Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology

Autores
Cortella, Lucas R. X.; Cestari, Idágene A.; Lahuerta, Ricardo D.; Arana, Matheus C.; Soldera, Marcos Maximiliano; Rank, Andreas; Lasagni, Andrés F.; Cestari, Ismar N.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Surface functionalization of polymers aims to introduce novel properties that favor bioactive responses. We have investigated the possibility of surface functionalization of polyethylene terephthalate (PET) sheets by the combination of laser ablation with hot embossing and the application of such techniques in the field of stem cell research. We investigated the response of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to topography in the low micrometer range. HiPSC-CMs are expected to offer new therapeutic tools for myocardial replacement or regeneration after an infarct or other causes of cardiac tissue loss. However, hiPSC-CMs are phenotypically immature compared to myocytes in the adult myocardium, hampering their clinical application. We aimed to develop and test a high-throughput technique for surface structuring that would improve hiPSC-CMs structural maturation. We used laser ablation with a ps-laser source in combination with nanoimprint lithography to fabricate large areas of homogeneous micron- to submicron line-like pattern with a spatial period of 3 µm on the PET surface. We evaluated cell morphology, alignment, sarcomeric myofibrils assembly, and calcium transients to evaluate phenotypic changes associated with culturing hiPSC-CMs on functionalized PET. Surface functionalization through hot embossing was able to generate, at low cost, low micrometer features on the PET surface that influenced the hiPSC-CMs phenotype, suggesting improved structural and functional maturation. This technique may be relevant for high-throughput technologies that require conditioning of hiPSC-CMs and may be useful for the production of these cells for drug screening and disease modeling applications with lower costs.
Fil: Cortella, Lucas R. X.. Universidade de Sao Paulo; Brasil
Fil: Cestari, Idágene A.. Universidade de Sao Paulo; Brasil
Fil: Lahuerta, Ricardo D.. Universidade de Sao Paulo; Brasil
Fil: Arana, Matheus C.. Universidade de Sao Paulo; Brasil
Fil: Soldera, Marcos Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina
Fil: Rank, Andreas. Technische Universität Dresden; Alemania
Fil: Lasagni, Andrés F.. Technische Universität Dresden; Alemania
Fil: Cestari, Ismar N.. Universidade de Sao Paulo; Brasil
Materia
CARDIOMYOCYTES
DIRECT LASER INTERFERENCE PATTERNING
HIPSC
POLYETHYLENE TEREPHTHALATE
ROLL-TO-ROLL NANOIMPRINT LITHOGRAPHY
SURFACE TOPOGRAPHY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/183417
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/183417
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technologyCortella, Lucas R. X.Cestari, Idágene A.Lahuerta, Ricardo D.Arana, Matheus C.Soldera, Marcos MaximilianoRank, AndreasLasagni, Andrés F.Cestari, Ismar N.CARDIOMYOCYTESDIRECT LASER INTERFERENCE PATTERNINGHIPSCPOLYETHYLENE TEREPHTHALATEROLL-TO-ROLL NANOIMPRINT LITHOGRAPHYSURFACE TOPOGRAPHYhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Surface functionalization of polymers aims to introduce novel properties that favor bioactive responses. We have investigated the possibility of surface functionalization of polyethylene terephthalate (PET) sheets by the combination of laser ablation with hot embossing and the application of such techniques in the field of stem cell research. We investigated the response of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to topography in the low micrometer range. HiPSC-CMs are expected to offer new therapeutic tools for myocardial replacement or regeneration after an infarct or other causes of cardiac tissue loss. However, hiPSC-CMs are phenotypically immature compared to myocytes in the adult myocardium, hampering their clinical application. We aimed to develop and test a high-throughput technique for surface structuring that would improve hiPSC-CMs structural maturation. We used laser ablation with a ps-laser source in combination with nanoimprint lithography to fabricate large areas of homogeneous micron- to submicron line-like pattern with a spatial period of 3 µm on the PET surface. We evaluated cell morphology, alignment, sarcomeric myofibrils assembly, and calcium transients to evaluate phenotypic changes associated with culturing hiPSC-CMs on functionalized PET. Surface functionalization through hot embossing was able to generate, at low cost, low micrometer features on the PET surface that influenced the hiPSC-CMs phenotype, suggesting improved structural and functional maturation. This technique