In vitro and in vivo characterization of anodised zirconium as a potential material for biomedical applications

Autores
Katunar, Maria Rosa; Gomez Sanchez, Andrea Valeria; Santos Coquillat, Ana; Civantos, Ana; Martinez Campos, Enrique; Ballarre, Josefina; Vico, Tamara Antonela; Baca, Matías; Ramos, Viviana; Cere, Silvia
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In vitro studies offer the insights for the understanding of the mechanisms at the tissue–implant interface that will provide an effective functioning in vivo. The good biocompatibility of zirconium makes a good candidate for biomedical applications and the attractive in vivo performance is mainly due to the presence of a protective oxide layer. The aim of this study is to evaluate by in vitro and in vivo approach, the influence of surface modification achieved by anodisation at 30 and 60 V on zirconium implants on the first steps of the osseointegration process. In this study cell attachment, proliferation and morphology of mouse myoblast C2C12-GFP and in mouse osteoprogenitor MC3T3-E1 cells was evaluated. Also, together with the immune system response, osteoclast differentiation and morphology with RAW 264.7 murine cell line were analysed. It was found that anodisation treatment at 60 V enhanced cell spreading and the osteoblastic and osteoclastic cells morphology, showing a strong dependence on the surface characteristics. In vivo tests were performed in a rat femur osteotomy model. Dynamical and static histological and histomorphometric analyses were developed 15 and 30 days after surgery. Newly formed bone around Zr60V implants showed a continuous newly compact and homogeneous bone just 15 after surgery, as judged by the enhanced thickness and mineralization rate. The results indicate that anodising treatment at 60 V could be an effective improvement in the osseointegration of zirconium by stimulating adhesion, proliferation, morphology, new bone thickness and bone mineral apposition, making zirconium an emerging candidate material for biomedical applications.
Fil: Katunar, Maria Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Gomez Sanchez, Andrea Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Santos Coquillat, Ana. Universidad Complutense de Madrid; España
Fil: Civantos, Ana. Instituto de Ciencia y Tecnología de Polímeros; España
Fil: Martinez Campos, Enrique. Universidad Complutense de Madrid; España
Fil: Ballarre, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Vico, Tamara Antonela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Baca, Matías. Hospital Interzonal General de Agudos “Oscar Alende”; Argentina
Fil: Ramos, Viviana. Instituto de Ciencia y Tecnología de Polímeros; España
Fil: Cere, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Materia
Biomaterials
In Vivo
Osseointegration
Zirconium Implant
Anodisation
Animal Model
Osteoclast
Osteoblast
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23243
Acceder
id CONICETDig_c73d3da3cccdfebb492ea0a6cf584491
oai_identifier_str oai:ri.conicet.gov.ar:11336/23243
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro and in vivo characterization of anodised zirconium as a potential material for biomedical applicationsKatunar, Maria RosaGomez Sanchez, Andrea ValeriaSantos Coquillat, AnaCivantos, AnaMartinez Campos, EnriqueBallarre, JosefinaVico, Tamara AntonelaBaca, MatíasRamos, VivianaCere, SilviaBiomaterialsIn VivoOsseointegrationZirconium ImplantAnodisationAnimal ModelOsteoclastOsteoblasthttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2In vitro studies offer the insights for the understanding of the mechanisms at the tissue–implant interface that will provide an effective functioning in vivo. The good biocompatibility of zirconium makes a good candidate for biomedical applications and the attractive in vivo performance is mainly due to the presence of a protective oxide layer. The aim of this study is to evaluate by in vitro and in vivo approach, the influence of surface modification achieved by anodisation at 30 and 60 V on zirconium implants on the first steps of the osseointegration process. In this study cell attachment, proliferation and morphology of mouse myoblast C2C12-GFP and in mouse osteoprogenitor MC3T3-E1 cells was evaluated. Also, together with the immune system response, osteoclast differentiation and morphology with RAW 264.7 murine cell line were analysed. It was found that anodisation treatment at 60 V enhanced cell spreading and the osteoblastic and osteoclastic cells morphology, showing a strong dependence on the surface characteristics. In vivo tests were performed in a rat femur osteotomy model. Dynamical and static histological and histomorphometric analyses were developed 15 and 30 days after surgery. Newly formed bone around Zr60V implants showed a continuous newly compact and homogeneous bone just 15 after surgery, as judged by the enhanced thickness and mineralization rate. The results indicate that anodising treatment at 60 V could be an effective improvement in the osseointegration of zirconium by stimulating adhesion, proliferation, morphology, new bone thickness and bone mineral apposition, making zirconium an emerging candidate material for biomedical