Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns

Autores
Moreno, Marta; Bickersmith, Sara; Harlow, Wesley; Hildebrandt, Jessica; Mckeon, Sascha N.; Silva Do Nascimento, Teresa Fernandes; Loaiza, Jose R.; Ruiz, Freddy; Lourenço de Oliveira, Ricardo; Sallum, María A. M.; Bergo, Eduardo S.; Fritz, Gary N.; Wilkerson, Richard C.; Dantur Juri, Maria Julia; Rangel, Yadira; Póvoa, Marinete M.; Gutiérrez Builes, Lina A.; Correa, Margarita M.; Conn, Jan E.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The molecular phylogenetic relationships and population structure of the species of the Anopheles triannulatus complex: Anopheles triannulatuss.s.,Anopheles halophylusand the putative species Anopheles triannulatus C were investigated. Methods:The mitochondrialCOIgene, the nuclearwhitegene and rDNA ITS2 of samples that include the known geographic distribution of these taxa were analyzed. Phylogenetic analyses were performed using Bayesian inference, Maximum parsimony and Maximum likelihood approaches. Results: Each data set analyzed separately yielded a different topology but none provided evidence for the separation ofAn. halophylusandAn. triannulatusC, consistent with the hypothesis that the two are undergoing incipient speciation. The phylogenetic analyses of the whitegene found three main clades, whereas the statistical parsimony network detected only a single metapopulation of Anopheles triannulatus s.l. Seven COI lineages were detected by phylogenetic and network analysis. In contrast, the network, but not the phylogenetic analyses,strongly supported three ITS2 groups. Combined data analyses provided the best resolution of the trees, with two major clades, Amazonian (clade I) and trans-Andean Amazon Delta(clade II). Clade I consists of multiple subclades: An. halophylus + An. triannulatus C; trans-Andean Venezuela; central Amazonia + central Bolivia; Atlantic coastal lowland; and Amazon delta. Clade II includes three subclades: Panama; cis-Andean Colombia; and cis-Venezuela. The Amazon delta specimens are in both clades, likely indicating local sympatry. Spatial and molecular variance analyses detected nine groups, corroborating some of subclades obtained in the combined data analysis. Conclusion:Combination of the three molecular markers provided the best resolution for differentiation within An.triannulatus s.s. and An. halophylusand C. The latest two species seem to be very closely related and the analyses performed were not conclusive regarding species differentiation. Further studies including new molecular markers would be desirable to solve this species status question. Besides, results of the study indicate a trans-Andean origin for An. triannulatus s.l. The potential implications for malaria epidemiology remain to be investigated.
Fil: Moreno, Marta. New York State Department of Health. Wadsworth Center; Estados Unidos;
Fil: Bickersmith, Sara. New York State Department of Health. Wadsworth Center; Estados Unidos;
Fil: Harlow, Wesley. New York State Department of Health. Wadsworth Center; Estados Unidos;
Fil: Hildebrandt, Jessica. New York State Department of Health. Wadsworth Center; Estados Unidos;
Fil: Mckeon, Sascha N.. New York State Department of Health. Wadsworth Center; Estados Unidos; State University of New York. School of Public Health. Department of Biomedical Sciences; Estados Unidos;
Fil: Silva Do Nascimento, Teresa Fernandes. Ministerio de Salud de Brasil. Fundacion Oswaldo Cruz. Departamento de Entomología; Brasil;
Fil: Loaiza, Jose R.. Instituto de Investigaciones Científicas y Servicios de Alta Tecnología; Panamá;
Fil: Ruiz, Freddy. Walter Reed Army Institute of Research. Division of Entomology; Estados Unidos;
Fil: Lourenço de Oliveira, Ricardo. Ministerio de Salud de Brasil. Fundacion Oswaldo Cruz. Departamento de Entomología; Brasil;
Fil: Sallum, María A. M.. Ministerio de Salud de Brasil. Fundacion Oswaldo Cruz. Departamento de Entomología; Brasil;
Fil: Bergo, Eduardo S.. Superintendência de Controle de Endemias; Brazil;
Fil: Fritz, Gary N.. Eastern Illinois University. Department of Biological Sciences; Estados Unidos;
Fil: Wilkerson, Richard C.. Walter Reed Army Institute of Research. Division of Entomology; Estados Unidos;
Fil: Dantur Juri, Maria Julia. Fundación Miguel Lillo. Dirección de Zoología; Argentina; Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Instituto Superior de Entomología; Argentina;
Fil: Rangel, Yadira. Universidad Central de Venezuela. Instituto de Zoología y Ecología Tropical. Laboratorio de Biología de Vectores; Venezuela;
Fil: Póvoa, Marinete M.. Instituto Evandro Chagas. Secção de Parasitologia; Brasil;
Fil: Gutiérrez Builes, Lina A.. Universidad de Antioquia. Escuela de Microbiología. Grupo de Microbiología Molecular; Colombia;
Fil: Correa, Margarita M.. Universidad de Antioquia. Escuela de Microbiología. Grupo de Microbiología Molecular; Colombia;
Fil: Conn, Jan E.. New York State Department of Health. Wadsworth Center; Estados Unidos;
Materia
ANOPHELES TRIANNULATUS S S.
