Assessment of cellulase complex secretory capacity of trichoderma strains and morphological and molecular identification of the isolate with the highest enzymatic secretion capacit...

Autores: Castrillo, María Lorena; Bich, Gustavo Angel; Amerio, Natalia Soledad; Rodríguez, María Daniela; Zapata, Pedro Dario; Villalba, Laura Lidia
Año de publicación: 2021
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: The bioconversion of lignocellulosic biomass into monomeric sugars is a key economic difficulty hindering the profitable use of plant biomass as energy. The production of cellulase is a main factor in the cellulose hydrolysis. Among the main cellulase producers are the filamentous fungi. Therefore, many efforts have been made in obtaining new microorganisms with high cellulase secretion capacity. The cellulase secretory capacity of 28 isolates of Trichoderma was qualitatively and quantitatively evaluated. The detection of cellulolytic fungi was correlated with both Congo red and the dinitrosalicylic acid reagent methods. Based on qualitatively assays, sixteen of the isolates revealed carboxymethyl cellulose degradation ability, where the Trichoderma POS7 isolate showed the highest increase in filter paper activity, endo-1,4-β-glucanases and β-glucosidases activities (p<0.05) in a short incubation time. This isolate was molecularly identified as Trichoderma koningiopsis, based on internal transcribed spacer sequences. Our results provide new information and reveal new microorganism in the hydrolysis of cellulose material. The phylogenetic analysis revealed close positioning of T. koningiopsis clade with T. viride, T. viridescens and T. petersenii clades in a closely related group, in concordance with the current taxonomic classification of Trichoderma genus.
Fil: Castrillo, María Lorena. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Bich, Gustavo Angel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Amerio, Natalia Soledad. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Rodríguez, María Daniela. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Materia: FUNGAL ISOLATE
CELLULASE ACTIVITY
MORPHOLOGYCAL CHARACTERS
RIBOSOMAL DNA
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nd/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/154920

