Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions

Autores: Acosta Rodríguez, Victoria América; Marquez, Sebastian; Salvador, Gabriela Alejandra; Pasquaré, Susana Juana; Gorne, Lucas Damián; Garbarino Pico, Eduardo; Giusto, Norma Maria; Guido, Mario Eduardo
Año de publicación: 2013
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Circadian clocks regulate the temporal organization of several biochemical processes including lipid metabolism and their disruption leads to severe metabolic disorders. Immortalized cell lines acting as circadian clocks display daily variations in [32P]-phospholipid labeling; however, the regulation of glycerophospholipid (GPL) synthesis by internal clocks remains unknown. Here we found that arrested NIH 3T3 cells synchronized with a 2 h-serum shock exhibited temporal oscillations in: a) the labeling of total [3H]- GPLs, with lowest levels around 28 and 56 h, and b) the activity of GPL-synthesizing and -remodeling enzymes such as phosphatidate phosphohydrolase 1 (PAP-1) and lysophospholipid acyltransferases (LPLAT) respectively, with antiphase profiles. In addition, we investigated the temporal regulation of phosphatidylcholine (PC) biosynthesis. PC is mainly synthesized through the Kennedy pathway with Choline Kinase (ChoK) and CTP:phosphocholine cytidylyltranferase (CCT) as key regulatory enzymes. We observed that the PC labeling exhibited daily changes with the lowest levels every ~28 h that were accompanied by brief increases in CCT activity and the oscillation in ChoK mRNA expression and activity. Results demonstrate that the metabolisms of GPLs and particularly of PC in synchronized fibroblasts are subject to a complex temporal control involving concerted changes in the expression and/or activities of specific synthesizing enzymes.
Fil: Acosta Rodríguez, Victoria América. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
Fil: Marquez, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina
Fil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina
Fil: Gorne, Lucas Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
Fil: Garbarino Pico, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina
Fil: Guido, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
Materia: Phospholipid
Circadian Rhythm
Fibroblast
Serum Shock
Synchronization
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/4648

