First characterization of major histocompatibility complex class II DRB3 diversity in cattle breeds raised in Egypt

Autores
Hamada, Rania; Giovambattista, Guillermo; Metwally, Samy; Borjigin, Liushiqi; Polat Yamanaka, Meripet; Matsuura, Ryosuke; Ali, Alsagher O.; Mahmoud, Hassan Y. A. H.; Mohamed, Adel E. A.; Kyaw Moe, Kyaw; Takeshima, Shin Nosuke; Wada, Satoshi; Aida, Yoko
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Genes encoding bovine leukocyte antigen (BoLA) enable the immune system to identify pathogens. Therefore, these genes have been used as genetic markers for infectious and autoimmune diseases as well as for immunological traits in cattle. Although BoLA polymorphisms have been reported in various cattle breeds worldwide, they have not been studied in cattle populations in Egypt. In this study, we characterized BoLA-DRB3 in two local Egyptian populations and one foreign population using polymerase chain reaction-sequence-based typing (PCRSBT) method. Fifty-four previously reported BoLA-DRB3 alleles and eight new alleles (BoLA-DRB3*005:08, *015:07, *016:03, *017:04, *020:02:02, *021:03, *164:01, and *165:01) were identified. Alignment analysis of the eight new alleles revealed 90.7–98.9 %, and 83.1–97.8 % nucleotide and amino acid identities, respectively, with the BoLA-DRB3 cDNA clone NR-1. Interestingly, BoLA-DRB3 in Egyptian cattle showed a high degree of allelic diversity in native (na = 28, hE > 0.95), mixed (na = 61, hE > 0.96), and Holstein (na = 18, hE > 0.88) populations. BoLA-DRB3*002:01 (14.3 %), BoLA-DRB3*001:01 (8.5 %), and BoLA-DRB3*015:01 (20.2 %) were the most frequent alleles in native, mixed, and Holstein populations, respectively, indicating that the genetic profiles differed in each population. Based on the allele frequencies of BoLA-DRB3, genetic variation among Egyptian, Asian, African, and American breeds was examined using Nei’s distances and principal component analysis. The results suggested that native and mixed cattle populations were most closely associated with African breeds in terms of their gene pool, whereas Holstein cattle were more distinct from the other breeds and were closely related to Holstein cattle populations from other countries.
Fil: Hamada, Rania. Viral Infectious Diseases Unit; Japón. Damanhour University; Egipto
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. University of Tokyo; Japón
Fil: Metwally, Samy. Viral Infectious Diseases Unit; Japón. University of Tokyo; Japón
Fil: Borjigin, Liushiqi. Viral Infectious Diseases Unit; Japón
Fil: Polat Yamanaka, Meripet. Viral Infectious Diseases Unit; Japón. University of Tokyo; Japón
Fil: Matsuura, Ryosuke. Viral Infectious Diseases Unit; Japón. University of Tokyo; Japón
Fil: Ali, Alsagher O.. South Valley University; Egipto
Fil: Mahmoud, Hassan Y. A. H.. South Valley University; Egipto
Fil: Mohamed, Adel E. A.. South Valley University; Egipto
Fil: Kyaw Moe, Kyaw. Viral Infectious Diseases Unit; Japón. University of Veterinary Science; Birmania
Fil: Takeshima, Shin Nosuke. Jumonji University; Japón
Fil: Wada, Satoshi. Viral Infectious Diseases Unit; Japón
Fil: Aida, Yoko. Viral Infectious Diseases Unit; Japón. University of Tokyo; Japón
Materia
Allele frequency
BoLA-DRB3 polymorphism
Cattle populations in Egypt
PCR-Sequence-based typing
Population tree
Principal component analysis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/266648
Acceder
id CONICETDig_c616b7a28a30e13b86b4bfb29f3c00fe
oai_identifier_str oai:ri.conicet.gov.ar:11336/266648
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First characterization of major histocompatibility complex class II DRB3 diversity in cattle breeds raised in EgyptHamada, RaniaGiovambattista, GuillermoMetwally, SamyBorjigin, LiushiqiPolat Yamanaka, MeripetMatsuura, RyosukeAli, Alsagher O.Mahmoud, Hassan Y. A. H.Mohamed, Adel E. A.Kyaw Moe, KyawTakeshima, Shin NosukeWada, SatoshiAida, YokoAllele frequencyBoLA-DRB3 polymorphismCattle populations in EgyptPCR-Sequence-based typingPopulation treePrincipal component analysishttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Genes encoding bovine leukocyte antigen (BoLA) enable the immune system to identify pathogens. Therefore, these genes have been used as genetic markers for infectious and autoimmune diseases as well as for immunological traits in cattle. Although BoLA polymorphisms have been reported in various cattle breeds worldwide, they have not been studied in