Development of cecal-predominant microbiota in broilers during a complete rearing using denaturing gradient gel electrophoresis

Autores
Blajman, Jesica; Zbrun, María Virginia; Signorini Porchietto, Marcelo Lisandro; Zimmermann, Jorge Alberto; Rossler, Eugenia; Berisvil, Ayelén Patricia; Romero Scharpen, Analía; Astesana, Diego Martín; Soto, Lorena Paola; Frizzo, Laureano Sebastian
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Understanding of the intestinal microbiota is crucial to enhance intestinal health and performance parameters in animals. A more exhaustive research of the intestinal microbiota of broilers could be of interest to implement appropriate intervention measures. The aim of the present study was to investigate the development of the predominant cecal microbiota in broilers that were fed a Lactobacillus salivarius DSPV 001P strain during a complete rearing using denaturing gradient gel electrophoresis (DGGE). Bacterial DNA from cecal samples of 24 broilers at different ages were amplified by PCR and analysed by DGGE. A total of 35 DGGE products were excised and sequenced. Distinctive differences in bacterial communities were observed in the caecum as broilers age. At early stages, identified bacteria within the caecum of broilers were predominantly Clostridium-related species. Also, some sequences had the closest match to the genus Escherichia/Shigella. Furthermore, the caecum was a reservoir rich in uncultured bacteria. The major difference observed in our study was an increase of potentially beneficial Lactobacillus at Day 45. These results may be attributed to modulation of the microbiota by the probiotic supplementation. The obtained data could be relevant for future studies related to the influence of the microbiota resulting from probiotic supplementation on the performance and the immunological parameters of broilers.
Fil: Blajman, Jesica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Zbrun, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Signorini Porchietto, Marcelo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Zimmermann, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Rossler, Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Berisvil, Ayelén Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Romero Scharpen, Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Astesana, Diego Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Soto, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Frizzo, Laureano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Materia
Bacterial Communities
Intestinal Health
Lactobacillus Salivarius
Probiotic
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/64084
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of cecal-predominant microbiota in broilers during a complete rearing using denaturing gradient gel electrophoresisBlajman, JesicaZbrun, María VirginiaSignorini Porchietto, Marcelo LisandroZimmermann, Jorge AlbertoRossler, EugeniaBerisvil, Ayelén PatriciaRomero Scharpen, AnalíaAstesana, Diego MartínSoto, Lorena PaolaFrizzo, Laureano SebastianBacterial CommunitiesIntestinal HealthLactobacillus SalivariusProbiotichttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Understanding of the intestinal microbiota is crucial to enhance intestinal health and performance parameters in animals. A more exhaustive research of the intestinal microbiota of broilers could be of interest to implement appropriate intervention measures. The aim of the present study was to investigate the development of the predominant cecal microbiota in broilers that were fed a Lactobacillus salivarius DSPV 001P strain during a complete rearing using denaturing gradient gel electrophoresis (DGGE). Bacterial DNA from cecal samples of 24 broilers at different ages were amplified by PCR and analysed by DGGE. A total of 35 DGGE products were excised and sequenced. Distinctive differences in bacterial communities were observed in the caecum as broilers age. At early stages, identified bacteria within the caecum of broilers were predominantly Clostridium-related species. Also, some sequences had the closest match to the genus Escherichia/Shigella. Furthermore, the caecum was a reservoir rich in uncultured bacteria. The major difference observed in our study was an increase of potentially beneficial Lactobacillus at Day 45. These results may be attributed to modulation of the microbiota by the probiotic supplementation. The obtained data could be relevant for future studies related to the influence of the microbiota resulting from probiotic supplementation on the performance and the immunological parameters of broilers.Fil: Blajman, Jesica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Zbrun, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Signorini Porchietto, Marcelo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Zimmermann, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Rossler, Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Berisvil, Ayelén Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Romero Scharpen, Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Astesana, Diego Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Soto, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Frizzo, Laureano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaCsiro Publishing2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64084Blajman, Jesica; Zbrun, María Virginia; Signorini Porchietto, Marcelo Lisandro; Zimmermann, Jorge Alberto; Rossler, Eugenia; et al.; Development of cecal-predominant microbiota in broilers during a complete rearing using denaturing gradient gel electrophoresis; Csiro Publishing; Animal Production Science; 57; 3; 11-2017; 458-4651836-5787CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.publish.csiro.au/paper/AN15475.htminfo:eu-repo/semantics/altIdentifier/doi/10.1071/AN15475info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:48Zoai:ri.conicet.gov.ar:11336/64084instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:48.882CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of cecal-predominant microbiota in broilers during a complete rearing using denaturing gradient gel electrophoresis
title Development of cecal-predominant microbiota in broilers during a complete rearing using denaturing gradient gel electrophoresis
spellingShingle Development of cecal-predominant microbiota in broilers during a complete rearing using denaturing gradient gel electrophoresis
Blajman, Jesica
Bacterial Communities
Intestinal Health
