Effect of probiotic bacteria isolated from Patagonia on zebrafish gut microbiota and growth

Autores
Garcés, Marisa Elisabeth; Olivera, Nelda Lila; Moris, Mariano; Iglesias, Martín Sebastián; Castaños, Cecilia; Sequeiros, Cynthia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Probiotics are an interesting alternative for sustainable aquaculture. The aim of this study was to assess the effect of probiotic bacteria isolated from Patagonian fish (T4, H16, and TW34) on gut microbiota and growth performance using zebrafish as an experimental model. Assays included one recirculating system (3 tanks) for each probiotic treatment (commercial feed inoculated with one probiotic strain at 1x107 CFU/g) and a control system (only commercial feed). Each tank was randomly stocked with 18 fish, whose weight and length were determined at 0, 15, 30, 60, and 90 days during probiotic treatment. Fish specific growth rate (SGR), condition factor (K), and food conversion ratio (FCR) were also calculated. Total viable bacteria, lactic acid bacteria (LAB), enterobacteria, and Vibrio spp. were quantified by plate-counting to assess the intestinal microbiota at the end of the experiment. As the probiotic strains have antimicrobial activity against fish pathogens, their abundances were detected using the double layer agar method. After 90 days of treatment with T4, H16, or TW34, intestine LAB counts were higher, and Vibrio spp. and enterobacteria lower than those of the control group. Strains T4, H16, or TW34 were recovered from the intestinal microbiota of treated fish (6.8, 7.24, and 7.4 Log10CFU/g of intestine, respectively). At the same time, fish weight of groups fed with T4 or H16 was significantly greater than that of the control. Particularly, fish treated with H16 showed the highest SGR and K, and the lowest FCR, thus its application in aquaculture could be promising.
Fil: Garcés, Marisa Elisabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Olivera, Nelda Lila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Moris, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina
Fil: Iglesias, Martín Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Castaños, Cecilia. Universidad Tecnológica Nacional. Facultad Regional Chubut; Argentina
Fil: Sequeiros, Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina
54th Annual Meeting Agentine Society for Biochemistry and Molecular Biology
Paraná
Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas
Sociedad Española de Bioquímica y Biología Molecular
Materia
ZEBRAFISH
PROBIOTIC
INTESTINAL TRACT
LACTIC ACID BACTERIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/230105
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/230105
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of probiotic bacteria isolated from Patagonia on zebrafish gut microbiota and growthGarcés, Marisa ElisabethOlivera, Nelda LilaMoris, MarianoIglesias, Martín SebastiánCastaños, CeciliaSequeiros, CynthiaZEBRAFISHPROBIOTICINTESTINAL TRACTLACTIC ACID BACTERIAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Probiotics are an interesting alternative for sustainable aquaculture. The aim of this study was to assess the effect of probiotic bacteria isolated from Patagonian fish (T4, H16, and TW34) on gut microbiota and growth performance using zebrafish as an experimental model. Assays included one recirculating system (3 tanks) for each probiotic treatment (commercial feed inoculated with one probiotic strain at 1x107 CFU/g) and a control system (only commercial feed). Each tank was randomly stocked with 18 fish, whose weight and length were determined at 0, 15, 30, 60, and 90 days during probiotic treatment. Fish specific growth rate (SGR), condition factor (K), and food conversion ratio (FCR) were also calculated. Total viable bacteria, lactic acid bacteria (LAB), enterobacteria, and Vibrio spp. were quantified by plate-counting to assess the intestinal microbiota at the end of the experiment. As the probiotic strains have antimicrobial activity against fish pathogens, their abundances were detected using the double layer agar method. After 90 days of treatment with T4, H16, or TW34, intestine LAB counts were higher, and Vibrio spp. and enterobacteria lower than those of the control group. Strains T4, H16, or TW34 were recovered from the intestinal microbiota of treated fish (6.8, 7.24, and 7.4 Log10CFU/g of intestine, respectively). At the same time, fish weight of groups fed with T4 or H16 was significantly greater than that of the control. Particularly, fish treated with H16 showed the highest SGR and K, and the lowest FCR, thus its application in aquaculture could be promising.Fil: Garcés, Marisa Elisabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; ArgentinaFil: Olivera, Nelda Lila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; ArgentinaFil: Moris, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; ArgentinaFil: Iglesias, Martín Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; ArgentinaFil: Castaños, Cecilia. Universidad Tecnológica Nacional. Facultad Regional Chubut; ArgentinaFil: Sequeiros, Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina54th Annual Meeting Agentine Society for Biochemistry and Molecular BiologyParanáArgentinaConsejo Nacional de Investigaciones Científicas y TécnicasSociedad Española de Bioquímica y Biología MolecularTech Science Press2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230105Effect of probiotic bacteria isolated from Patagonia on zebrafish gut microbiota and growth; 54th Annual Meeting Agentine Society for Biochemistry and Molecular Biology; Paraná; Argentina; 2018; 70-711667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/sites/default/files/BIOCELL-SAIB-2018.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:41Zoai:ri.conicet.gov.ar:11336/230105instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:41.375CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of probiotic bacteria isolated from Patagonia on zebrafish gut microbiota and growth
