Association of synthetic anthelmintics and natural monoterpenes against Haemonchus contortus

Autores
Costa Junior, Livio; Silva, Carolina R.; Macedo, Sara R. D.; Campos, Nagilla R. C. L.; Lifschitz, Adrian Luis
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The resistance of Haemonchus contortus to synthetic anthelmintics is an increasing concern and different strategies are being evaluated. The present trial studied the in vitro effect of the association of synthetic compound and natural monoterpene on eggs and larvae of H. contortus. The monoterpenes carvacrol, thymol, r-carvone, s-carvone, citral and p-cymene, and the synthetic antihelmintic ivermectin, and albendazole were used. Egg Hatch Test (EHT) and Larval Migration Inhibition Test (LMIT) were performed. The lowest efficient concentration of monoterpenes in EHT (≤ 11% of efficacy) and LMIT (≤ 18% of efficacy) was used in association with different concentration of synthetic compound. The IC50 and Synergism Rate (SR) were calculated. The highest efficiency of monoterpenes in EHT was obtained with r-carvone (IC50 = 0.25 mg/mL) and s-carvone (IC50 = 0.79 mg/mL) and in the LMIT with r-carvone (IC50 = 0.60 mg/mL). The best association was observed in the EHT with albendazol (thymol SR: 2.9 and r-carvone SR: 1.6) and ivermectin (citral SR: 1.9 and carvacrol SR:1.7). No synergistic effect was obtaining using the LMIT. The combination of synthetic compound and natural monoterpenes could be positive to gastrointestinal nematodes control: However this strategy should be carefully analysed due to the possibility of antagonic effects among the different compounds.
Fil: Costa Junior, Livio. Universidade Federal do Maranhao; Brasil
Fil: Silva, Carolina R.. Universidade Federal do Maranhao; Brasil
Fil: Macedo, Sara R. D.. Universidade Federal do Maranhao; Brasil
Fil: Campos, Nagilla R. C. L.. Universidade Federal do Maranhao; Brasil
Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
26th Conference World Association for the Advancement of Veterinary Parasitology
Kuala Lumpur
Malasia
World Association for the Advancement of Veterinary Parasitology
Materia
Carvacrol
Citral
Thymol
Nematode
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/176259
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/176259
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Association of synthetic anthelmintics and natural monoterpenes against Haemonchus contortusCosta Junior, LivioSilva, Carolina R.Macedo, Sara R. D.Campos, Nagilla R. C. L.Lifschitz, Adrian LuisCarvacrolCitralThymolNematodehttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The resistance of Haemonchus contortus to synthetic anthelmintics is an increasing concern and different strategies are being evaluated. The present trial studied the in vitro effect of the association of synthetic compound and natural monoterpene on eggs and larvae of H. contortus. The monoterpenes carvacrol, thymol, r-carvone, s-carvone, citral and p-cymene, and the synthetic antihelmintic ivermectin, and albendazole were used. Egg Hatch Test (EHT) and Larval Migration Inhibition Test (LMIT) were performed. The lowest efficient concentration of monoterpenes in EHT (≤ 11% of efficacy) and LMIT (≤ 18% of efficacy) was used in association with different concentration of synthetic compound. The IC50 and Synergism Rate (SR) were calculated. The highest efficiency of monoterpenes in EHT was obtained with r-carvone (IC50 = 0.25 mg/mL) and s-carvone (IC50 = 0.79 mg/mL) and in the LMIT with r-carvone (IC50 = 0.60 mg/mL). The best association was observed in the EHT with albendazol (thymol SR: 2.9 and r-carvone SR: 1.6) and ivermectin (citral SR: 1.9 and carvacrol SR:1.7). No synergistic effect was obtaining using the LMIT. The combination of synthetic compound and natural monoterpenes could be positive to gastrointestinal nematodes control: However this strategy should be carefully analysed due to the possibility of antagonic effects among the different compounds.Fil: Costa Junior, Livio. Universidade Federal do Maranhao; BrasilFil: Silva, Carolina R.. Universidade Federal do Maranhao; BrasilFil: Macedo, Sara R. D.. Universidade Federal do Maranhao; BrasilFil: Campos, Nagilla R. C. L.. Universidade Federal do Maranhao; BrasilFil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina26th Conference World Association for the Advancement of Veterinary ParasitologyKuala LumpurMalasiaWorld Association for the Advancement of Veterinary ParasitologyWorld Association for the Advancement of Veterinary Parasitology2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/176259Association of synthetic anthelmintics and natural monoterpenes against Haemonchus contortus; 26th Conference World Association for the Advancement of Veterinary Parasitology ; Kuala Lumpur; Malasia; 2017; 440-440CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://web.archive.org/web/20170605002243/http://www.waavp2017kl.org/info:eu-repo/semantics/altIdentifier/url/https://ir.unimas.my/id/eprint/18437/1/WAAVP%202017%20Abstract%20Book.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:12:23Zoai:ri.conicet.gov.ar:11336/176259instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:12:23.929CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Association of synthetic anthelmintics and natural monoterpenes against Haemonchus contortus
title Association of synthetic anthelmintics and natural monoterpenes against Haemonchus contortus
spellingShingle Association of synthetic anthelmintics and natural monoterpenes against Haemonchus contortus
Costa Junior, Livio
Carvacrol
Citral
Thymol
Nematode
title_short Association of synthetic anthelmintics and natural monoterpenes against Haemonchus contortus
title_full Association of synthetic anthelmintics and natural monoterpenes against Haemonchus contortus
title_fullStr Association of synthetic anthelmintics and natural monoterpenes against Haemonchus contortus
title_full_unstemmed Association of synthetic anthelmintics and natural monoterpenes against Haemonchus contortus
title_sort Association of synthetic anthelmintics and natural monoterpenes against Haemonchus contortus
dc.creator.none.fl_str_mv Costa Junior, Livio
Silva, Carolina R.