cattle populations in Egypt. In this study, we characterized BoLA-DRB3 in two local Egyptian populations and one foreign population using polymerase chain reaction-sequence-based typing (PCRSBT) method. Fifty-four previously reported BoLA-DRB3 alleles and eight new alleles (BoLA-DRB3*005:08, *015:07, *016:03, *017:04, *020:02:02, *021:03, *164:01, and *165:01) were identified. Alignment analysis of the eight new alleles revealed 90.7–98.9 %, and 83.1–97.8 % nucleotide and amino acid identities, respectively, with the BoLA-DRB3 cDNA clone NR-1. Interestingly, BoLA-DRB3 in Egyptian cattle showed a high degree of allelic diversity in native (na = 28, hE > 0.95), mixed (na = 61, hE > 0.96), and Holstein (na = 18, hE > 0.88) populations. BoLA-DRB3*002:01 (14.3 %), BoLA-DRB3*001:01 (8.5 %), and BoLA-DRB3*015:01 (20.2 %) were the most frequent alleles in native, mixed, and Holstein populations, respectively, indicating that the genetic profiles differed in each population. Based on the allele frequencies of BoLA-DRB3, genetic variation among Egyptian, Asian, African, and American breeds was examined using Nei’s distances and principal component analysis. The results suggested that native and mixed cattle populations were most closely associated with African breeds in terms of their gene pool, whereas Holstein cattle were more distinct from the other breeds and were closely related to Holstein cattle populations from other countries.Fil: Hamada, Rania. Viral Infectious Diseases Unit; Japón. Damanhour University; EgiptoFil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. University of Tokyo; JapónFil: Metwally, Samy. Viral Infectious Diseases Unit; Japón. University of Tokyo; JapónFil: Borjigin, Liushiqi. Viral Infectious Diseases Unit; JapónFil: Polat Yamanaka, Meripet. Viral Infectious Diseases Unit; Japón. University of Tokyo; JapónFil: Matsuura, Ryosuke. Viral Infectious Diseases Unit; Japón. University of Tokyo; JapónFil: Ali, Alsagher O.. South Valley University; EgiptoFil: Mahmoud, Hassan Y. A. H.. South Valley University; EgiptoFil: Mohamed, Adel E. A.. South Valley University; EgiptoFil: Kyaw Moe, Kyaw. Viral Infectious Diseases Unit; Japón. University of Veterinary Science; BirmaniaFil: Takeshima, Shin Nosuke. Jumonji University; JapónFil: Wada, Satoshi. Viral Infectious Diseases Unit; JapónFil: Aida, Yoko. Viral Infectious Diseases Unit; Japón. University of Tokyo; JapónElsevier Science2024-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/266648Hamada, Rania; Giovambattista, Guillermo; Metwally, Samy; Borjigin, Liushiqi; Polat Yamanaka, Meripet; et al.; First characterization of major histocompatibility complex class II DRB3 diversity in cattle breeds raised in Egypt; Elsevier Science; Gene; 918; 148491; 8-2024; 1-110378-1119CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.gene.2024.148491info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S037811192400372Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:46Zoai:ri.conicet.gov.ar:11336/266648instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:46.365CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First characterization of major histocompatibility complex class II DRB3 diversity in cattle breeds raised in Egypt
title First characterization of major histocompatibility complex class II DRB3 diversity in cattle breeds raised in Egypt
spellingShingle First characterization of major histocompatibility complex class II DRB3 diversity in cattle breeds raised in Egypt
Hamada, Rania
Allele frequency
BoLA-DRB3 polymorphism
Cattle populations in Egypt
PCR-Sequence-based typing
Population tree
Principal component analysis
title_short First characterization of major histocompatibility complex class II DRB3 diversity in cattle breeds raised in Egypt
title_full First characterization of major histocompatibility complex class II DRB3 diversity in cattle breeds raised in Egypt
title_fullStr First characterization of major histocompatibility complex class II DRB3 diversity in cattle breeds raised in Egypt
title_full_unstemmed First characterization of major histocompatibility complex class II DRB3 diversity in cattle breeds raised in Egypt
title_sort First characterization of major histocompatibility complex class II DRB3 diversity in cattle breeds raised in Egypt
dc.creator.none.fl_str_mv Hamada, Rania
Giovambattista, Guillermo
Metwally, Samy
Borjigin, Liushiqi
Polat Yamanaka, Meripet
Matsuura, Ryosuke
Ali, Alsagher O.