Lactobacillus Salivarius
Probiotic
title_short Development of cecal-predominant microbiota in broilers during a complete rearing using denaturing gradient gel electrophoresis
title_full Development of cecal-predominant microbiota in broilers during a complete rearing using denaturing gradient gel electrophoresis
title_fullStr Development of cecal-predominant microbiota in broilers during a complete rearing using denaturing gradient gel electrophoresis
title_full_unstemmed Development of cecal-predominant microbiota in broilers during a complete rearing using denaturing gradient gel electrophoresis
title_sort Development of cecal-predominant microbiota in broilers during a complete rearing using denaturing gradient gel electrophoresis
dc.creator.none.fl_str_mv Blajman, Jesica
Zbrun, María Virginia
Signorini Porchietto, Marcelo Lisandro
Zimmermann, Jorge Alberto
Rossler, Eugenia
Berisvil, Ayelén Patricia
Romero Scharpen, Analía
Astesana, Diego Martín
Soto, Lorena Paola
Frizzo, Laureano Sebastian
author Blajman, Jesica
author_facet Blajman, Jesica
Zbrun, María Virginia
Signorini Porchietto, Marcelo Lisandro
Zimmermann, Jorge Alberto
Rossler, Eugenia
Berisvil, Ayelén Patricia
Romero Scharpen, Analía
Astesana, Diego Martín
Soto, Lorena Paola
Frizzo, Laureano Sebastian
author_role author
author2 Zbrun, María Virginia
Signorini Porchietto, Marcelo Lisandro
Zimmermann, Jorge Alberto
Rossler, Eugenia
Berisvil, Ayelén Patricia
Romero Scharpen, Analía
Astesana, Diego Martín
Soto, Lorena Paola
Frizzo, Laureano Sebastian
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bacterial Communities
Intestinal Health
Lactobacillus Salivarius
Probiotic
topic Bacterial Communities
Intestinal Health
Lactobacillus Salivarius
Probiotic
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Understanding of the intestinal microbiota is crucial to enhance intestinal health and performance parameters in animals. A more exhaustive research of the intestinal microbiota of broilers could be of interest to implement appropriate intervention measures. The aim of the present study was to investigate the development of the predominant cecal microbiota in broilers that were fed a Lactobacillus salivarius DSPV 001P strain during a complete rearing using denaturing gradient gel electrophoresis (DGGE). Bacterial DNA from cecal samples of 24 broilers at different ages were amplified by PCR and analysed by DGGE. A total of 35 DGGE products were excised and sequenced. Distinctive differences in bacterial communities were observed in the caecum as broilers age. At early stages, identified bacteria within the caecum of broilers were predominantly Clostridium-related species. Also, some sequences had the closest match to the genus Escherichia/Shigella. Furthermore, the caecum was a reservoir rich in uncultured bacteria. The major difference observed in our study was an increase of potentially beneficial Lactobacillus at Day 45. These results may be attributed to modulation of the microbiota by the probiotic supplementation. The obtained data could be relevant for future studies related to the influence of the microbiota resulting from probiotic supplementation on the performance and the immunological parameters of broilers.
Fil: Blajman, Jesica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Zbrun, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Signorini Porchietto, Marcelo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Zimmermann, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Rossler, Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Berisvil, Ayelén Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Romero Scharpen, Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Astesana, Diego Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Soto, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Frizzo, Laureano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
description Understanding of the intestinal microbiota is crucial to enhance intestinal health and performance parameters in animals. A more exhaustive research of the intestinal microbiota of broilers could be of interest to implement appropriate intervention measures. The aim of the present study was to investigate the development of the predominant cecal microbiota in broilers that were fed a Lactobacillus salivarius DSPV 001P strain during a complete rearing using denaturing gradient gel electrophoresis (DGGE). Bacterial DNA from cecal samples of 24 broilers at different ages were amplified by PCR and analysed by DGGE. A total of 35 DGGE products were excised and sequenced. Distinctive differences in bacterial communities were observed in the caecum as broilers age. At early stages, identified bacteria within the caecum of broilers were predominantly Clostridium-related species. Also, some sequences had the closest match to the genus Escherichia/Shigella. Furthermore, the caecum was a reservoir rich in uncultured bacteria. The major difference observed in our study was an increase of potentially beneficial Lactobacillus at Day 45. These results may be attributed to modulation of the microbiota by the probiotic supplementation. The obtained data could be relevant for future studies related to the influence of the microbiota resulting from probiotic supplementation on the performance and the immunological parameters of broilers.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/64084
Blajman, Jesica; Zbrun, María Virginia; Signorini Porchietto, Marcelo Lisandro; Zimmermann, Jorge Alberto; Rossler, Eugenia; et al.; Development of cecal-predominant microbiota in broilers during a complete rearing using denaturing gradient gel electrophoresis; Csiro Publishing; Animal Production Science; 57; 3; 11-2017; 458-465
1836-5787
CONICET Digital
CONICET
url http://hdl.handle.net/11336/64084
identifier_str_mv Blajman, Jesica; Zbrun, María Virginia; Signorini Porchietto, Marcelo Lisandro; Zimmermann, Jorge Alberto; Rossler, Eugenia; et al.; Development of cecal-predominant microbiota in broilers during a complete rearing using denaturing gradient gel electrophoresis; Csiro Publishing; Animal Production Science; 57; 3; 11-2017; 458-465
1836-5787
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.publish.csiro.au/paper/AN15475.htm
info:eu-repo/semantics/altIdentifier/doi/10.1071/AN15475
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Csiro Publishing
publisher.none.fl_str_mv Csiro Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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