Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization

Autores
Muro, Yuko; Buffone, Mariano Gabriel; Okabe, Masaru; Gerton, George L.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In mammalian fertilization, sperm-zona pellucida binding is considered to be a critical aspect of gamete interaction. In this study, we examine the mouse sperm acrosomal matrix protein zona pellucida 3 receptor (ZP3R; formerly called sp56) because of our interest in defining the function of the acrosomal matrix, the particulate compartment within the sperm secretory acrosome. Using targeted deletion of the Zp3r gene by homologous recombination, we examined the fertility of nullizygous animals. Our experiments showed that males and females homozygous for the affected gene exhibited no differences in litter sizes compared to wild-type and heterozygous animals. Testis weights of nullizygous males were equivalent to those of wild-type and heterozygous males, and no differences in the number of sperm produced by mice of three genotypes were found. In vitro fertilization rates using cumulus-intact and cumulus-free oocytes were also equivalent. Examination of sperm-binding zonae of unfertilized eggs and the ability of the sperm to undergo acrosomal exocytosis in response to calcium ionophore A23187 displayed no differences between wild-type, heterozygous, and nullizygous mouse sperm. These results provide further evidence that either ZP3R is not involved in sperm-zona pellucida binding or this process might be functionally redundant, involving multiple proteins for gamete interactions.
Fil: Muro, Yuko. Osaka University; Japón
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Okabe, Masaru. Osaka University; Japón
Fil: Gerton, George L.. University of Pennsylvania; Estados Unidos
Materia
Sp56
Sperm
Zona Pellucida
Fertilization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/9857

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network_name_str CONICET Digital (CONICET)
spelling Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilizationMuro, YukoBuffone, Mariano GabrielOkabe, MasaruGerton, George L.Sp56SpermZona PellucidaFertilizationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In mammalian fertilization, sperm-zona pellucida binding is considered to be a critical aspect of gamete interaction. In this study, we examine the mouse sperm acrosomal matrix protein zona pellucida 3 receptor (ZP3R; formerly called sp56) because of our interest in defining the function of the acrosomal matrix, the particulate compartment within the sperm secretory acrosome. Using targeted deletion of the Zp3r gene by homologous recombination, we examined the fertility of nullizygous animals. Our experiments showed that males and females homozygous for the affected gene exhibited no differences in litter sizes compared to wild-type and heterozygous animals. Testis weights of nullizygous males were equivalent to those of wild-type and heterozygous males, and no differences in the number of sperm produced by mice of three genotypes were found. In vitro fertilization rates using cumulus-intact and cumulus-free oocytes were also equivalent. Examination of sperm-binding zonae of unfertilized eggs and the ability of the sperm to undergo acrosomal exocytosis in response to calcium ionophore A23187 displayed no differences between wild-type, heterozygous, and nullizygous mouse sperm. These results provide further evidence that either ZP3R is not involved in sperm-zona pellucida binding or this process might be functionally redundant, involving multiple proteins for gamete interactions.Fil: Muro, Yuko. Osaka University; JapónFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Okabe, Masaru. Osaka University; JapónFil: Gerton, George L.. University of Pennsylvania; Estados UnidosSociety For The Study Of Reproduction2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/9857Muro, Yuko; Buffone, Mariano Gabriel; Okabe, Masaru; Gerton, George L.; Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization; Society For The Study Of Reproduction; Biology Of Reproduction; 86; 1; 1-2012; 1-60006-33631529-7268enginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/86/1/23,%201-6/2530636info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.111.095877info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:04Zoai:ri.conicet.gov.ar:11336/9857instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:04.568CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization
title Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization
spellingShingle Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization
Muro, Yuko
Sp56
Sperm
Zona Pellucida
Fertilization
title_short Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization
title_full Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization
title_fullStr Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization
title_full_unstemmed Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization
title_sort Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization
dc.creator.none.fl_str_mv Muro, Yuko
Buffone, Mariano Gabriel
Okabe, Masaru
Gerton, George L.