may be relevant for high-throughput technologies that require conditioning of hiPSC-CMs and may be useful for the production of these cells for drug screening and disease modeling applications with lower costs.Fil: Cortella, Lucas R. X.. Universidade de Sao Paulo; BrasilFil: Cestari, Idágene A.. Universidade de Sao Paulo; BrasilFil: Lahuerta, Ricardo D.. Universidade de Sao Paulo; BrasilFil: Arana, Matheus C.. Universidade de Sao Paulo; BrasilFil: Soldera, Marcos Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; ArgentinaFil: Rank, Andreas. Technische Universität Dresden; AlemaniaFil: Lasagni, Andrés F.. Technische Universität Dresden; AlemaniaFil: Cestari, Ismar N.. Universidade de Sao Paulo; BrasilIOP Publishing2021-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/183417Cortella, Lucas R. X.; Cestari, Idágene A.; Lahuerta, Ricardo D.; Arana, Matheus C.; Soldera, Marcos Maximiliano; et al.; Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology; IOP Publishing; Biomedical Materials; 16; 6; 8-2021; 1-391748-6041CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1088/1748-605X/ac1f73info:eu-repo/semantics/altIdentifier/doi/10.1088/1748-605X/ac1f73info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:04Zoai:ri.conicet.gov.ar:11336/183417instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:05.088CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology
title Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology
spellingShingle Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology
Cortella, Lucas R. X.
CARDIOMYOCYTES
DIRECT LASER INTERFERENCE PATTERNING
HIPSC
POLYETHYLENE TEREPHTHALATE
ROLL-TO-ROLL NANOIMPRINT LITHOGRAPHY
SURFACE TOPOGRAPHY
title_short Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology
title_full Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology
title_fullStr Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology
title_full_unstemmed Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology
title_sort Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology
dc.creator.none.fl_str_mv Cortella, Lucas R. X.
Cestari, Idágene A.
Lahuerta, Ricardo D.
Arana, Matheus C.
Soldera, Marcos Maximiliano
Rank, Andreas
Lasagni, Andrés F.
Cestari, Ismar N.
author Cortella, Lucas R. X.
author_facet Cortella, Lucas R. X.
Cestari, Idágene A.
Lahuerta, Ricardo D.
Arana, Matheus C.
Soldera, Marcos Maximiliano
Rank, Andreas
Lasagni, Andrés F.
Cestari, Ismar N.
author_role author
author2 Cestari, Idágene A.
Lahuerta, Ricardo D.
Arana, Matheus C.
Soldera, Marcos Maximiliano
Rank, Andreas
Lasagni, Andrés F.
Cestari, Ismar N.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CARDIOMYOCYTES
DIRECT LASER INTERFERENCE PATTERNING
HIPSC
POLYETHYLENE TEREPHTHALATE
ROLL-TO-ROLL NANOIMPRINT LITHOGRAPHY
SURFACE TOPOGRAPHY
topic CARDIOMYOCYTES
DIRECT LASER INTERFERENCE PATTERNING
HIPSC
POLYETHYLENE TEREPHTHALATE
ROLL-TO-ROLL NANOIMPRINT LITHOGRAPHY
SURFACE TOPOGRAPHY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Surface functionalization of polymers aims to introduce novel properties that favor bioactive responses. We have investigated the possibility of surface functionalization of polyethylene terephthalate (PET) sheets by the combination of laser ablation with hot embossing and the application of such techniques in the field of stem cell research. We investigated the response of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to topography in the low micrometer range. HiPSC-CMs are expected to offer new therapeutic tools for myocardial replacement or regeneration after an infarct or other causes of cardiac tissue loss. However, hiPSC-CMs are phenotypically immature compared to myocytes in the adult myocardium, hampering their clinical application. We aimed to develop and test a high-throughput technique for surface structuring that would improve hiPSC-CMs structural maturation. We used laser ablation with a ps-laser source in combination with nanoimprint lithography to fabricate large areas of homogeneous micron- to submicron line-like pattern with a spatial period of 3 µm on the PET surface. We evaluated cell morphology, alignment, sarcomeric myofibrils assembly, and calcium transients to evaluate phenotypic changes associated with culturing hiPSC-CMs on functionalized PET. Surface functionalization through hot embossing was able to generate, at low cost, low micrometer features on the PET surface that influenced the hiPSC-CMs phenotype, suggesting improved structural and functional maturation. This technique may be relevant for high-throughput technologies that require conditioning of hiPSC-CMs and may be useful for the production of these cells for drug screening and disease modeling applications with lower costs.
