Combination of bioactive phytochemical and synthetic anthelmintics: in vivo and in vitro assessment of the albendazole-thymol association

Autores
Miró, María Victoria; Luque, Sonia Elisabet; Lloberas, Maria Mercedes; Costa Junior, Livio; Lanusse, Carlos Edmundo; Virkel, Guillermo Leon; Lifschitz, Adrian Luis
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The search of novel strategies is an urgent need considering the widespread increasing of anthelmintic resistance in livestock. Bioactive phytochemicals may contribute to improve parasite control by enhancing the effect of existing anthelmintic drugs. This work evaluated the in-vivo and in-vitro pharmaco-chemical interaction and the in-vivo efficacy of the combination of albendazole (ABZ) with a phenolic natural monoterpene, thymol (TML), in lambs naturally infected with resistant gastrointestinal nematodes. Thirty (30) lambs were allocated into three (3) experimental groups. Each group was treated orally with either ABZ (5 mg/kg), Thymol (150 mg/kg, twice every 24 h) or the co-administration of both compounds. Blood samples were collected between 0 and 51 h post-treatment and TML, ABZ and its metabolites were determined by HPLC. Individual faecal samples were collected at days -1 and 14 post-treatment to perform the faecal eggs count reduction test. Additionally, the effect of TML on the metabolic sulphoreduction and the sulphonation of ABZ sulphoxide was in-vitro assessed using ruminal content and liver microsomes, respectively. No changes on the pharmacokinetic behavior of ABZ sulphoxide were observed in the presence of the natural product (TML). In contrast, the ABZ sulphone Cmax and AUC were lower (p<0.05) in the co-administered animals (0.16±0.07 µg/mL y 3.63±1.21 µg.h/mL) compared with those that received ABZ alone (0.45±0.15 µg/mL and 9.50±2.84 µg.h/mL). TML was detected in the bloodstream between 1 and 51 h post-treatment, which indicates the time of target nematodes exposure to the bioactive monoterpene. However, the in-vivo efficacy of TML was 0% and the presence of TML did not increase the efficacy of ABZ. The presence of TML inhibited significantly (P< 0.05) the ruminal sulphoreduction and the hepatic sulphonation of ABZ sulphoxide. In-vivo pharmaco-parasitological studies are relevant to corroborate the adverse kinetic/metabolic interactions and the efficacy of bioactive natural products combined with synthetic anthelmintics.
Fil: Miró, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Luque, Sonia Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lloberas, Maria Mercedes. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Área de Investigación en Producción y Sanidad Animal; Argentina
Fil: Costa Junior, Livio. Universidade Federal Do Maranhao.; Brasil
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Virkel, Guillermo Leon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
27th Conference of the World Association for the Advancement of Veterinary Parasitology
Madison
Estados Unidos
World Association for the Advancement of Veterinary Parasitology
Materia
Bioactive Phytochemicals
Albendazole
Thymol
Sheep
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/160738
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Combination of bioactive phytochemical and synthetic anthelmintics: in vivo and in vitro assessment of the albendazole-thymol associationMiró, María VictoriaLuque, Sonia ElisabetLloberas, Maria MercedesCosta Junior, LivioLanusse, Carlos EdmundoVirkel, Guillermo LeonLifschitz, Adrian LuisBioactive PhytochemicalsAlbendazoleThymolSheephttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The search of novel strategies is an urgent need considering the widespread increasing of anthelmintic resistance in livestock. Bioactive phytochemicals may contribute to improve parasite control by enhancing the effect of existing anthelmintic drugs. This work evaluated the in-vivo and in-vitro pharmaco-chemical interaction and the in-vivo efficacy of the combination of albendazole (ABZ) with a phenolic natural monoterpene, thymol (TML), in lambs naturally infected with resistant gastrointestinal nematodes. Thirty (30) lambs were allocated into three (3) experimental groups. Each group was treated orally with either ABZ (5 mg/kg), Thymol (150 mg/kg, twice every 24 h) or the co-administration of both compounds. Blood samples were collected between 0 and 51 h post-treatment and TML, ABZ and its metabolites were determined by HPLC. Individual faecal samples were collected at days -1 and 14 post-treatment to perform the faecal eggs count reduction test. Additionally, the effect of TML on the metabolic sulphoreduction and the sulphonation of ABZ sulphoxide was in-vitro assessed using ruminal content and liver microsomes, respectively. No changes on the pharmacokinetic behavior of ABZ sulphoxide were observed in the presence of the natural product (TML). In contrast, the ABZ sulphone Cmax and AUC were lower (p<0.05) in the co-administered animals (0.16±0.07 µg/mL y 3.63±1.21 µg.h/mL) compared with those that received ABZ alone (0.45±0.15 µg/mL and 9.50±2.84 µg.h/mL). TML was detected in the bloodstream between 1 and 51 h post-treatment, which indicates the time of target nematodes exposure to the bioactive monoterpene. However, the in-vivo efficacy of TML was 0% and the presence of TML did not increase the efficacy of ABZ. The presence of TML inhibited significantly (P< 0.05) the ruminal sulphoreduction and the hepatic sulphonation of ABZ sulphoxide. In-vivo pharmaco-parasitological studies are relevant to corroborate the adverse kinetic/metabolic interactions and the efficacy of bioactive natural products combined with synthetic anthelmintics.Fil: Miró, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Luque, Sonia Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Lloberas, Maria Mercedes. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Área de Investigación en Producción y Sanidad Animal; ArgentinaFil: Costa Junior, Livio. Universidade Federal Do Maranhao.; BrasilFil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Virkel, Guillermo Leon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina27th Conference of the World Association for the Advancement of Veterinary ParasitologyMadisonEstados UnidosWorld Association for the Advancement of Veterinary ParasitologyWorld Association for the Advancement of Veterinary Parasitology2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/160738Combination of bioactive phytochemical and synthetic anthelmintics: in vivo and in vitro assessment of the albendazole-thymol association; 27th Conference of the World Association for the Advancement of Veterinary Parasitology; Madison; Estados Unidos; 2019; 278CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.waavp2019.com/wp-content/uploads/2019/07/WAAVP2019-Abstract-Book.pdfinfo:eu-repo/semantics/altIdentifier/url/http://www.waavp2019.comInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:25Zoai:ri.conicet.gov.ar:11336/160738instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:25.631CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Combination of bioactive phytochemical and synthetic anthelmintics: in vivo and in vitro assessment of the albendazole-thymol association
title Combination of bioactive phytochemical and synthetic anthelmintics: in vivo and in vitro assessment of the albendazole-thymol association
spellingShingle Combination of bioactive phytochemical and synthetic anthelmintics: in vivo and in vitro assessment of the albendazole-thymol association
Miró, María Victoria
Bioactive Phytochemicals
Albendazole
Thymol
Sheep
title_short Combination of bioactive phytochemical and synthetic anthelmintics: in vivo and in vitro assessment of the albendazole-thymol association
title_full Combination of bioactive phytochemical and synthetic anthelmintics: in vivo and in vitro assessment of the albendazole-thymol association
title_fullStr Combination of bioactive phytochemical and synthetic anthelmintics: in vivo and in vitro assessment of the albendazole-thymol association
title_full_unstemmed Combination of bioactive phytochemical and synthetic anthelmintics: in vivo and in vitro assessment of the albendazole-thymol association
title_sort Combination of bioactive phytochemical and synthetic anthelmintics: in vivo and in vitro assessment of the albendazole-thymol association
dc.creator.none.fl_str_mv Miró, María Victoria
Luque, Sonia Elisabet
Lloberas, Maria Mercedes
Costa Junior, Livio
Lanusse, Carlos Edmundo
Virkel, Guillermo Leon
Lifschitz, Adrian Luis
author Miró, María Victoria
author_facet Miró, María Victoria
Luque, Sonia Elisabet
Lloberas, Maria Mercedes
Costa Junior, Livio
Lanusse, Carlos Edmundo
Virkel, Guillermo Leon
Lifschitz, Adrian Luis
author_role author
author2 Luque, Sonia Elisabet
Lloberas, Maria Mercedes
Costa Junior, Livio
Lanusse, Carlos Edmundo
Virkel, Guillermo Leon
Lifschitz, Adrian Luis
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Bioactive Phytochemicals
Albendazole
Thymol
Sheep
topic Bioactive Phytochemicals
Albendazole
Thymol
Sheep
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The search of novel strategies is an urgent need considering the widespread increasing of anthelmintic resistance in livestock. Bioactive phytochemicals may contribute to improve parasite control by enhancing the effect of existing anthelmintic drugs. This work evaluated the in-vivo and in-vitro pharmaco-chemical interaction and the in-vivo efficacy of the combination of albendazole (ABZ) with a phenolic natural monoterpene, thymol (TML), in lambs naturally infected with resistant gastrointestinal nematodes. Thirty (30) lambs were allocated into three (3) experimental groups. Each group was treated orally with either ABZ (5 mg/kg), Thymol (150 mg/kg, twice every 24 h) or the co-administration of both compounds. Blood samples were collected between 0 and 51 h post-treatment and TML, ABZ and its metabolites were determined by HPLC. Individual faecal samples were collected at days -1 and 14 post-treatment to perform the faecal eggs count reduction test. Additionally, the effect of TML on the metabolic sulphoreduction and the sulphonation of ABZ sulphoxide was in-vitro assessed using ruminal content and liver microsomes, respectively. No changes on the pharmacokinetic behavior of ABZ sulphoxide were observed in the presence of the natural product (TML). In contrast, the ABZ sulphone Cmax and AUC were lower (p<0.05) in the co-administered animals (0.16±0.07 µg/mL y 3.63±1.21 µg.h/mL) compared with those that received ABZ alone (0.45±0.15 µg/mL and 9.50±2.84 µg.h/mL). TML was detected in the bloodstream between 1 and 51 h post-treatment, which indicates the time of target nematodes exposure to the bioactive monoterpene. However, the in-vivo efficacy of TML was 0% and the presence of TML did not increase the efficacy of ABZ. The presence of TML inhibited significantly (P< 0.05) the ruminal sulphoreduction and the hepatic sulphonation of ABZ sulphoxide. In-vivo pharmaco-parasitological studies are relevant to corroborate the adverse kinetic/metabolic interactions and the efficacy of bioactive natural products combined with synthetic anthelmintics.