applications.Fil: Katunar, Maria Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Gomez Sanchez, Andrea Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Santos Coquillat, Ana. Universidad Complutense de Madrid; EspañaFil: Civantos, Ana. Instituto de Ciencia y Tecnología de Polímeros; EspañaFil: Martinez Campos, Enrique. Universidad Complutense de Madrid; EspañaFil: Ballarre, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Vico, Tamara Antonela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Baca, Matías. Hospital Interzonal General de Agudos “Oscar Alende”; ArgentinaFil: Ramos, Viviana. Instituto de Ciencia y Tecnología de Polímeros; EspañaFil: Cere, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaElsevier Science2017-02-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23243Katunar, Maria Rosa; Gomez Sanchez, Andrea Valeria; Santos Coquillat, Ana; Civantos, Ana; Martinez Campos, Enrique; et al.; In vitro and in vivo characterization of anodised zirconium as a potential material for biomedical applications; Elsevier Science; Materials Science; 75; 27-2-2017; 957-9680928-4931CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0928493116315661info:eu-repo/semantics/altIdentifier/doi/10.1016/j.msec.2017.02.139info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:19:42Zoai:ri.conicet.gov.ar:11336/23243instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:19:43.242CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro and in vivo characterization of anodised zirconium as a potential material for biomedical applications
title In vitro and in vivo characterization of anodised zirconium as a potential material for biomedical applications
spellingShingle In vitro and in vivo characterization of anodised zirconium as a potential material for biomedical applications
Katunar, Maria Rosa
Biomaterials
In Vivo
Osseointegration
Zirconium Implant
Anodisation
Animal Model
Osteoclast
Osteoblast
title_short In vitro and in vivo characterization of anodised zirconium as a potential material for biomedical applications
title_full In vitro and in vivo characterization of anodised zirconium as a potential material for biomedical applications
title_fullStr In vitro and in vivo characterization of anodised zirconium as a potential material for biomedical applications
title_full_unstemmed In vitro and in vivo characterization of anodised zirconium as a potential material for biomedical applications
title_sort In vitro and in vivo characterization of anodised zirconium as a potential material for biomedical applications
dc.creator.none.fl_str_mv Katunar, Maria Rosa
Gomez Sanchez, Andrea Valeria
Santos Coquillat, Ana
Civantos, Ana
Martinez Campos, Enrique
Ballarre, Josefina
Vico, Tamara Antonela
Baca, Matías
Ramos, Viviana
Cere, Silvia
author Katunar, Maria Rosa
author_facet Katunar, Maria Rosa
Gomez Sanchez, Andrea Valeria
Santos Coquillat, Ana
Civantos, Ana
Martinez Campos, Enrique
Ballarre, Josefina
Vico, Tamara Antonela
Baca, Matías
Ramos, Viviana
Cere, Silvia
author_role author
author2 Gomez Sanchez, Andrea Valeria
Santos Coquillat, Ana
Civantos, Ana
Martinez Campos, Enrique
Ballarre, Josefina
Vico, Tamara Antonela
Baca, Matías
Ramos, Viviana
Cere, Silvia
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biomaterials
In Vivo
Osseointegration
Zirconium Implant
Anodisation
Animal Model
Osteoclast
Osteoblast
topic Biomaterials
In Vivo
Osseointegration
Zirconium Implant
Anodisation
Animal Model
Osteoclast
Osteoblast
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv In vitro studies offer the insights for the understanding of the mechanisms at the tissue–implant interface that will provide an effective functioning in vivo. The good biocompatibility of zirconium makes a good candidate for biomedical applications and the attractive in vivo performance is mainly due to the presence of a protective oxide layer. The aim of this study is to evaluate by in vitro and in vivo approach, the influence of surface modification achieved by anodisation at 30 and 60 V on zirconium implants on the first steps of the osseointegration process. In this study cell attachment, proliferation and morphology of mouse myoblast C2C12-GFP and in mouse osteoprogenitor MC3T3-E1 cells was evaluated. Also, together with the immune system response, osteoclast differentiation and morphology with RAW 264.7 murine cell line were analysed. It was found that anodisation treatment at 60 V enhanced cell spreading and the osteoblastic and osteoclastic cells morphology, showing a strong dependence on the surface characteristics. In vivo tests were performed in a rat femur osteotomy model. Dynamical and static histological and histomorphometric analyses were developed 15 and 30 days after surgery. Newly formed bone around Zr60V implants showed a continuous newly compact and homogeneous bone just 15 after surgery, as judged by the enhanced thickness and mineralization rate. The results indicate that anodising treatment at 60 V could be an effective improvement in the osseointegration of zirconium by stimulating adhesion, proliferation, morphology, new bone thickness and bone mineral apposition, making zirconium an emerging candidate material for biomedical applications.
