eIF3 subunit M regulates blood meal digestion in Rhodnius prolixus affecting ecdysis, reproduction and survival

Autores
Ameijeiras, Pilar; Capriotti, Natalia; Ons, Sheila; Oliveira, Pedro L.; Sterkel, Marcos
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In triatomines, blood-feeding triggers many physiological processes including post embryonic development and reproduction. Different feeding habits, such as hematophagy, can shape gene functions to meet the challenges of each type of diet. The gut of blood-sucking insects faces particular challenges after feeding due to the quantity and the quality of the food ingested. A comparison of transcriptomic and proteomic data indicates that post transcriptional regulation of gene expression is crucial in the triatomine gut. It was proposed that eukaryotic translation initiation factor 3 subunit m (eIF3m) and eIF3e define 2 different eIF3 complexes with a distinct affinity for the different mRNAs, thus selecting the set of mRNAs to be translated and constituting a post transcriptional mode of regulation of gene expression. Because the eIF3m is mainly expressed in the gut, we evaluated its relevance in Rhodnius prolixus physiology through RNA interference-mediated gene silencing. The knockdown of eIF3m reduced the digestion rate, affecting the processes triggered by a blood meal. Its silencing inhibited molting and caused premature death in nymphs while impaired ovary development, oviposition and increased resistance to starvation in adult females. The survival of males after feeding (resistance to starvation) was not affected by eIF3m knockdown. The information regarding the eIF3m function in insects is scarce and the phenotypes observed in R. prolixus upon eIF3m silencing are different and more severe than those previously described in Drosophila melanogaster, indicating a pleiotropic role of this gene in triatomines.
Fil: Ameijeiras, Pilar. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Capriotti, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Fil: Ons, Sheila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Fil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Sterkel, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Materia
EGG HATCHING
HEMATOPHAGOUS VECTORS
LIFESPAN
OVIPOSITION
POST EMBRYONIC DEVELOPMENT
STARVATION RESISTANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/227200
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/227200
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling eIF3 subunit M regulates blood meal digestion in Rhodnius prolixus affecting ecdysis, reproduction and survivalAmeijeiras, PilarCapriotti, NataliaOns, SheilaOliveira, Pedro L.Sterkel, MarcosEGG HATCHINGHEMATOPHAGOUS VECTORSLIFESPANOVIPOSITIONPOST EMBRYONIC DEVELOPMENTSTARVATION RESISTANCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In triatomines, blood-feeding triggers many physiological processes including post embryonic development and reproduction. Different feeding habits, such as hematophagy, can shape gene functions to meet the challenges of each type of diet. The gut of blood-sucking insects faces particular challenges after feeding due to the quantity and the quality of the food ingested. A comparison of transcriptomic and proteomic data indicates that post transcriptional regulation of gene expression is crucial in the triatomine gut. It was proposed that eukaryotic translation initiation factor 3 subunit m (eIF3m) and eIF3e define 2 different eIF3 complexes with a distinct affinity for the different mRNAs, thus selecting the set of mRNAs to be translated and constituting a post transcriptional mode of regulation of gene expression. Because the eIF3m is mainly expressed in the gut, we evaluated its relevance in Rhodnius prolixus physiology through RNA interference-mediated gene silencing. The knockdown of eIF3m reduced the digestion rate, affecting the processes triggered by a blood meal. Its silencing inhibited molting and caused premature death in nymphs while impaired ovary development, oviposition and increased resistance to starvation in adult females. The survival of males after feeding (resistance to starvation) was not affected by eIF3m knockdown. The information regarding the eIF3m function in insects is scarce and the phenotypes observed in R. prolixus upon eIF3m silencing are different and more severe than those previously described in Drosophila melanogaster, indicating a pleiotropic role of this gene in triatomines.Fil: Ameijeiras, Pilar. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; ArgentinaFil: Capriotti, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; ArgentinaFil: Ons, Sheila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; ArgentinaFil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro; BrasilFil: Sterkel, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; ArgentinaWiley Blackwell Publishing, Inc2023-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/227200Ameijeiras, Pilar; Capriotti, Natalia; Ons, Sheila; Oliveira, Pedro L.; Sterkel, Marcos; eIF3 subunit M regulates blood meal digestion in Rhodnius prolixus affecting ecdysis, reproduction and survival; Wiley Blackwell Publishing, Inc; Insect Science; 30; 5; 1-2023; 1282-12921672-9609CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/1744-7917.13174info:eu-repo/semantics/altIdentifier/doi//10.1111/1744-7917.13174info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:00:06Zoai:ri.conicet.gov.ar:11336/227200instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:00:06.597CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv eIF3 subunit M regulates blood meal digestion in Rhodnius prolixus affecting ecdysis, reproduction and survival
title eIF3 subunit M regulates blood meal digestion in Rhodnius prolixus affecting ecdysis, reproduction and survival
spellingShingle eIF3 subunit M regulates blood meal digestion in Rhodnius prolixus affecting ecdysis, reproduction and survival
Ameijeiras, Pilar
EGG HATCHING
HEMATOPHAGOUS VECTORS
LIFESPAN
OVIPOSITION
POST EMBRYONIC DEVELOPMENT
STARVATION RESISTANCE
title_short eIF3 subunit M regulates blood meal digestion in Rhodnius prolixus affecting ecdysis, reproduction and survival
title_full eIF3 subunit M regulates blood meal digestion in Rhodnius prolixus affecting ecdysis, reproduction and survival
title_fullStr eIF3 subunit M regulates blood meal digestion in Rhodnius prolixus affecting ecdysis, reproduction and survival
title_full_unstemmed eIF3 subunit M regulates blood meal digestion in Rhodnius prolixus affecting ecdysis, reproduction and survival
title_sort eIF3 subunit M regulates blood meal digestion in Rhodnius prolixus affecting ecdysis, reproduction and survival
dc.creator.none.fl_str_mv Ameijeiras, Pilar
Capriotti, Natalia
Ons, Sheila
Oliveira, Pedro L.