ANOPHELES HALOPHYLUS
PHYLOGEOGRAPHY
COI GENE
WHITE GENE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1575

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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patternsMoreno, MartaBickersmith, SaraHarlow, WesleyHildebrandt, JessicaMckeon, Sascha N.Silva Do Nascimento, Teresa FernandesLoaiza, Jose R.Ruiz, FreddyLourenço de Oliveira, RicardoSallum, María A. M.Bergo, Eduardo S.Fritz, Gary N.Wilkerson, Richard C.Dantur Juri, Maria JuliaRangel, YadiraPóvoa, Marinete M.Gutiérrez Builes, Lina A.Correa, Margarita M.Conn, Jan E.ANOPHELES TRIANNULATUS S S.ANOPHELES HALOPHYLUSPHYLOGEOGRAPHYCOI GENEWHITE GENEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The molecular phylogenetic relationships and population structure of the species of the Anopheles triannulatus complex: Anopheles triannulatuss.s.,Anopheles halophylusand the putative species Anopheles triannulatus C were investigated. Methods:The mitochondrialCOIgene, the nuclearwhitegene and rDNA ITS2 of samples that include the known geographic distribution of these taxa were analyzed. Phylogenetic analyses were performed using Bayesian inference, Maximum parsimony and Maximum likelihood approaches. Results: Each data set analyzed separately yielded a different topology but none provided evidence for the separation ofAn. halophylusandAn. triannulatusC, consistent with the hypothesis that the two are undergoing incipient speciation. The phylogenetic analyses of the whitegene found three main clades, whereas the statistical parsimony network detected only a single metapopulation of Anopheles triannulatus s.l. Seven COI lineages were detected by phylogenetic and network analysis. In contrast, the network, but not the phylogenetic analyses,strongly supported three ITS2 groups. Combined data analyses provided the best resolution of the trees, with two major clades, Amazonian (clade I) and trans-Andean Amazon Delta(clade II). Clade I consists of multiple subclades: An. halophylus + An. triannulatus C; trans-Andean Venezuela; central Amazonia + central Bolivia; Atlantic coastal lowland; and Amazon delta. Clade II includes three subclades: Panama; cis-Andean Colombia; and cis-Venezuela. The Amazon delta specimens are in both clades, likely indicating local sympatry. Spatial and molecular variance analyses detected nine groups, corroborating some of subclades obtained in the combined data analysis. Conclusion:Combination of the three molecular markers provided the best resolution for differentiation within An.triannulatus s.s. and An. halophylusand C. The latest two species seem to be very closely related and the analyses performed were not conclusive regarding species differentiation. Further studies including new molecular markers would be desirable to solve this species status question. Besides, results of the study indicate a trans-Andean origin for An. triannulatus s.l. The potential implications for malaria epidemiology remain to be investigated.Fil: Moreno, Marta. New York State Department of Health. Wadsworth Center; Estados Unidos;Fil: Bickersmith, Sara. New York State Department of Health. Wadsworth Center; Estados Unidos;Fil: Harlow, Wesley. New York State Department of Health. Wadsworth Center; Estados Unidos;Fil: Hildebrandt, Jessica. New York State Department of Health. Wadsworth Center; Estados Unidos;Fil: Mckeon, Sascha N.. New York State Department of Health. Wadsworth Center; Estados Unidos; State University of New York. School of Public Health. Department of Biomedical Sciences; Estados Unidos;Fil: Silva Do