Fil: Katunar, Maria Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Gomez Sanchez, Andrea Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Santos Coquillat, Ana. Universidad Complutense de Madrid; España
Fil: Civantos, Ana. Instituto de Ciencia y Tecnología de Polímeros; España
Fil: Martinez Campos, Enrique. Universidad Complutense de Madrid; España
Fil: Ballarre, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Vico, Tamara Antonela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Baca, Matías. Hospital Interzonal General de Agudos “Oscar Alende”; Argentina
Fil: Ramos, Viviana. Instituto de Ciencia y Tecnología de Polímeros; España
Fil: Cere, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
description In vitro studies offer the insights for the understanding of the mechanisms at the tissue–implant interface that will provide an effective functioning in vivo. The good biocompatibility of zirconium makes a good candidate for biomedical applications and the attractive in vivo performance is mainly due to the presence of a protective oxide layer. The aim of this study is to evaluate by in vitro and in vivo approach, the influence of surface modification achieved by anodisation at 30 and 60 V on zirconium implants on the first steps of the osseointegration process. In this study cell attachment, proliferation and morphology of mouse myoblast C2C12-GFP and in mouse osteoprogenitor MC3T3-E1 cells was evaluated. Also, together with the immune system response, osteoclast differentiation and morphology with RAW 264.7 murine cell line were analysed. It was found that anodisation treatment at 60 V enhanced cell spreading and the osteoblastic and osteoclastic cells morphology, showing a strong dependence on the surface characteristics. In vivo tests were performed in a rat femur osteotomy model. Dynamical and static histological and histomorphometric analyses were developed 15 and 30 days after surgery. Newly formed bone around Zr60V implants showed a continuous newly compact and homogeneous bone just 15 after surgery, as judged by the enhanced thickness and mineralization rate. The results indicate that anodising treatment at 60 V could be an effective improvement in the osseointegration of zirconium by stimulating adhesion, proliferation, morphology, new bone thickness and bone mineral apposition, making zirconium an emerging candidate material for biomedical applications.
publishDate 2017
dc.date.none.fl_str_mv 2017-02-27
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23243
Katunar, Maria Rosa; Gomez Sanchez, Andrea Valeria; Santos Coquillat, Ana; Civantos, Ana; Martinez Campos, Enrique; et al.; In vitro and in vivo characterization of anodised zirconium as a potential material for biomedical applications; Elsevier Science; Materials Science; 75; 27-2-2017; 957-968
0928-4931
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23243
identifier_str_mv Katunar, Maria Rosa; Gomez Sanchez, Andrea Valeria; Santos Coquillat, Ana; Civantos, Ana; Martinez Campos, Enrique; et al.; In vitro and in vivo characterization of anodised zirconium as a potential material for biomedical applications; Elsevier Science; Materials Science; 75; 27-2-2017; 957-968
0928-4931
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0928493116315661
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.msec.2017.02.139
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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