Sterkel, Marcos
author Ameijeiras, Pilar
author_facet Ameijeiras, Pilar
Capriotti, Natalia
Ons, Sheila
Oliveira, Pedro L.
Sterkel, Marcos
author_role author
author2 Capriotti, Natalia
Ons, Sheila
Oliveira, Pedro L.
Sterkel, Marcos
author2_role author
author
author
author
dc.subject.none.fl_str_mv EGG HATCHING
HEMATOPHAGOUS VECTORS
LIFESPAN
OVIPOSITION
POST EMBRYONIC DEVELOPMENT
STARVATION RESISTANCE
topic EGG HATCHING
HEMATOPHAGOUS VECTORS
LIFESPAN
OVIPOSITION
POST EMBRYONIC DEVELOPMENT
STARVATION RESISTANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In triatomines, blood-feeding triggers many physiological processes including post embryonic development and reproduction. Different feeding habits, such as hematophagy, can shape gene functions to meet the challenges of each type of diet. The gut of blood-sucking insects faces particular challenges after feeding due to the quantity and the quality of the food ingested. A comparison of transcriptomic and proteomic data indicates that post transcriptional regulation of gene expression is crucial in the triatomine gut. It was proposed that eukaryotic translation initiation factor 3 subunit m (eIF3m) and eIF3e define 2 different eIF3 complexes with a distinct affinity for the different mRNAs, thus selecting the set of mRNAs to be translated and constituting a post transcriptional mode of regulation of gene expression. Because the eIF3m is mainly expressed in the gut, we evaluated its relevance in Rhodnius prolixus physiology through RNA interference-mediated gene silencing. The knockdown of eIF3m reduced the digestion rate, affecting the processes triggered by a blood meal. Its silencing inhibited molting and caused premature death in nymphs while impaired ovary development, oviposition and increased resistance to starvation in adult females. The survival of males after feeding (resistance to starvation) was not affected by eIF3m knockdown. The information regarding the eIF3m function in insects is scarce and the phenotypes observed in R. prolixus upon eIF3m silencing are different and more severe than those previously described in Drosophila melanogaster, indicating a pleiotropic role of this gene in triatomines.
Fil: Ameijeiras, Pilar. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Capriotti, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Fil: Ons, Sheila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
Fil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Sterkel, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Endocrinología Experimental y Aplicada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Endocrinología Experimental y Aplicada; Argentina
description In triatomines, blood-feeding triggers many physiological processes including post embryonic development and reproduction. Different feeding habits, such as hematophagy, can shape gene functions to meet the challenges of each type of diet. The gut of blood-sucking insects faces particular challenges after feeding due to the quantity and the quality of the food ingested. A comparison of transcriptomic and proteomic data indicates that post transcriptional regulation of gene expression is crucial in the triatomine gut. It was proposed that eukaryotic translation initiation factor 3 subunit m (eIF3m) and eIF3e define 2 different eIF3 complexes with a distinct affinity for the different mRNAs, thus selecting the set of mRNAs to be translated and constituting a post transcriptional mode of regulation of gene expression. Because the eIF3m is mainly expressed in the gut, we evaluated its relevance in Rhodnius prolixus physiology through RNA interference-mediated gene silencing. The knockdown of eIF3m reduced the digestion rate, affecting the processes triggered by a blood meal. Its silencing inhibited molting and caused premature death in nymphs while impaired ovary development, oviposition and increased resistance to starvation in adult females. The survival of males after feeding (resistance to starvation) was not affected by eIF3m knockdown. The information regarding the eIF3m function in insects is scarce and the phenotypes observed in R. prolixus upon eIF3m silencing are different and more severe than those previously described in Drosophila melanogaster, indicating a pleiotropic role of this gene in triatomines.
publishDate 2023
dc.date.none.fl_str_mv 2023-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/227200
Ameijeiras, Pilar; Capriotti, Natalia; Ons, Sheila; Oliveira, Pedro L.; Sterkel, Marcos; eIF3 subunit M regulates blood meal digestion in Rhodnius prolixus affecting ecdysis, reproduction and survival; Wiley Blackwell Publishing, Inc; Insect Science; 30; 5; 1-2023; 1282-1292
1672-9609
CONICET Digital
CONICET
url http://hdl.handle.net/11336/227200
identifier_str_mv Ameijeiras, Pilar; Capriotti, Natalia; Ons, Sheila; Oliveira, Pedro L.; Sterkel, Marcos; eIF3 subunit M regulates blood meal digestion in Rhodnius prolixus affecting ecdysis, reproduction and survival; Wiley Blackwell Publishing, Inc; Insect Science; 30; 5; 1-2023; 1282-1292
1672-9609
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/1744-7917.13174
info:eu-repo/semantics/altIdentifier/doi//10.1111/1744-7917.13174
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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