Fil: Miró, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Luque, Sonia Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lloberas, Maria Mercedes. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Área de Investigación en Producción y Sanidad Animal; Argentina
Fil: Costa Junior, Livio. Universidade Federal Do Maranhao.; Brasil
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Virkel, Guillermo Leon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lifschitz, Adrian Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
27th Conference of the World Association for the Advancement of Veterinary Parasitology
Madison
Estados Unidos
World Association for the Advancement of Veterinary Parasitology
description The search of novel strategies is an urgent need considering the widespread increasing of anthelmintic resistance in livestock. Bioactive phytochemicals may contribute to improve parasite control by enhancing the effect of existing anthelmintic drugs. This work evaluated the in-vivo and in-vitro pharmaco-chemical interaction and the in-vivo efficacy of the combination of albendazole (ABZ) with a phenolic natural monoterpene, thymol (TML), in lambs naturally infected with resistant gastrointestinal nematodes. Thirty (30) lambs were allocated into three (3) experimental groups. Each group was treated orally with either ABZ (5 mg/kg), Thymol (150 mg/kg, twice every 24 h) or the co-administration of both compounds. Blood samples were collected between 0 and 51 h post-treatment and TML, ABZ and its metabolites were determined by HPLC. Individual faecal samples were collected at days -1 and 14 post-treatment to perform the faecal eggs count reduction test. Additionally, the effect of TML on the metabolic sulphoreduction and the sulphonation of ABZ sulphoxide was in-vitro assessed using ruminal content and liver microsomes, respectively. No changes on the pharmacokinetic behavior of ABZ sulphoxide were observed in the presence of the natural product (TML). In contrast, the ABZ sulphone Cmax and AUC were lower (p<0.05) in the co-administered animals (0.16±0.07 µg/mL y 3.63±1.21 µg.h/mL) compared with those that received ABZ alone (0.45±0.15 µg/mL and 9.50±2.84 µg.h/mL). TML was detected in the bloodstream between 1 and 51 h post-treatment, which indicates the time of target nematodes exposure to the bioactive monoterpene. However, the in-vivo efficacy of TML was 0% and the presence of TML did not increase the efficacy of ABZ. The presence of TML inhibited significantly (P< 0.05) the ruminal sulphoreduction and the hepatic sulphonation of ABZ sulphoxide. In-vivo pharmaco-parasitological studies are relevant to corroborate the adverse kinetic/metabolic interactions and the efficacy of bioactive natural products combined with synthetic anthelmintics.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/160738
Combination of bioactive phytochemical and synthetic anthelmintics: in vivo and in vitro assessment of the albendazole-thymol association; 27th Conference of the World Association for the Advancement of Veterinary Parasitology; Madison; Estados Unidos; 2019; 278
CONICET Digital
CONICET
url http://hdl.handle.net/11336/160738
identifier_str_mv Combination of bioactive phytochemical and synthetic anthelmintics: in vivo and in vitro assessment of the albendazole-thymol association; 27th Conference of the World Association for the Advancement of Veterinary Parasitology; Madison; Estados Unidos; 2019; 278
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv World Association for the Advancement of Veterinary Parasitology
publisher.none.fl_str_mv World Association for the Advancement of Veterinary Parasitology
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