Nascimento, Teresa Fernandes. Ministerio de Salud de Brasil. Fundacion Oswaldo Cruz. Departamento de Entomología; Brasil;Fil: Loaiza, Jose R.. Instituto de Investigaciones Científicas y Servicios de Alta Tecnología; Panamá;Fil: Ruiz, Freddy. Walter Reed Army Institute of Research. Division of Entomology; Estados Unidos;Fil: Lourenço de Oliveira, Ricardo. Ministerio de Salud de Brasil. Fundacion Oswaldo Cruz. Departamento de Entomología; Brasil;Fil: Sallum, María A. M.. Ministerio de Salud de Brasil. Fundacion Oswaldo Cruz. Departamento de Entomología; Brasil;Fil: Bergo, Eduardo S.. Superintendência de Controle de Endemias; Brazil;Fil: Fritz, Gary N.. Eastern Illinois University. Department of Biological Sciences; Estados Unidos;Fil: Wilkerson, Richard C.. Walter Reed Army Institute of Research. Division of Entomology; Estados Unidos;Fil: Dantur Juri, Maria Julia. Fundación Miguel Lillo. Dirección de Zoología; Argentina; Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Instituto Superior de Entomología; Argentina;Fil: Rangel, Yadira. Universidad Central de Venezuela. Instituto de Zoología y Ecología Tropical. Laboratorio de Biología de Vectores; Venezuela;Fil: Póvoa, Marinete M.. Instituto Evandro Chagas. Secção de Parasitologia; Brasil;Fil: Gutiérrez Builes, Lina A.. Universidad de Antioquia. Escuela de Microbiología. Grupo de Microbiología Molecular; Colombia;Fil: Correa, Margarita M.. Universidad de Antioquia. Escuela de Microbiología. Grupo de Microbiología Molecular; Colombia;Fil: Conn, Jan E.. New York State Department of Health. Wadsworth Center; Estados Unidos;Biomed Central2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1575Moreno, Marta; Bickersmith, Sara; Harlow, Wesley; Hildebrandt, Jessica; Mckeon, Sascha N.; et al.; Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns; Biomed Central; Parasites And Vectors; 6; 47; 2-2013; 1-171756-3305enginfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606328/info:eu-repo/semantics/altIdentifier/url/http://www.parasitesandvectors.com/content/6/1/47info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:37:36Zoai:ri.conicet.gov.ar:11336/1575instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:37:36.984CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns
title Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns
spellingShingle Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns
Moreno, Marta
ANOPHELES TRIANNULATUS S S.
ANOPHELES HALOPHYLUS
PHYLOGEOGRAPHY
COI GENE
WHITE GENE
title_short Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns
title_full Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns
title_fullStr Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns
title_full_unstemmed Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns
title_sort Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns
dc.creator.none.fl_str_mv Moreno, Marta
Bickersmith, Sara
Harlow, Wesley
Hildebrandt, Jessica
Mckeon, Sascha N.
Silva Do Nascimento, Teresa Fernandes
Loaiza, Jose R.
Ruiz, Freddy
Lourenço de Oliveira, Ricardo
Sallum, María A. M.
Bergo, Eduardo S.
Fritz, Gary N.
Wilkerson, Richard C.
Dantur Juri, Maria Julia
Rangel, Yadira
Póvoa, Marinete M.
Gutiérrez Builes, Lina A.
Correa, Margarita M.
Conn, Jan E.
author Moreno, Marta
author_facet Moreno, Marta
Bickersmith, Sara
Harlow, Wesley
Hildebrandt, Jessica
Mckeon, Sascha N.
Silva Do Nascimento, Teresa Fernandes
Loaiza, Jose R.
Ruiz, Freddy
Lourenço de Oliveira, Ricardo
Sallum, María A. M.
Bergo, Eduardo S.
Fritz, Gary N.
Wilkerson, Richard C.
Dantur Juri, Maria Julia
Rangel, Yadira
Póvoa, Marinete M.
