Requirement for BDNF in the reconsolidation of fear extinction

Autores
Radiske, Andressa; Rossato, Janine Inez; Köhler, Cristiano A.; Gonzalez, Maria Carolina; Medina, Jorge Horacio; Cammarota, Martin Pablo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Therapies based on the impairment of reconsolidation or the enhancement of extinction offer the possibility of decreasing the persistent recollection of distressing memories. However, the direct interplay between reconsolidation and extinction has rarely been considered. Previously, we reported that reactivation induces reconsolidation of fear extinction memory. Here, using a step-down inhibitory avoidance learning paradigm in rats, we show that intrahippocampus infusion of function-blocking anti-BDNF antibody immediately or 6 h after extinction memory reactivation impairs the reconsolidation of extinction. Extinction memory reactivation increases proBDNF, BDNF, and tropomyosin receptor kinase B (TrkB) phosphorylation levels in dorsal CA1, while blocking BDNF maturation in the hippocampus with plasminogen activator inhibitor 1 hinders the persistence of extinction and induces the recurrence of fear. Moreover, coinfusion of recombinant BDNF (0.25 μg/side) after extinction memory reactivation impedes the recovery of the avoidance response induced by inhibiting gene expression and protein synthesis in the dorsal hippocampus. Our findings unravel a new role for BDNF, suggesting that this neurotrophin is necessary and sufficient to maintain the reactivated fear extinction engram.
Fil: Radiske, Andressa. Universidade Federal do Rio Grande; Brasil
Fil: Rossato, Janine Inez. Universidade Federal do Rio Grande; Brasil
Fil: Köhler, Cristiano A.. Universidade Federal do Rio Grande; Brasil
Fil: Gonzalez, Maria Carolina. Universidade Federal do Rio Grande; Brasil
Fil: Medina, Jorge Horacio. Universidade Federal do Rio Grande; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; Argentina
Fil: Cammarota, Martin Pablo. Universidade Federal do Rio Grande; Brasil
Materia
Bdnf
Extinction
Hippocampus
Learning
Memory
Reconsolidation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39089
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Requirement for BDNF in the reconsolidation of fear extinctionRadiske, AndressaRossato, Janine InezKöhler, Cristiano A.Gonzalez, Maria CarolinaMedina, Jorge HoracioCammarota, Martin PabloBdnfExtinctionHippocampusLearningMemoryReconsolidationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Therapies based on the impairment of reconsolidation or the enhancement of extinction offer the possibility of decreasing the persistent recollection of distressing memories. However, the direct interplay between reconsolidation and extinction has rarely been considered. Previously, we reported that reactivation induces reconsolidation of fear extinction memory. Here, using a step-down inhibitory avoidance learning paradigm in rats, we show that intrahippocampus infusion of function-blocking anti-BDNF antibody immediately or 6 h after extinction memory reactivation impairs the reconsolidation of extinction. Extinction memory reactivation increases proBDNF, BDNF, and tropomyosin receptor kinase B (TrkB) phosphorylation levels in dorsal CA1, while blocking BDNF maturation in the hippocampus with plasminogen activator inhibitor 1 hinders the persistence of extinction and induces the recurrence of fear. Moreover, coinfusion of recombinant BDNF (0.25 μg/side) after extinction memory reactivation impedes the recovery of the avoidance response induced by inhibiting gene expression and protein synthesis in the dorsal hippocampus. Our findings unravel a new role for BDNF, suggesting that this neurotrophin is necessary and sufficient to maintain the reactivated fear extinction engram.Fil: Radiske, Andressa. Universidade Federal do Rio Grande; BrasilFil: Rossato, Janine Inez. Universidade Federal do Rio Grande; BrasilFil: Köhler, Cristiano A.. Universidade Federal do Rio Grande; BrasilFil: Gonzalez, Maria Carolina. Universidade Federal do Rio Grande; BrasilFil: Medina, Jorge Horacio. Universidade Federal do Rio Grande; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; ArgentinaFil: Cammarota, Martin Pablo. Universidade Federal do Rio Grande; BrasilSociety for Neuroscience2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39089Radiske, Andressa; Rossato, Janine Inez; Köhler, Cristiano A.; Gonzalez, Maria Carolina; Medina, Jorge Horacio; et al.; Requirement for BDNF in the reconsolidation of fear extinction; Society for Neuroscience; Journal of Neuroscience; 35; 16; 4-2015; 6570-65740270-6474CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.4093-14.2015info:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/35/16/6570info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:20:41Zoai:ri.conicet.gov.ar:11336/39089instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:20:41.734CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Requirement for BDNF in the reconsolidation of fear extinction
title Requirement for BDNF in the reconsolidation of fear extinction
spellingShingle Requirement for BDNF in the reconsolidation of fear extinction
Radiske, Andressa
Bdnf
Extinction
Hippocampus
Learning
Memory
Reconsolidation
title_short Requirement for BDNF in the reconsolidation of fear extinction
title_full Requirement for BDNF in the reconsolidation of fear extinction
title_fullStr Requirement for BDNF in the reconsolidation of fear extinction
title_full_unstemmed Requirement for BDNF in the reconsolidation of fear extinction
title_sort Requirement for BDNF in the reconsolidation of fear extinction
dc.creator.none.fl_str_mv Radiske, Andressa
Rossato, Janine Inez
Köhler, Cristiano A.
