In vitro immune response induced by a Salmonella Typhimurium mutant

Autores
Torres, Mariela Analía; Leccese Terraf, Maria Cecilia; de Moreno, Maria Alejandra; Minahk, Carlos Javier; Delgado, Monica Alejandra
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Salmonella genus is responsible for gastrointestinal diseases and is a pathogen with great prevalence among argentine population. The infection capacity of Salmonella depends on the genes expression under different regulatory systems. The RcsCDB system regulates genes involved in virulence, such as those of colanic acid biosynthesis and flagellar genes, among others. This uncommon regulatory system is constitutively activated in Salmonella Typhimurium rcsC11 strain since the RcsB regulator is not dephosphorylated by the mutated RcsC sensor. As a consequence, rcsC11 strain presents a mucoid phenotype, loss of motility and virulence attenuation.The aim of this study was to determine differences in the host immune response induced by the rcsC11 and wild type strains. For this purpose, the in vitro infection assay was performed with two relevant cell lines. On the one hand, intestinal epithelial cells (Caco-2), as an initial barrier to the enteropathogen infection. On the other hand, macrophage cells (Raw264.7) which plays a principal role in the immune response. The gentamicin protection assay was performed and the cell culture supernatant was taken out at different stages infection. This supernatant was used to determine the presence of extracellular cytokines by flow-cytometry.The results showed that rcsC11 mutant induced the IL-6, IL-10 and TNF cytokine secretion at same level than the wild type strain. However, the IL-8 cytokine levels secreted by Caco-2 cells showed difference between rcsC11 and wild type strain at 18 h post infection. These results demonstrated that the rcsC11 strain, is able to produce the same immune response than the wild type strain, even when displays an attenuated phenotype and a deficient replication within the host. All the data allow us to propose that rcsC11 could be a candidate for the development of attenuated vaccines against this enteropathogenic bacterium.
Fil: Torres, Mariela Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Leccese Terraf, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Minahk, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology and XV Annual Meeting Argentinean Society for General Microbiology
Buenos Aires
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
Materia
SALMONELLA
ENTEROPATOGENO
INFECCION
RESPUESTA INMUNE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181502
Acceder
id CONICETDig_c51e76e5de7af605c507076f70262b4a
oai_identifier_str oai:ri.conicet.gov.ar:11336/181502
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro immune response induced by a Salmonella Typhimurium mutantTorres, Mariela AnalíaLeccese Terraf, Maria Ceciliade Moreno, Maria AlejandraMinahk, Carlos JavierDelgado, Monica AlejandraSALMONELLAENTEROPATOGENOINFECCIONRESPUESTA INMUNEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Salmonella genus is responsible for gastrointestinal diseases and is a pathogen with great prevalence among argentine population. The infection capacity of Salmonella depends on the genes expression under different regulatory systems. The RcsCDB system regulates genes involved in virulence, such as those of colanic acid biosynthesis and flagellar genes, among others. This uncommon regulatory system is constitutively activated in Salmonella Typhimurium rcsC11 strain since the RcsB regulator is not dephosphorylated by the mutated RcsC sensor. As a consequence, rcsC11 strain presents a mucoid phenotype, loss of motility and virulence attenuation.The aim of this study was to determine differences in the host immune response induced by the rcsC11 and wild type strains. For this purpose, the in vitro infection assay was performed with two relevant cell lines. On the one hand, intestinal epithelial cells (Caco-2), as an initial barrier to the enteropathogen infection. On the other hand, macrophage cells (Raw264.7) which plays a principal role in the immune response. The gentamicin protection assay was performed and the cell culture supernatant was taken out at different stages infection. This supernatant was used to determine the presence of extracellular cytokines by flow-cytometry.The results showed that rcsC11 mutant induced the IL-6, IL-10 and TNF cytokine secretion at same level than the wild type strain. However, the IL-8 cytokine levels secreted by Caco-2 cells showed difference between rcsC11 and wild type strain at 18 h post infection. These results demonstrated that the rcsC11 strain, is able to produce the same immune response than the wild type strain, even when displays an attenuated phenotype and a deficient replication within the host. All the data allow us to propose that rcsC11 could be a candidate for the development of attenuated vaccines against this enteropathogenic bacterium.Fil: Torres, Mariela Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Leccese Terraf, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Minahk, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaLVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology and XV Annual Meeting Argentinean Society for General MicrobiologyBuenos AiresArgentinaSociedad Argentina de Investigación Bioquímica y Biología MolecularSociedad Argentina de Microbiología GeneralTech Science Press2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181502In vitro immune response induced by a Salmonella Typhimurium mutant; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology and XV Annual Meeting Argentinean Society for General Microbiology; Buenos Aires; Argentina; 2020; 1-6CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2020-version-final.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:00Zoai:ri.conicet.gov.ar:11336/181502instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:00.946CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro immune response induced by a Salmonella Typhimurium mutant
title In vitro immune response induced by a Salmonella Typhimurium mutant
spellingShingle In vitro immune response induced by a Salmonella Typhimurium mutant
Torres, Mariela Analía