title Effect of probiotic bacteria isolated from Patagonia on zebrafish gut microbiota and growth
spellingShingle Effect of probiotic bacteria isolated from Patagonia on zebrafish gut microbiota and growth
Garcés, Marisa Elisabeth
ZEBRAFISH
PROBIOTIC
INTESTINAL TRACT
LACTIC ACID BACTERIA
title_short Effect of probiotic bacteria isolated from Patagonia on zebrafish gut microbiota and growth
title_full Effect of probiotic bacteria isolated from Patagonia on zebrafish gut microbiota and growth
title_fullStr Effect of probiotic bacteria isolated from Patagonia on zebrafish gut microbiota and growth
title_full_unstemmed Effect of probiotic bacteria isolated from Patagonia on zebrafish gut microbiota and growth
title_sort Effect of probiotic bacteria isolated from Patagonia on zebrafish gut microbiota and growth
dc.creator.none.fl_str_mv Garcés, Marisa Elisabeth
Olivera, Nelda Lila
Moris, Mariano
Iglesias, Martín Sebastián
Castaños, Cecilia
Sequeiros, Cynthia
author Garcés, Marisa Elisabeth
author_facet Garcés, Marisa Elisabeth
Olivera, Nelda Lila
Moris, Mariano
Iglesias, Martín Sebastián
Castaños, Cecilia
Sequeiros, Cynthia
author_role author
author2 Olivera, Nelda Lila
Moris, Mariano
Iglesias, Martín Sebastián
Castaños, Cecilia
Sequeiros, Cynthia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ZEBRAFISH
PROBIOTIC
INTESTINAL TRACT
LACTIC ACID BACTERIA
topic ZEBRAFISH
PROBIOTIC
INTESTINAL TRACT
LACTIC ACID BACTERIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Probiotics are an interesting alternative for sustainable aquaculture. The aim of this study was to assess the effect of probiotic bacteria isolated from Patagonian fish (T4, H16, and TW34) on gut microbiota and growth performance using zebrafish as an experimental model. Assays included one recirculating system (3 tanks) for each probiotic treatment (commercial feed inoculated with one probiotic strain at 1x107 CFU/g) and a control system (only commercial feed). Each tank was randomly stocked with 18 fish, whose weight and length were determined at 0, 15, 30, 60, and 90 days during probiotic treatment. Fish specific growth rate (SGR), condition factor (K), and food conversion ratio (FCR) were also calculated. Total viable bacteria, lactic acid bacteria (LAB), enterobacteria, and Vibrio spp. were quantified by plate-counting to assess the intestinal microbiota at the end of the experiment. As the probiotic strains have antimicrobial activity against fish pathogens, their abundances were detected using the double layer agar method. After 90 days of treatment with T4, H16, or TW34, intestine LAB counts were higher, and Vibrio spp. and enterobacteria lower than those of the control group. Strains T4, H16, or TW34 were recovered from the intestinal microbiota of treated fish (6.8, 7.24, and 7.4 Log10CFU/g of intestine, respectively). At the same time, fish weight of groups fed with T4 or H16 was significantly greater than that of the control. Particularly, fish treated with H16 showed the highest SGR and K, and the lowest FCR, thus its application in aquaculture could be promising.
Fil: Garcés, Marisa Elisabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Olivera, Nelda Lila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Moris, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina
Fil: Iglesias, Martín Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; Argentina
Fil: Castaños, Cecilia. Universidad Tecnológica Nacional. Facultad Regional Chubut; Argentina
Fil: Sequeiros, Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina
54th Annual Meeting Agentine Society for Biochemistry and Molecular Biology
Paraná
Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas
Sociedad Española de Bioquímica y Biología Molecular
description Probiotics are an interesting alternative for sustainable aquaculture. The aim of this study was to assess the effect of probiotic bacteria isolated from Patagonian fish (T4, H16, and TW34) on gut microbiota and growth performance using zebrafish as an experimental model. Assays included one recirculating system (3 tanks) for each probiotic treatment (commercial feed inoculated with one probiotic strain at 1x107 CFU/g) and a control system (only commercial feed). Each tank was randomly stocked with 18 fish, whose weight and length were determined at 0, 15, 30, 60, and 90 days during probiotic treatment. Fish specific growth rate (SGR), condition factor (K), and food conversion ratio (FCR) were also calculated. Total viable bacteria, lactic acid bacteria (LAB), enterobacteria, and Vibrio spp. were quantified by plate-counting to assess the intestinal microbiota at the end of the experiment. As the probiotic strains have antimicrobial activity against fish pathogens, their abundances were detected using the double layer agar method. After 90 days of treatment with T4, H16, or TW34, intestine LAB counts were higher, and Vibrio spp. and enterobacteria lower than those of the control group. Strains T4, H16, or TW34 were recovered from the intestinal microbiota of treated fish (6.8, 7.24, and 7.4 Log10CFU/g of intestine, respectively). At the same time, fish weight of groups fed with T4 or H16 was significantly greater than that of the control. Particularly, fish treated with H16 showed the highest SGR and K, and the lowest FCR, thus its application in aquaculture could be promising.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/230105
Effect of probiotic bacteria isolated from Patagonia on zebrafish gut microbiota and growth; 54th Annual Meeting Agentine Society for Biochemistry and Molecular Biology; Paraná; Argentina; 2018; 70-71
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/230105
identifier_str_mv Effect of probiotic bacteria isolated from Patagonia on zebrafish gut microbiota and growth; 54th Annual Meeting Agentine Society for Biochemistry and Molecular Biology; Paraná; Argentina; 2018; 70-71
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/sites/default/files/BIOCELL-SAIB-2018.pdf
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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