Different Vancomycin-Intermediate Staphylococcus aureus Phenotypes Selected from the Same ST100-hVISA Parental Strain

Autores
Di Gregorio, Sabrina Noelia; Fernandez, Silvina; Cuirolo, Arabela Ximena; Verlaine, Olivier; Amoroso, Ana; Mengin Lecreulx, Dominique; Famiglietti, Angela María Rosa; Joris, Bernard; Mollerach, Marta Eugenia
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study is to characterize the factors related to peptidoglycan metabolism in isogenic hVISA/VISA ST100 strains. Recently, we reported the increase in IS256 transposition in invasive hVISA ST100 clinical strains isolated from the same patient (D1 and D2) before and after vancomycin treatment and two laboratory VISA mutants (D23C9 and D2P11) selected from D2 in independent experiments. High performance liquid chromatography-mass spectrometry (HPLC-MS) analysis of peptidoglycan muropeptides showed increased proportion of monomeric muropeptides and a concomitant decrease in the proportion of tetrameric muropeptide in D2 and derived mutants when compared to the original strain D1. In addition, strain D2 and its derived mutants showed an increase in cell wall thickness with increased pbp2 gene expression. The VISA phenotype was not stable in D2P11 and showed a reduced autolysis profile. On the other hand, the mutant D23C9 differentiates from D2 and D2P11 in the autolysis profile, and pbp4 transcription profile. D2-derived mutants exhibited differences in the susceptibility to other antimicrobials. Our results highlight the possibility of selection of different VISA phenotypes from a single hVISA-ST100 genetic background.
Fil: Di Gregorio, Sabrina Noelia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Silvina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina
Fil: Cuirolo, Arabela Ximena. Université de Liège; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Verlaine, Olivier. Université de Liège; Bélgica
Fil: Amoroso, Ana. Université de Liège; Bélgica
Fil: Mengin Lecreulx, Dominique. Université Paris Sud; Francia
Fil: Famiglietti, Angela María Rosa. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Joris, Bernard. Université de Liège; Bélgica
Fil: Mollerach, Marta Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
STAPHYLOCOCCUS AUREUS
VANCOMYCIN
hVISA
VIS
MRSA ST100
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47723
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/47723
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Different Vancomycin-Intermediate Staphylococcus aureus Phenotypes Selected from the Same ST100-hVISA Parental StrainDi Gregorio, Sabrina NoeliaFernandez, SilvinaCuirolo, Arabela XimenaVerlaine, OlivierAmoroso, AnaMengin Lecreulx, DominiqueFamiglietti, Angela María RosaJoris, BernardMollerach, Marta EugeniaSTAPHYLOCOCCUS AUREUSVANCOMYCINhVISAVISMRSA ST100https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this study is to characterize the factors related to peptidoglycan metabolism in isogenic hVISA/VISA ST100 strains. Recently, we reported the increase in IS256 transposition in invasive hVISA ST100 clinical strains isolated from the same patient (D1 and D2) before and after vancomycin treatment and two laboratory VISA mutants (D23C9 and D2P11) selected from D2 in independent experiments. High performance liquid chromatography-mass spectrometry (HPLC-MS) analysis of peptidoglycan muropeptides showed increased proportion of monomeric muropeptides and a concomitant decrease in the proportion of tetrameric muropeptide in D2 and derived mutants when compared to the original strain D1. In addition, strain D2 and its derived mutants showed an increase in cell wall thickness with increased pbp2 gene expression. The VISA phenotype was not stable in D2P11 and showed a reduced autolysis profile. On the other hand, the mutant D23C9 differentiates from D2 and D2P11 in the autolysis profile, and pbp4 transcription profile. D2-derived mutants exhibited differences in the susceptibility to other antimicrobials. Our results highlight the possibility of selection of different VISA phenotypes from a single hVISA-ST100 genetic background.Fil: Di Gregorio, Sabrina Noelia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernandez, Silvina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; ArgentinaFil: Cuirolo, Arabela Ximena. Université de Liège; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Verlaine, Olivier. Université de Liège; BélgicaFil: Amoroso, Ana. Université de Liège; BélgicaFil: Mengin Lecreulx, Dominique. Université Paris Sud; FranciaFil: Famiglietti, Angela María Rosa. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Joris, Bernard. Université de Liège; BélgicaFil: Mollerach, Marta Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMary Ann Liebert2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47723Di Gregorio, Sabrina Noelia; Fernandez, Silvina; Cuirolo, Arabela Ximena; Verlaine, Olivier; Amoroso, Ana; et al.; Different Vancomycin-Intermediate Staphylococcus aureus Phenotypes Selected from the Same ST100-hVISA Parental Strain; Mary Ann Liebert; Microbial Drug Resistance: Mechanisms Epidemiology and Disease; 23; 1; 1-2017; 44-501076-6294CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1089/mdr.2016.0160info:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/mdr.2016.0160info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:20:12Zoai:ri.conicet.gov.ar:11336/47723instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:20:12.717CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Different Vancomycin-Intermediate Staphylococcus aureus Phenotypes Selected from the Same ST100-hVISA Parental Strain
title Different Vancomycin-Intermediate Staphylococcus aureus Phenotypes Selected from the Same ST100-hVISA Parental Strain
spellingShingle Different Vancomycin-Intermediate Staphylococcus aureus Phenotypes Selected from the Same ST100-hVISA Parental Strain
