Role of curing agents in the adaptive Rresponse of the bioprotective Latilactobacillus curvatus CRL 705 from a physiologic and proteomic perspective

Autores
Teran, Lucrecia Cecilia; Orihuel, Alejandra; Bentencourt, Emilse Viviana; Raya, Raul Ricardo; Fadda, Silvina G.
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
During meat processing, lactic acid bacteria (LAB) have to competitively adapt to the hostile environment produced by curing additives (CA). The objective of this study was to investigate the ability of Latilactobacillus curvatus CRL 705, a bioprotective strain of meat origin, to adapt to CA. A physiological and proteomic approach was performed. CRL 705 was grown in a chemically defined medium (CDM) containing specific concentrations of CA (NaCl, nitrite, sucrose, and ascorbic acid). The results showed minor differences in growth kinetics in the presence of CA. Glucose consumption, present in the CDM, and production of lactic acid and bacteriocins were not significantly affected. Proteomic analyses indicated that most of the identified proteins (36 out of 39) mainly related to carbohydrate metabolism (18%), posttranslational modifications (15.6%), energy production and conversion (11.1%), translation (11.1%), and nucleotide metabolism (8.9%) were underexpressed. In response to the studied CA, CRL 705 slowed down its general metabolism, achieving slight changes in physiological and proteomic parameters. The observed performance is another characteristic that extends the well-known competitive profile of CRL 705 as a meat starter and bioprotective culture. This is the first report dealing with the impact of CA on LAB proteomics.
Fil: Teran, Lucrecia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Orihuel, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Bentencourt, Emilse Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Fadda, Silvina G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
LACTIC ACID BACTERIA
CURING CONDITIONS
FERMENTED SAUSAGES
PROTEOMICS
BIOPROTECTIVE CULTURES
MEAT PRODUCTS
MEAT PROCESSING CONDITIONS
CURING AGENTS
ADAPTIVE RESPONSE
PROTEOMICS
DIFFERENTIAL PROTEIN EXPRESSION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/249543
Acceder
id CONICETDig_c4b6d4750832c4882159ab3e88392b98
oai_identifier_str oai:ri.conicet.gov.ar:11336/249543
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of curing agents in the adaptive Rresponse of the bioprotective Latilactobacillus curvatus CRL 705 from a physiologic and proteomic perspectiveTeran, Lucrecia CeciliaOrihuel, AlejandraBentencourt, Emilse VivianaRaya, Raul RicardoFadda, Silvina G.LACTIC ACID BACTERIACURING CONDITIONSFERMENTED SAUSAGESPROTEOMICSBIOPROTECTIVE CULTURESMEAT PRODUCTSMEAT PROCESSING CONDITIONSCURING AGENTSADAPTIVE RESPONSEPROTEOMICSDIFFERENTIAL PROTEIN EXPRESSIONhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2During meat processing, lactic acid bacteria (LAB) have to competitively adapt to the hostile environment produced by curing additives (CA). The objective of this study was to investigate the ability of Latilactobacillus curvatus CRL 705, a bioprotective strain of meat origin, to adapt to CA. A physiological and proteomic approach was performed. CRL 705 was grown in a chemically defined medium (CDM) containing specific concentrations of CA (NaCl, nitrite, sucrose, and ascorbic acid). The results showed minor differences in growth kinetics in the presence of CA. Glucose consumption, present in the CDM, and production of lactic acid and bacteriocins were not significantly affected. Proteomic analyses indicated that most of the identified proteins (36 out of 39) mainly related to carbohydrate metabolism (18%), posttranslational modifications (15.6%), energy production and conversion (11.1%), translation (11.1%), and nucleotide metabolism (8.9%) were underexpressed. In response to the studied CA, CRL 705 slowed down its general metabolism, achieving slight changes in physiological and proteomic parameters. The observed performance is another characteristic that extends the well-known competitive profile of CRL 705 as a meat starter and bioprotective culture. This is the first report dealing with the impact of CA on LAB proteomics.Fil: Teran, Lucrecia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Orihuel, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Bentencourt, Emilse Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Fadda, Silvina G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaMolecular Diversity Preservation International2023-11-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/249543Teran, Lucrecia Cecilia; Orihuel, Alejandra; Bentencourt, Emilse Viviana; Raya, Raul Ricardo; Fadda, Silvina G.; Role of curing agents in the adaptive Rresponse of the bioprotective Latilactobacillus curvatus CRL 705 from a physiologic and proteomic perspective; Molecular Diversity Preservation International; Bacteria; 2; 4; 2-11-2023; 142-1542674-1334CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2674-1334/2/4/11info:eu-repo/semantics/altIdentifier/doi/10.3390/bacteria2040011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:37Zoai:ri.conicet.gov.ar:11336/249543instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:37.382CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of curing agents in the adaptive Rresponse of the bioprotective Latilactobacillus curvatus CRL 705 from a physiologic and proteomic perspective
title Role of curing agents in the adaptive Rresponse of the bioprotective Latilactobacillus curvatus CRL 705 from a physiologic and proteomic perspective
spellingShingle Role of curing agents in the adaptive Rresponse of the bioprotective Latilactobacillus curvatus CRL 705 from a physiologic and proteomic perspective
Teran, Lucrecia Cecilia
LACTIC ACID BACTERIA
CURING CONDITIONS
FERMENTED SAUSAGES
PROTEOMICS
BIOPROTECTIVE CULTURES
MEAT PRODUCTS
MEAT PROCESSING CONDITIONS
CURING AGENTS
ADAPTIVE RESPONSE
PROTEOMICS
DIFFERENTIAL PROTEIN EXPRESSION
title_short Role of curing agents in the adaptive Rresponse of the bioprotective Latilactobacillus curvatus CRL 705 from a physiologic and proteomic perspective
title_full Role of curing agents in the adaptive Rresponse of the bioprotective Latilactobacillus curvatus CRL 705 from a physiologic and proteomic perspective
title_fullStr Role of curing agents in the adaptive Rresponse of the bioprotective Latilactobacillus curvatus CRL 705 from a physiologic and proteomic perspective
title_full_unstemmed Role of curing agents in the adaptive Rresponse of the bioprotective Latilactobacillus curvatus CRL 705 from a physiologic and proteomic perspective
title_sort Role of curing agents in the adaptive Rresponse of the bioprotective Latilactobacillus curvatus CRL 705 from a physiologic and proteomic perspective
dc.creator.none.fl_str_mv Teran, Lucrecia Cecilia
Orihuel, Alejandra
Bentencourt, Emilse Viviana
Raya, Raul Ricardo
Fadda, Silvina G.
