Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation
- Autores
- Gargiulo, Ernesto; Morande, Pablo Elías; Jeyakumar, Maxmilan; Rospape, Lucie; Paggetti, Jérôme; Moussay, Etienne
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Here, we present a protocol for the direct isolation of small extracellular vesicles (sEV) from the spleen of preclinical murine models of leukemia using ultracentrifugation. We describe steps for tissue collection, sample preparation, ultracentrifugation-based isolation, and sEV characterization. This protocol allows for efficient enrichment of both leukemia and its microenvironment-derived sEV (LME-sEV), providing a valuable tool for studying their composition and functional roles. Potential applications include investigating the role of sEV in leukemia progression and identifying biomarkers.
Fil: Gargiulo, Ernesto. Luxembourg Institute of Health; Luxemburgo
Fil: Morande, Pablo Elías. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Jeyakumar, Maxmilan. Luxembourg Institute of Health; Luxemburgo
Fil: Rospape, Lucie. Luxembourg Institute of Health; Luxemburgo
Fil: Paggetti, Jérôme. Luxembourg Institute of Health; Luxemburgo
Fil: Moussay, Etienne. Luxembourg Institute of Health; Luxemburgo - Materia
-
CLL
extracellular vesicles
T cell dysfunction
TCL1 model - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/264503
Ver los metadatos del registro completo
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Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugationGargiulo, ErnestoMorande, Pablo ElíasJeyakumar, MaxmilanRospape, LuciePaggetti, JérômeMoussay, EtienneCLLextracellular vesiclesT cell dysfunctionTCL1 modelhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Here, we present a protocol for the direct isolation of small extracellular vesicles (sEV) from the spleen of preclinical murine models of leukemia using ultracentrifugation. We describe steps for tissue collection, sample preparation, ultracentrifugation-based isolation, and sEV characterization. This protocol allows for efficient enrichment of both leukemia and its microenvironment-derived sEV (LME-sEV), providing a valuable tool for studying their composition and functional roles. Potential applications include investigating the role of sEV in leukemia progression and identifying biomarkers.Fil: Gargiulo, Ernesto. Luxembourg Institute of Health; LuxemburgoFil: Morande, Pablo Elías. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Jeyakumar, Maxmilan. Luxembourg Institute of Health; LuxemburgoFil: Rospape, Lucie. Luxembourg Institute of Health; LuxemburgoFil: Paggetti, Jérôme. Luxembourg Institute of Health; LuxemburgoFil: Moussay, Etienne. Luxembourg Institute of Health; LuxemburgoCell Press2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/264503Gargiulo, Ernesto; Morande, Pablo Elías; Jeyakumar, Maxmilan; Rospape, Lucie; Paggetti, Jérôme; et al.; Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation; Cell Press; STAR Protocols; 5; 3; 10-2024; 1-132666-1667CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S266616672400409X?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.xpro.2024.103244info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:23Zoai:ri.conicet.gov.ar:11336/264503instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:24.144CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation |
title |
Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation |
spellingShingle |
Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation Gargiulo, Ernesto CLL extracellular vesicles T cell dysfunction TCL1 model |
title_short |
Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation |
title_full |
Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation |
title_fullStr |
Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation |
title_full_unstemmed |
Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation |
title_sort |
Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation |
dc.creator.none.fl_str_mv |
Gargiulo, Ernesto Morande, Pablo Elías Jeyakumar, Maxmilan Rospape, Lucie Paggetti, Jérôme Moussay, Etienne |
author |
Gargiulo, Ernesto |
author_facet |
Gargiulo, Ernesto Morande, Pablo Elías Jeyakumar, Maxmilan Rospape, Lucie Paggetti, Jérôme Moussay, Etienne |
author_role |
author |
author2 |
Morande, Pablo Elías Jeyakumar, Maxmilan Rospape, Lucie Paggetti, Jérôme Moussay, Etienne |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
CLL extracellular vesicles T cell dysfunction TCL1 model |
topic |
CLL extracellular vesicles T cell dysfunction TCL1 model |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Here, we present a protocol for the direct isolation of small extracellular vesicles (sEV) from the spleen of preclinical murine models of leukemia using ultracentrifugation. We describe steps for tissue collection, sample preparation, ultracentrifugation-based isolation, and sEV characterization. This protocol allows for efficient enrichment of both leukemia and its microenvironment-derived sEV (LME-sEV), providing a valuable tool for studying their composition and functional roles. Potential applications include investigating the role of sEV in leukemia progression and identifying biomarkers. Fil: Gargiulo, Ernesto. Luxembourg Institute of Health; Luxemburgo Fil: Morande, Pablo Elías. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Jeyakumar, Maxmilan. Luxembourg Institute of Health; Luxemburgo Fil: Rospape, Lucie. Luxembourg Institute of Health; Luxemburgo Fil: Paggetti, Jérôme. Luxembourg Institute of Health; Luxemburgo Fil: Moussay, Etienne. Luxembourg Institute of Health; Luxemburgo |
description |
Here, we present a protocol for the direct isolation of small extracellular vesicles (sEV) from the spleen of preclinical murine models of leukemia using ultracentrifugation. We describe steps for tissue collection, sample preparation, ultracentrifugation-based isolation, and sEV characterization. This protocol allows for efficient enrichment of both leukemia and its microenvironment-derived sEV (LME-sEV), providing a valuable tool for studying their composition and functional roles. Potential applications include investigating the role of sEV in leukemia progression and identifying biomarkers. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/264503 Gargiulo, Ernesto; Morande, Pablo Elías; Jeyakumar, Maxmilan; Rospape, Lucie; Paggetti, Jérôme; et al.; Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation; Cell Press; STAR Protocols; 5; 3; 10-2024; 1-13 2666-1667 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/264503 |
identifier_str_mv |
Gargiulo, Ernesto; Morande, Pablo Elías; Jeyakumar, Maxmilan; Rospape, Lucie; Paggetti, Jérôme; et al.; Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation; Cell Press; STAR Protocols; 5; 3; 10-2024; 1-13 2666-1667 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S266616672400409X?via%3Dihub info:eu-repo/semantics/altIdentifier/doi/10.1016/j.xpro.2024.103244 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Cell Press |
publisher.none.fl_str_mv |
Cell Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614431631212544 |
score |
13.070432 |