Using fluorescent lipids contributes to the active learning of principles underlying lipid signaling

Autores
Peppino Margutti, Micaela Yesica; Reyna, Mercedes; Villasuso, Ana Laura
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The concepts of phospholipase activity is often taught in undergraduate biology and biochemistry classes and reinforced in laboratory exercises. However, very rarely does the design of these exercises allow students to directly gain experience in the use of modern instruments such as digital imaging systems and fluorescence spectrophotometers. The laboratory exercise described here involves the use of fluorescent lipids to evaluate phospholipase activity. Students use thin layer chromatography (TLC) to understand how lipids change under different conditions (i.e. abiotic and biotic stress). They explore strategies to separate, visualize and quantify lipids by TLC, digital imaging, and fluorometry. They also have increased opportunities for hands‐on practise with experimental design, liposome sample preparation, and implementation of instrumentation commonly used by experienced researchers; all while learning and applying fundamental concepts about lipids.
Fil: Peppino Margutti, Micaela Yesica. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Reyna, Mercedes. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Villasuso, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
FLUORESCENT LIPIDS
PHOSPHOLIPASE A
PHOSPHOLIPASE D
THIN LAYER CHROMATOGRAPHY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/124538
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Using fluorescent lipids contributes to the active learning of principles underlying lipid signalingPeppino Margutti, Micaela YesicaReyna, MercedesVillasuso, Ana LauraFLUORESCENT LIPIDSPHOSPHOLIPASE APHOSPHOLIPASE DTHIN LAYER CHROMATOGRAPHYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The concepts of phospholipase activity is often taught in undergraduate biology and biochemistry classes and reinforced in laboratory exercises. However, very rarely does the design of these exercises allow students to directly gain experience in the use of modern instruments such as digital imaging systems and fluorescence spectrophotometers. The laboratory exercise described here involves the use of fluorescent lipids to evaluate phospholipase activity. Students use thin layer chromatography (TLC) to understand how lipids change under different conditions (i.e. abiotic and biotic stress). They explore strategies to separate, visualize and quantify lipids by TLC, digital imaging, and fluorometry. They also have increased opportunities for hands‐on practise with experimental design, liposome sample preparation, and implementation of instrumentation commonly used by experienced researchers; all while learning and applying fundamental concepts about lipids.Fil: Peppino Margutti, Micaela Yesica. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Reyna, Mercedes. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Villasuso, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaJohn Wiley & Sons Inc2018-12-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/124538Peppino Margutti, Micaela Yesica; Reyna, Mercedes; Villasuso, Ana Laura; Using fluorescent lipids contributes to the active learning of principles underlying lipid signaling; John Wiley & Sons Inc; Biochemistry and Molecular Biology Education; 47; 1; 25-12-2018; 100-1051470-81751539-3429CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/bmb.21201info:eu-repo/semantics/altIdentifier/url/https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/bmb.21201info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:39:44Zoai:ri.conicet.gov.ar:11336/124538instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:39:45.228CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Using fluorescent lipids contributes to the active learning of principles underlying lipid signaling
title Using fluorescent lipids contributes to the active learning of principles underlying lipid signaling
spellingShingle Using fluorescent lipids contributes to the active learning of principles underlying lipid signaling
Peppino Margutti, Micaela Yesica
FLUORESCENT LIPIDS
PHOSPHOLIPASE A
PHOSPHOLIPASE D
THIN LAYER CHROMATOGRAPHY
title_short Using fluorescent lipids contributes to the active learning of principles underlying lipid signaling
title_full Using fluorescent lipids contributes to the active learning of principles underlying lipid signaling
title_fullStr Using fluorescent lipids contributes to the active learning of principles underlying lipid signaling
title_full_unstemmed Using fluorescent lipids contributes to the active learning of principles underlying lipid signaling
title_sort Using fluorescent lipids contributes to the active learning of principles underlying lipid signaling
dc.creator.none.fl_str_mv Peppino Margutti, Micaela Yesica
Reyna, Mercedes
Villasuso, Ana Laura
author Peppino Margutti, Micaela Yesica
author_facet Peppino Margutti, Micaela Yesica
Reyna, Mercedes
Villasuso, Ana Laura
author_role author
author2 Reyna, Mercedes
Villasuso, Ana Laura
author2_role author
author
dc.subject.none.fl_str_mv FLUORESCENT LIPIDS
PHOSPHOLIPASE A
PHOSPHOLIPASE D
THIN LAYER CHROMATOGRAPHY
topic FLUORESCENT LIPIDS
PHOSPHOLIPASE A
PHOSPHOLIPASE D
THIN LAYER CHROMATOGRAPHY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The concepts of phospholipase activity is often taught in undergraduate biology and biochemistry classes and reinforced in laboratory exercises. However, very rarely does the design of these exercises allow students to directly gain experience in the use of modern instruments such as digital imaging systems and fluorescence spectrophotometers. The laboratory exercise described here involves the use of fluorescent lipids to evaluate phospholipase activity. Students use thin layer chromatography (TLC) to understand how lipids change under different conditions (i.e. abiotic and biotic stress). They explore strategies to separate, visualize and quantify lipids by TLC, digital imaging, and fluorometry. They also have increased opportunities for hands‐on practise with experimental design, liposome sample preparation, and implementation of instrumentation commonly used by experienced researchers; all while learning and applying fundamental concepts about lipids.
Fil: Peppino Margutti, Micaela Yesica. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Reyna, Mercedes. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Villasuso, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description The concepts of phospholipase activity is often taught in undergraduate biology and biochemistry classes and reinforced in laboratory exercises. However, very rarely does the design of these exercises allow students to directly gain experience in the use of modern instruments such as digital imaging systems and fluorescence spectrophotometers. The laboratory exercise described here involves the use of fluorescent lipids to evaluate phospholipase activity. Students use thin layer chromatography (TLC) to understand how lipids change under different conditions (i.e. abiotic and biotic stress). They explore strategies to separate, visualize and quantify lipids by TLC, digital imaging, and fluorometry. They also have increased opportunities for hands‐on practise with experimental design, liposome sample preparation, and implementation of instrumentation commonly used by experienced researchers; all while learning and applying fundamental concepts about lipids.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-25
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/124538
Peppino Margutti, Micaela Yesica; Reyna, Mercedes; Villasuso, Ana Laura; Using fluorescent lipids contributes to the active learning of principles underlying lipid signaling; John Wiley & Sons Inc; Biochemistry and Molecular Biology Education; 47; 1; 25-12-2018; 100-105
1470-8175
1539-3429
CONICET Digital
CONICET
url http://hdl.handle.net/11336/124538
identifier_str_mv Peppino Margutti, Micaela Yesica; Reyna, Mercedes; Villasuso, Ana Laura; Using fluorescent lipids contributes to the active learning of principles underlying lipid signaling; John Wiley & Sons Inc; Biochemistry and Molecular Biology Education; 47; 1; 25-12-2018; 100-105
1470-8175
1539-3429
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/bmb.21201
info:eu-repo/semantics/altIdentifier/url/https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/bmb.21201
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Inc
publisher.none.fl_str_mv John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.891075

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