Deciphering the effect of an oxovanadium( iv ) complex with the flavonoid chrysin (VOChrys) on intracellular cell signalling pathways in an osteosarcoma cell line

Autores
Leon, Ignacio Esteban; Diez, Paula Gabriela; Etcheverry, Susana Beatriz; Fuentes, Manuel
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vanadium complexes were studied during recent years and considered as a representative of a newclass of non-platinum metal antitumor agents in combination with their low toxicity. However, a fewchallenges still remain in the discovery of new molecular targets for these novel metal-based drugs. Thestudy of cell signaling pathways related to vanadium drugs, which is highly critical for identifying specific Q4targets that play an important role in the antitumor activity of vanadium compounds, is scarce. Thisresearch deals with the alterations in intracellular signaling pathways promoted by an oxovanadium(IV)complex with the flavonoid chrysin [VO(chrysin)2EtOH]2 (VOChrys) in a human osteosarcoma cell line(MG-63). Herein we report for the first time the effect of [VO(chrysin)2EtOH]2 on the relative abundance Q5of 224 proteins, which are involved in the most common intracellular pathways. Besides, full-lengthhuman recombinant (FAK and AKT1) kinases are produced using an in situ IVTT system and then wehave evaluated the variation of relative tyrosine-phosphorylation levels caused by the[VO(chrysin)2EtOH]2 compound. The results of the differential protein expression levels reveal thatseveral proteins such as PKB/AKT, PAK, DAPK, Cdk 4, 6 and 7, FADD, AP2, NAK, and JNK, among others,were altered. Moreover, cell signaling pathways related to the PTK2B, FAK, PKC families suggests an Q6important role associated with the antitumor activity of [VO(chrysin)2EtOH]2 was demonstrated. Finally,the effect of this compound on in situ expressed FAK and AKT1 is validated by determining thephosphorylation level, which decreased in the former and increased in the latter.
Fil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patologica; Argentina
Fil: Diez, Paula Gabriela. Universidad de Salamanca; España. Consejo Superior de Investigaciones Científicas; España
Fil: Etcheverry, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patologica; Argentina
Fil: Fuentes, Manuel. Universidad de Salamanca; España. Consejo Superior de Investigaciones Científicas; España
Materia
Cell Signalling
Osteosarcoma
Vanadium
Fak
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48422
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/48422
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Deciphering the effect of an oxovanadium( iv ) complex with the flavonoid chrysin (VOChrys) on intracellular cell signalling pathways in an osteosarcoma cell lineLeon, Ignacio EstebanDiez, Paula GabrielaEtcheverry, Susana BeatrizFuentes, ManuelCell SignallingOsteosarcomaVanadiumFakhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Vanadium complexes were studied during recent years and considered as a representative of a newclass of non-platinum metal antitumor agents in combination with their low toxicity. However, a fewchallenges still remain in the discovery of new molecular targets for these novel metal-based drugs. Thestudy of cell signaling pathways related to vanadium drugs, which is highly critical for identifying specific Q4targets that play an important role in the antitumor activity of vanadium compounds, is scarce. Thisresearch deals with the alterations in intracellular signaling pathways promoted by an oxovanadium(IV)complex with the flavonoid chrysin [VO(chrysin)2EtOH]2 (VOChrys) in a human osteosarcoma cell line(MG-63). Herein we report for the first time the effect of [VO(chrysin)2EtOH]2 on the relative abundance Q5of 224 proteins, which are involved in the most common intracellular pathways. Besides, full-lengthhuman recombinant (FAK and AKT1) kinases are produced using an in situ IVTT system and then wehave evaluated the variation of relative tyrosine-phosphorylation levels caused by the[VO(chrysin)2EtOH]2 compound. The results of the differential protein expression levels reveal thatseveral proteins such as PKB/AKT, PAK, DAPK, Cdk 4, 6 and 7, FADD, AP2, NAK, and JNK, among others,were altered. Moreover, cell signaling pathways related to the PTK2B, FAK, PKC families suggests an Q6important role associated with the antitumor activity of [VO(chrysin)2EtOH]2 was demonstrated. Finally,the effect of this compound on in situ expressed FAK and AKT1 is validated by determining thephosphorylation level, which decreased in the former and increased in the latter.Fil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patologica; ArgentinaFil: Diez, Paula