Antagonism of Bacillus safensis strain against phytopathogenic bacteria

Autores
Olivella, Laura; Gaido, Jimena Daiana; Torres Manno, Mariano Alberto; Petitti, Tomás Denis; Espariz, Martin; Daurelio, Lucas Damian
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Citrus canker caused by Xanthomonas citri subsp. citri (Xcc), is a bacterial disease which affects all the citrics. One alternative to manage it is the use of antagonist bacteria. The aim of this work was to investigate the antagonist activity of Bacillus safensis (S9) against Xcc. The activity was tested by diffusion assays. Xcc and S9 were grown overnight in Luria Bertani (LB) and potato dextrose (PD) medium, respectively, with continuous agitation at 28°C and then, were diluted to a concentration of 108 CFU/mL. Petri dishes were covered with 15 mL of LB-agar containing 100 μL of the Xcc dilution. Once the medium was solidified, 4 μL drops of S9 were inoculated 3 times in each Petri dish, and the experiment was made by triplicate. After 48 hours of incubation at 28°C, the inhibition zone was measured, and the average inhibition area was calculated as IA = average area of the inhibition zone - average area of the colony. A significant inhibition area of 5.18 cm2 was obtained (one-sample t- test, p<0.05). At the same time, diffusion assays with the supernatant were made to prove its inhibitory ability. Petri dishes were prepared as described above. The supernatant was obtained by centrifugation of the S9 culture grown in PD medium, and then by bacteria filtration. Three filter paper discs embedded with the supernatant were placed per Petri dish, by triplicate. The inhibition zone was measured after 48 hours and calculated the IA. A significant inhibition area of 2.29 cm2 was obtained (one-sample t-test, p<0.05). Besides, a study at genomic level comparing S9 with ten Bacillus strains was made. Different clusters of secondary metabolite synthesis pathways were detected, three common with B. velezensis strains (surfactin, basilicin and bacilibactin). These strains were tested as inhibitors of Xcc and they did not show inhibition (Bacillus sp and B. megaterium) or showed less inhibition (B. velezensis). The difference might be on the expression level of the clusters. These results suggest the potential use of S9 as a canker control agent and further studies will be necessary to identify the Xcc- inhibitor metabolite.
Fil: Olivella, Laura. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias Agropecuarias del Litoral. - Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral.; Argentina
Fil: Gaido, Jimena Daiana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias Agropecuarias del Litoral. - Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral.; Argentina
Fil: Torres Manno, Mariano Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Orgánica; Argentina
Fil: Petitti, Tomás Denis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Espariz, Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Daurelio, Lucas Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology
Buenos Aires
Argentina
Sociedad Argentina de Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
Materia
BACILLUS SAFENSIS
ANTAGONISM
PHYTOPATHOGENIC
XANTHOMONAS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/211207
Acceder
id CONICETDig_c3a49f22156f0c59649b0ffdfc9d2624
oai_identifier_str oai:ri.conicet.gov.ar:11336/211207
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antagonism of Bacillus safensis strain against phytopathogenic bacteriaOlivella, LauraGaido, Jimena DaianaTorres Manno, Mariano AlbertoPetitti, Tomás DenisEspariz, MartinDaurelio, Lucas DamianBACILLUS SAFENSISANTAGONISMPHYTOPATHOGENICXANTHOMONAShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Citrus canker caused by Xanthomonas citri subsp. citri (Xcc), is a bacterial disease which affects all the citrics. One alternative to manage it is the use of antagonist bacteria. The aim of this work was to investigate the antagonist activity of Bacillus safensis (S9) against Xcc. The activity was tested by diffusion assays. Xcc and S9 were grown overnight in Luria Bertani (LB) and potato dextrose (PD) medium, respectively, with continuous agitation at 28°C and then, were diluted to a concentration of 108 CFU/mL. Petri dishes were covered with 15 mL of LB-agar containing 100 μL of the Xcc dilution. Once the medium was solidified, 4 μL drops of S9 were inoculated 3 times in each Petri dish, and the experiment was made by triplicate. After 48 hours of incubation at 28°C, the inhibition zone was measured, and the average inhibition area was calculated as IA = average area of the inhibition zone - average area of the colony. A significant inhibition area of 5.18 cm2 was obtained (one-sample t- test, p<0.05). At the same time, diffusion assays with the supernatant were made to prove its inhibitory