author Teran, Lucrecia Cecilia
author_facet Teran, Lucrecia Cecilia
Orihuel, Alejandra
Bentencourt, Emilse Viviana
Raya, Raul Ricardo
Fadda, Silvina G.
author_role author
author2 Orihuel, Alejandra
Bentencourt, Emilse Viviana
Raya, Raul Ricardo
Fadda, Silvina G.
author2_role author
author
author
author
dc.subject.none.fl_str_mv LACTIC ACID BACTERIA
CURING CONDITIONS
FERMENTED SAUSAGES
PROTEOMICS
BIOPROTECTIVE CULTURES
MEAT PRODUCTS
MEAT PROCESSING CONDITIONS
CURING AGENTS
ADAPTIVE RESPONSE
PROTEOMICS
DIFFERENTIAL PROTEIN EXPRESSION
topic LACTIC ACID BACTERIA
CURING CONDITIONS
FERMENTED SAUSAGES
PROTEOMICS
BIOPROTECTIVE CULTURES
MEAT PRODUCTS
MEAT PROCESSING CONDITIONS
CURING AGENTS
ADAPTIVE RESPONSE
PROTEOMICS
DIFFERENTIAL PROTEIN EXPRESSION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv During meat processing, lactic acid bacteria (LAB) have to competitively adapt to the hostile environment produced by curing additives (CA). The objective of this study was to investigate the ability of Latilactobacillus curvatus CRL 705, a bioprotective strain of meat origin, to adapt to CA. A physiological and proteomic approach was performed. CRL 705 was grown in a chemically defined medium (CDM) containing specific concentrations of CA (NaCl, nitrite, sucrose, and ascorbic acid). The results showed minor differences in growth kinetics in the presence of CA. Glucose consumption, present in the CDM, and production of lactic acid and bacteriocins were not significantly affected. Proteomic analyses indicated that most of the identified proteins (36 out of 39) mainly related to carbohydrate metabolism (18%), posttranslational modifications (15.6%), energy production and conversion (11.1%), translation (11.1%), and nucleotide metabolism (8.9%) were underexpressed. In response to the studied CA, CRL 705 slowed down its general metabolism, achieving slight changes in physiological and proteomic parameters. The observed performance is another characteristic that extends the well-known competitive profile of CRL 705 as a meat starter and bioprotective culture. This is the first report dealing with the impact of CA on LAB proteomics.
Fil: Teran, Lucrecia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Orihuel, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Bentencourt, Emilse Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Raya, Raul Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Fadda, Silvina G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description During meat processing, lactic acid bacteria (LAB) have to competitively adapt to the hostile environment produced by curing additives (CA). The objective of this study was to investigate the ability of Latilactobacillus curvatus CRL 705, a bioprotective strain of meat origin, to adapt to CA. A physiological and proteomic approach was performed. CRL 705 was grown in a chemically defined medium (CDM) containing specific concentrations of CA (NaCl, nitrite, sucrose, and ascorbic acid). The results showed minor differences in growth kinetics in the presence of CA. Glucose consumption, present in the CDM, and production of lactic acid and bacteriocins were not significantly affected. Proteomic analyses indicated that most of the identified proteins (36 out of 39) mainly related to carbohydrate metabolism (18%), posttranslational modifications (15.6%), energy production and conversion (11.1%), translation (11.1%), and nucleotide metabolism (8.9%) were underexpressed. In response to the studied CA, CRL 705 slowed down its general metabolism, achieving slight changes in physiological and proteomic parameters. The observed performance is another characteristic that extends the well-known competitive profile of CRL 705 as a meat starter and bioprotective culture. This is the first report dealing with the impact of CA on LAB proteomics.
publishDate 2023
dc.date.none.fl_str_mv 2023-11-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/249543
Teran, Lucrecia Cecilia; Orihuel, Alejandra; Bentencourt, Emilse Viviana; Raya, Raul Ricardo; Fadda, Silvina G.; Role of curing agents in the adaptive Rresponse of the bioprotective Latilactobacillus curvatus CRL 705 from a physiologic and proteomic perspective; Molecular Diversity Preservation International; Bacteria; 2; 4; 2-11-2023; 142-154
2674-1334
CONICET Digital
CONICET
url http://hdl.handle.net/11336/249543
identifier_str_mv Teran, Lucrecia Cecilia; Orihuel, Alejandra; Bentencourt, Emilse Viviana; Raya, Raul Ricardo; Fadda, Silvina G.; Role of curing agents in the adaptive Rresponse of the bioprotective Latilactobacillus curvatus CRL 705 from a physiologic and proteomic perspective; Molecular Diversity Preservation International; Bacteria; 2; 4; 2-11-2023; 142-154
2674-1334
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2674-1334/2/4/11
info:eu-repo/semantics/altIdentifier/doi/10.3390/bacteria2040011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269413385961472
score 13.13397

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