Macedo, Sara R. D.
Campos, Nagilla R. C. L.
Lifschitz, Adrian Luis
author Costa Junior, Livio
author_facet Costa Junior, Livio
Silva, Carolina R.
Macedo, Sara R. D.
Campos, Nagilla R. C. L.
Lifschitz, Adrian Luis
author_role author
author2 Silva, Carolina R.
Macedo, Sara R. D.
Campos, Nagilla R. C. L.
Lifschitz, Adrian Luis
author2_role author
author
author
author
dc.subject.none.fl_str_mv Carvacrol
Citral
Thymol
Nematode
topic Carvacrol
Citral
Thymol
Nematode
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The resistance of Haemonchus contortus to synthetic anthelmintics is an increasing concern and different strategies are being evaluated. The present trial studied the in vitro effect of the association of synthetic compound and natural monoterpene on eggs and larvae of H. contortus. The monoterpenes carvacrol, thymol, r-carvone, s-carvone, citral and p-cymene, and the synthetic antihelmintic ivermectin, and albendazole were used. Egg Hatch Test (EHT) and Larval Migration Inhibition Test (LMIT) were performed. The lowest efficient concentration of monoterpenes in EHT (≤ 11% of efficacy) and LMIT (≤ 18% of efficacy) was used in association with different concentration of synthetic compound. The IC50 and Synergism Rate (SR) were calculated. The highest efficiency of monoterpenes in EHT was obtained with r-carvone (IC50 = 0.25 mg/mL) and s-carvone (IC50 = 0.79 mg/mL) and in the LMIT with r-carvone (IC50 = 0.60 mg/mL). The best association was observed in the EHT with albendazol (thymol SR: 2.9 and r-carvone SR: 1.6) and ivermectin (citral SR: 1.9 and carvacrol SR:1.7). No synergistic effect was obtaining using the LMIT. The combination of synthetic compound and natural monoterpenes could be positive to gastrointestinal nematodes control: However this strategy should be carefully analysed due to the possibility of antagonic effects among the different compounds.
Fil: Costa Junior, Livio. Universidade Federal do Maranhao; Brasil
Fil: Silva, Carolina R.. Universidade Federal do Maranhao; Brasil
Fil: Macedo, Sara R. D.. Universidade Federal do Maranhao; Brasil
Fil: Campos, Nagilla R. C. L.. Universidade Federal do Maranhao; Brasil
Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
26th Conference World Association for the Advancement of Veterinary Parasitology
Kuala Lumpur
Malasia
World Association for the Advancement of Veterinary Parasitology
description The resistance of Haemonchus contortus to synthetic anthelmintics is an increasing concern and different strategies are being evaluated. The present trial studied the in vitro effect of the association of synthetic compound and natural monoterpene on eggs and larvae of H. contortus. The monoterpenes carvacrol, thymol, r-carvone, s-carvone, citral and p-cymene, and the synthetic antihelmintic ivermectin, and albendazole were used. Egg Hatch Test (EHT) and Larval Migration Inhibition Test (LMIT) were performed. The lowest efficient concentration of monoterpenes in EHT (≤ 11% of efficacy) and LMIT (≤ 18% of efficacy) was used in association with different concentration of synthetic compound. The IC50 and Synergism Rate (SR) were calculated. The highest efficiency of monoterpenes in EHT was obtained with r-carvone (IC50 = 0.25 mg/mL) and s-carvone (IC50 = 0.79 mg/mL) and in the LMIT with r-carvone (IC50 = 0.60 mg/mL). The best association was observed in the EHT with albendazol (thymol SR: 2.9 and r-carvone SR: 1.6) and ivermectin (citral SR: 1.9 and carvacrol SR:1.7). No synergistic effect was obtaining using the LMIT. The combination of synthetic compound and natural monoterpenes could be positive to gastrointestinal nematodes control: However this strategy should be carefully analysed due to the possibility of antagonic effects among the different compounds.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/176259
Association of synthetic anthelmintics and natural monoterpenes against Haemonchus contortus; 26th Conference World Association for the Advancement of Veterinary Parasitology ; Kuala Lumpur; Malasia; 2017; 440-440
CONICET Digital
CONICET
url http://hdl.handle.net/11336/176259
identifier_str_mv Association of synthetic anthelmintics and natural monoterpenes against Haemonchus contortus; 26th Conference World Association for the Advancement of Veterinary Parasitology ; Kuala Lumpur; Malasia; 2017; 440-440
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://web.archive.org/web/20170605002243/http://www.waavp2017kl.org/
info:eu-repo/semantics/altIdentifier/url/https://ir.unimas.my/id/eprint/18437/1/WAAVP%202017%20Abstract%20Book.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv World Association for the Advancement of Veterinary Parasitology
publisher.none.fl_str_mv World Association for the Advancement of Veterinary Parasitology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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