ability. Petri dishes were prepared as described above. The supernatant was obtained by centrifugation of the S9 culture grown in PD medium, and then by bacteria filtration. Three filter paper discs embedded with the supernatant were placed per Petri dish, by triplicate. The inhibition zone was measured after 48 hours and calculated the IA. A significant inhibition area of 2.29 cm2 was obtained (one-sample t-test, p<0.05). Besides, a study at genomic level comparing S9 with ten Bacillus strains was made. Different clusters of secondary metabolite synthesis pathways were detected, three common with B. velezensis strains (surfactin, basilicin and bacilibactin). These strains were tested as inhibitors of Xcc and they did not show inhibition (Bacillus sp and B. megaterium) or showed less inhibition (B. velezensis). The difference might be on the expression level of the clusters. These results suggest the potential use of S9 as a canker control agent and further studies will be necessary to identify the Xcc- inhibitor metabolite.Fil: Olivella, Laura. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias Agropecuarias del Litoral. - Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral.; ArgentinaFil: Gaido, Jimena Daiana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias Agropecuarias del Litoral. - Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral.; ArgentinaFil: Torres Manno, Mariano Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Orgánica; ArgentinaFil: Petitti, Tomás Denis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Espariz, Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Daurelio, Lucas Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaLVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General MicrobiologyBuenos AiresArgentinaSociedad Argentina de Bioquímica y Biología MolecularSociedad Argentina de Microbiología GeneralTech Science Press2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/211207Antagonism of Bacillus safensis strain against phytopathogenic bacteria; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology; Buenos Aires; Argentina; 2021; 91-920327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://congresos.g2consultora.com/wp-content/uploads/2021/10/Biocell-Preprint-SAIB-SAMIGE-2021.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:22:34Zoai:ri.conicet.gov.ar:11336/211207instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:22:35.117CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antagonism of Bacillus safensis strain against phytopathogenic bacteria
title Antagonism of Bacillus safensis strain against phytopathogenic bacteria
spellingShingle Antagonism of Bacillus safensis strain against phytopathogenic bacteria
Olivella, Laura
BACILLUS SAFENSIS
ANTAGONISM
PHYTOPATHOGENIC
XANTHOMONAS
title_short Antagonism of Bacillus safensis strain against phytopathogenic bacteria
title_full Antagonism of Bacillus safensis strain against phytopathogenic bacteria
title_fullStr Antagonism of Bacillus safensis strain against phytopathogenic bacteria
title_full_unstemmed Antagonism of Bacillus safensis strain against phytopathogenic bacteria
title_sort Antagonism of Bacillus safensis strain against phytopathogenic bacteria
dc.creator.none.fl_str_mv Olivella, Laura
Gaido, Jimena Daiana
Torres Manno, Mariano Alberto
Petitti, Tomás Denis
Espariz, Martin
Daurelio, Lucas Damian
author Olivella, Laura
author_facet Olivella, Laura
Gaido, Jimena Daiana
Torres Manno, Mariano Alberto
Petitti, Tomás Denis
Espariz, Martin
Daurelio, Lucas Damian
author_role author
author2 Gaido, Jimena Daiana
Torres Manno, Mariano Alberto
Petitti, Tomás Denis
Espariz, Martin
Daurelio, Lucas Damian
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BACILLUS SAFENSIS
ANTAGONISM
PHYTOPATHOGENIC
XANTHOMONAS
topic BACILLUS SAFENSIS
ANTAGONISM
PHYTOPATHOGENIC
XANTHOMONAS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Citrus canker caused by Xanthomonas citri subsp. citri (Xcc), is a bacterial disease which affects all the citrics. One alternative to manage it is the use of antagonist bacteria. The aim of this work was to investigate the antagonist activity of Bacillus safensis (S9) against Xcc. The activity was tested by diffusion assays. Xcc and S9 were grown overnight in Luria Bertani (LB) and potato dextrose (PD) medium, respectively, with continuous agitation at 28°C and then, were diluted to a concentration of 108 CFU/mL. Petri dishes were covered with 15 mL of LB-agar containing 100 μL of the Xcc dilution. Once the medium was solidified, 4 μL drops of S9 were inoculated 3 times in each Petri dish, and the experiment was made by triplicate. After 48 hours of incubation at 28°C, the inhibition zone was measured, and the average inhibition area was calculated as IA = average area of the inhibition zone - average area of the colony. A significant