SALMONELLA
ENTEROPATOGENO
INFECCION
RESPUESTA INMUNE
title_short In vitro immune response induced by a Salmonella Typhimurium mutant
title_full In vitro immune response induced by a Salmonella Typhimurium mutant
title_fullStr In vitro immune response induced by a Salmonella Typhimurium mutant
title_full_unstemmed In vitro immune response induced by a Salmonella Typhimurium mutant
title_sort In vitro immune response induced by a Salmonella Typhimurium mutant
dc.creator.none.fl_str_mv Torres, Mariela Analía
Leccese Terraf, Maria Cecilia
de Moreno, Maria Alejandra
Minahk, Carlos Javier
Delgado, Monica Alejandra
author Torres, Mariela Analía
author_facet Torres, Mariela Analía
Leccese Terraf, Maria Cecilia
de Moreno, Maria Alejandra
Minahk, Carlos Javier
Delgado, Monica Alejandra
author_role author
author2 Leccese Terraf, Maria Cecilia
de Moreno, Maria Alejandra
Minahk, Carlos Javier
Delgado, Monica Alejandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv SALMONELLA
ENTEROPATOGENO
INFECCION
RESPUESTA INMUNE
topic SALMONELLA
ENTEROPATOGENO
INFECCION
RESPUESTA INMUNE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Salmonella genus is responsible for gastrointestinal diseases and is a pathogen with great prevalence among argentine population. The infection capacity of Salmonella depends on the genes expression under different regulatory systems. The RcsCDB system regulates genes involved in virulence, such as those of colanic acid biosynthesis and flagellar genes, among others. This uncommon regulatory system is constitutively activated in Salmonella Typhimurium rcsC11 strain since the RcsB regulator is not dephosphorylated by the mutated RcsC sensor. As a consequence, rcsC11 strain presents a mucoid phenotype, loss of motility and virulence attenuation.The aim of this study was to determine differences in the host immune response induced by the rcsC11 and wild type strains. For this purpose, the in vitro infection assay was performed with two relevant cell lines. On the one hand, intestinal epithelial cells (Caco-2), as an initial barrier to the enteropathogen infection. On the other hand, macrophage cells (Raw264.7) which plays a principal role in the immune response. The gentamicin protection assay was performed and the cell culture supernatant was taken out at different stages infection. This supernatant was used to determine the presence of extracellular cytokines by flow-cytometry.The results showed that rcsC11 mutant induced the IL-6, IL-10 and TNF cytokine secretion at same level than the wild type strain. However, the IL-8 cytokine levels secreted by Caco-2 cells showed difference between rcsC11 and wild type strain at 18 h post infection. These results demonstrated that the rcsC11 strain, is able to produce the same immune response than the wild type strain, even when displays an attenuated phenotype and a deficient replication within the host. All the data allow us to propose that rcsC11 could be a candidate for the development of attenuated vaccines against this enteropathogenic bacterium.
Fil: Torres, Mariela Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Leccese Terraf, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Minahk, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Delgado, Monica Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology and XV Annual Meeting Argentinean Society for General Microbiology
Buenos Aires
Argentina
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
description Salmonella genus is responsible for gastrointestinal diseases and is a pathogen with great prevalence among argentine population. The infection capacity of Salmonella depends on the genes expression under different regulatory systems. The RcsCDB system regulates genes involved in virulence, such as those of colanic acid biosynthesis and flagellar genes, among others. This uncommon regulatory system is constitutively activated in Salmonella Typhimurium rcsC11 strain since the RcsB regulator is not dephosphorylated by the mutated RcsC sensor. As a consequence, rcsC11 strain presents a mucoid phenotype, loss of motility and virulence attenuation.The aim of this study was to determine differences in the host immune response induced by the rcsC11 and wild type strains. For this purpose, the in vitro infection assay was performed with two relevant cell lines. On the one hand, intestinal epithelial cells (Caco-2), as an initial barrier to the enteropathogen infection. On the other hand, macrophage cells (Raw264.7) which plays a principal role in the immune response. The gentamicin protection assay was performed and the cell culture supernatant was taken out at different stages infection. This supernatant was used to determine the presence of extracellular cytokines by flow-cytometry.The results showed that rcsC11 mutant induced the IL-6, IL-10 and TNF cytokine secretion at same level than the wild type strain. However, the IL-8 cytokine levels secreted by Caco-2 cells showed difference between rcsC11 and wild type strain at 18 h post infection. These results demonstrated that the rcsC11 strain, is able to produce the same immune response than the wild type strain, even when displays an attenuated phenotype and a deficient replication within the host. All the data allow us to propose that rcsC11 could be a candidate for the development of attenuated vaccines against this enteropathogenic bacterium.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181502
In vitro immune response induced by a Salmonella Typhimurium mutant; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology and XV Annual Meeting Argentinean Society for General Microbiology; Buenos Aires; Argentina; 2020; 1-6
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181502
identifier_str_mv In vitro immune response induced by a Salmonella Typhimurium mutant; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology and XV Annual Meeting Argentinean Society for General Microbiology; Buenos Aires; Argentina; 2020; 1-6
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2020-version-final.pdf
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
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reponame_str CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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