Toll-like receptor 1 as a possible target in non-alcoholic fatty liver disease

Autores
Baumann, Anja; Nier, Anika; Hernández Arriaga, Angélica; Brandt, Annette; Lorenzo Pisarello, Maria Jose; Jin, Cheng J.; Pilar, Esther; Camarinha-Silva, Amélia; Schattenberg, Jörn M.; Bergheim, Ina
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Toll-like receptors (TLRs) in the liver compartment have repeatedly been attributed to the development of non-alcoholic fatty liver disease (NAFLD). Knowledge on TLR expression in blood cells and their relation to intestinal microbiota and NAFLD development is limited. Here, we determined TLR expression patterns in peripheral blood mononuclear cells (PBMCs) of NAFLD patients and controls, their relation to intestinal microbiota and the impact of TLRs found altered in NAFLD development. Markers of intestinal permeability in blood and TLR mRNA expression in PBMCs were determined in 37 NAFLD patients and 15 age-matched healthy controls. Fecal microbiota composition was evaluated in 21 NAFLD patients and 9 controls using 16S rRNA gene amplicon sequencing. Furthermore, TLR1−/− and C57BL/6 mice (n = 5–6/group) were pair-fed a liquid control or a fat-, fructose- and cholesterol-rich diet. Intestinal microbiota composition and markers of intestinal permeability like zonulin and bacterial endotoxin differed significantly between groups with the latter markers being significantly higher in NAFLD patients. Expression of TLR1-8 and 10 mRNA was detectable in PBMCs; however, only TLR1 expression, being higher in NAFLD patients, were significantly positively correlated with the prevalence of Holdemanella genus while negative correlations were found with Gemmiger and Ruminococcus genera. TLR1−/− mice were significantly protected from the development of diet-induced NAFLD when compared to wild-type mice. While intestinal microbiota composition and permeability differed significantly between NAFLD patients and healthy subjects, in PBMCs, only TLR1 expression differed between groups. Still, targeting these alterations might be a beneficial approach in the treatment of NAFLD in some patients.
Fil: Baumann, Anja. Universidad de Viena; Austria
Fil: Nier, Anika. Universidad de Viena; Austria
Fil: Hernández Arriaga, Angélica. Universidad de Hohenheim; Alemania
Fil: Brandt, Annette. Universidad de Viena; Austria
Fil: Lorenzo Pisarello, Maria Jose. Universidad de Viena; Austria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Jin, Cheng J.. Universitat Jena; Alemania
Fil: Pilar, Esther. Universidad de Viena; Austria
Fil: Camarinha-Silva, Amélia. Universidad de Hohenheim; Alemania
Fil: Schattenberg, Jörn M.. University Medical Center of the Johannes Gutenberg; Alemania
Fil: Bergheim, Ina. Universidad de Viena; Austria
Materia
TOLL-LIKE RECEPTORS
INTESTINAL MICROBIOTA
ENDOTOXIN
NASH
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/153582
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/153582
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Toll-like receptor 1 as a possible target in non-alcoholic fatty liver diseaseBaumann, AnjaNier, AnikaHernández Arriaga, AngélicaBrandt, AnnetteLorenzo Pisarello, Maria JoseJin, Cheng J.Pilar, EstherCamarinha-Silva, AméliaSchattenberg, Jörn M.Bergheim, InaTOLL-LIKE RECEPTORSINTESTINAL MICROBIOTAENDOTOXINNASHhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Toll-like receptors (TLRs) in the liver compartment have repeatedly been attributed to the development of non-alcoholic fatty liver disease (NAFLD). Knowledge on TLR expression in blood cells and their relation to intestinal microbiota and NAFLD development is limited. Here, we determined TLR expression patterns in peripheral blood mononuclear cells (PBMCs) of NAFLD patients and controls, their relation to intestinal microbiota and the impact of TLRs found altered in NAFLD development. Markers of intestinal permeability in blood and TLR mRNA expression in PBMCs were determined in 37 NAFLD patients and 15 