Electrochemical Regulation of Budding Yeast Polarity

Autores
Haupt, Armin; Campetelli, Alexis Nazareno; Bonazzi, Daria; Piel, Matthieu; Chang, Fred; Minc, Nicolas
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cells are naturally surrounded by organized electrical signals in the form of local ion fluxes, membrane potential, and electric fields (EFs) at their surface. Although the contribution of electrochemical elements to cell polarity and migration is beginning to be appreciated, underlying mechanisms are not known. Here we show that an exogenous EF can orient cell polarization in budding yeast (Saccharomyces cerevisiae) cells, directing the growth of mating projections towards sites of hyperpolarized membrane potential, while directing bud emergence in the opposite direction, towards sites of depolarized potential. Using an optogenetic approach, we demonstrate that a local change in membrane potential triggered by light is sufficient to direct cell polarization. Screens for mutants with altered EF responses identify genes involved in transducing electrochemical signals to the polarity machinery. Membrane potential, which is regulated by the potassium transporter Trk1p, is required for polarity orientation during mating and EF response. Membrane potential may regulate membrane charges through negatively charged phosphatidylserines (PSs), which act to position the Cdc42p-based polarity machinery. These studies thus define an electrochemical pathway that directs the orientation of cell polarization.
Fil: Haupt, Armin. Institut Jacques Monod; Francia
Fil: Campetelli, Alexis Nazareno. Institut Jacques Monod; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bonazzi, Daria. Institut Jacques Monod; Francia
Fil: Piel, Matthieu. Institute Curie; Francia
Fil: Chang, Fred. Columbia University; Estados Unidos
Fil: Minc, Nicolas. Institut Jacques Monod; Francia
Materia
ELECTROCHEMISTRY
CELL POLARITY
YEAST
S. CEREVISIAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34417
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/34417
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Electrochemical Regulation of Budding Yeast PolarityHaupt, ArminCampetelli, Alexis NazarenoBonazzi, DariaPiel, MatthieuChang, FredMinc, NicolasELECTROCHEMISTRYCELL POLARITYYEASTS. CEREVISIAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cells are naturally surrounded by organized electrical signals in the form of local ion fluxes, membrane potential, and electric fields (EFs) at their surface. Although the contribution of electrochemical elements to cell polarity and migration is beginning to be appreciated, underlying mechanisms are not known. Here we show that an exogenous EF can orient cell polarization in budding yeast (Saccharomyces cerevisiae) cells, directing the growth of mating projections towards sites of hyperpolarized membrane potential, while directing bud emergence in the opposite direction, towards sites of depolarized potential. Using an optogenetic approach, we demonstrate that a local change in membrane potential triggered by light is sufficient to direct cell polarization. Screens for mutants with altered EF responses identify genes involved in transducing electrochemical signals to the polarity machinery. Membrane potential, which is regulated by the potassium transporter Trk1p, is required for polarity orientation during mating and EF response. Membrane potential may regulate membrane charges through negatively charged phosphatidylserines (PSs), which act to position the Cdc42p-based polarity machinery. These studies thus define an electrochemical pathway that directs the orientation of cell polarization.Fil: Haupt, Armin. Institut Jacques Monod; FranciaFil: Campetelli, Alexis Nazareno. Institut Jacques Monod; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bonazzi, Daria. Institut Jacques Monod; FranciaFil: Piel, Matthieu. Institute Curie; FranciaFil: Chang, Fred. Columbia University; Estados UnidosFil: Minc, Nicolas. Institut Jacques Monod; FranciaPublic Library of Science2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34417Haupt, Armin; Campetelli, Alexis Nazareno; Bonazzi, Daria; Piel, Matthieu; Chang, Fred ; et al.; Electrochemical Regulation of Budding Yeast Polarity; Public Library of Science; Plos Biology; 12; 12; 12-2014; 1-13; e10020291544-9173CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pbio.1002029info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1002029info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:44Zoai:ri.conicet.gov.ar:11336/34417instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:44.576CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Electrochemical Regulation of Budding Yeast Polarity
title Electrochemical Regulation of Budding Yeast Polarity
spellingShingle Electrochemical Regulation of Budding Yeast Polarity
