PrbP modulates biofilm formation in Liberibacter crescens

Autores
Pan, Lei; Gardner, Christopher L.; Beliakoff, Reagan; Da Silva, Danilo; Zuo, Ran; Pagliai, Fernando A.; Padgett Pagliai, Kaylie A.; Merli, Marcelo Luciano; Bahadiroglu, Erol; Gonzalez, Claudio F.; Lorca, Graciela L.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Liberibacter asiaticus, PrbP is a transcriptional regulatory protein involved in survival and persistence during host infection. Tolfenamic acid was previously found to inhibit interactions between PrbP and the promotor region of rplK, resulting in reduced survival of L. asiaticus in the citrus host. In this study, we performed transcriptome analyses to elucidate the PrbP regulon in L. crescens, as it is phylogenetically the closest related species to L. asiaticus that can be grown in laboratory conditions. Chemical inhibition of PrbP with tolfenamic acid revealed that PrbP is involved in the regulation of diverse cellular processes, including stress response, cell motility, cell cycle and biofilm formation. In vitro DNA binding and bacterial two-hybrid assays also suggested that PrbP is a global regulator of multiple transcription factors (RpoH, VisN, PleD, MucR, MocR and CtrA) at both transcriptional and/or post-transcriptional levels. Sub-lethal concentrations of tolfenamic acid significantly reduced the attachment of L. crescens during biofilm formation and decreased long-term persistence in biofilm structures. Overall, our findings show the importance of PrbP in regulating diverse biological processes through direct and indirect interactions with other transcriptional regulators in L. crescens.
Fil: Pan, Lei. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Gardner, Christopher L.. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Beliakoff, Reagan. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Da Silva, Danilo. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Zuo, Ran. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Pagliai, Fernando A.. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Padgett Pagliai, Kaylie A.. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Merli, Marcelo Luciano. University of Florida. Department of Microbiology and Cell Science; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Bahadiroglu, Erol. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Gonzalez, Claudio F.. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Lorca, Graciela L.. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Materia
PrbP
transcriptional accessory protein
Liberibacter crescens
biofilm
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/182553
Acceder
id CONICETDig_c317a816fc768dcc7c1fa3448cb91a3d
oai_identifier_str oai:ri.conicet.gov.ar:11336/182553
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling PrbP modulates biofilm formation in Liberibacter crescensPan, LeiGardner, Christopher L.Beliakoff, ReaganDa Silva, DaniloZuo, RanPagliai, Fernando A.Padgett Pagliai, Kaylie A.Merli, Marcelo LucianoBahadiroglu, ErolGonzalez, Claudio F.Lorca, Graciela L.PrbPtranscriptional accessory proteinLiberibacter crescensbiofilmhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In Liberibacter asiaticus, PrbP is a transcriptional regulatory protein involved in survival and persistence during host infection. Tolfenamic acid was previously found to inhibit interactions between PrbP and the promotor region of rplK, resulting in reduced survival of L. asiaticus in the citrus host. In this study, we performed transcriptome analyses to elucidate the PrbP regulon in L. crescens, as it is phylogenetically the closest related species to L. asiaticus that can be grown in laboratory conditions. Chemical inhibition of PrbP with tolfenamic acid revealed that PrbP is involved in the regulation of diverse cellular processes, including stress response, cell motility, cell cycle and biofilm formation. In vitro DNA binding and bacterial two-hybrid assays also suggested that PrbP is a global regulator of multiple transcription factors (RpoH, VisN, PleD, MucR, MocR and CtrA) at both transcriptional and/or post-transcriptional levels. Sub-lethal concentrations of tolfenamic acid significantly reduced the attachment of L. crescens during biofilm formation and decreased long-term persistence in biofilm structures. Overall, our findings show the importance of PrbP in regulating diverse biological processes through direct and indirect interactions with other transcriptional regulators in L. crescens.Fil: Pan, Lei. University of Florida. Department of Microbiology and Cell Science; Estados UnidosFil: Gardner, Christopher L.. University of Florida. Department of Microbiology and Cell Science; Estados UnidosFil: Beliakoff, Reagan. University of Florida. Department of Microbiology and Cell Science; Estados UnidosFil: Da Silva, Danilo. University of Florida. Department of Microbiology and Cell Science; Estados UnidosFil: Zuo, Ran. University of Florida. Department of Microbiology and Cell Science; Estados UnidosFil: Pagliai, Fernando A.. University of Florida. Department of Microbiology and Cell Science; Estados UnidosFil: Padgett Pagliai, Kaylie A.. University of Florida. Department of Microbiology and Cell Science; Estados UnidosFil: Merli, Marcelo Luciano. University of Florida. Department of Microbiology and Cell Science; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Bahadiroglu, Erol. University of Florida. Department of Microbiology and Cell Science; Estados UnidosFil: Gonzalez, Claudio F.. University of Florida. Department of Microbiology and Cell Science; Estados UnidosFil: Lorca, Graciela L.. University of Florida. Department of Microbiology and Cell Science; Estados UnidosWiley Blackwell Publishing, Inc2021-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/182553Pan, Lei; Gardner, Christopher L.; Beliakoff, Reagan; Da Silva, Danilo; Zuo, Ran; et al.; PrbP modulates biofilm formation in Liberibacter crescens; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 23; 11; 8-2021; 7121-71381462-2912CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/1462-2920.15740info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.15740info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:13Zoai:ri.conicet.gov.ar:11336/182553instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:14.101CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv PrbP modulates biofilm formation in Liberibacter crescens
title PrbP modulates biofilm formation in Liberibacter crescens
spellingShingle PrbP modulates biofilm formation in Liberibacter crescens
Pan, Lei
PrbP
transcriptional accessory protein
Liberibacter crescens
biofilm
title_short PrbP modulates biofilm formation in Liberibacter crescens
title_full PrbP modulates biofilm formation in Liberibacter crescens
title_fullStr PrbP modulates biofilm formation in Liberibacter crescens
title_full_unstemmed PrbP modulates biofilm formation in Liberibacter crescens
title_sort PrbP modulates biofilm formation in Liberibacter crescens
dc.creator.none.fl_str_mv Pan, Lei
Gardner, Christopher L.