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spelling	Assessment of cellulase complex secretory capacity of trichoderma strains and morphological and molecular identification of the isolate with the highest enzymatic secretion capacityCastrillo, María LorenaBich, Gustavo AngelAmerio, Natalia SoledadRodríguez, María DanielaZapata, Pedro DarioVillalba, Laura LidiaFUNGAL ISOLATECELLULASE ACTIVITYMORPHOLOGYCAL CHARACTERSRIBOSOMAL DNAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The bioconversion of lignocellulosic biomass into monomeric sugars is a key economic difficulty hindering the profitable use of plant biomass as energy. The production of cellulase is a main factor in the cellulose hydrolysis. Among the main cellulase producers are the filamentous fungi. Therefore, many efforts have been made in obtaining new microorganisms with high cellulase secretion capacity. The cellulase secretory capacity of 28 isolates of Trichoderma was qualitatively and quantitatively evaluated. The detection of cellulolytic fungi was correlated with both Congo red and the dinitrosalicylic acid reagent methods. Based on qualitatively assays, sixteen of the isolates revealed carboxymethyl cellulose degradation ability, where the Trichoderma POS7 isolate showed the highest increase in filter paper activity, endo-1,4-β-glucanases and β-glucosidases activities (p<0.05) in a short incubation time. This isolate was molecularly identified as Trichoderma koningiopsis, based on internal transcribed spacer sequences. Our results provide new information and reveal new microorganism in the hydrolysis of cellulose material. The phylogenetic analysis revealed close positioning of T. koningiopsis clade with T. viride, T. viridescens and T. petersenii clades in a closely related group, in concordance with the current taxonomic classification of Trichoderma genus.Fil: Castrillo, María Lorena. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Bich, Gustavo Angel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Amerio, Natalia Soledad. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Rodríguez, María Daniela. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaSlovak University of Agriculture. Faculty of Biotechnology and Food Sciences2021-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/154920Castrillo, María Lorena; Bich, Gustavo Angel; Amerio, Natalia Soledad; Rodríguez, María Daniela; Zapata, Pedro Dario; et al.; Assessment of cellulase complex secretory capacity of trichoderma strains and morphological and molecular identification of the isolate with the highest enzymatic secretion capacity; Slovak University of Agriculture. Faculty of Biotechnology and Food Sciences; Journal of Microbiology, Biotechnology and Food Sciences; 10; 5; 4-2021; 1-131338-5178CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://office2.jmbfs.org/index.php/JMBFS/article/view/1357info:eu-repo/semantics/altIdentifier/doi/10.15414/jmbfs.1357info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:55:31Zoai:ri.conicet.gov.ar:11336/154920instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:55:32.283CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Assessment of cellulase complex secretory capacity of trichoderma strains and morphological and molecular identification of the isolate with the highest enzymatic secretion capacity
title	Assessment of cellulase complex secretory capacity of trichoderma strains and morphological and molecular identification of the isolate with the highest enzymatic secretion capacity
spellingShingle	Assessment of cellulase complex secretory capacity of trichoderma strains and morphological and molecular identification of the isolate with the highest enzymatic secretion capacity Castrillo, María Lorena FUNGAL ISOLATE CELLULASE ACTIVITY MORPHOLOGYCAL CHARACTERS RIBOSOMAL DNA
title_short	Assessment of cellulase complex secretory capacity of trichoderma strains and morphological and molecular identification of the isolate with the highest enzymatic secretion capacity
title_full	Assessment of cellulase complex secretory capacity of trichoderma strains and morphological and molecular identification of the isolate with the highest enzymatic secretion capacity
title_fullStr	Assessment of cellulase complex secretory capacity of trichoderma strains and morphological and molecular identification of the isolate with the highest enzymatic secretion capacity
title_full_unstemmed	Assessment of cellulase complex secretory capacity of trichoderma strains and morphological and molecular identification of the isolate with the highest enzymatic secretion capacity
title_sort	Assessment of cellulase complex secretory capacity of trichoderma strains and morphological and molecular identification of the isolate with the highest enzymatic secretion capacity
dc.creator.none.fl_str_mv	Castrillo, María Lorena Bich, Gustavo Angel Amerio, Natalia Soledad Rodríguez, María Daniela Zapata, Pedro Dario Villalba, Laura Lidia
author	Castrillo, María Lorena
author_facet	Castrillo, María Lorena Bich, Gustavo Angel Amerio, Natalia Soledad Rodríguez, María Daniela Zapata, Pedro Dario Villalba, Laura Lidia
author_role	author
author2	Bich, Gustavo Angel Amerio, Natalia Soledad Rodríguez, María Daniela Zapata, Pedro Dario Villalba, Laura Lidia
author2_role	author author author author author
dc.subject.none.fl_str_mv	FUNGAL ISOLATE CELLULASE ACTIVITY MORPHOLOGYCAL CHARACTERS RIBOSOMAL DNA
topic	FUNGAL ISOLATE CELLULASE ACTIVITY MORPHOLOGYCAL CHARACTERS RIBOSOMAL DNA
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	The bioconversion of lignocellulosic biomass into monomeric sugars is a key economic difficulty hindering the profitable use of plant biomass as energy. The production of cellulase is a main factor in the cellulose hydrolysis. Among the main cellulase producers are the filamentous fungi. Therefore, many efforts have been made in obtaining new microorganisms with high cellulase secretion capacity. The cellulase secretory capacity of 28 isolates of Trichoderma was qualitatively and quantitatively evaluated. The detection of cellulolytic fungi was correlated with both Congo red and the dinitrosalicylic acid reagent methods. Based on qualitatively assays, sixteen of the isolates revealed carboxymethyl cellulose degradation ability, where the Trichoderma POS7 isolate showed the highest increase in filter paper activity, endo-1,4-β-glucanases and β-glucosidases activities (p<0.05) in a short incubation time. This isolate was molecularly identified as Trichoderma koningiopsis, based on internal transcribed spacer sequences. Our results provide new information and reveal new microorganism in the hydrolysis of cellulose material. The phylogenetic analysis revealed close positioning of T. koningiopsis clade with T. viride, T. viridescens and T. petersenii clades in a closely related group, in concordance with the current taxonomic classification of Trichoderma genus. Fil: Castrillo, María Lorena. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina Fil: Bich, Gustavo Angel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina Fil: Amerio, Natalia Soledad. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina Fil: Rodríguez, María Daniela. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina Fil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
description	The bioconversion of lignocellulosic biomass into monomeric sugars is a key economic difficulty hindering the profitable use of plant biomass as energy. The production of cellulase is a main factor in the cellulose hydrolysis. Among the main cellulase producers are the filamentous fungi. Therefore, many efforts have been made in obtaining new microorganisms with high cellulase secretion capacity. The cellulase secretory capacity of 28 isolates of Trichoderma was qualitatively and quantitatively evaluated. The detection of cellulolytic fungi was correlated with both Congo red and the dinitrosalicylic acid reagent methods. Based on qualitatively assays, sixteen of the isolates revealed carboxymethyl cellulose degradation ability, where the Trichoderma POS7 isolate showed the highest increase in filter paper activity, endo-1,4-β-glucanases and β-glucosidases activities (p<0.05) in a short incubation time. This isolate was molecularly identified as Trichoderma koningiopsis, based on internal transcribed spacer sequences. Our results provide new information and reveal new microorganism in the hydrolysis of cellulose material. The phylogenetic analysis revealed close positioning of T. koningiopsis clade with T. viride, T. viridescens and T. petersenii clades in a closely related group, in concordance with the current taxonomic classification of Trichoderma genus.
publishDate	2021
dc.date.none.fl_str_mv	2021-04
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/154920 Castrillo, María Lorena; Bich, Gustavo Angel; Amerio, Natalia Soledad; Rodríguez, María Daniela; Zapata, Pedro Dario; et al.; Assessment of cellulase complex secretory capacity of trichoderma strains and morphological and molecular identification of the isolate with the highest enzymatic secretion capacity; Slovak University of Agriculture. Faculty of Biotechnology and Food Sciences; Journal of Microbiology, Biotechnology and Food Sciences; 10; 5; 4-2021; 1-13 1338-5178 CONICET Digital CONICET
url	http://hdl.handle.net/11336/154920
identifier_str_mv	Castrillo, María Lorena; Bich, Gustavo Angel; Amerio, Natalia Soledad; Rodríguez, María Daniela; Zapata, Pedro Dario; et al.; Assessment of cellulase complex secretory capacity of trichoderma strains and morphological and molecular identification of the isolate with the highest enzymatic secretion capacity; Slovak University of Agriculture. Faculty of Biotechnology and Food Sciences; Journal of Microbiology, Biotechnology and Food Sciences; 10; 5; 4-2021; 1-13 1338-5178 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/https://office2.jmbfs.org/index.php/JMBFS/article/view/1357 info:eu-repo/semantics/altIdentifier/doi/10.15414/jmbfs.1357
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nd/2.5/ar/
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rights_invalid_str_mv	https://creativecommons.org/licenses/by-nd/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	Slovak University of Agriculture. Faculty of Biotechnology and Food Sciences
publisher.none.fl_str_mv	Slovak University of Agriculture. Faculty of Biotechnology and Food Sciences
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Assessment of cellulase complex secretory capacity of trichoderma strains and morphological and molecular identification of the isolate with the highest enzymatic secretion capacit...

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