Gonzalez, Maria Carolina
Medina, Jorge Horacio
Cammarota, Martin Pablo
author Radiske, Andressa
author_facet Radiske, Andressa
Rossato, Janine Inez
Köhler, Cristiano A.
Gonzalez, Maria Carolina
Medina, Jorge Horacio
Cammarota, Martin Pablo
author_role author
author2 Rossato, Janine Inez
Köhler, Cristiano A.
Gonzalez, Maria Carolina
Medina, Jorge Horacio
Cammarota, Martin Pablo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bdnf
Extinction
Hippocampus
Learning
Memory
Reconsolidation
topic Bdnf
Extinction
Hippocampus
Learning
Memory
Reconsolidation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Therapies based on the impairment of reconsolidation or the enhancement of extinction offer the possibility of decreasing the persistent recollection of distressing memories. However, the direct interplay between reconsolidation and extinction has rarely been considered. Previously, we reported that reactivation induces reconsolidation of fear extinction memory. Here, using a step-down inhibitory avoidance learning paradigm in rats, we show that intrahippocampus infusion of function-blocking anti-BDNF antibody immediately or 6 h after extinction memory reactivation impairs the reconsolidation of extinction. Extinction memory reactivation increases proBDNF, BDNF, and tropomyosin receptor kinase B (TrkB) phosphorylation levels in dorsal CA1, while blocking BDNF maturation in the hippocampus with plasminogen activator inhibitor 1 hinders the persistence of extinction and induces the recurrence of fear. Moreover, coinfusion of recombinant BDNF (0.25 μg/side) after extinction memory reactivation impedes the recovery of the avoidance response induced by inhibiting gene expression and protein synthesis in the dorsal hippocampus. Our findings unravel a new role for BDNF, suggesting that this neurotrophin is necessary and sufficient to maintain the reactivated fear extinction engram.
Fil: Radiske, Andressa. Universidade Federal do Rio Grande; Brasil
Fil: Rossato, Janine Inez. Universidade Federal do Rio Grande; Brasil
Fil: Köhler, Cristiano A.. Universidade Federal do Rio Grande; Brasil
Fil: Gonzalez, Maria Carolina. Universidade Federal do Rio Grande; Brasil
Fil: Medina, Jorge Horacio. Universidade Federal do Rio Grande; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; Argentina
Fil: Cammarota, Martin Pablo. Universidade Federal do Rio Grande; Brasil
description Therapies based on the impairment of reconsolidation or the enhancement of extinction offer the possibility of decreasing the persistent recollection of distressing memories. However, the direct interplay between reconsolidation and extinction has rarely been considered. Previously, we reported that reactivation induces reconsolidation of fear extinction memory. Here, using a step-down inhibitory avoidance learning paradigm in rats, we show that intrahippocampus infusion of function-blocking anti-BDNF antibody immediately or 6 h after extinction memory reactivation impairs the reconsolidation of extinction. Extinction memory reactivation increases proBDNF, BDNF, and tropomyosin receptor kinase B (TrkB) phosphorylation levels in dorsal CA1, while blocking BDNF maturation in the hippocampus with plasminogen activator inhibitor 1 hinders the persistence of extinction and induces the recurrence of fear. Moreover, coinfusion of recombinant BDNF (0.25 μg/side) after extinction memory reactivation impedes the recovery of the avoidance response induced by inhibiting gene expression and protein synthesis in the dorsal hippocampus. Our findings unravel a new role for BDNF, suggesting that this neurotrophin is necessary and sufficient to maintain the reactivated fear extinction engram.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39089
Radiske, Andressa; Rossato, Janine Inez; Köhler, Cristiano A.; Gonzalez, Maria Carolina; Medina, Jorge Horacio; et al.; Requirement for BDNF in the reconsolidation of fear extinction; Society for Neuroscience; Journal of Neuroscience; 35; 16; 4-2015; 6570-6574
0270-6474
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39089
identifier_str_mv Radiske, Andressa; Rossato, Janine Inez; Köhler, Cristiano A.; Gonzalez, Maria Carolina; Medina, Jorge Horacio; et al.; Requirement for BDNF in the reconsolidation of fear extinction; Society for Neuroscience; Journal of Neuroscience; 35; 16; 4-2015; 6570-6574
0270-6474
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1523/JNEUROSCI.4093-14.2015
info:eu-repo/semantics/altIdentifier/url/http://www.jneurosci.org/content/35/16/6570
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for Neuroscience
publisher.none.fl_str_mv Society for Neuroscience
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981131752833024
score 12.48226

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