Toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system: Finding new anti-inf...

Autores
Fujie, Hitomi; Villena, Julio Cesar; Tohno, Masanori; Morie, Kyoko; Shimazu, Tomoyuki; Aso, Hisashi; Suda, Yoshihito; Shimosato, Takeshi; Iwabuchi, Noriyuki; Xiao, Jin-Zhong; Yaeshima, Tomoko; Iwatsuki, Keiji; Saito, Tadao; Numasaki, Muneo; Kitazawa, Haruki
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A total of 23 strains of bifidobacteria taxonomically belonging to five species were tested for their potent immunomodulatory effect using a combination of two methods: the NF-κB-reporter assay using a toll-like receptor 2-expressing transfectant (HEK pTLR2 system) and the mitogenic assay using porcine Peyer's patches immunocompetent cells. Among the four preselected strains from different immunomodulatory groups, Bifidobacterium breve MCC-117 was able to efficiently modulate the inflammatory response triggered by enterotoxigenic Escherichia coli (ETEC) in a porcine intestinal epithelial (PIE) cell line. Moreover, using PIE cells and swine Peyer's patches immunocompetent cell co-culture system, we demonstrated that the immunoregulatory effect of B. breve MCC-117 was related to the capacity of the strain to influence PIE and immune cell interactions, leading to the stimulation of regulatory T cells. The results suggested that bifidobacteria that express high activity in both the HEK pTLR2 and the mitogenic assays may behave like potential anti-inflammatory strains. The combination of the HEK pTLR2 system, the evaluation of mitogenic activity and PIE cells will be of value for the development of new immunologically functional foods and feeds that could prevent inflammatory intestinal disorders. Although our findings should be proven in appropriate experiments in vivo, the results of the present work provide a scientific rationale for the use of B. breve MCC-117 to prevent ETEC-induced intestinal inflammation.
Fil: Fujie, Hitomi. Tohoku University; Japón
Fil: Villena, Julio Cesar. Tohoku University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Tohno, Masanori. National Agricultural Research Organization. National Institute of Livestock and Grassland Science. Functional Feed Research Team; Japón
Fil: Morie, Kyoko. Tohoku University; Japón
Fil: Shimazu, Tomoyuki. Tohoku University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Shimosato, Takeshi. Shinshu University; Japón
Fil: Iwabuchi, Noriyuki. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; Japón
Fil: Xiao, Jin-Zhong. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; Japón
Fil: Yaeshima, Tomoko. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; Japón
Fil: Iwatsuki, Keiji. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; Japón
Fil: Saito, Tadao. Tohoku University; Japón
Fil: Numasaki, Muneo. Josai University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Materia
Anti-Inflammatory Bifidobacteria
Intestinal Inflammation
Porcine Intestinal Epithelila Cells
Tlr-2 Activation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/57452
Acceder
id CONICETDig_c847be98373b61213ed69f0057416179
oai_identifier_str oai:ri.conicet.gov.ar:11336/57452
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system: Finding new anti-inflammatory immunobioticsFujie, HitomiVillena, Julio CesarTohno, MasanoriMorie, KyokoShimazu, TomoyukiAso, HisashiSuda, YoshihitoShimosato, TakeshiIwabuchi, NoriyukiXiao, Jin-ZhongYaeshima, TomokoIwatsuki, KeijiSaito, TadaoNumasaki, MuneoKitazawa, HarukiAnti-Inflammatory BifidobacteriaIntestinal InflammationPorcine Intestinal Epithelila CellsTlr-2 Activationhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4A total of 23 strains of bifidobacteria taxonomically belonging to five species were tested for their potent immunomodulatory effect using a combination of two methods: the NF-κB-reporter assay using a toll-like receptor 2-expressing transfectant (HEK pTLR2 system) and the mitogenic assay using porcine Peyer's patches immunocompetent cells. Among the four preselected strains from different immunomodulatory groups, Bifidobacterium breve MCC-117 was able to efficiently modulate the inflammatory response triggered by enterotoxigenic Escherichia coli (ETEC) in a porcine intestinal epithelial (PIE) cell line. Moreover, using PIE cells and swine Peyer's patches immunocompetent cell co-culture system, we demonstrated that the immunoregulatory effect of B. breve MCC-117 was related to the capacity of the strain to influence PIE and immune cell interactions, leading to the stimulation of regulatory T cells. The results suggested that bifidobacteria that express high activity in both the HEK pTLR2 and the mitogenic assays may behave like potential anti-inflammatory strains. The combination of the HEK pTLR2 system, the evaluation of mitogenic activity and PIE cells will be of value for the development of new immunologically functional foods and feeds that could prevent inflammatory intestinal disorders. Although our findings should be proven in appropriate experiments in vivo, the results of the present work provide a scientific rationale for the use of B. breve MCC-117 to prevent ETEC-induced intestinal inflammation.Fil: Fujie, Hitomi. Tohoku University; JapónFil: Villena, Julio Cesar. Tohoku University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Tohno, Masanori. National Agricultural Research Organization. National Institute of Livestock and Grassland Science. Functional Feed Research Team; JapónFil: Morie, Kyoko. Tohoku University; JapónFil: Shimazu, Tomoyuki. Tohoku University; JapónFil: Aso, Hisashi. Tohoku University; JapónFil: Suda, Yoshihito. Miyagi University; JapónFil: Shimosato, Takeshi. Shinshu University; JapónFil: Iwabuchi, Noriyuki. