Untargeted metabolomic LC-MS fingerprinting of apple cultivars for the identification of biomarkers related to resistance to rosy apple aphid

Autores
Sasía Arriba, Andrea; Alonso Salces, Rosa Maria; Asensio Regalado, Carlos; Poliero, Aimará Ayelen; Abad García, Beatriz; Dapena, Enrique; Gallo, Blanca; Berrueta, Luis Ángel
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Phenolic compounds may play important roles in plant responses to biotic-stress and in resistance mechanisms. In a previous work, we demonstrated the correlation between phenolic composition of apple cultivars and their tolerance to rosy apple aphid (RAA), Dysaphis plantaginea, a key pest of apple. The study was performed on a full-sib family produced at SERIDA, derived from a crossing between the local apple cultivar ?Meana? and the resistant cultivar ?Florina?. It consisted in the determination of the concentrations of individual flavan 3 ols, hydroxycinnamic acids, dihydrochalcones and flavonols by UHPLC-DAD present in the two parents and 154 descendants; and their resistance to RAA was determined after aphid inoculation in greenhouse. A relationship between the concentration of three caffeoylquinic acid isomers (4-CQA, 1-CQA and 3-CQA) and 4-p-coumaroylquinic acid (4-pCoQA) and the resistance to RAA was found by pattern recognition techniques. Cultivar resistance to RAA was concluded to be related with the phenylpropanoid pathway; the isomerisations being the key metabolic reactions for a cultivar to be resistant or susceptible to RAA.In the present work, the phenolic profiles of the same set of samples were analysed by UHPLC-ESI(+)-QTOF/MS to use an untargeted metabolomic approach to identify biomarkers related to resistance to RAA. Apple phenolic fingerprints and their resistance to RAA were submitted to multivariate data analysis by Principal Component Analysis, Hierarchical Clustering Analysis, Partial Least Square-Discriminant Analysis (PLSDA) and Orthogonal-PLSDA (O-PLSDA), which disclosed that the main features related to resistant cultivars were due to 4-CQA, and those related to susceptible cultivars to 5-pCoQA. These results agreed with previous ones since both phenolics are involved in the isomerisation reactions mentioned above.
Fil: Sasía Arriba, Andrea. Universidad del País Vasco; España
Fil: Alonso Salces, Rosa Maria. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina
Fil: Asensio Regalado, Carlos. Universidad del País Vasco; España
Fil: Poliero, Aimará Ayelen. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina
Fil: Abad García, Beatriz. Universidad del País Vasco; España
Fil: Dapena, Enrique. No especifíca;
Fil: Gallo, Blanca. Universidad del País Vasco; España
Fil: Berrueta, Luis Ángel. Universidad del País Vasco; España
XXX International Conference on Polyphenols
Turku
Finlandia
International Association of Groupe Polyphenols
University of Turku
Materia
MALUS DOMESTICA
DYSAPHIS PLANTAGINEA
PLANT RESISTANCE
UHPLC-QTOF/MS
CHEMOMETRICS
HYDROXYCINNAMIC ACID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/173966
Acceder
id CONICETDig_c70805dd9cca40ce352f38bbe72cd4f4
oai_identifier_str oai:ri.conicet.gov.ar:11336/173966
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Untargeted metabolomic LC-MS fingerprinting of apple cultivars for the identification of biomarkers related to resistance to rosy apple aphidSasía Arriba, AndreaAlonso Salces, Rosa MariaAsensio Regalado, CarlosPoliero, Aimará AyelenAbad García, BeatrizDapena, EnriqueGallo, BlancaBerrueta, Luis ÁngelMALUS DOMESTICADYSAPHIS PLANTAGINEAPLANT RESISTANCEUHPLC-QTOF/MSCHEMOMETRICSHYDROXYCINNAMIC ACIDhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Phenolic compounds may play important roles in plant responses to biotic-stress and in resistance mechanisms. In a previous work, we demonstrated the correlation between phenolic composition of apple cultivars and their tolerance to rosy apple aphid (RAA), Dysaphis plantaginea, a key pest of apple. The study was performed on a full-sib family produced at SERIDA, derived from a crossing between the local apple cultivar ?Meana? and the resistant cultivar ?Florina?. It consisted in the determination of the concentrations of individual flavan 3 ols, hydroxycinnamic acids, dihydrochalcones and flavonols by UHPLC-DAD present in the two parents and 154 descendants; and their resistance to RAA was determined after aphid inoculation in greenhouse. A relationship between the concentration of three caffeoylquinic acid isomers (4-CQA, 1-CQA and 3-CQA) and 4-p-coumaroylquinic acid (4-pCoQA) and the resistance to RAA was found by pattern recognition techniques. Cultivar resistance to RAA was concluded to be related with the phenylpropanoid pathway; the isomerisations being the key metabolic reactions for a cultivar to be resistant or susceptible to RAA.In the present