Acceder

id	CONICETDig_c41afeac8e3479021978e84a6b4c1334
oai_identifier_str	oai:ri.conicet.gov.ar:11336/4648
network_acronym_str	CONICETDig
repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributionsAcosta Rodríguez, Victoria AméricaMarquez, SebastianSalvador, Gabriela AlejandraPasquaré, Susana JuanaGorne, Lucas DamiánGarbarino Pico, EduardoGiusto, Norma MariaGuido, Mario EduardoPhospholipidCircadian RhythmFibroblastSerum ShockSynchronizationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Circadian clocks regulate the temporal organization of several biochemical processes including lipid metabolism and their disruption leads to severe metabolic disorders. Immortalized cell lines acting as circadian clocks display daily variations in [32P]-phospholipid labeling; however, the regulation of glycerophospholipid (GPL) synthesis by internal clocks remains unknown. Here we found that arrested NIH 3T3 cells synchronized with a 2 h-serum shock exhibited temporal oscillations in: a) the labeling of total [3H]- GPLs, with lowest levels around 28 and 56 h, and b) the activity of GPL-synthesizing and -remodeling enzymes such as phosphatidate phosphohydrolase 1 (PAP-1) and lysophospholipid acyltransferases (LPLAT) respectively, with antiphase profiles. In addition, we investigated the temporal regulation of phosphatidylcholine (PC) biosynthesis. PC is mainly synthesized through the Kennedy pathway with Choline Kinase (ChoK) and CTP:phosphocholine cytidylyltranferase (CCT) as key regulatory enzymes. We observed that the PC labeling exhibited daily changes with the lowest levels every ~28 h that were accompanied by brief increases in CCT activity and the oscillation in ChoK mRNA expression and activity. Results demonstrate that the metabolisms of GPLs and particularly of PC in synchronized fibroblasts are subject to a complex temporal control involving concerted changes in the expression and/or activities of specific synthesizing enzymes.Fil: Acosta Rodríguez, Victoria América. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); ArgentinaFil: Marquez, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); ArgentinaFil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); ArgentinaFil: Gorne, Lucas Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); ArgentinaFil: Garbarino Pico, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); ArgentinaFil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); ArgentinaFil: Guido, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); ArgentinaAmerican Society for Biochemistry and Molecular Biology2013-05-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4648Acosta Rodríguez, Victoria América; Marquez, Sebastian; Salvador, Gabriela Alejandra; Pasquaré, Susana Juana; Gorne, Lucas Damián; et al.; Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions; American Society for Biochemistry and Molecular Biology; Journal of Lipid Research; 54; 7; 2-5-2013; 1798-18110022-22751539-7262enginfo:eu-repo/semantics/altIdentifier/doi/10.1194/jlr.M034264info:eu-repo/semantics/altIdentifier/url/http://www.jlr.org/content/54/7/1798info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679383/info:eu-repo/semantics/altIdentifier/issn/0022-2275info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:16:43Zoai:ri.conicet.gov.ar:11336/4648instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:16:43.693CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions
title	Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions
spellingShingle	Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions Acosta Rodríguez, Victoria América Phospholipid Circadian Rhythm Fibroblast Serum Shock Synchronization
title_short	Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions
title_full	Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions
title_fullStr	Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions
title_full_unstemmed	Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions
title_sort	Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions
dc.creator.none.fl_str_mv	Acosta Rodríguez, Victoria América Marquez, Sebastian Salvador, Gabriela Alejandra Pasquaré, Susana Juana Gorne, Lucas Damián Garbarino Pico, Eduardo Giusto, Norma Maria Guido, Mario Eduardo
author	Acosta Rodríguez, Victoria América
author_facet	Acosta Rodríguez, Victoria América Marquez, Sebastian Salvador, Gabriela Alejandra Pasquaré, Susana Juana Gorne, Lucas Damián Garbarino Pico, Eduardo Giusto, Norma Maria Guido, Mario Eduardo
author_role	author
author2	Marquez, Sebastian Salvador, Gabriela Alejandra Pasquaré, Susana Juana Gorne, Lucas Damián Garbarino Pico, Eduardo Giusto, Norma Maria Guido, Mario Eduardo
author2_role	author author author author author author author
dc.subject.none.fl_str_mv	Phospholipid Circadian Rhythm Fibroblast Serum Shock Synchronization
topic	Phospholipid Circadian Rhythm Fibroblast Serum Shock Synchronization
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	Circadian clocks regulate the temporal organization of several biochemical processes including lipid metabolism and their disruption leads to severe metabolic disorders. Immortalized cell lines acting as circadian clocks display daily variations in [32P]-phospholipid labeling; however, the regulation of glycerophospholipid (GPL) synthesis by internal clocks remains unknown. Here we found that arrested NIH 3T3 cells synchronized with a 2 h-serum shock exhibited temporal oscillations in: a) the labeling of total [3H]- GPLs, with lowest levels around 28 and 56 h, and b) the activity of GPL-synthesizing and -remodeling enzymes such as phosphatidate phosphohydrolase 1 (PAP-1) and lysophospholipid acyltransferases (LPLAT) respectively, with antiphase profiles. In addition, we investigated the temporal regulation of phosphatidylcholine (PC) biosynthesis. PC is mainly synthesized through the Kennedy pathway with Choline Kinase (ChoK) and CTP:phosphocholine cytidylyltranferase (CCT) as key regulatory enzymes. We observed that the PC labeling exhibited daily changes with the lowest levels every ~28 h that were accompanied by brief increases in CCT activity and the oscillation in ChoK mRNA expression and activity. Results demonstrate that the metabolisms of GPLs and particularly of PC in synchronized fibroblasts are subject to a complex temporal control involving concerted changes in the expression and/or activities of specific synthesizing enzymes. Fil: Acosta Rodríguez, Victoria América. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina Fil: Marquez, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina Fil: Pasquaré, Susana Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina Fil: Gorne, Lucas Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina Fil: Garbarino Pico, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Investigaciones Bioquímicas Bahía Blanca (i); Argentina Fil: Guido, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina
description	Circadian clocks regulate the temporal organization of several biochemical processes including lipid metabolism and their disruption leads to severe metabolic disorders. Immortalized cell lines acting as circadian clocks display daily variations in [32P]-phospholipid labeling; however, the regulation of glycerophospholipid (GPL) synthesis by internal clocks remains unknown. Here we found that arrested NIH 3T3 cells synchronized with a 2 h-serum shock exhibited temporal oscillations in: a) the labeling of total [3H]- GPLs, with lowest levels around 28 and 56 h, and b) the activity of GPL-synthesizing and -remodeling enzymes such as phosphatidate phosphohydrolase 1 (PAP-1) and lysophospholipid acyltransferases (LPLAT) respectively, with antiphase profiles. In addition, we investigated the temporal regulation of phosphatidylcholine (PC) biosynthesis. PC is mainly synthesized through the Kennedy pathway with Choline Kinase (ChoK) and CTP:phosphocholine cytidylyltranferase (CCT) as key regulatory enzymes. We observed that the PC labeling exhibited daily changes with the lowest levels every ~28 h that were accompanied by brief increases in CCT activity and the oscillation in ChoK mRNA expression and activity. Results demonstrate that the metabolisms of GPLs and particularly of PC in synchronized fibroblasts are subject to a complex temporal control involving concerted changes in the expression and/or activities of specific synthesizing enzymes.
publishDate	2013
dc.date.none.fl_str_mv	2013-05-02
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/4648 Acosta Rodríguez, Victoria América; Marquez, Sebastian; Salvador, Gabriela Alejandra; Pasquaré, Susana Juana; Gorne, Lucas Damián; et al.; Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions; American Society for Biochemistry and Molecular Biology; Journal of Lipid Research; 54; 7; 2-5-2013; 1798-1811 0022-2275 1539-7262
url	http://hdl.handle.net/11336/4648
identifier_str_mv	Acosta Rodríguez, Victoria América; Marquez, Sebastian; Salvador, Gabriela Alejandra; Pasquaré, Susana Juana; Gorne, Lucas Damián; et al.; Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions; American Society for Biochemistry and Molecular Biology; Journal of Lipid Research; 54; 7; 2-5-2013; 1798-1811 0022-2275 1539-7262
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.1194/jlr.M034264 info:eu-repo/semantics/altIdentifier/url/http://www.jlr.org/content/54/7/1798 info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679383/ info:eu-repo/semantics/altIdentifier/issn/0022-2275
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv	American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_	1842980911874834432
score	12.993085

Daily rhythms of glycerophospholipid synthesis in fibroblast cultures involve differential enzyme contributions

Publicaciones similares