age-matched healthy controls. Fecal microbiota composition was evaluated in 21 NAFLD patients and 9 controls using 16S rRNA gene amplicon sequencing. Furthermore, TLR1−/− and C57BL/6 mice (n = 5–6/group) were pair-fed a liquid control or a fat-, fructose- and cholesterol-rich diet. Intestinal microbiota composition and markers of intestinal permeability like zonulin and bacterial endotoxin differed significantly between groups with the latter markers being significantly higher in NAFLD patients. Expression of TLR1-8 and 10 mRNA was detectable in PBMCs; however, only TLR1 expression, being higher in NAFLD patients, were significantly positively correlated with the prevalence of Holdemanella genus while negative correlations were found with Gemmiger and Ruminococcus genera. TLR1−/− mice were significantly protected from the development of diet-induced NAFLD when compared to wild-type mice. While intestinal microbiota composition and permeability differed significantly between NAFLD patients and healthy subjects, in PBMCs, only TLR1 expression differed between groups. Still, targeting these alterations might be a beneficial approach in the treatment of NAFLD in some patients.Fil: Baumann, Anja. Universidad de Viena; AustriaFil: Nier, Anika. Universidad de Viena; AustriaFil: Hernández Arriaga, Angélica. Universidad de Hohenheim; AlemaniaFil: Brandt, Annette. Universidad de Viena; AustriaFil: Lorenzo Pisarello, Maria Jose. Universidad de Viena; Austria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Jin, Cheng J.. Universitat Jena; AlemaniaFil: Pilar, Esther. Universidad de Viena; AustriaFil: Camarinha-Silva, Amélia. Universidad de Hohenheim; AlemaniaFil: Schattenberg, Jörn M.. University Medical Center of the Johannes Gutenberg; AlemaniaFil: Bergheim, Ina. Universidad de Viena; AustriaNature Publishing Group2021-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/153582Baumann, Anja; Nier, Anika; Hernández Arriaga, Angélica; Brandt, Annette; Lorenzo Pisarello, Maria Jose; et al.; Toll-like receptor 1 as a possible target in non-alcoholic fatty liver disease; Nature Publishing Group; Scientific Reports; 11; 1; 9-2021; 1-15;178152045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-021-97346-9info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-021-97346-9info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426394/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:49Zoai:ri.conicet.gov.ar:11336/153582instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:49.449CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Toll-like receptor 1 as a possible target in non-alcoholic fatty liver disease
title Toll-like receptor 1 as a possible target in non-alcoholic fatty liver disease
spellingShingle Toll-like receptor 1 as a possible target in non-alcoholic fatty liver disease
Baumann, Anja
TOLL-LIKE RECEPTORS
INTESTINAL MICROBIOTA
ENDOTOXIN
NASH
title_short Toll-like receptor 1 as a possible target in non-alcoholic fatty liver disease
title_full Toll-like receptor 1 as a possible target in non-alcoholic fatty liver disease
title_fullStr Toll-like receptor 1 as a possible target in non-alcoholic fatty liver disease
title_full_unstemmed Toll-like receptor 1 as a possible target in non-alcoholic fatty liver disease
title_sort Toll-like receptor 1 as a possible target in non-alcoholic fatty liver disease
dc.creator.none.fl_str_mv Baumann, Anja
Nier, Anika
Hernández Arriaga, Angélica
Brandt, Annette
Lorenzo Pisarello, Maria Jose
Jin, Cheng J.
Pilar, Esther
Camarinha-Silva, Amélia
Schattenberg, Jörn M.
Bergheim, Ina
author Baumann, Anja
author_facet Baumann, Anja
Nier, Anika
Hernández Arriaga, Angélica
Brandt, Annette
Lorenzo Pisarello, Maria Jose
Jin, Cheng J.
Pilar, Esther
Camarinha-Silva, Amélia
Schattenberg, Jörn M.
Bergheim, Ina
author_role author
author2 Nier, Anika
Hernández Arriaga, Angélica
Brandt, Annette
Lorenzo Pisarello, Maria Jose
Jin, Cheng J.
Pilar, Esther
Camarinha-Silva, Amélia
Schattenberg, Jörn M.
