Characterization of a phenol-degrading bacterium isolated from an industrial effluent and its potential application for bioremediation

Autores
Paisio, Cintia Elizabeth; Talano, Melina Andrea; González, Paola Solange; Pajuelo Domínguez, Eloisa; Agostini, Elizabeth
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of native microorganisms is a useful strategy for phenol bioremediation. In the present work, a bacterial strain, named RTE1.4, was isolated from effluents of a chemical industry. The strain was able to grow at high concentrations of phenol and its derivatives, such as guaiacol, 2,4-dichlorophenol and pentachlorophenol, as well as in a medium containing industrial effluents. This bacterium was identified as Acinetobacter sp. using morphological, physiological, biochemical and 16S rRNA gene analysis. Acinetobacter sp. RTE1.4 degraded phenol (200 to 600 mg/L) at wide pH range and temperature (5-9 and 25-37°C, respectively) demonstrating high adaptation ability to different conditions. The strain would metabolize phenol by the ortho-pathway since catechol 1,2-dioxygenase activity was detected. When bacteria were grown in medium containing phenol, an altered whole-cell protein pattern was observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), with the lack of some low-molecular mass polypeptides and an increase in the relative abundance of high-molecular mass proteins after treatment. Considering that the use of native strains in bioremediation studies shows several ecological advantages and that the studied bacterium showed high tolerance and biodegradation capabilities, Acinetobacter sp. RTE1.4 could be an appropriate microorganism for improving bioremediation and biotreatment of areas polluted with phenol and/or some of its derivatives. Moreover, the establishment of the optimal growth conditions (pH, temperature, concentration of the pollutant) would provide baseline data for bulk production of the strain and its use in bioremediation processes. © 2013 Copyright Taylor and Francis Group, LLC.
Fil: Paisio, Cintia Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Talano, Melina Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: González, Paola Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina
Fil: Pajuelo Domínguez, Eloisa. Universidad de Sevilla; España
Fil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Materia
CATECHOL 1,2-DIOXYGENASE
OPTIMAL GROWTH CONDITIONS
ORTHO PATHWAY
PHENOL REMOVAL
PROTEIN PATTERNS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195959
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Characterization of a phenol-degrading bacterium isolated from an industrial effluent and its potential application for bioremediationPaisio, Cintia ElizabethTalano, Melina AndreaGonzález, Paola SolangePajuelo Domínguez, EloisaAgostini, ElizabethCATECHOL 1,2-DIOXYGENASEOPTIMAL GROWTH CONDITIONSORTHO PATHWAYPHENOL REMOVALPROTEIN PATTERNShttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The use of native microorganisms is a useful strategy for phenol bioremediation. In the present work, a bacterial strain, named RTE1.4, was isolated from effluents of a chemical industry. The strain was able to grow at high concentrations of phenol and its derivatives, such as guaiacol, 2,4-dichlorophenol and pentachlorophenol, as well as in a medium containing industrial effluents. This bacterium was identified as Acinetobacter sp. using morphological, physiological, biochemical and 16S rRNA gene analysis. Acinetobacter sp. RTE1.4 degraded phenol (200 to 600 mg/L) at wide pH range and temperature (5-9 and 25-37°C, respectively) demonstrating high adaptation ability to different conditions. The strain would metabolize phenol by the ortho-pathway since catechol 1,2-dioxygenase activity was detected. When bacteria were grown in medium containing phenol, an altered whole-cell protein pattern was observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), with the lack of some low-molecular mass polypeptides and an increase in the relative abundance of high-molecular mass proteins after treatment. Considering that the use of native strains in bioremediation studies shows several ecological advantages and that the studied bacterium showed high tolerance and biodegradation capabilities, Acinetobacter sp. RTE1.4 could be an appropriate microorganism for improving bioremediation and biotreatment of areas polluted with phenol and/or some of its derivatives. Moreover, the establishment of the optimal growth conditions (pH, temperature, concentration of the pollutant) would provide baseline data for bulk production of the strain and its use in bioremediation processes. © 2013 Copyright Taylor and Francis Group, LLC.Fil: Paisio, Cintia Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Talano, Melina Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: González, Paola Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; ArgentinaFil: Pajuelo Domínguez, Eloisa. Universidad de Sevilla; EspañaFil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaTaylor & Francis Ltd2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195959Paisio, Cintia Elizabeth; Talano, Melina Andrea; González, Paola Solange; Pajuelo Domínguez, Eloisa; Agostini, Elizabeth; Characterization of a phenol-degrading bacterium isolated from an industrial effluent and its potential application for bioremediation; Taylor & Francis Ltd; Environmental Technology; 34; 4; 7-2012; 485-4930959-33301479-487XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/09593330.2012.701238info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/09593330.2012.701238info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:07:08Zoai:ri.conicet.gov.ar:11336/195959instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:07:08.48CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of a phenol-degrading bacterium isolated from an industrial effluent and its potential application for bioremediation
title Characterization of a phenol-degrading bacterium isolated from an industrial effluent and its potential application for bioremediation
spellingShingle Characterization of a phenol-degrading bacterium isolated from an industrial effluent and its potential application for bioremediation
Paisio, Cintia Elizabeth
CATECHOL 1,2-DIOXYGENASE
OPTIMAL GROWTH CONDITIONS
ORTHO PATHWAY
PHENOL REMOVAL
PROTEIN PATTERNS
