Influence of operational parameters on the fluid-side mass transfer resistance observed in a packed bed bioreactor

Autores
Hussain, Amir; Kangwa, Martin; Abo Elwafa, Ahmed Gad; Fernandez Lahore, Hector
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The influence of mass transfer on productivity as well as the performance of packed bed bioreactor was determined by varying a number of parameters; flow rate, glucose concentration and polymers (chitosan). Saccharomyces cerevisiae cells were immobilized in chitosan and non-chitosan coated alginate beads to demonstrate the effect on external mass transfer by substrate consumption time, lag phase and ethanol production. The results indicate that coating has a significant effect on the lag phase duration, being 30–40 min higher than non-coated beads. After lag phase, no significant change was observed in both types of beads on consumption of glucose with the same flow rate. It was observed that by increasing flow rates; lag phase and glucose consumption time decreased. The reason is due to the reduction of external mass transfer as a result of increase in flow rate as glucose is easily transported to and from the beads surface by diffusion. It is observed that chitosan acts as barrier for transfer of substrate and products, in and out of beads, at initial time of fermentation as it shows longer lag phase for chitosan coated beads than non-coated. Glucose consumption at low flow rate was lower as compared to higher flow rates. The optimum combination of parameters consisting of higher flow rates 30–90 ml/min and between 10 and 20 g/l of glucose was found for maximum production of ethanol.
Fil: Hussain, Amir. Jacobs University; Alemania
Fil: Kangwa, Martin. Jacobs University; Alemania
Fil: Abo Elwafa, Ahmed Gad. Al-Azhar University; Egipto
Fil: Fernandez Lahore, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Jacobs University; Alemania
Materia
ALGINATE
CHITOSAN
EXTERNAL MASS TRANSFER
GLUCOSE
PACKED BED REACTOR
SACCHAROMYCES CEREVISIAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/52139
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/52139
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Influence of operational parameters on the fluid-side mass transfer resistance observed in a packed bed bioreactorHussain, AmirKangwa, MartinAbo Elwafa, Ahmed GadFernandez Lahore, HectorALGINATECHITOSANEXTERNAL MASS TRANSFERGLUCOSEPACKED BED REACTORSACCHAROMYCES CEREVISIAEhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The influence of mass transfer on productivity as well as the performance of packed bed bioreactor was determined by varying a number of parameters; flow rate, glucose concentration and polymers (chitosan). Saccharomyces cerevisiae cells were immobilized in chitosan and non-chitosan coated alginate beads to demonstrate the effect on external mass transfer by substrate consumption time, lag phase and ethanol production. The results indicate that coating has a significant effect on the lag phase duration, being 30–40 min higher than non-coated beads. After lag phase, no significant change was observed in both types of beads on consumption of glucose with the same flow rate. It was observed that by increasing flow rates; lag phase and glucose consumption time decreased. The reason is due to the reduction of external mass transfer as a result of increase in flow rate as glucose is easily transported to and from the beads surface by diffusion. It is observed that chitosan acts as barrier for transfer of substrate and products, in and out of beads, at initial time of fermentation as it shows longer lag phase for chitosan coated beads than non-coated. Glucose consumption at low flow rate was lower as compared to higher flow rates. The optimum combination of parameters consisting of higher flow rates 30–90 ml/min and between 10 and 20 g/l of glucose was found for maximum production of ethanol.Fil: Hussain, Amir. Jacobs University; AlemaniaFil: Kangwa, Martin. Jacobs University; AlemaniaFil: Abo Elwafa, Ahmed Gad. Al-Azhar University; EgiptoFil: Fernandez Lahore, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Jacobs University; AlemaniaSpringer Verlag Berlín2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/52139Hussain, Amir; Kangwa, Martin; Abo Elwafa, Ahmed Gad; Fernandez Lahore, Hector; Influence of operational parameters on the fluid-side mass transfer resistance observed in a packed bed bioreactor; Springer Verlag Berlín; AMB Express; 5; 1; 12-2015; 1-92191-0855CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s13568-015-0111-xinfo:eu-repo/semantics/altIdentifier/url/https://amb-express.springeropen.com/articles/10.1186/s13568-015-0111-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:57:25Zoai:ri.conicet.gov.ar:11336/52139instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:57:25.395CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Influence of operational parameters on the fluid-side mass transfer resistance observed in a packed bed bioreactor
title Influence of operational parameters on the fluid-side mass transfer resistance observed in a packed bed bioreactor
spellingShingle Influence of operational parameters on the fluid-side mass transfer resistance observed in a packed bed bioreactor
Hussain, Amir
ALGINATE
CHITOSAN
EXTERNAL MASS TRANSFER
GLUCOSE
PACKED BED REACTOR
SACCHAROMYCES CEREVISIAE