work, the phenolic profiles of the same set of samples were analysed by UHPLC-ESI(+)-QTOF/MS to use an untargeted metabolomic approach to identify biomarkers related to resistance to RAA. Apple phenolic fingerprints and their resistance to RAA were submitted to multivariate data analysis by Principal Component Analysis, Hierarchical Clustering Analysis, Partial Least Square-Discriminant Analysis (PLSDA) and Orthogonal-PLSDA (O-PLSDA), which disclosed that the main features related to resistant cultivars were due to 4-CQA, and those related to susceptible cultivars to 5-pCoQA. These results agreed with previous ones since both phenolics are involved in the isomerisation reactions mentioned above.Fil: Sasía Arriba, Andrea. Universidad del País Vasco; EspañaFil: Alonso Salces, Rosa Maria. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Asensio Regalado, Carlos. Universidad del País Vasco; EspañaFil: Poliero, Aimará Ayelen. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Abad García, Beatriz. Universidad del País Vasco; EspañaFil: Dapena, Enrique. No especifíca;Fil: Gallo, Blanca. Universidad del País Vasco; EspañaFil: Berrueta, Luis Ángel. Universidad del País Vasco; EspañaXXX International Conference on PolyphenolsTurkuFinlandiaInternational Association of Groupe PolyphenolsUniversity of TurkuInternational Association of Groupe Polyphenols2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/173966Untargeted metabolomic LC-MS fingerprinting of apple cultivars for the identification of biomarkers related to resistance to rosy apple aphid; XXX International Conference on Polyphenols; Turku; Finlandia; 2021; 283-2842737-064X2957-4056CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.groupepolyphenols.com/wp-content/uploads/2022/06/Polyphenols-Communications-e-Vol-1.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:05:44Zoai:ri.conicet.gov.ar:11336/173966instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:05:45.038CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Untargeted metabolomic LC-MS fingerprinting of apple cultivars for the identification of biomarkers related to resistance to rosy apple aphid
title Untargeted metabolomic LC-MS fingerprinting of apple cultivars for the identification of biomarkers related to resistance to rosy apple aphid
spellingShingle Untargeted metabolomic LC-MS fingerprinting of apple cultivars for the identification of biomarkers related to resistance to rosy apple aphid
Sasía Arriba, Andrea
MALUS DOMESTICA
DYSAPHIS PLANTAGINEA
PLANT RESISTANCE
UHPLC-QTOF/MS
CHEMOMETRICS
HYDROXYCINNAMIC ACID
title_short Untargeted metabolomic LC-MS fingerprinting of apple cultivars for the identification of biomarkers related to resistance to rosy apple aphid
title_full Untargeted metabolomic LC-MS fingerprinting of apple cultivars for the identification of biomarkers related to resistance to rosy apple aphid
title_fullStr Untargeted metabolomic LC-MS fingerprinting of apple cultivars for the identification of biomarkers related to resistance to rosy apple aphid
title_full_unstemmed Untargeted metabolomic LC-MS fingerprinting of apple cultivars for the identification of biomarkers related to resistance to rosy apple aphid
title_sort Untargeted metabolomic LC-MS fingerprinting of apple cultivars for the identification of biomarkers related to resistance to rosy apple aphid
dc.creator.none.fl_str_mv Sasía Arriba, Andrea
Alonso Salces, Rosa Maria
Asensio Regalado, Carlos
Poliero, Aimará Ayelen
Abad García, Beatriz
Dapena, Enrique
Gallo, Blanca
Berrueta, Luis Ángel
author Sasía Arriba, Andrea
author_facet Sasía Arriba, Andrea
Alonso Salces, Rosa Maria
Asensio Regalado, Carlos
Poliero, Aimará Ayelen
Abad García, Beatriz
Dapena, Enrique
Gallo, Blanca
Berrueta, Luis Ángel
author_role author
author2 Alonso Salces, Rosa Maria
Asensio Regalado, Carlos
Poliero, Aimará Ayelen
Abad García, Beatriz
Dapena, Enrique
Gallo, Blanca
Berrueta, Luis Ángel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MALUS DOMESTICA
DYSAPHIS PLANTAGINEA
PLANT RESISTANCE
UHPLC-QTOF/MS
CHEMOMETRICS
HYDROXYCINNAMIC ACID
topic MALUS DOMESTICA
DYSAPHIS PLANTAGINEA
PLANT RESISTANCE
UHPLC-QTOF/MS
CHEMOMETRICS
HYDROXYCINNAMIC ACID