Gutiérrez Builes, Lina A.
Correa, Margarita M.
Conn, Jan E.
author_role author
author2 Bickersmith, Sara
Harlow, Wesley
Hildebrandt, Jessica
Mckeon, Sascha N.
Silva Do Nascimento, Teresa Fernandes
Loaiza, Jose R.
Ruiz, Freddy
Lourenço de Oliveira, Ricardo
Sallum, María A. M.
Bergo, Eduardo S.
Fritz, Gary N.
Wilkerson, Richard C.
Dantur Juri, Maria Julia
Rangel, Yadira
Póvoa, Marinete M.
Gutiérrez Builes, Lina A.
Correa, Margarita M.
Conn, Jan E.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANOPHELES TRIANNULATUS S S.
ANOPHELES HALOPHYLUS
PHYLOGEOGRAPHY
COI GENE
WHITE GENE
topic ANOPHELES TRIANNULATUS S S.
ANOPHELES HALOPHYLUS
PHYLOGEOGRAPHY
COI GENE
WHITE GENE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The molecular phylogenetic relationships and population structure of the species of the Anopheles triannulatus complex: Anopheles triannulatuss.s.,Anopheles halophylusand the putative species Anopheles triannulatus C were investigated. Methods:The mitochondrialCOIgene, the nuclearwhitegene and rDNA ITS2 of samples that include the known geographic distribution of these taxa were analyzed. Phylogenetic analyses were performed using Bayesian inference, Maximum parsimony and Maximum likelihood approaches. Results: Each data set analyzed separately yielded a different topology but none provided evidence for the separation ofAn. halophylusandAn. triannulatusC, consistent with the hypothesis that the two are undergoing incipient speciation. The phylogenetic analyses of the whitegene found three main clades, whereas the statistical parsimony network detected only a single metapopulation of Anopheles triannulatus s.l. Seven COI lineages were detected by phylogenetic and network analysis. In contrast, the network, but not the phylogenetic analyses,strongly supported three ITS2 groups. Combined data analyses provided the best resolution of the trees, with two major clades, Amazonian (clade I) and trans-Andean Amazon Delta(clade II). Clade I consists of multiple subclades: An. halophylus + An. triannulatus C; trans-Andean Venezuela; central Amazonia + central Bolivia; Atlantic coastal lowland; and Amazon delta. Clade II includes three subclades: Panama; cis-Andean Colombia; and cis-Venezuela. The Amazon delta specimens are in both clades, likely indicating local sympatry. Spatial and molecular variance analyses detected nine groups, corroborating some of subclades obtained in the combined data analysis. Conclusion:Combination of the three molecular markers provided the best resolution for differentiation within An.triannulatus s.s. and An. halophylusand C. The latest two species seem to be very closely related and the analyses performed were not conclusive regarding species differentiation. Further studies including new molecular markers would be desirable to solve this species status question. Besides, results of the study indicate a trans-Andean origin for An. triannulatus s.l. The potential implications for malaria epidemiology remain to be investigated.