Di Gregorio, Sabrina Noelia
STAPHYLOCOCCUS AUREUS
VANCOMYCIN
hVISA
VIS
MRSA ST100
title_short Different Vancomycin-Intermediate Staphylococcus aureus Phenotypes Selected from the Same ST100-hVISA Parental Strain
title_full Different Vancomycin-Intermediate Staphylococcus aureus Phenotypes Selected from the Same ST100-hVISA Parental Strain
title_fullStr Different Vancomycin-Intermediate Staphylococcus aureus Phenotypes Selected from the Same ST100-hVISA Parental Strain
title_full_unstemmed Different Vancomycin-Intermediate Staphylococcus aureus Phenotypes Selected from the Same ST100-hVISA Parental Strain
title_sort Different Vancomycin-Intermediate Staphylococcus aureus Phenotypes Selected from the Same ST100-hVISA Parental Strain
dc.creator.none.fl_str_mv Di Gregorio, Sabrina Noelia
Fernandez, Silvina
Cuirolo, Arabela Ximena
Verlaine, Olivier
Amoroso, Ana
Mengin Lecreulx, Dominique
Famiglietti, Angela María Rosa
Joris, Bernard
Mollerach, Marta Eugenia
author Di Gregorio, Sabrina Noelia
author_facet Di Gregorio, Sabrina Noelia
Fernandez, Silvina
Cuirolo, Arabela Ximena
Verlaine, Olivier
Amoroso, Ana
Mengin Lecreulx, Dominique
Famiglietti, Angela María Rosa
Joris, Bernard
Mollerach, Marta Eugenia
author_role author
author2 Fernandez, Silvina
Cuirolo, Arabela Ximena
Verlaine, Olivier
Amoroso, Ana
Mengin Lecreulx, Dominique
Famiglietti, Angela María Rosa
Joris, Bernard
Mollerach, Marta Eugenia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv STAPHYLOCOCCUS AUREUS
VANCOMYCIN
hVISA
VIS
MRSA ST100
topic STAPHYLOCOCCUS AUREUS
VANCOMYCIN
hVISA
VIS
MRSA ST100
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this study is to characterize the factors related to peptidoglycan metabolism in isogenic hVISA/VISA ST100 strains. Recently, we reported the increase in IS256 transposition in invasive hVISA ST100 clinical strains isolated from the same patient (D1 and D2) before and after vancomycin treatment and two laboratory VISA mutants (D23C9 and D2P11) selected from D2 in independent experiments. High performance liquid chromatography-mass spectrometry (HPLC-MS) analysis of peptidoglycan muropeptides showed increased proportion of monomeric muropeptides and a concomitant decrease in the proportion of tetrameric muropeptide in D2 and derived mutants when compared to the original strain D1. In addition, strain D2 and its derived mutants showed an increase in cell wall thickness with increased pbp2 gene expression. The VISA phenotype was not stable in D2P11 and showed a reduced autolysis profile. On the other hand, the mutant D23C9 differentiates from D2 and D2P11 in the autolysis profile, and pbp4 transcription profile. D2-derived mutants exhibited differences in the susceptibility to other antimicrobials. Our results highlight the possibility of selection of different VISA phenotypes from a single hVISA-ST100 genetic background.
Fil: Di Gregorio, Sabrina Noelia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Silvina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina
Fil: Cuirolo, Arabela Ximena. Université de Liège; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Verlaine, Olivier. Université de Liège; Bélgica
Fil: Amoroso, Ana. Université de Liège; Bélgica
Fil: Mengin Lecreulx, Dominique. Université Paris Sud; Francia
Fil: Famiglietti, Angela María Rosa. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Joris, Bernard. Université de Liège; Bélgica
Fil: Mollerach, Marta Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The aim of this study is to characterize the factors related to peptidoglycan metabolism in isogenic hVISA/VISA ST100 strains. Recently, we reported the increase in IS256 transposition in invasive hVISA ST100 clinical strains isolated from the same patient (D1 and D2) before and after vancomycin treatment and two laboratory VISA mutants (D23C9 and D2P11) selected from D2 in independent experiments. High performance liquid chromatography-mass spectrometry (HPLC-MS) analysis of peptidoglycan muropeptides showed increased proportion of monomeric muropeptides and a concomitant decrease in the proportion of tetrameric muropeptide in D2 and derived mutants when compared to the original strain D1. In addition, strain D2 and its derived mutants showed an increase in cell wall thickness with increased pbp2 gene expression. The VISA phenotype was not stable in D2P11 and showed a reduced autolysis profile. On the other hand, the mutant D23C9 differentiates from D2 and D2P11 in the autolysis profile, and pbp4 transcription profile. D2-derived mutants exhibited differences in the susceptibility to other antimicrobials. Our results highlight the possibility of selection of different VISA phenotypes from a single hVISA-ST100 genetic background.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47723
Di Gregorio, Sabrina Noelia; Fernandez, Silvina; Cuirolo, Arabela Ximena; Verlaine, Olivier; Amoroso, Ana; et al.; Different Vancomycin-Intermediate Staphylococcus aureus Phenotypes Selected from the Same ST100-hVISA Parental Strain; Mary Ann Liebert; Microbial Drug Resistance: Mechanisms Epidemiology and Disease; 23; 1; 1-2017; 44-50
1076-6294
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47723
identifier_str_mv Di Gregorio, Sabrina Noelia; Fernandez, Silvina; Cuirolo, Arabela Ximena; Verlaine, Olivier; Amoroso, Ana; et al.; Different Vancomycin-Intermediate Staphylococcus aureus Phenotypes Selected from the Same ST100-hVISA Parental Strain; Mary Ann Liebert; Microbial Drug Resistance: Mechanisms Epidemiology and Disease; 23; 1; 1-2017; 44-50
1076-6294
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1089/mdr.2016.0160
info:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/10.1089/mdr.2016.0160
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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