Beliakoff, Reagan
Da Silva, Danilo
Zuo, Ran
Pagliai, Fernando A.
Padgett Pagliai, Kaylie A.
Merli, Marcelo Luciano
Bahadiroglu, Erol
Gonzalez, Claudio F.
Lorca, Graciela L.
author Pan, Lei
author_facet Pan, Lei
Gardner, Christopher L.
Beliakoff, Reagan
Da Silva, Danilo
Zuo, Ran
Pagliai, Fernando A.
Padgett Pagliai, Kaylie A.
Merli, Marcelo Luciano
Bahadiroglu, Erol
Gonzalez, Claudio F.
Lorca, Graciela L.
author_role author
author2 Gardner, Christopher L.
Beliakoff, Reagan
Da Silva, Danilo
Zuo, Ran
Pagliai, Fernando A.
Padgett Pagliai, Kaylie A.
Merli, Marcelo Luciano
Bahadiroglu, Erol
Gonzalez, Claudio F.
Lorca, Graciela L.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PrbP
transcriptional accessory protein
Liberibacter crescens
biofilm
topic PrbP
transcriptional accessory protein
Liberibacter crescens
biofilm
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In Liberibacter asiaticus, PrbP is a transcriptional regulatory protein involved in survival and persistence during host infection. Tolfenamic acid was previously found to inhibit interactions between PrbP and the promotor region of rplK, resulting in reduced survival of L. asiaticus in the citrus host. In this study, we performed transcriptome analyses to elucidate the PrbP regulon in L. crescens, as it is phylogenetically the closest related species to L. asiaticus that can be grown in laboratory conditions. Chemical inhibition of PrbP with tolfenamic acid revealed that PrbP is involved in the regulation of diverse cellular processes, including stress response, cell motility, cell cycle and biofilm formation. In vitro DNA binding and bacterial two-hybrid assays also suggested that PrbP is a global regulator of multiple transcription factors (RpoH, VisN, PleD, MucR, MocR and CtrA) at both transcriptional and/or post-transcriptional levels. Sub-lethal concentrations of tolfenamic acid significantly reduced the attachment of L. crescens during biofilm formation and decreased long-term persistence in biofilm structures. Overall, our findings show the importance of PrbP in regulating diverse biological processes through direct and indirect interactions with other transcriptional regulators in L. crescens.
Fil: Pan, Lei. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Gardner, Christopher L.. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Beliakoff, Reagan. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Da Silva, Danilo. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Zuo, Ran. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Pagliai, Fernando A.. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Padgett Pagliai, Kaylie A.. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Merli, Marcelo Luciano. University of Florida. Department of Microbiology and Cell Science; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Bahadiroglu, Erol. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Gonzalez, Claudio F.. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
Fil: Lorca, Graciela L.. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
description In Liberibacter asiaticus, PrbP is a transcriptional regulatory protein involved in survival and persistence during host infection. Tolfenamic acid was previously found to inhibit interactions between PrbP and the promotor region of rplK, resulting in reduced survival of L. asiaticus in the citrus host. In this study, we performed transcriptome analyses to elucidate the PrbP regulon in L. crescens, as it is phylogenetically the closest related species to L. asiaticus that can be grown in laboratory conditions. Chemical inhibition of PrbP with tolfenamic acid revealed that PrbP is involved in the regulation of diverse cellular processes, including stress response, cell motility, cell cycle and biofilm formation. In vitro DNA binding and bacterial two-hybrid assays also suggested that PrbP is a global regulator of multiple transcription factors (RpoH, VisN, PleD, MucR, MocR and CtrA) at both transcriptional and/or post-transcriptional levels. Sub-lethal concentrations of tolfenamic acid significantly reduced the attachment of L. crescens during biofilm formation and decreased long-term persistence in biofilm structures. Overall, our findings show the importance of PrbP in regulating diverse biological processes through direct and indirect interactions with other transcriptional regulators in L. crescens.
publishDate 2021
dc.date.none.fl_str_mv 2021-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/182553
Pan, Lei; Gardner, Christopher L.; Beliakoff, Reagan; Da Silva, Danilo; Zuo, Ran; et al.; PrbP modulates biofilm formation in Liberibacter crescens; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 23; 11; 8-2021; 7121-7138
1462-2912
CONICET Digital
CONICET
url http://hdl.handle.net/11336/182553
identifier_str_mv Pan, Lei; Gardner, Christopher L.; Beliakoff, Reagan; Da Silva, Danilo; Zuo, Ran; et al.; PrbP modulates biofilm formation in Liberibacter crescens; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 23; 11; 8-2021; 7121-7138
1462-2912
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/1462-2920.15740
info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.15740
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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