Mahmoud, Hassan Y. A. H.
Mohamed, Adel E. A.
Kyaw Moe, Kyaw
Takeshima, Shin Nosuke
Wada, Satoshi
Aida, Yoko
author Hamada, Rania
author_facet Hamada, Rania
Giovambattista, Guillermo
Metwally, Samy
Borjigin, Liushiqi
Polat Yamanaka, Meripet
Matsuura, Ryosuke
Ali, Alsagher O.
Mahmoud, Hassan Y. A. H.
Mohamed, Adel E. A.
Kyaw Moe, Kyaw
Takeshima, Shin Nosuke
Wada, Satoshi
Aida, Yoko
author_role author
author2 Giovambattista, Guillermo
Metwally, Samy
Borjigin, Liushiqi
Polat Yamanaka, Meripet
Matsuura, Ryosuke
Ali, Alsagher O.
Mahmoud, Hassan Y. A. H.
Mohamed, Adel E. A.
Kyaw Moe, Kyaw
Takeshima, Shin Nosuke
Wada, Satoshi
Aida, Yoko
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Allele frequency
BoLA-DRB3 polymorphism
Cattle populations in Egypt
PCR-Sequence-based typing
Population tree
Principal component analysis
topic Allele frequency
BoLA-DRB3 polymorphism
Cattle populations in Egypt
PCR-Sequence-based typing
Population tree
Principal component analysis
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Genes encoding bovine leukocyte antigen (BoLA) enable the immune system to identify pathogens. Therefore, these genes have been used as genetic markers for infectious and autoimmune diseases as well as for immunological traits in cattle. Although BoLA polymorphisms have been reported in various cattle breeds worldwide, they have not been studied in cattle populations in Egypt. In this study, we characterized BoLA-DRB3 in two local Egyptian populations and one foreign population using polymerase chain reaction-sequence-based typing (PCRSBT) method. Fifty-four previously reported BoLA-DRB3 alleles and eight new alleles (BoLA-DRB3*005:08, *015:07, *016:03, *017:04, *020:02:02, *021:03, *164:01, and *165:01) were identified. Alignment analysis of the eight new alleles revealed 90.7–98.9 %, and 83.1–97.8 % nucleotide and amino acid identities, respectively, with the BoLA-DRB3 cDNA clone NR-1. Interestingly, BoLA-DRB3 in Egyptian cattle showed a high degree of allelic diversity in native (na = 28, hE > 0.95), mixed (na = 61, hE > 0.96), and Holstein (na = 18, hE > 0.88) populations. BoLA-DRB3*002:01 (14.3 %), BoLA-DRB3*001:01 (8.5 %), and BoLA-DRB3*015:01 (20.2 %) were the most frequent alleles in native, mixed, and Holstein populations, respectively, indicating that the genetic profiles differed in each population. Based on the allele frequencies of BoLA-DRB3, genetic variation among Egyptian, Asian, African, and American breeds was examined using Nei’s distances and principal component analysis. The results suggested that native and mixed cattle populations were most closely associated with African breeds in terms of their gene pool, whereas Holstein cattle were more distinct from the other breeds and were closely related to Holstein cattle populations from other countries.