title_short Influence of operational parameters on the fluid-side mass transfer resistance observed in a packed bed bioreactor
title_full Influence of operational parameters on the fluid-side mass transfer resistance observed in a packed bed bioreactor
title_fullStr Influence of operational parameters on the fluid-side mass transfer resistance observed in a packed bed bioreactor
title_full_unstemmed Influence of operational parameters on the fluid-side mass transfer resistance observed in a packed bed bioreactor
title_sort Influence of operational parameters on the fluid-side mass transfer resistance observed in a packed bed bioreactor
dc.creator.none.fl_str_mv Hussain, Amir
Kangwa, Martin
Abo Elwafa, Ahmed Gad
Fernandez Lahore, Hector
author Hussain, Amir
author_facet Hussain, Amir
Kangwa, Martin
Abo Elwafa, Ahmed Gad
Fernandez Lahore, Hector
author_role author
author2 Kangwa, Martin
Abo Elwafa, Ahmed Gad
Fernandez Lahore, Hector
author2_role author
author
author
dc.subject.none.fl_str_mv ALGINATE
CHITOSAN
EXTERNAL MASS TRANSFER
GLUCOSE
PACKED BED REACTOR
SACCHAROMYCES CEREVISIAE
topic ALGINATE
CHITOSAN
EXTERNAL MASS TRANSFER
GLUCOSE
PACKED BED REACTOR
SACCHAROMYCES CEREVISIAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The influence of mass transfer on productivity as well as the performance of packed bed bioreactor was determined by varying a number of parameters; flow rate, glucose concentration and polymers (chitosan). Saccharomyces cerevisiae cells were immobilized in chitosan and non-chitosan coated alginate beads to demonstrate the effect on external mass transfer by substrate consumption time, lag phase and ethanol production. The results indicate that coating has a significant effect on the lag phase duration, being 30–40 min higher than non-coated beads. After lag phase, no significant change was observed in both types of beads on consumption of glucose with the same flow rate. It was observed that by increasing flow rates; lag phase and glucose consumption time decreased. The reason is due to the reduction of external mass transfer as a result of increase in flow rate as glucose is easily transported to and from the beads surface by diffusion. It is observed that chitosan acts as barrier for transfer of substrate and products, in and out of beads, at initial time of fermentation as it shows longer lag phase for chitosan coated beads than non-coated. Glucose consumption at low flow rate was lower as compared to higher flow rates. The optimum combination of parameters consisting of higher flow rates 30–90 ml/min and between 10 and 20 g/l of glucose was found for maximum production of ethanol.
Fil: Hussain, Amir. Jacobs University; Alemania
Fil: Kangwa, Martin. Jacobs University; Alemania
Fil: Abo Elwafa, Ahmed Gad. Al-Azhar University; Egipto
Fil: Fernandez Lahore, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Jacobs University; Alemania
description The influence of mass transfer on productivity as well as the performance of packed bed bioreactor was determined by varying a number of parameters; flow rate, glucose concentration and polymers (chitosan). Saccharomyces cerevisiae cells were immobilized in chitosan and non-chitosan coated alginate beads to demonstrate the effect on external mass transfer by substrate consumption time, lag phase and ethanol production. The results indicate that coating has a significant effect on the lag phase duration, being 30–40 min higher than non-coated beads. After lag phase, no significant change was observed in both types of beads on consumption of glucose with the same flow rate. It was observed that by increasing flow rates; lag phase and glucose consumption time decreased. The reason is due to the reduction of external mass transfer as a result of increase in flow rate as glucose is easily transported to and from the beads surface by diffusion. It is observed that chitosan acts as barrier for transfer of substrate and products, in and out of beads, at initial time of fermentation as it shows longer lag phase for chitosan coated beads than non-coated. Glucose consumption at low flow rate was lower as compared to higher flow rates. The optimum combination of parameters consisting of higher flow rates 30–90 ml/min and between 10 and 20 g/l of glucose was found for maximum production of ethanol.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/52139
Hussain, Amir; Kangwa, Martin; Abo Elwafa, Ahmed Gad; Fernandez Lahore, Hector; Influence of operational parameters on the fluid-side mass transfer resistance observed in a packed bed bioreactor; Springer Verlag Berlín; AMB Express; 5; 1; 12-2015; 1-9
2191-0855
CONICET Digital
CONICET
url http://hdl.handle.net/11336/52139
identifier_str_mv Hussain, Amir; Kangwa, Martin; Abo Elwafa, Ahmed Gad; Fernandez Lahore, Hector; Influence of operational parameters on the fluid-side mass transfer resistance observed in a packed bed bioreactor; Springer Verlag Berlín; AMB Express; 5; 1; 12-2015; 1-9
2191-0855
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s13568-015-0111-x
info:eu-repo/semantics/altIdentifier/url/https://amb-express.springeropen.com/articles/10.1186/s13568-015-0111-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Verlag Berlín
publisher.none.fl_str_mv Springer Verlag Berlín
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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