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; JapónFil: Xiao, Jin-Zhong. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; JapónFil: Yaeshima, Tomoko. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; JapónFil: Iwatsuki, Keiji. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; JapónFil: Saito, Tadao. Tohoku University; JapónFil: Numasaki, Muneo. Josai University; JapónFil: Kitazawa, Haruki. Tohoku University; JapónWiley Blackwell Publishing, Inc2011-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/57452Fujie, Hitomi; Villena, Julio Cesar; Tohno, Masanori; Morie, Kyoko; Shimazu, Tomoyuki; et al.; Toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system: Finding new anti-inflammatory immunobiotics; Wiley Blackwell Publishing, Inc; Fems Immunology And Medical Microbiology; 63; 1; 1-10-2011; 129-1390928-82441574-695XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-695X.2011.00837.xinfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femspd/article/63/1/129/549169info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:46:42Zoai:ri.conicet.gov.ar:11336/57452instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:46:42.657CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system: Finding new anti-inflammatory immunobiotics
title Toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system: Finding new anti-inflammatory immunobiotics
spellingShingle Toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system: Finding new anti-inflammatory immunobiotics
Fujie, Hitomi
Anti-Inflammatory Bifidobacteria
Intestinal Inflammation
Porcine Intestinal Epithelila Cells
Tlr-2 Activation
title_short Toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system: Finding new anti-inflammatory immunobiotics
title_full Toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system: Finding new anti-inflammatory immunobiotics
title_fullStr Toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system: Finding new anti-inflammatory immunobiotics
title_full_unstemmed Toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system: Finding new anti-inflammatory immunobiotics
title_sort Toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system: Finding new anti-inflammatory immunobiotics
dc.creator.none.fl_str_mv Fujie, Hitomi
Villena, Julio Cesar
Tohno, Masanori
Morie, Kyoko
Shimazu, Tomoyuki
Aso, Hisashi
Suda, Yoshihito
Shimosato, Takeshi
Iwabuchi, Noriyuki
Xiao, Jin-Zhong
Yaeshima, Tomoko
Iwatsuki, Keiji
Saito, Tadao
Numasaki, Muneo
Kitazawa, Haruki
author Fujie, Hitomi
author_facet Fujie, Hitomi
Villena, Julio Cesar
Tohno, Masanori
Morie, Kyoko
Shimazu, Tomoyuki
Aso, Hisashi
Suda, Yoshihito
Shimosato, Takeshi
Iwabuchi, Noriyuki
Xiao, Jin-Zhong
Yaeshima, Tomoko
Iwatsuki, Keiji
Saito, Tadao
Numasaki, Muneo
Kitazawa, Haruki
author_role author
author2 Villena, Julio Cesar
Tohno, Masanori
Morie, Kyoko
Shimazu, Tomoyuki
Aso, Hisashi
Suda, Yoshihito
Shimosato, Takeshi
Iwabuchi, Noriyuki
Xiao, Jin-Zhong
Yaeshima, Tomoko
Iwatsuki, Keiji
Saito, Tadao
Numasaki, Muneo
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anti-Inflammatory Bifidobacteria
Intestinal Inflammation
Porcine Intestinal Epithelila Cells
Tlr-2 Activation
topic Anti-Inflammatory Bifidobacteria
Intestinal Inflammation
Porcine Intestinal Epithelila Cells
Tlr-2 Activation
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv A total of 23 strains of bifidobacteria taxonomically belonging to five species were tested for their potent immunomodulatory effect using a combination of two methods: the NF-κB-reporter assay using a toll-like receptor 2-expressing transfectant (HEK pTLR2 system) and the mitogenic assay using porcine Peyer's patches immunocompetent cells. Among the four preselected strains from different immunomodulatory groups, Bifidobacterium breve MCC-117 was able to efficiently modulate the inflammatory response triggered by enterotoxigenic Escherichia coli (ETEC) in a porcine intestinal epithelial (PIE) cell line. Moreover, using PIE cells and swine Peyer's patches immunocompetent cell co-culture system, we demonstrated that the immunoregulatory effect of B. breve MCC-117 was related to the capacity of the strain to influence PIE and immune cell interactions, leading to the stimulation of regulatory T cells. The results suggested that bifidobacteria that express high activity in both the HEK pTLR2 and the mitogenic assays may behave like potential anti-inflammatory strains. The combination of the HEK pTLR2 system, the evaluation of mitogenic activity and PIE cells will be of value for the development of new immunologically functional foods and feeds that could prevent inflammatory intestinal disorders. Although our findings should be proven in appropriate experiments in vivo, the results of the present work provide a scientific rationale for the use of B. breve MCC-117 to prevent ETEC-induced intestinal inflammation.
