Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation

Autores
Gargiulo, Ernesto; Morande, Pablo Elías; Jeyakumar, Maxmilan; Rospape, Lucie; Paggetti, Jérôme; Moussay, Etienne
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Here, we present a protocol for the direct isolation of small extracellular vesicles (sEV) from the spleen of preclinical murine models of leukemia using ultracentrifugation. We describe steps for tissue collection, sample preparation, ultracentrifugation-based isolation, and sEV characterization. This protocol allows for efficient enrichment of both leukemia and its microenvironment-derived sEV (LME-sEV), providing a valuable tool for studying their composition and functional roles. Potential applications include investigating the role of sEV in leukemia progression and identifying biomarkers.
Fil: Gargiulo, Ernesto. Luxembourg Institute of Health; Luxemburgo
Fil: Morande, Pablo Elías. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Jeyakumar, Maxmilan. Luxembourg Institute of Health; Luxemburgo
Fil: Rospape, Lucie. Luxembourg Institute of Health; Luxemburgo
Fil: Paggetti, Jérôme. Luxembourg Institute of Health; Luxemburgo
Fil: Moussay, Etienne. Luxembourg Institute of Health; Luxemburgo
Materia
CLL
extracellular vesicles
T cell dysfunction
TCL1 model
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/264503
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/264503
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugationGargiulo, ErnestoMorande, Pablo ElíasJeyakumar, MaxmilanRospape, LuciePaggetti, JérômeMoussay, EtienneCLLextracellular vesiclesT cell dysfunctionTCL1 modelhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Here, we present a protocol for the direct isolation of small extracellular vesicles (sEV) from the spleen of preclinical murine models of leukemia using ultracentrifugation. We describe steps for tissue collection, sample preparation, ultracentrifugation-based isolation, and sEV characterization. This protocol allows for efficient enrichment of both leukemia and its microenvironment-derived sEV (LME-sEV), providing a valuable tool for studying their composition and functional roles. Potential applications include investigating the role of sEV in leukemia progression and identifying biomarkers.Fil: Gargiulo, Ernesto. Luxembourg Institute of Health; LuxemburgoFil: Morande, Pablo Elías. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Jeyakumar, Maxmilan. Luxembourg Institute of Health; LuxemburgoFil: Rospape, Lucie. Luxembourg Institute of Health; LuxemburgoFil: Paggetti, Jérôme. Luxembourg Institute of Health; LuxemburgoFil: Moussay, Etienne. Luxembourg Institute of Health; LuxemburgoCell Press2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/264503Gargiulo, Ernesto; Morande, Pablo Elías; Jeyakumar, Maxmilan; Rospape, Lucie; Paggetti, Jérôme; et al.; Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation; Cell Press; STAR Protocols; 5; 3; 10-2024; 1-132666-1667CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S266616672400409X?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.xpro.2024.103244info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:38:10Zoai:ri.conicet.gov.ar:11336/264503instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:38:10.676CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation
title Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation
spellingShingle Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation
Gargiulo, Ernesto
CLL
extracellular vesicles
T cell dysfunction
TCL1 model
title_short Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation
title_full Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation
title_fullStr Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation
title_full_unstemmed Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation
title_sort Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation
dc.creator.none.fl_str_mv Gargiulo, Ernesto
Morande, Pablo Elías
Jeyakumar, Maxmilan
Rospape, Lucie
Paggetti, Jérôme
Moussay, Etienne
author Gargiulo, Ernesto
author_facet Gargiulo, Ernesto
Morande, Pablo Elías
Jeyakumar, Maxmilan
Rospape, Lucie
Paggetti, Jérôme
Moussay, Etienne
author_role author
author2 Morande, Pablo Elías
Jeyakumar, Maxmilan
Rospape, Lucie
Paggetti, Jérôme
Moussay, Etienne
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CLL
extracellular vesicles
T cell dysfunction
TCL1 model
topic CLL
extracellular vesicles
T cell dysfunction
TCL1 model
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Here, we present a protocol for the direct isolation of small extracellular vesicles (sEV) from the spleen of preclinical murine models of leukemia using ultracentrifugation. We describe steps for tissue collection, sample preparation, ultracentrifugation-based isolation, and sEV characterization. This protocol allows for efficient enrichment of both leukemia and its microenvironment-derived sEV (LME-sEV), providing a valuable tool for studying their composition and functional roles. Potential applications include investigating the role of sEV in leukemia progression and identifying biomarkers.
Fil: Gargiulo, Ernesto. Luxembourg Institute of Health; Luxemburgo
Fil: Morande, Pablo Elías. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Jeyakumar, Maxmilan. Luxembourg Institute of Health; Luxemburgo
Fil: Rospape, Lucie. Luxembourg Institute of Health; Luxemburgo
Fil: Paggetti, Jérôme. Luxembourg Institute of Health; Luxemburgo
Fil: Moussay, Etienne. Luxembourg Institute of Health; Luxemburgo
description Here, we present a protocol for the direct isolation of small extracellular vesicles (sEV) from the spleen of preclinical murine models of leukemia using ultracentrifugation. We describe steps for tissue collection, sample preparation, ultracentrifugation-based isolation, and sEV characterization. This protocol allows for efficient enrichment of both leukemia and its microenvironment-derived sEV (LME-sEV), providing a valuable tool for studying their composition and functional roles. Potential applications include investigating the role of sEV in leukemia progression and identifying biomarkers.
publishDate 2024
dc.date.none.fl_str_mv 2024-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/264503
Gargiulo, Ernesto; Morande, Pablo Elías; Jeyakumar, Maxmilan; Rospape, Lucie; Paggetti, Jérôme; et al.; Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation; Cell Press; STAR Protocols; 5; 3; 10-2024; 1-13
2666-1667
CONICET Digital
CONICET
url http://hdl.handle.net/11336/264503
identifier_str_mv Gargiulo, Ernesto; Morande, Pablo Elías; Jeyakumar, Maxmilan; Rospape, Lucie; Paggetti, Jérôme; et al.; Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation; Cell Press; STAR Protocols; 5; 3; 10-2024; 1-13
2666-1667
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S266616672400409X?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.xpro.2024.103244
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083500978995200
score 13.22299

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