title_short Characterization of a phenol-degrading bacterium isolated from an industrial effluent and its potential application for bioremediation
title_full Characterization of a phenol-degrading bacterium isolated from an industrial effluent and its potential application for bioremediation
title_fullStr Characterization of a phenol-degrading bacterium isolated from an industrial effluent and its potential application for bioremediation
title_full_unstemmed Characterization of a phenol-degrading bacterium isolated from an industrial effluent and its potential application for bioremediation
title_sort Characterization of a phenol-degrading bacterium isolated from an industrial effluent and its potential application for bioremediation
dc.creator.none.fl_str_mv Paisio, Cintia Elizabeth
Talano, Melina Andrea
González, Paola Solange
Pajuelo Domínguez, Eloisa
Agostini, Elizabeth
author Paisio, Cintia Elizabeth
author_facet Paisio, Cintia Elizabeth
Talano, Melina Andrea
González, Paola Solange
Pajuelo Domínguez, Eloisa
Agostini, Elizabeth
author_role author
author2 Talano, Melina Andrea
González, Paola Solange
Pajuelo Domínguez, Eloisa
Agostini, Elizabeth
author2_role author
author
author
author
dc.subject.none.fl_str_mv CATECHOL 1,2-DIOXYGENASE
OPTIMAL GROWTH CONDITIONS
ORTHO PATHWAY
PHENOL REMOVAL
PROTEIN PATTERNS
topic CATECHOL 1,2-DIOXYGENASE
OPTIMAL GROWTH CONDITIONS
ORTHO PATHWAY
PHENOL REMOVAL
PROTEIN PATTERNS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The use of native microorganisms is a useful strategy for phenol bioremediation. In the present work, a bacterial strain, named RTE1.4, was isolated from effluents of a chemical industry. The strain was able to grow at high concentrations of phenol and its derivatives, such as guaiacol, 2,4-dichlorophenol and pentachlorophenol, as well as in a medium containing industrial effluents. This bacterium was identified as Acinetobacter sp. using morphological, physiological, biochemical and 16S rRNA gene analysis. Acinetobacter sp. RTE1.4 degraded phenol (200 to 600 mg/L) at wide pH range and temperature (5-9 and 25-37°C, respectively) demonstrating high adaptation ability to different conditions. The strain would metabolize phenol by the ortho-pathway since catechol 1,2-dioxygenase activity was detected. When bacteria were grown in medium containing phenol, an altered whole-cell protein pattern was observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), with the lack of some low-molecular mass polypeptides and an increase in the relative abundance of high-molecular mass proteins after treatment. Considering that the use of native strains in bioremediation studies shows several ecological advantages and that the studied bacterium showed high tolerance and biodegradation capabilities, Acinetobacter sp. RTE1.4 could be an appropriate microorganism for improving bioremediation and biotreatment of areas polluted with phenol and/or some of its derivatives. Moreover, the establishment of the optimal growth conditions (pH, temperature, concentration of the pollutant) would provide baseline data for bulk production of the strain and its use in bioremediation processes. © 2013 Copyright Taylor and Francis Group, LLC.
Fil: Paisio, Cintia Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Talano, Melina Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: González, Paola Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Biología Molecular. Sección Química Biológica; Argentina
Fil: Pajuelo Domínguez, Eloisa. Universidad de Sevilla; España
Fil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
description The use of native microorganisms is a useful strategy for phenol bioremediation. In the present work, a bacterial strain, named RTE1.4, was isolated from effluents of a chemical industry. The strain was able to grow at high concentrations of phenol and its derivatives, such as guaiacol, 2,4-dichlorophenol and pentachlorophenol, as well as in a medium containing industrial effluents. This bacterium was identified as Acinetobacter sp. using morphological, physiological, biochemical and 16S rRNA gene analysis. Acinetobacter sp. RTE1.4 degraded phenol (200 to 600 mg/L) at wide pH range and temperature (5-9 and 25-37°C, respectively) demonstrating high adaptation ability to different conditions. The strain would metabolize phenol by the ortho-pathway since catechol 1,2-dioxygenase activity was detected. When bacteria were grown in medium containing phenol, an altered whole-cell protein pattern was observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), with the lack of some low-molecular mass polypeptides and an increase in the relative abundance of high-molecular mass proteins after treatment. Considering that the use of native strains in bioremediation studies shows several ecological advantages and that the studied bacterium showed high tolerance and biodegradation capabilities, Acinetobacter sp. RTE1.4 could be an appropriate microorganism for improving bioremediation and biotreatment of areas polluted with phenol and/or some of its derivatives. Moreover, the establishment of the optimal growth conditions (pH, temperature, concentration of the pollutant) would provide baseline data for bulk production of the strain and its use in bioremediation processes. © 2013 Copyright Taylor and Francis Group, LLC.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195959
Paisio, Cintia Elizabeth; Talano, Melina Andrea; González, Paola Solange; Pajuelo Domínguez, Eloisa; Agostini, Elizabeth; Characterization of a phenol-degrading bacterium isolated from an industrial effluent and its potential application for bioremediation; Taylor & Francis Ltd; Environmental Technology; 34; 4; 7-2012; 485-493
0959-3330
1479-487X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195959
identifier_str_mv Paisio, Cintia Elizabeth; Talano, Melina Andrea; González, Paola Solange; Pajuelo Domínguez, Eloisa; Agostini, Elizabeth; Characterization of a phenol-degrading bacterium isolated from an industrial effluent and its potential application for bioremediation; Taylor & Francis Ltd; Environmental Technology; 34; 4; 7-2012; 485-493
0959-3330
1479-487X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/09593330.2012.701238
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Taylor & Francis Ltd
publisher.none.fl_str_mv Taylor & Francis Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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