Fil: Fujie, Hitomi. Tohoku University; Japón
Fil: Villena, Julio Cesar. Tohoku University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Tohno, Masanori. National Agricultural Research Organization. National Institute of Livestock and Grassland Science. Functional Feed Research Team; Japón
Fil: Morie, Kyoko. Tohoku University; Japón
Fil: Shimazu, Tomoyuki. Tohoku University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Shimosato, Takeshi. Shinshu University; Japón
Fil: Iwabuchi, Noriyuki. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; Japón
Fil: Xiao, Jin-Zhong. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; Japón
Fil: Yaeshima, Tomoko. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; Japón
Fil: Iwatsuki, Keiji. Morinaga Milk Industry Co. Ltd. Food Science and Technology Institute; Japón
Fil: Saito, Tadao. Tohoku University; Japón
Fil: Numasaki, Muneo. Josai University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
description A total of 23 strains of bifidobacteria taxonomically belonging to five species were tested for their potent immunomodulatory effect using a combination of two methods: the NF-κB-reporter assay using a toll-like receptor 2-expressing transfectant (HEK pTLR2 system) and the mitogenic assay using porcine Peyer's patches immunocompetent cells. Among the four preselected strains from different immunomodulatory groups, Bifidobacterium breve MCC-117 was able to efficiently modulate the inflammatory response triggered by enterotoxigenic Escherichia coli (ETEC) in a porcine intestinal epithelial (PIE) cell line. Moreover, using PIE cells and swine Peyer's patches immunocompetent cell co-culture system, we demonstrated that the immunoregulatory effect of B. breve MCC-117 was related to the capacity of the strain to influence PIE and immune cell interactions, leading to the stimulation of regulatory T cells. The results suggested that bifidobacteria that express high activity in both the HEK pTLR2 and the mitogenic assays may behave like potential anti-inflammatory strains. The combination of the HEK pTLR2 system, the evaluation of mitogenic activity and PIE cells will be of value for the development of new immunologically functional foods and feeds that could prevent inflammatory intestinal disorders. Although our findings should be proven in appropriate experiments in vivo, the results of the present work provide a scientific rationale for the use of B. breve MCC-117 to prevent ETEC-induced intestinal inflammation.
publishDate 2011
dc.date.none.fl_str_mv 2011-10-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/57452
Fujie, Hitomi; Villena, Julio Cesar; Tohno, Masanori; Morie, Kyoko; Shimazu, Tomoyuki; et al.; Toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system: Finding new anti-inflammatory immunobiotics; Wiley Blackwell Publishing, Inc; Fems Immunology And Medical Microbiology; 63; 1; 1-10-2011; 129-139
0928-8244
1574-695X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/57452
identifier_str_mv Fujie, Hitomi; Villena, Julio Cesar; Tohno, Masanori; Morie, Kyoko; Shimazu, Tomoyuki; et al.; Toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system: Finding new anti-inflammatory immunobiotics; Wiley Blackwell Publishing, Inc; Fems Immunology And Medical Microbiology; 63; 1; 1-10-2011; 129-139
0928-8244
1574-695X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-695X.2011.00837.x
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femspd/article/63/1/129/549169
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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