author Muro, Yuko
author_facet Muro, Yuko
Buffone, Mariano Gabriel
Okabe, Masaru
Gerton, George L.
author_role author
author2 Buffone, Mariano Gabriel
Okabe, Masaru
Gerton, George L.
author2_role author
author
author
dc.subject.none.fl_str_mv Sp56
Sperm
Zona Pellucida
Fertilization
topic Sp56
Sperm
Zona Pellucida
Fertilization
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In mammalian fertilization, sperm-zona pellucida binding is considered to be a critical aspect of gamete interaction. In this study, we examine the mouse sperm acrosomal matrix protein zona pellucida 3 receptor (ZP3R; formerly called sp56) because of our interest in defining the function of the acrosomal matrix, the particulate compartment within the sperm secretory acrosome. Using targeted deletion of the Zp3r gene by homologous recombination, we examined the fertility of nullizygous animals. Our experiments showed that males and females homozygous for the affected gene exhibited no differences in litter sizes compared to wild-type and heterozygous animals. Testis weights of nullizygous males were equivalent to those of wild-type and heterozygous males, and no differences in the number of sperm produced by mice of three genotypes were found. In vitro fertilization rates using cumulus-intact and cumulus-free oocytes were also equivalent. Examination of sperm-binding zonae of unfertilized eggs and the ability of the sperm to undergo acrosomal exocytosis in response to calcium ionophore A23187 displayed no differences between wild-type, heterozygous, and nullizygous mouse sperm. These results provide further evidence that either ZP3R is not involved in sperm-zona pellucida binding or this process might be functionally redundant, involving multiple proteins for gamete interactions.
Fil: Muro, Yuko. Osaka University; Japón
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Okabe, Masaru. Osaka University; Japón
Fil: Gerton, George L.. University of Pennsylvania; Estados Unidos
description In mammalian fertilization, sperm-zona pellucida binding is considered to be a critical aspect of gamete interaction. In this study, we examine the mouse sperm acrosomal matrix protein zona pellucida 3 receptor (ZP3R; formerly called sp56) because of our interest in defining the function of the acrosomal matrix, the particulate compartment within the sperm secretory acrosome. Using targeted deletion of the Zp3r gene by homologous recombination, we examined the fertility of nullizygous animals. Our experiments showed that males and females homozygous for the affected gene exhibited no differences in litter sizes compared to wild-type and heterozygous animals. Testis weights of nullizygous males were equivalent to those of wild-type and heterozygous males, and no differences in the number of sperm produced by mice of three genotypes were found. In vitro fertilization rates using cumulus-intact and cumulus-free oocytes were also equivalent. Examination of sperm-binding zonae of unfertilized eggs and the ability of the sperm to undergo acrosomal exocytosis in response to calcium ionophore A23187 displayed no differences between wild-type, heterozygous, and nullizygous mouse sperm. These results provide further evidence that either ZP3R is not involved in sperm-zona pellucida binding or this process might be functionally redundant, involving multiple proteins for gamete interactions.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/9857
Muro, Yuko; Buffone, Mariano Gabriel; Okabe, Masaru; Gerton, George L.; Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization; Society For The Study Of Reproduction; Biology Of Reproduction; 86; 1; 1-2012; 1-6
0006-3363
1529-7268
url http://hdl.handle.net/11336/9857
identifier_str_mv Muro, Yuko; Buffone, Mariano Gabriel; Okabe, Masaru; Gerton, George L.; Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization; Society For The Study Of Reproduction; Biology Of Reproduction; 86; 1; 1-2012; 1-6
0006-3363
1529-7268
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/86/1/23,%201-6/2530636
info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.111.095877
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.publisher.none.fl_str_mv Society For The Study Of Reproduction
publisher.none.fl_str_mv Society For The Study Of Reproduction
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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