Fil: Hamada, Rania. Viral Infectious Diseases Unit; Japón. Damanhour University; Egipto
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. University of Tokyo; Japón
Fil: Metwally, Samy. Viral Infectious Diseases Unit; Japón. University of Tokyo; Japón
Fil: Borjigin, Liushiqi. Viral Infectious Diseases Unit; Japón
Fil: Polat Yamanaka, Meripet. Viral Infectious Diseases Unit; Japón. University of Tokyo; Japón
Fil: Matsuura, Ryosuke. Viral Infectious Diseases Unit; Japón. University of Tokyo; Japón
Fil: Ali, Alsagher O.. South Valley University; Egipto
Fil: Mahmoud, Hassan Y. A. H.. South Valley University; Egipto
Fil: Mohamed, Adel E. A.. South Valley University; Egipto
Fil: Kyaw Moe, Kyaw. Viral Infectious Diseases Unit; Japón. University of Veterinary Science; Birmania
Fil: Takeshima, Shin Nosuke. Jumonji University; Japón
Fil: Wada, Satoshi. Viral Infectious Diseases Unit; Japón
Fil: Aida, Yoko. Viral Infectious Diseases Unit; Japón. University of Tokyo; Japón
description Genes encoding bovine leukocyte antigen (BoLA) enable the immune system to identify pathogens. Therefore, these genes have been used as genetic markers for infectious and autoimmune diseases as well as for immunological traits in cattle. Although BoLA polymorphisms have been reported in various cattle breeds worldwide, they have not been studied in cattle populations in Egypt. In this study, we characterized BoLA-DRB3 in two local Egyptian populations and one foreign population using polymerase chain reaction-sequence-based typing (PCRSBT) method. Fifty-four previously reported BoLA-DRB3 alleles and eight new alleles (BoLA-DRB3*005:08, *015:07, *016:03, *017:04, *020:02:02, *021:03, *164:01, and *165:01) were identified. Alignment analysis of the eight new alleles revealed 90.7–98.9 %, and 83.1–97.8 % nucleotide and amino acid identities, respectively, with the BoLA-DRB3 cDNA clone NR-1. Interestingly, BoLA-DRB3 in Egyptian cattle showed a high degree of allelic diversity in native (na = 28, hE > 0.95), mixed (na = 61, hE > 0.96), and Holstein (na = 18, hE > 0.88) populations. BoLA-DRB3*002:01 (14.3 %), BoLA-DRB3*001:01 (8.5 %), and BoLA-DRB3*015:01 (20.2 %) were the most frequent alleles in native, mixed, and Holstein populations, respectively, indicating that the genetic profiles differed in each population. Based on the allele frequencies of BoLA-DRB3, genetic variation among Egyptian, Asian, African, and American breeds was examined using Nei’s distances and principal component analysis. The results suggested that native and mixed cattle populations were most closely associated with African breeds in terms of their gene pool, whereas Holstein cattle were more distinct from the other breeds and were closely related to Holstein cattle populations from other countries.
publishDate 2024
dc.date.none.fl_str_mv 2024-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/266648
Hamada, Rania; Giovambattista, Guillermo; Metwally, Samy; Borjigin, Liushiqi; Polat Yamanaka, Meripet; et al.; First characterization of major histocompatibility complex class II DRB3 diversity in cattle breeds raised in Egypt; Elsevier Science; Gene; 918; 148491; 8-2024; 1-11
0378-1119
CONICET Digital
CONICET
url http://hdl.handle.net/11336/266648
identifier_str_mv Hamada, Rania; Giovambattista, Guillermo; Metwally, Samy; Borjigin, Liushiqi; Polat Yamanaka, Meripet; et al.; First characterization of major histocompatibility complex class II DRB3 diversity in cattle breeds raised in Egypt; Elsevier Science; Gene; 918; 148491; 8-2024; 1-11
0378-1119
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.gene.2024.148491
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S037811192400372X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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