inhibition area of 5.18 cm2 was obtained (one-sample t- test, p<0.05). At the same time, diffusion assays with the supernatant were made to prove its inhibitory ability. Petri dishes were prepared as described above. The supernatant was obtained by centrifugation of the S9 culture grown in PD medium, and then by bacteria filtration. Three filter paper discs embedded with the supernatant were placed per Petri dish, by triplicate. The inhibition zone was measured after 48 hours and calculated the IA. A significant inhibition area of 2.29 cm2 was obtained (one-sample t-test, p<0.05). Besides, a study at genomic level comparing S9 with ten Bacillus strains was made. Different clusters of secondary metabolite synthesis pathways were detected, three common with B. velezensis strains (surfactin, basilicin and bacilibactin). These strains were tested as inhibitors of Xcc and they did not show inhibition (Bacillus sp and B. megaterium) or showed less inhibition (B. velezensis). The difference might be on the expression level of the clusters. These results suggest the potential use of S9 as a canker control agent and further studies will be necessary to identify the Xcc- inhibitor metabolite.
Fil: Olivella, Laura. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias Agropecuarias del Litoral. - Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral.; Argentina
Fil: Gaido, Jimena Daiana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias Agropecuarias del Litoral. - Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral.; Argentina
Fil: Torres Manno, Mariano Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Orgánica; Argentina
Fil: Petitti, Tomás Denis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Espariz, Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Daurelio, Lucas Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology
Buenos Aires
Argentina
Sociedad Argentina de Bioquímica y Biología Molecular
Sociedad Argentina de Microbiología General
description Citrus canker caused by Xanthomonas citri subsp. citri (Xcc), is a bacterial disease which affects all the citrics. One alternative to manage it is the use of antagonist bacteria. The aim of this work was to investigate the antagonist activity of Bacillus safensis (S9) against Xcc. The activity was tested by diffusion assays. Xcc and S9 were grown overnight in Luria Bertani (LB) and potato dextrose (PD) medium, respectively, with continuous agitation at 28°C and then, were diluted to a concentration of 108 CFU/mL. Petri dishes were covered with 15 mL of LB-agar containing 100 μL of the Xcc dilution. Once the medium was solidified, 4 μL drops of S9 were inoculated 3 times in each Petri dish, and the experiment was made by triplicate. After 48 hours of incubation at 28°C, the inhibition zone was measured, and the average inhibition area was calculated as IA = average area of the inhibition zone - average area of the colony. A significant inhibition area of 5.18 cm2 was obtained (one-sample t- test, p<0.05). At the same time, diffusion assays with the supernatant were made to prove its inhibitory ability. Petri dishes were prepared as described above. The supernatant was obtained by centrifugation of the S9 culture grown in PD medium, and then by bacteria filtration. Three filter paper discs embedded with the supernatant were placed per Petri dish, by triplicate. The inhibition zone was measured after 48 hours and calculated the IA. A significant inhibition area of 2.29 cm2 was obtained (one-sample t-test, p<0.05). Besides, a study at genomic level comparing S9 with ten Bacillus strains was made. Different clusters of secondary metabolite synthesis pathways were detected, three common with B. velezensis strains (surfactin, basilicin and bacilibactin). These strains were tested as inhibitors of Xcc and they did not show inhibition (Bacillus sp and B. megaterium) or showed less inhibition (B. velezensis). The difference might be on the expression level of the clusters. These results suggest the potential use of S9 as a canker control agent and further studies will be necessary to identify the Xcc- inhibitor metabolite.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/211207
Antagonism of Bacillus safensis strain against phytopathogenic bacteria; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology; Buenos Aires; Argentina; 2021; 91-92
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/211207
identifier_str_mv Antagonism of Bacillus safensis strain against phytopathogenic bacteria; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology; Buenos Aires; Argentina; 2021; 91-92
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://congresos.g2consultora.com/wp-content/uploads/2021/10/Biocell-Preprint-SAIB-SAMIGE-2021.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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