Haupt, Armin
ELECTROCHEMISTRY
CELL POLARITY
YEAST
S. CEREVISIAE
title_short Electrochemical Regulation of Budding Yeast Polarity
title_full Electrochemical Regulation of Budding Yeast Polarity
title_fullStr Electrochemical Regulation of Budding Yeast Polarity
title_full_unstemmed Electrochemical Regulation of Budding Yeast Polarity
title_sort Electrochemical Regulation of Budding Yeast Polarity
dc.creator.none.fl_str_mv Haupt, Armin
Campetelli, Alexis Nazareno
Bonazzi, Daria
Piel, Matthieu
Chang, Fred
Minc, Nicolas
author Haupt, Armin
author_facet Haupt, Armin
Campetelli, Alexis Nazareno
Bonazzi, Daria
Piel, Matthieu
Chang, Fred
Minc, Nicolas
author_role author
author2 Campetelli, Alexis Nazareno
Bonazzi, Daria
Piel, Matthieu
Chang, Fred
Minc, Nicolas
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ELECTROCHEMISTRY
CELL POLARITY
YEAST
S. CEREVISIAE
topic ELECTROCHEMISTRY
CELL POLARITY
YEAST
S. CEREVISIAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cells are naturally surrounded by organized electrical signals in the form of local ion fluxes, membrane potential, and electric fields (EFs) at their surface. Although the contribution of electrochemical elements to cell polarity and migration is beginning to be appreciated, underlying mechanisms are not known. Here we show that an exogenous EF can orient cell polarization in budding yeast (Saccharomyces cerevisiae) cells, directing the growth of mating projections towards sites of hyperpolarized membrane potential, while directing bud emergence in the opposite direction, towards sites of depolarized potential. Using an optogenetic approach, we demonstrate that a local change in membrane potential triggered by light is sufficient to direct cell polarization. Screens for mutants with altered EF responses identify genes involved in transducing electrochemical signals to the polarity machinery. Membrane potential, which is regulated by the potassium transporter Trk1p, is required for polarity orientation during mating and EF response. Membrane potential may regulate membrane charges through negatively charged phosphatidylserines (PSs), which act to position the Cdc42p-based polarity machinery. These studies thus define an electrochemical pathway that directs the orientation of cell polarization.
Fil: Haupt, Armin. Institut Jacques Monod; Francia
Fil: Campetelli, Alexis Nazareno. Institut Jacques Monod; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bonazzi, Daria. Institut Jacques Monod; Francia
Fil: Piel, Matthieu. Institute Curie; Francia
Fil: Chang, Fred. Columbia University; Estados Unidos
Fil: Minc, Nicolas. Institut Jacques Monod; Francia
description Cells are naturally surrounded by organized electrical signals in the form of local ion fluxes, membrane potential, and electric fields (EFs) at their surface. Although the contribution of electrochemical elements to cell polarity and migration is beginning to be appreciated, underlying mechanisms are not known. Here we show that an exogenous EF can orient cell polarization in budding yeast (Saccharomyces cerevisiae) cells, directing the growth of mating projections towards sites of hyperpolarized membrane potential, while directing bud emergence in the opposite direction, towards sites of depolarized potential. Using an optogenetic approach, we demonstrate that a local change in membrane potential triggered by light is sufficient to direct cell polarization. Screens for mutants with altered EF responses identify genes involved in transducing electrochemical signals to the polarity machinery. Membrane potential, which is regulated by the potassium transporter Trk1p, is required for polarity orientation during mating and EF response. Membrane potential may regulate membrane charges through negatively charged phosphatidylserines (PSs), which act to position the Cdc42p-based polarity machinery. These studies thus define an electrochemical pathway that directs the orientation of cell polarization.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34417
Haupt, Armin; Campetelli, Alexis Nazareno; Bonazzi, Daria; Piel, Matthieu; Chang, Fred ; et al.; Electrochemical Regulation of Budding Yeast Polarity; Public Library of Science; Plos Biology; 12; 12; 12-2014; 1-13; e1002029
1544-9173
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34417
identifier_str_mv Haupt, Armin; Campetelli, Alexis Nazareno; Bonazzi, Daria; Piel, Matthieu; Chang, Fred ; et al.; Electrochemical Regulation of Budding Yeast Polarity; Public Library of Science; Plos Biology; 12; 12; 12-2014; 1-13; e1002029
1544-9173
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pbio.1002029
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1002029
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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