Easy and Fast Preparation of Large and Giant Vesicles from Highly Confined Thin Lipid Films Deposited at the Air–Water Interface

Autores
Bavastrello, Valter; Caliari, Adriano; Pesce, Isabella; Bagatolli, Luis Alberto; Hanczyc, Martin M.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lipid vesicles are supramolecular structures of great interest for industrial and research applications. They can be used simply to compartmentalize solutions and pack active molecules in femtoliter-scale volumes or as highly sophisticated drug delivery vehicles and dynamic cell-size bioreactors. For these reasons, many methods for the production of vesicles have been developed, and some of them present several drawbacks, such as long working times and the requirement of specific equipment to perform the technique. In this work, we present a method to produce vesicles from highly confined lipid films at the air–water interface. The procedure involves two simple steps: the formation of the thin lipid film at the air–water interface and then brief sonication (10 s). These films are obtained by depositing different aliquots of lipid organic solutions at the air–liquid interface of round-bottom Eppendorfs tubes. The morphology of the highly confined lipid thin films was studied by optical microscopy noting the formation of non-uniform depositions at the air–liquid interface, with the presence of thicker portions close to the container sidewall. Post-sonication, the presence of vesicles composed of 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC) or 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) was confirmed using the complementary techniques of fluorescence microscopy and flow cytometry. The size distribution investigations carried out by flow cytometry revealed the optimal concentrations to favor the formation of giant vesicles (GVs). Furthermore, we investigated aqueous phase encapsulation by adding calcein or green fluorescent protein (GFP) to the aqueous phase then characterized by fluorescence microscopy and flow cytometry. We demonstrate a fast and easy method for producing vesicles including GVs on demand.
Fil: Bavastrello, Valter. Universidad de Trento; Italia
Fil: Caliari, Adriano. Universidad de Trento; Italia
Fil: Pesce, Isabella. Universidad de Trento; Italia
Fil: Bagatolli, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of New Mexico; Estados Unidos
Fil: Hanczyc, Martin M.. Universidad de Trento; Italia. Yachay EP and Yachay Tech University; Ecuador
Materia
Encapsulation
Flow Cytometry
Giant Vesicles
Langmuir Films
Microscopy
Phospholipids
Protocol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/63939
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/63939
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Easy and Fast Preparation of Large and Giant Vesicles from Highly Confined Thin Lipid Films Deposited at the Air–Water InterfaceBavastrello, ValterCaliari, AdrianoPesce, IsabellaBagatolli, Luis AlbertoHanczyc, Martin M.EncapsulationFlow CytometryGiant VesiclesLangmuir FilmsMicroscopyPhospholipidsProtocolhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lipid vesicles are supramolecular structures of great interest for industrial and research applications. They can be used simply to compartmentalize solutions and pack active molecules in femtoliter-scale volumes or as highly sophisticated drug delivery vehicles and dynamic cell-size bioreactors. For these reasons, many methods for the production of vesicles have been developed, and some of them present several drawbacks, such as long working times and the requirement of specific equipment to perform the technique. In this work, we present a method to produce vesicles from highly confined lipid films at the air–water interface. The procedure involves two simple steps: the formation of the thin lipid film at the air–water interface and then brief sonication (10 s). These films are obtained by depositing different aliquots of lipid organic solutions at the air–liquid interface of round-bottom Eppendorfs tubes. The morphology of the highly confined lipid thin films was studied by optical microscopy noting the formation of non-uniform depositions at the air–liquid interface, with the presence of thicker portions close to the container sidewall. Post-sonication, the presence of vesicles composed of 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC) or 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) was confirmed using the complementary techniques of fluorescence microscopy and flow cytometry. The size distribution investigations carried out by flow cytometry revealed the optimal concentrations to favor the formation of giant vesicles (GVs). Furthermore, we investigated aqueous phase encapsulation by adding calcein or green fluorescent protein (GFP) to the aqueous phase then characterized by fluorescence microscopy and flow cytometry. We demonstrate a fast and easy method for producing vesicles including GVs on demand.Fil: Bavastrello, Valter. Universidad de Trento; ItaliaFil: Caliari, Adriano. Universidad de Trento; ItaliaFil: Pesce, Isabella. Universidad de Trento; ItaliaFil: Bagatolli, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of New Mexico; Estados UnidosFil: Hanczyc, Martin M.. Universidad de Trento; Italia. Yachay EP and Yachay Tech University; EcuadorSpringer New York LLC2018-03-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/63939Bavastrello, Valter; Caliari, Adriano; Pesce, Isabella; Bagatolli, Luis Alberto; Hanczyc, Martin M.; Easy and Fast Preparation of Large and Giant Vesicles from Highly Confined Thin Lipid Films Deposited at the Air–Water Interface; Springer New York LLC; BioNanoScience; 8; 1; 31-3-2018; 207-2172191-16302191-1649CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s12668-017-0464-5info:eu-repo/semantics/altIdentifier/doi/10.1007/s12668-017-0464-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:24Zoai:ri.conicet.gov.ar:11336/63939instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:24.768CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Easy and Fast Preparation of Large and Giant Vesicles from Highly Confined Thin Lipid Films Deposited at the Air–Water Interface
title Easy and Fast Preparation of Large and Giant Vesicles from Highly Confined Thin Lipid Films Deposited at the Air–Water Interface
spellingShingle Easy and Fast Preparation of Large and Giant Vesicles from Highly Confined Thin Lipid Films Deposited at the Air–Water Interface
Bavastrello, Valter
Encapsulation
Flow Cytometry
Giant Vesicles
Langmuir Films
Microscopy
Phospholipids
Protocol
title_short Easy and Fast Preparation of Large and Giant Vesicles from Highly Confined Thin Lipid Films Deposited at the Air–Water Interface
title_full Easy and Fast Preparation of Large and Giant Vesicles from Highly Confined Thin Lipid Films Deposited at the Air–Water Interface
title_fullStr Easy and Fast Preparation of Large and Giant Vesicles from Highly Confined Thin Lipid Films Deposited at the Air–Water Interface
title_full_unstemmed Easy and Fast Preparation of Large and Giant Vesicles from Highly Confined Thin Lipid Films Deposited at the Air–Water Interface
title_sort Easy and Fast Preparation of Large and Giant Vesicles from Highly Confined Thin Lipid Films Deposited at the Air–Water Interface
dc.creator.none.fl_str_mv Bavastrello, Valter
Caliari, Adriano
Pesce, Isabella
Bagatolli, Luis Alberto
Hanczyc, Martin M.