Bergheim, Ina
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TOLL-LIKE RECEPTORS
INTESTINAL MICROBIOTA
ENDOTOXIN
NASH
topic TOLL-LIKE RECEPTORS
INTESTINAL MICROBIOTA
ENDOTOXIN
NASH
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Toll-like receptors (TLRs) in the liver compartment have repeatedly been attributed to the development of non-alcoholic fatty liver disease (NAFLD). Knowledge on TLR expression in blood cells and their relation to intestinal microbiota and NAFLD development is limited. Here, we determined TLR expression patterns in peripheral blood mononuclear cells (PBMCs) of NAFLD patients and controls, their relation to intestinal microbiota and the impact of TLRs found altered in NAFLD development. Markers of intestinal permeability in blood and TLR mRNA expression in PBMCs were determined in 37 NAFLD patients and 15 age-matched healthy controls. Fecal microbiota composition was evaluated in 21 NAFLD patients and 9 controls using 16S rRNA gene amplicon sequencing. Furthermore, TLR1−/− and C57BL/6 mice (n = 5–6/group) were pair-fed a liquid control or a fat-, fructose- and cholesterol-rich diet. Intestinal microbiota composition and markers of intestinal permeability like zonulin and bacterial endotoxin differed significantly between groups with the latter markers being significantly higher in NAFLD patients. Expression of TLR1-8 and 10 mRNA was detectable in PBMCs; however, only TLR1 expression, being higher in NAFLD patients, were significantly positively correlated with the prevalence of Holdemanella genus while negative correlations were found with Gemmiger and Ruminococcus genera. TLR1−/− mice were significantly protected from the development of diet-induced NAFLD when compared to wild-type mice. While intestinal microbiota composition and permeability differed significantly between NAFLD patients and healthy subjects, in PBMCs, only TLR1 expression differed between groups. Still, targeting these alterations might be a beneficial approach in the treatment of NAFLD in some patients.
Fil: Baumann, Anja. Universidad de Viena; Austria
Fil: Nier, Anika. Universidad de Viena; Austria
Fil: Hernández Arriaga, Angélica. Universidad de Hohenheim; Alemania
Fil: Brandt, Annette. Universidad de Viena; Austria
Fil: Lorenzo Pisarello, Maria Jose. Universidad de Viena; Austria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Jin, Cheng J.. Universitat Jena; Alemania
Fil: Pilar, Esther. Universidad de Viena; Austria
Fil: Camarinha-Silva, Amélia. Universidad de Hohenheim; Alemania
Fil: Schattenberg, Jörn M.. University Medical Center of the Johannes Gutenberg; Alemania
Fil: Bergheim, Ina. Universidad de Viena; Austria
description Toll-like receptors (TLRs) in the liver compartment have repeatedly been attributed to the development of non-alcoholic fatty liver disease (NAFLD). Knowledge on TLR expression in blood cells and their relation to intestinal microbiota and NAFLD development is limited. Here, we determined TLR expression patterns in peripheral blood mononuclear cells (PBMCs) of NAFLD patients and controls, their relation to intestinal microbiota and the impact of TLRs found altered in NAFLD development. Markers of intestinal permeability in blood and TLR mRNA expression in PBMCs were determined in 37 NAFLD patients and 15 age-matched healthy controls. Fecal microbiota composition was evaluated in 21 NAFLD patients and 9 controls using 16S rRNA gene amplicon sequencing. Furthermore, TLR1−/− and C57BL/6 mice (n = 5–6/group) were pair-fed a liquid control or a fat-, fructose- and cholesterol-rich diet. Intestinal microbiota composition and markers of intestinal permeability like zonulin and bacterial endotoxin differed significantly between groups with the latter markers being significantly higher in NAFLD patients. Expression of TLR1-8 and 10 mRNA was detectable in PBMCs; however, only TLR1 expression, being higher in NAFLD patients, were significantly positively correlated with the prevalence of Holdemanella genus while negative correlations were found with Gemmiger and Ruminococcus genera. TLR1−/− mice were significantly protected from the development of diet-induced NAFLD when compared to wild-type mice. While intestinal microbiota composition and permeability differed significantly between NAFLD patients and healthy subjects, in PBMCs, only TLR1 expression differed between groups. Still, targeting these alterations might be a beneficial approach in the treatment of NAFLD in some patients.
publishDate 2021
dc.date.none.fl_str_mv 2021-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/153582
Baumann, Anja; Nier, Anika; Hernández Arriaga, Angélica; Brandt, Annette; Lorenzo Pisarello, Maria Jose; et al.; Toll-like receptor 1 as a possible target in non-alcoholic fatty liver disease; Nature Publishing Group; Scientific Reports; 11; 1; 9-2021; 1-15;17815
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/153582
identifier_str_mv Baumann, Anja; Nier, Anika; Hernández Arriaga, Angélica; Brandt, Annette; Lorenzo Pisarello, Maria Jose; et al.; Toll-like receptor 1 as a possible target in non-alcoholic fatty liver disease; Nature Publishing Group; Scientific Reports; 11; 1; 9-2021; 1-15;17815
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-021-97346-9
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-021-97346-9
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426394/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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