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Phenolic compounds may play important roles in plant responses to biotic-stress and in resistance mechanisms. In a previous work, we demonstrated the correlation between phenolic composition of apple cultivars and their tolerance to rosy apple aphid (RAA), Dysaphis plantaginea, a key pest of apple. The study was performed on a full-sib family produced at SERIDA, derived from a crossing between the local apple cultivar ?Meana? and the resistant cultivar ?Florina?. It consisted in the determination of the concentrations of individual flavan 3 ols, hydroxycinnamic acids, dihydrochalcones and flavonols by UHPLC-DAD present in the two parents and 154 descendants; and their resistance to RAA was determined after aphid inoculation in greenhouse. A relationship between the concentration of three caffeoylquinic acid isomers (4-CQA, 1-CQA and 3-CQA) and 4-p-coumaroylquinic acid (4-pCoQA) and the resistance to RAA was found by pattern recognition techniques. Cultivar resistance to RAA was concluded to be related with the phenylpropanoid pathway; the isomerisations being the key metabolic reactions for a cultivar to be resistant or susceptible to RAA.In the present work, the phenolic profiles of the same set of samples were analysed by UHPLC-ESI(+)-QTOF/MS to use an untargeted metabolomic approach to identify biomarkers related to resistance to RAA. Apple phenolic fingerprints and their resistance to RAA were submitted to multivariate data analysis by Principal Component Analysis, Hierarchical Clustering Analysis, Partial Least Square-Discriminant Analysis (PLSDA) and Orthogonal-PLSDA (O-PLSDA), which disclosed that the main features related to resistant cultivars were due to 4-CQA, and those related to susceptible cultivars to 5-pCoQA. These results agreed with previous ones since both phenolics are involved in the isomerisation reactions mentioned above.
Fil: Sasía Arriba, Andrea. Universidad del País Vasco; España
Fil: Alonso Salces, Rosa Maria. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina
Fil: Asensio Regalado, Carlos. Universidad del País Vasco; España
Fil: Poliero, Aimará Ayelen. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina
Fil: Abad García, Beatriz. Universidad del País Vasco; España
Fil: Dapena, Enrique. No especifíca;
Fil: Gallo, Blanca. Universidad del País Vasco; España
Fil: Berrueta, Luis Ángel. Universidad del País Vasco; España
XXX International Conference on Polyphenols
Turku
Finlandia
International Association of Groupe Polyphenols
University of Turku
description Phenolic compounds may play important roles in plant responses to biotic-stress and in resistance mechanisms. In a previous work, we demonstrated the correlation between phenolic composition of apple cultivars and their tolerance to rosy apple aphid (RAA), Dysaphis plantaginea, a key pest of apple. The study was performed on a full-sib family produced at SERIDA, derived from a crossing between the local apple cultivar ?Meana? and the resistant cultivar ?Florina?. It consisted in the determination of the concentrations of individual flavan 3 ols, hydroxycinnamic acids, dihydrochalcones and flavonols by UHPLC-DAD present in the two parents and 154 descendants; and their resistance to RAA was determined after aphid inoculation in greenhouse. A relationship between the concentration of three caffeoylquinic acid isomers (4-CQA, 1-CQA and 3-CQA) and 4-p-coumaroylquinic acid (4-pCoQA) and the resistance to RAA was found by pattern recognition techniques. Cultivar resistance to RAA was concluded to be related with the phenylpropanoid pathway; the isomerisations being the key metabolic reactions for a cultivar to be resistant or susceptible to RAA.In the present work, the phenolic profiles of the same set of samples were analysed by UHPLC-ESI(+)-QTOF/MS to use an untargeted metabolomic approach to identify biomarkers related to resistance to RAA. Apple phenolic fingerprints and their resistance to RAA were submitted to multivariate data analysis by Principal Component Analysis, Hierarchical Clustering Analysis, Partial Least Square-Discriminant Analysis (PLSDA) and Orthogonal-PLSDA (O-PLSDA), which disclosed that the main features related to resistant cultivars were due to 4-CQA, and those related to susceptible cultivars to 5-pCoQA. These results agreed with previous ones since both phenolics are involved in the isomerisation reactions mentioned above.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/173966
Untargeted metabolomic LC-MS fingerprinting of apple cultivars for the identification of biomarkers related to resistance to rosy apple aphid; XXX International Conference on Polyphenols; Turku; Finlandia; 2021; 283-284
2737-064X
2957-4056
CONICET Digital
CONICET
url http://hdl.handle.net/11336/173966
identifier_str_mv Untargeted metabolomic LC-MS fingerprinting of apple cultivars for the identification of biomarkers related to resistance to rosy apple aphid; XXX International Conference on Polyphenols; Turku; Finlandia; 2021; 283-284
2737-064X
2957-4056
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.groupepolyphenols.com/wp-content/uploads/2022/06/Polyphenols-Communications-e-Vol-1.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv International Association of Groupe Polyphenols
publisher.none.fl_str_mv International Association of Groupe Polyphenols
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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