Gabriela. Universidad de Salamanca; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Etcheverry, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patologica; ArgentinaFil: Fuentes, Manuel. Universidad de Salamanca; España. Consejo Superior de Investigaciones Científicas; EspañaRoyal Society of Chemistry2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48422Leon, Ignacio Esteban; Diez, Paula Gabriela; Etcheverry, Susana Beatriz; Fuentes, Manuel; Deciphering the effect of an oxovanadium( iv ) complex with the flavonoid chrysin (VOChrys) on intracellular cell signalling pathways in an osteosarcoma cell line; Royal Society of Chemistry; Metallomics; 8; 8; 5-2016; 739-7491756-5901CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1039/c6mt00045binfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2016/MT/C6MT00045Binfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:33Zoai:ri.conicet.gov.ar:11336/48422instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:33.332CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Deciphering the effect of an oxovanadium( iv ) complex with the flavonoid chrysin (VOChrys) on intracellular cell signalling pathways in an osteosarcoma cell line
title Deciphering the effect of an oxovanadium( iv ) complex with the flavonoid chrysin (VOChrys) on intracellular cell signalling pathways in an osteosarcoma cell line
spellingShingle Deciphering the effect of an oxovanadium( iv ) complex with the flavonoid chrysin (VOChrys) on intracellular cell signalling pathways in an osteosarcoma cell line
Leon, Ignacio Esteban
Cell Signalling
Osteosarcoma
Vanadium
Fak
title_short Deciphering the effect of an oxovanadium( iv ) complex with the flavonoid chrysin (VOChrys) on intracellular cell signalling pathways in an osteosarcoma cell line
title_full Deciphering the effect of an oxovanadium( iv ) complex with the flavonoid chrysin (VOChrys) on intracellular cell signalling pathways in an osteosarcoma cell line
title_fullStr Deciphering the effect of an oxovanadium( iv ) complex with the flavonoid chrysin (VOChrys) on intracellular cell signalling pathways in an osteosarcoma cell line
title_full_unstemmed Deciphering the effect of an oxovanadium( iv ) complex with the flavonoid chrysin (VOChrys) on intracellular cell signalling pathways in an osteosarcoma cell line
title_sort Deciphering the effect of an oxovanadium( iv ) complex with the flavonoid chrysin (VOChrys) on intracellular cell signalling pathways in an osteosarcoma cell line
dc.creator.none.fl_str_mv Leon, Ignacio Esteban
Diez, Paula Gabriela
Etcheverry, Susana Beatriz
Fuentes, Manuel
author Leon, Ignacio Esteban
author_facet Leon, Ignacio Esteban
Diez, Paula Gabriela
Etcheverry, Susana Beatriz
Fuentes, Manuel
author_role author
author2 Diez, Paula Gabriela
Etcheverry, Susana Beatriz
Fuentes, Manuel
author2_role author
author
author
dc.subject.none.fl_str_mv Cell Signalling
Osteosarcoma
Vanadium
Fak
topic Cell Signalling
Osteosarcoma
Vanadium
Fak
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Vanadium complexes were studied during recent years and considered as a representative of a newclass of non-platinum metal antitumor agents in combination with their low toxicity. However, a fewchallenges still remain in the discovery of new molecular targets for these novel metal-based drugs. Thestudy of cell signaling pathways related to vanadium drugs, which is highly critical for identifying specific Q4targets that play an important role in the antitumor activity of vanadium compounds, is scarce. Thisresearch deals with the alterations in intracellular signaling pathways promoted by an oxovanadium(IV)complex with the flavonoid chrysin [VO(chrysin)2EtOH]2 (VOChrys) in a human osteosarcoma cell line(MG-63). Herein we report for the first time the effect of [VO(chrysin)2EtOH]2 on the relative abundance Q5of 224 proteins, which are involved in the most common intracellular pathways. Besides, full-lengthhuman recombinant (FAK and AKT1) kinases are produced using an in situ IVTT system and then wehave evaluated the variation of relative tyrosine-phosphorylation levels caused by the[VO(chrysin)2EtOH]2 compound. The results of the differential protein expression levels reveal thatseveral proteins such as PKB/AKT, PAK, DAPK, Cdk 4, 6 and 7, FADD, AP2, NAK, and JNK, among others,were altered. Moreover, cell signaling pathways related to the PTK2B, FAK, PKC families suggests an Q6important role associated with the antitumor activity of [VO(chrysin)2EtOH]2 was demonstrated. Finally,the effect of this compound on in situ expressed FAK and AKT1 is validated by determining thephosphorylation level, which decreased in the former and increased in the latter.