Fil: Moreno, Marta. New York State Department of Health. Wadsworth Center; Estados Unidos;
Fil: Bickersmith, Sara. New York State Department of Health. Wadsworth Center; Estados Unidos;
Fil: Harlow, Wesley. New York State Department of Health. Wadsworth Center; Estados Unidos;
Fil: Hildebrandt, Jessica. New York State Department of Health. Wadsworth Center; Estados Unidos;
Fil: Mckeon, Sascha N.. New York State Department of Health. Wadsworth Center; Estados Unidos; State University of New York. School of Public Health. Department of Biomedical Sciences; Estados Unidos;
Fil: Silva Do Nascimento, Teresa Fernandes. Ministerio de Salud de Brasil. Fundacion Oswaldo Cruz. Departamento de Entomología; Brasil;
Fil: Loaiza, Jose R.. Instituto de Investigaciones Científicas y Servicios de Alta Tecnología; Panamá;
Fil: Ruiz, Freddy. Walter Reed Army Institute of Research. Division of Entomology; Estados Unidos;
Fil: Lourenço de Oliveira, Ricardo. Ministerio de Salud de Brasil. Fundacion Oswaldo Cruz. Departamento de Entomología; Brasil;
Fil: Sallum, María A. M.. Ministerio de Salud de Brasil. Fundacion Oswaldo Cruz. Departamento de Entomología; Brasil;
Fil: Bergo, Eduardo S.. Superintendência de Controle de Endemias; Brazil;
Fil: Fritz, Gary N.. Eastern Illinois University. Department of Biological Sciences; Estados Unidos;
Fil: Wilkerson, Richard C.. Walter Reed Army Institute of Research. Division of Entomology; Estados Unidos;
Fil: Dantur Juri, Maria Julia. Fundación Miguel Lillo. Dirección de Zoología; Argentina; Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Instituto Superior de Entomología; Argentina;
Fil: Rangel, Yadira. Universidad Central de Venezuela. Instituto de Zoología y Ecología Tropical. Laboratorio de Biología de Vectores; Venezuela;
Fil: Póvoa, Marinete M.. Instituto Evandro Chagas. Secção de Parasitologia; Brasil;
Fil: Gutiérrez Builes, Lina A.. Universidad de Antioquia. Escuela de Microbiología. Grupo de Microbiología Molecular; Colombia;
Fil: Correa, Margarita M.. Universidad de Antioquia. Escuela de Microbiología. Grupo de Microbiología Molecular; Colombia;
Fil: Conn, Jan E.. New York State Department of Health. Wadsworth Center; Estados Unidos;
description The molecular phylogenetic relationships and population structure of the species of the Anopheles triannulatus complex: Anopheles triannulatuss.s.,Anopheles halophylusand the putative species Anopheles triannulatus C were investigated. Methods:The mitochondrialCOIgene, the nuclearwhitegene and rDNA ITS2 of samples that include the known geographic distribution of these taxa were analyzed. Phylogenetic analyses were performed using Bayesian inference, Maximum parsimony and Maximum likelihood approaches. Results: Each data set analyzed separately yielded a different topology but none provided evidence for the separation ofAn. halophylusandAn. triannulatusC, consistent with the hypothesis that the two are undergoing incipient speciation. The phylogenetic analyses of the whitegene found three main clades, whereas the statistical parsimony network detected only a single metapopulation of Anopheles triannulatus s.l. Seven COI lineages were detected by phylogenetic and network analysis. In contrast, the network, but not the phylogenetic analyses,strongly supported three ITS2 groups. Combined data analyses provided the best resolution of the trees, with two major clades, Amazonian (clade I) and trans-Andean Amazon Delta(clade II). Clade I consists of multiple subclades: An. halophylus + An. triannulatus C; trans-Andean Venezuela; central Amazonia + central Bolivia; Atlantic coastal lowland; and Amazon delta. Clade II includes three subclades: Panama; cis-Andean Colombia; and cis-Venezuela. The Amazon delta specimens are in both clades, likely indicating local sympatry. Spatial and molecular variance analyses detected nine groups, corroborating some of subclades obtained in the combined data analysis. Conclusion:Combination of the three molecular markers provided the best resolution for differentiation within An.triannulatus s.s. and An. halophylusand C. The latest two species seem to be very closely related and the analyses performed were not conclusive regarding species differentiation. Further studies including new molecular markers would be desirable to solve this species status question. Besides, results of the study indicate a trans-Andean origin for An. triannulatus s.l. The potential implications for malaria epidemiology remain to be investigated.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1575
Moreno, Marta; Bickersmith, Sara; Harlow, Wesley; Hildebrandt, Jessica; Mckeon, Sascha N.; et al.; Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns; Biomed Central; Parasites And Vectors; 6; 47; 2-2013; 1-17
1756-3305
url http://hdl.handle.net/11336/1575
identifier_str_mv Moreno, Marta; Bickersmith, Sara; Harlow, Wesley; Hildebrandt, Jessica; Mckeon, Sascha N.; et al.; Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns; Biomed Central; Parasites And Vectors; 6; 47; 2-2013; 1-17
1756-3305
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3606328/
info:eu-repo/semantics/altIdentifier/url/http://www.parasitesandvectors.com/content/6/1/47
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Biomed Central
publisher.none.fl_str_mv Biomed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432