author Bavastrello, Valter
author_facet Bavastrello, Valter
Caliari, Adriano
Pesce, Isabella
Bagatolli, Luis Alberto
Hanczyc, Martin M.
author_role author
author2 Caliari, Adriano
Pesce, Isabella
Bagatolli, Luis Alberto
Hanczyc, Martin M.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Encapsulation
Flow Cytometry
Giant Vesicles
Langmuir Films
Microscopy
Phospholipids
Protocol
topic Encapsulation
Flow Cytometry
Giant Vesicles
Langmuir Films
Microscopy
Phospholipids
Protocol
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lipid vesicles are supramolecular structures of great interest for industrial and research applications. They can be used simply to compartmentalize solutions and pack active molecules in femtoliter-scale volumes or as highly sophisticated drug delivery vehicles and dynamic cell-size bioreactors. For these reasons, many methods for the production of vesicles have been developed, and some of them present several drawbacks, such as long working times and the requirement of specific equipment to perform the technique. In this work, we present a method to produce vesicles from highly confined lipid films at the air–water interface. The procedure involves two simple steps: the formation of the thin lipid film at the air–water interface and then brief sonication (10 s). These films are obtained by depositing different aliquots of lipid organic solutions at the air–liquid interface of round-bottom Eppendorfs tubes. The morphology of the highly confined lipid thin films was studied by optical microscopy noting the formation of non-uniform depositions at the air–liquid interface, with the presence of thicker portions close to the container sidewall. Post-sonication, the presence of vesicles composed of 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC) or 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) was confirmed using the complementary techniques of fluorescence microscopy and flow cytometry. The size distribution investigations carried out by flow cytometry revealed the optimal concentrations to favor the formation of giant vesicles (GVs). Furthermore, we investigated aqueous phase encapsulation by adding calcein or green fluorescent protein (GFP) to the aqueous phase then characterized by fluorescence microscopy and flow cytometry. We demonstrate a fast and easy method for producing vesicles including GVs on demand.
Fil: Bavastrello, Valter. Universidad de Trento; Italia
Fil: Caliari, Adriano. Universidad de Trento; Italia
Fil: Pesce, Isabella. Universidad de Trento; Italia
Fil: Bagatolli, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of New Mexico; Estados Unidos
Fil: Hanczyc, Martin M.. Universidad de Trento; Italia. Yachay EP and Yachay Tech University; Ecuador
description Lipid vesicles are supramolecular structures of great interest for industrial and research applications. They can be used simply to compartmentalize solutions and pack active molecules in femtoliter-scale volumes or as highly sophisticated drug delivery vehicles and dynamic cell-size bioreactors. For these reasons, many methods for the production of vesicles have been developed, and some of them present several drawbacks, such as long working times and the requirement of specific equipment to perform the technique. In this work, we present a method to produce vesicles from highly confined lipid films at the air–water interface. The procedure involves two simple steps: the formation of the thin lipid film at the air–water interface and then brief sonication (10 s). These films are obtained by depositing different aliquots of lipid organic solutions at the air–liquid interface of round-bottom Eppendorfs tubes. The morphology of the highly confined lipid thin films was studied by optical microscopy noting the formation of non-uniform depositions at the air–liquid interface, with the presence of thicker portions close to the container sidewall. Post-sonication, the presence of vesicles composed of 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC) or 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) was confirmed using the complementary techniques of fluorescence microscopy and flow cytometry. The size distribution investigations carried out by flow cytometry revealed the optimal concentrations to favor the formation of giant vesicles (GVs). Furthermore, we investigated aqueous phase encapsulation by adding calcein or green fluorescent protein (GFP) to the aqueous phase then characterized by fluorescence microscopy and flow cytometry. We demonstrate a fast and easy method for producing vesicles including GVs on demand.
publishDate 2018
dc.date.none.fl_str_mv 2018-03-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/63939
Bavastrello, Valter; Caliari, Adriano; Pesce, Isabella; Bagatolli, Luis Alberto; Hanczyc, Martin M.; Easy and Fast Preparation of Large and Giant Vesicles from Highly Confined Thin Lipid Films Deposited at the Air–Water Interface; Springer New York LLC; BioNanoScience; 8; 1; 31-3-2018; 207-217
2191-1630
2191-1649
CONICET Digital
CONICET
url http://hdl.handle.net/11336/63939
identifier_str_mv Bavastrello, Valter; Caliari, Adriano; Pesce, Isabella; Bagatolli, Luis Alberto; Hanczyc, Martin M.; Easy and Fast Preparation of Large and Giant Vesicles from Highly Confined Thin Lipid Films Deposited at the Air–Water Interface; Springer New York LLC; BioNanoScience; 8; 1; 31-3-2018; 207-217
2191-1630
2191-1649
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s12668-017-0464-5
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12668-017-0464-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer New York LLC
publisher.none.fl_str_mv Springer New York LLC
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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