Fil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patologica; Argentina
Fil: Diez, Paula Gabriela. Universidad de Salamanca; España. Consejo Superior de Investigaciones Científicas; España
Fil: Etcheverry, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patologica; Argentina
Fil: Fuentes, Manuel. Universidad de Salamanca; España. Consejo Superior de Investigaciones Científicas; España
description Vanadium complexes were studied during recent years and considered as a representative of a newclass of non-platinum metal antitumor agents in combination with their low toxicity. However, a fewchallenges still remain in the discovery of new molecular targets for these novel metal-based drugs. Thestudy of cell signaling pathways related to vanadium drugs, which is highly critical for identifying specific Q4targets that play an important role in the antitumor activity of vanadium compounds, is scarce. Thisresearch deals with the alterations in intracellular signaling pathways promoted by an oxovanadium(IV)complex with the flavonoid chrysin [VO(chrysin)2EtOH]2 (VOChrys) in a human osteosarcoma cell line(MG-63). Herein we report for the first time the effect of [VO(chrysin)2EtOH]2 on the relative abundance Q5of 224 proteins, which are involved in the most common intracellular pathways. Besides, full-lengthhuman recombinant (FAK and AKT1) kinases are produced using an in situ IVTT system and then wehave evaluated the variation of relative tyrosine-phosphorylation levels caused by the[VO(chrysin)2EtOH]2 compound. The results of the differential protein expression levels reveal thatseveral proteins such as PKB/AKT, PAK, DAPK, Cdk 4, 6 and 7, FADD, AP2, NAK, and JNK, among others,were altered. Moreover, cell signaling pathways related to the PTK2B, FAK, PKC families suggests an Q6important role associated with the antitumor activity of [VO(chrysin)2EtOH]2 was demonstrated. Finally,the effect of this compound on in situ expressed FAK and AKT1 is validated by determining thephosphorylation level, which decreased in the former and increased in the latter.
publishDate 2016
dc.date.none.fl_str_mv 2016-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48422
Leon, Ignacio Esteban; Diez, Paula Gabriela; Etcheverry, Susana Beatriz; Fuentes, Manuel; Deciphering the effect of an oxovanadium( iv ) complex with the flavonoid chrysin (VOChrys) on intracellular cell signalling pathways in an osteosarcoma cell line; Royal Society of Chemistry; Metallomics; 8; 8; 5-2016; 739-749
1756-5901
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48422
identifier_str_mv Leon, Ignacio Esteban; Diez, Paula Gabriela; Etcheverry, Susana Beatriz; Fuentes, Manuel; Deciphering the effect of an oxovanadium( iv ) complex with the flavonoid chrysin (VOChrys) on intracellular cell signalling pathways in an osteosarcoma cell line; Royal Society of Chemistry; Metallomics; 8; 8; 5-2016; 739-749
1756-5901
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1039/c6mt00045b
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2016/MT/C6MT00045B
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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