Fil: Cortella, Lucas R. X.. Universidade de Sao Paulo; Brasil
Fil: Cestari, Idágene A.. Universidade de Sao Paulo; Brasil
Fil: Lahuerta, Ricardo D.. Universidade de Sao Paulo; Brasil
Fil: Arana, Matheus C.. Universidade de Sao Paulo; Brasil
Fil: Soldera, Marcos Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina
Fil: Rank, Andreas. Technische Universität Dresden; Alemania
Fil: Lasagni, Andrés F.. Technische Universität Dresden; Alemania
Fil: Cestari, Ismar N.. Universidade de Sao Paulo; Brasil
description Surface functionalization of polymers aims to introduce novel properties that favor bioactive responses. We have investigated the possibility of surface functionalization of polyethylene terephthalate (PET) sheets by the combination of laser ablation with hot embossing and the application of such techniques in the field of stem cell research. We investigated the response of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to topography in the low micrometer range. HiPSC-CMs are expected to offer new therapeutic tools for myocardial replacement or regeneration after an infarct or other causes of cardiac tissue loss. However, hiPSC-CMs are phenotypically immature compared to myocytes in the adult myocardium, hampering their clinical application. We aimed to develop and test a high-throughput technique for surface structuring that would improve hiPSC-CMs structural maturation. We used laser ablation with a ps-laser source in combination with nanoimprint lithography to fabricate large areas of homogeneous micron- to submicron line-like pattern with a spatial period of 3 µm on the PET surface. We evaluated cell morphology, alignment, sarcomeric myofibrils assembly, and calcium transients to evaluate phenotypic changes associated with culturing hiPSC-CMs on functionalized PET. Surface functionalization through hot embossing was able to generate, at low cost, low micrometer features on the PET surface that influenced the hiPSC-CMs phenotype, suggesting improved structural and functional maturation. This technique may be relevant for high-throughput technologies that require conditioning of hiPSC-CMs and may be useful for the production of these cells for drug screening and disease modeling applications with lower costs.
publishDate 2021
dc.date.none.fl_str_mv 2021-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/183417
Cortella, Lucas R. X.; Cestari, Idágene A.; Lahuerta, Ricardo D.; Arana, Matheus C.; Soldera, Marcos Maximiliano; et al.; Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology; IOP Publishing; Biomedical Materials; 16; 6; 8-2021; 1-39
1748-6041
CONICET Digital
CONICET
url http://hdl.handle.net/11336/183417
identifier_str_mv Cortella, Lucas R. X.; Cestari, Idágene A.; Lahuerta, Ricardo D.; Arana, Matheus C.; Soldera, Marcos Maximiliano; et al.; Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology; IOP Publishing; Biomedical Materials; 16; 6; 8-2021; 1-39
1748-6041
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1088/1748-605X/ac1f73
info:eu-repo/semantics/altIdentifier/doi/10.1088/1748-605X/ac1f73
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv IOP Publishing
publisher.none.fl_str_mv IOP Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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