for this trait, to search for diferentially expressed MYB and bHLH genes. We then tested the expression of the above-reported regulators in L. tenuis x L. corniculatus interspecifc hybrids, several Lotus spp., and diferent L. corniculatus organs with contrasting PA levels. We identifed a novel MYB activator and MYB-bHLH-based complexes that, when expressed in Nicotiana benthamiana, trans-activated the promoters of L. corniculatus anthocyanidin reductase and leucoanthocyanidin reductase 1 genes. The last are the two critical structural genes for the biosynthesis of PAs in Lotus spp. Competition between MYB activators for the transactivation of these promoters also emerged. Overall, by employing Lotus as a model genus, we refned the transcriptional network underlying PA biosynthesis in the herbage of legumes. These fndings are crucial to engineering this trait in pasture legumes.Fil: Escaray, Francisco José. Universidad Politécnica de Valencia; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Superior de Investigaciones Científicas; EspañaFil: Valeri, Maria Cristina. Consiglio Nazionale delle Ricerche; ItaliaFil: Damiani, Francesco. Consiglio Nazionale delle Ricerche; ItaliaFil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Carrasco Sorli, Pedro. Universidad de Valencia; EspañaFil: Paolocci, Francesco. Consiglio Nazionale delle Ricerche; ItaliaSpringer2024-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/231180Escaray, Francisco José; Valeri, Maria Cristina; Damiani, Francesco; Ruiz, Oscar Adolfo; Carrasco Sorli, Pedro; et al.; Multiple bHLH/MYB-based protein complexes regulate proanthocyanidin biosynthesis in the herbage of Lotus spp; Springer; Planta; 259; 10; 1-2024; 1-160032-0935CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00425-023-04281-2info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00425-023-04281-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:17:40Zoai:ri.conicet.gov.ar:11336/231180instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:17:41.296CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multiple bHLH/MYB-based protein complexes regulate proanthocyanidin biosynthesis in the herbage of Lotus spp
title Multiple bHLH/MYB-based protein complexes regulate proanthocyanidin biosynthesis in the herbage of Lotus spp
spellingShingle Multiple bHLH/MYB-based protein complexes regulate proanthocyanidin biosynthesis in the herbage of Lotus spp
Escaray, Francisco José
CONDENSED TANNINS
FORAGE BREEDING
RUMINAL BLOOTING
TRANSCRIPTOME
title_short Multiple bHLH/MYB-based protein complexes regulate proanthocyanidin biosynthesis in the herbage of Lotus spp
title_full Multiple bHLH/MYB-based protein complexes regulate proanthocyanidin biosynthesis in the herbage of Lotus spp
title_fullStr Multiple bHLH/MYB-based protein complexes regulate proanthocyanidin biosynthesis in the herbage of Lotus spp
title_full_unstemmed Multiple bHLH/MYB-based protein complexes regulate proanthocyanidin biosynthesis in the herbage of Lotus spp
title_sort Multiple bHLH/MYB-based protein complexes regulate proanthocyanidin biosynthesis in the herbage of Lotus spp
dc.creator.none.fl_str_mv Escaray, Francisco José
Valeri, Maria Cristina
Damiani, Francesco
Ruiz, Oscar Adolfo
Carrasco Sorli, Pedro
Paolocci, Francesco
author Escaray, Francisco José
author_facet Escaray, Francisco José
Valeri, Maria Cristina
Damiani, Francesco
Ruiz, Oscar Adolfo
Carrasco Sorli, Pedro
Paolocci, Francesco
author_role author
author2 Valeri, Maria Cristina
Damiani, Francesco
Ruiz, Oscar Adolfo
Carrasco Sorli, Pedro
Paolocci, Francesco
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CONDENSED TANNINS
FORAGE BREEDING
RUMINAL BLOOTING
TRANSCRIPTOME
topic CONDENSED TANNINS
FORAGE BREEDING
RUMINAL BLOOTING
TRANSCRIPTOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The environmental impact and health of ruminants fed with forage legumes depend on the herbage's concentration and structure of proanthocyanidins (PAs). Unfortunately, the primary forage legumes (alfalfa and clover) do not contain substantial levels of PAs. No signifcant progress has been made to induce PAs to agronomically valuable levels in their edible organs by biotechnological approaches thus far. Building this trait requires a profound knowledge of PA regulators and their interplay in species naturally committed to accumulating these metabolites in the target organs. Against this background, we compared the shoot transcriptomes of two inter-fertile Lotus species, namely Lotus tenuis and Lotus corniculatus, polymorphic for this trait, to search for diferentially expressed MYB and bHLH genes. We then tested the expression of the above-reported regulators in L. tenuis x L. corniculatus interspecifc hybrids, several Lotus spp., and diferent L. corniculatus organs with contrasting PA levels. We identifed a novel MYB activator and MYB-bHLH-based complexes that, when expressed in Nicotiana benthamiana, trans-activated the promoters of L. corniculatus anthocyanidin reductase and leucoanthocyanidin reductase 1 genes. The last are the two critical structural genes for the biosynthesis of PAs in Lotus spp. Competition between MYB activators for the transactivation of these promoters also emerged. Overall, by employing Lotus as a model genus, we refned the transcriptional network underlying PA biosynthesis in the herbage of legumes. These fndings are crucial to engineering this trait in pasture legumes.
Fil: Escaray, Francisco José. Universidad Politécnica de Valencia; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Superior de Investigaciones Científicas; España
Fil: Valeri, Maria Cristina. Consiglio Nazionale delle Ricerche; Italia
Fil: Damiani, Francesco. Consiglio Nazionale delle Ricerche; Italia
Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Carrasco Sorli, Pedro. Universidad de Valencia; España
Fil: Paolocci, Francesco. Consiglio Nazionale delle Ricerche; Italia
description The environmental impact and health of ruminants fed with forage legumes depend on the herbage's concentration and structure of proanthocyanidins (PAs). Unfortunately, the primary forage legumes (alfalfa and clover) do not contain substantial levels of PAs. No signifcant progress has been made to induce PAs to agronomically valuable levels in their edible organs by biotechnological approaches thus far. Building this trait requires a profound knowledge of PA regulators and their interplay in species naturally committed to accumulating these metabolites in the target organs. Against this background, we compared the shoot transcriptomes of two inter-fertile Lotus species, namely Lotus tenuis and Lotus corniculatus, polymorphic for this trait, to search for diferentially expressed MYB and bHLH genes. We then tested the expression of the above-reported regulators in L. tenuis x L. corniculatus interspecifc hybrids, several Lotus spp., and diferent L. corniculatus organs with contrasting PA levels. We identifed a novel MYB activator and MYB-bHLH-based complexes that, when expressed in Nicotiana benthamiana, trans-activated the promoters of L. corniculatus anthocyanidin reductase and leucoanthocyanidin reductase 1 genes. The last are the two critical structural genes for the biosynthesis of PAs in Lotus spp. Competition between MYB activators for the transactivation of these promoters also emerged. Overall, by employing Lotus as a model genus, we refned the transcriptional network underlying PA biosynthesis in the herbage of legumes. These fndings are crucial to engineering this trait in pasture legumes.
publishDate 2024
dc.date.none.fl_str_mv 2024-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/231180
Escaray, Francisco José; Valeri, Maria Cristina; Damiani, Francesco; Ruiz, Oscar Adolfo; Carrasco Sorli, Pedro; et al.; Multiple bHLH/MYB-based protein complexes regulate proanthocyanidin biosynthesis in the herbage of Lotus spp; Springer; Planta; 259; 10; 1-2024; 1-16
0032-0935
CONICET Digital
CONICET
url http://hdl.handle.net/11336/231180
identifier_str_mv Escaray, Francisco José; Valeri, Maria Cristina; Damiani, Francesco; Ruiz, Oscar Adolfo; Carrasco Sorli, Pedro; et al.; Multiple bHLH/MYB-based protein complexes regulate proanthocyanidin biosynthesis in the herbage of Lotus spp; Springer; Planta; 259; 10; 1-2024; 1-16
0032-0935
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00425-023-04281-2
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00425-023-04281-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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