Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization
- Autores
- Muro, Yuko; Buffone, Mariano Gabriel; Okabe, Masaru; Gerton, George L.
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In mammalian fertilization, sperm-zona pellucida binding is considered to be a critical aspect of gamete interaction. In this study, we examine the mouse sperm acrosomal matrix protein zona pellucida 3 receptor (ZP3R; formerly called sp56) because of our interest in defining the function of the acrosomal matrix, the particulate compartment within the sperm secretory acrosome. Using targeted deletion of the Zp3r gene by homologous recombination, we examined the fertility of nullizygous animals. Our experiments showed that males and females homozygous for the affected gene exhibited no differences in litter sizes compared to wild-type and heterozygous animals. Testis weights of nullizygous males were equivalent to those of wild-type and heterozygous males, and no differences in the number of sperm produced by mice of three genotypes were found. In vitro fertilization rates using cumulus-intact and cumulus-free oocytes were also equivalent. Examination of sperm-binding zonae of unfertilized eggs and the ability of the sperm to undergo acrosomal exocytosis in response to calcium ionophore A23187 displayed no differences between wild-type, heterozygous, and nullizygous mouse sperm. These results provide further evidence that either ZP3R is not involved in sperm-zona pellucida binding or this process might be functionally redundant, involving multiple proteins for gamete interactions.
Fil: Muro, Yuko. Osaka University; Japón
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Okabe, Masaru. Osaka University; Japón
Fil: Gerton, George L.. University of Pennsylvania; Estados Unidos - Materia
-
Sp56
Sperm
Zona Pellucida
Fertilization - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/9857
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Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilizationMuro, YukoBuffone, Mariano GabrielOkabe, MasaruGerton, George L.Sp56SpermZona PellucidaFertilizationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In mammalian fertilization, sperm-zona pellucida binding is considered to be a critical aspect of gamete interaction. In this study, we examine the mouse sperm acrosomal matrix protein zona pellucida 3 receptor (ZP3R; formerly called sp56) because of our interest in defining the function of the acrosomal matrix, the particulate compartment within the sperm secretory acrosome. Using targeted deletion of the Zp3r gene by homologous recombination, we examined the fertility of nullizygous animals. Our experiments showed that males and females homozygous for the affected gene exhibited no differences in litter sizes compared to wild-type and heterozygous animals. Testis weights of nullizygous males were equivalent to those of wild-type and heterozygous males, and no differences in the number of sperm produced by mice of three genotypes were found. In vitro fertilization rates using cumulus-intact and cumulus-free oocytes were also equivalent. Examination of sperm-binding zonae of unfertilized eggs and the ability of the sperm to undergo acrosomal exocytosis in response to calcium ionophore A23187 displayed no differences between wild-type, heterozygous, and nullizygous mouse sperm. These results provide further evidence that either ZP3R is not involved in sperm-zona pellucida binding or this process might be functionally redundant, involving multiple proteins for gamete interactions.Fil: Muro, Yuko. Osaka University; JapónFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Okabe, Masaru. Osaka University; JapónFil: Gerton, George L.. University of Pennsylvania; Estados UnidosSociety For The Study Of Reproduction2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/9857Muro, Yuko; Buffone, Mariano Gabriel; Okabe, Masaru; Gerton, George L.; Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization; Society For The Study Of Reproduction; Biology Of Reproduction; 86; 1; 1-2012; 1-60006-33631529-7268enginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/86/1/23,%201-6/2530636info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.111.095877info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:04Zoai:ri.conicet.gov.ar:11336/9857instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:04.568CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization |
title |
Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization |
spellingShingle |
Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization Muro, Yuko Sp56 Sperm Zona Pellucida Fertilization |
title_short |
Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization |
title_full |
Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization |
title_fullStr |
Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization |
title_full_unstemmed |
Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization |
title_sort |
Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization |
dc.creator.none.fl_str_mv |
Muro, Yuko Buffone, Mariano Gabriel Okabe, Masaru Gerton, George L. |
author |
Muro, Yuko |
author_facet |
Muro, Yuko Buffone, Mariano Gabriel Okabe, Masaru Gerton, George L. |
author_role |
author |
author2 |
Buffone, Mariano Gabriel Okabe, Masaru Gerton, George L. |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Sp56 Sperm Zona Pellucida Fertilization |
topic |
Sp56 Sperm Zona Pellucida Fertilization |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In mammalian fertilization, sperm-zona pellucida binding is considered to be a critical aspect of gamete interaction. In this study, we examine the mouse sperm acrosomal matrix protein zona pellucida 3 receptor (ZP3R; formerly called sp56) because of our interest in defining the function of the acrosomal matrix, the particulate compartment within the sperm secretory acrosome. Using targeted deletion of the Zp3r gene by homologous recombination, we examined the fertility of nullizygous animals. Our experiments showed that males and females homozygous for the affected gene exhibited no differences in litter sizes compared to wild-type and heterozygous animals. Testis weights of nullizygous males were equivalent to those of wild-type and heterozygous males, and no differences in the number of sperm produced by mice of three genotypes were found. In vitro fertilization rates using cumulus-intact and cumulus-free oocytes were also equivalent. Examination of sperm-binding zonae of unfertilized eggs and the ability of the sperm to undergo acrosomal exocytosis in response to calcium ionophore A23187 displayed no differences between wild-type, heterozygous, and nullizygous mouse sperm. These results provide further evidence that either ZP3R is not involved in sperm-zona pellucida binding or this process might be functionally redundant, involving multiple proteins for gamete interactions. Fil: Muro, Yuko. Osaka University; Japón Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina Fil: Okabe, Masaru. Osaka University; Japón Fil: Gerton, George L.. University of Pennsylvania; Estados Unidos |
description |
In mammalian fertilization, sperm-zona pellucida binding is considered to be a critical aspect of gamete interaction. In this study, we examine the mouse sperm acrosomal matrix protein zona pellucida 3 receptor (ZP3R; formerly called sp56) because of our interest in defining the function of the acrosomal matrix, the particulate compartment within the sperm secretory acrosome. Using targeted deletion of the Zp3r gene by homologous recombination, we examined the fertility of nullizygous animals. Our experiments showed that males and females homozygous for the affected gene exhibited no differences in litter sizes compared to wild-type and heterozygous animals. Testis weights of nullizygous males were equivalent to those of wild-type and heterozygous males, and no differences in the number of sperm produced by mice of three genotypes were found. In vitro fertilization rates using cumulus-intact and cumulus-free oocytes were also equivalent. Examination of sperm-binding zonae of unfertilized eggs and the ability of the sperm to undergo acrosomal exocytosis in response to calcium ionophore A23187 displayed no differences between wild-type, heterozygous, and nullizygous mouse sperm. These results provide further evidence that either ZP3R is not involved in sperm-zona pellucida binding or this process might be functionally redundant, involving multiple proteins for gamete interactions. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/9857 Muro, Yuko; Buffone, Mariano Gabriel; Okabe, Masaru; Gerton, George L.; Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization; Society For The Study Of Reproduction; Biology Of Reproduction; 86; 1; 1-2012; 1-6 0006-3363 1529-7268 |
url |
http://hdl.handle.net/11336/9857 |
identifier_str_mv |
Muro, Yuko; Buffone, Mariano Gabriel; Okabe, Masaru; Gerton, George L.; Function of the acrosomal matrix: zona pellucida 3 receptor (ZP3R/sp56) Is not essential for mouse fertilization; Society For The Study Of Reproduction; Biology Of Reproduction; 86; 1; 1-2012; 1-6 0006-3363 1529-7268 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/86/1/23,%201-6/2530636 info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.111.095877 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/msword application/pdf |
dc.publisher.none.fl_str_mv |
Society For The Study Of Reproduction |
publisher.none.fl_str_mv |
Society For The Study Of Reproduction |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613439453921280 |
score |
13.070432 |